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Traditional Chinese medicine pharmacokinetics-pharmacodynamics combined analysis method

A joint analysis and pharmacokinetics technology, applied in the direction of analytical materials, scientific instruments, material separation, etc., can solve the problems that the effects cannot be directly and continuously quantitatively measured, the research and application of PK-PD are limited, and the mechanism of action is not very clear.

Inactive Publication Date: 2011-09-14
ZHEJIANG UNIV
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  • Claims
  • Application Information

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Problems solved by technology

[0003] The current PK-PD combination research and its application have made some progress. However, PK-PD research still faces some problems to be solved. The effect cannot be directly and continuously quantitatively measured; followed by multi-component drugs (such as compound traditional Chinese medicine), which have the characteristics of multi-targets and multi-treatment pathways. The earth limits the research and application of its PK-PD

Method used

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  • Traditional Chinese medicine pharmacokinetics-pharmacodynamics combined analysis method
  • Traditional Chinese medicine pharmacokinetics-pharmacodynamics combined analysis method
  • Traditional Chinese medicine pharmacokinetics-pharmacodynamics combined analysis method

Examples

Experimental program
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Effect test

Embodiment 1

[0020] (1) Take 15 SD rats and randomly divide them into 3 groups: blank group, model group and model administration group. In the model group and the model administration group, the myocardial ischemic disease model was created by ligation of the left anterior descending coronary artery. The model administration group was given Xuesaitong injection, 18mg / kg, subcutaneously in the lower abdomen of the model 7 days after the model was established. Plasma was collected from blank group, model group and model administration group for future use. The spectrum of endogenous metabolites in plasma was determined by GC-MS (see attached figure 1 , where a is the blank group, b is the myocardial ischemic disease model group), first compare the changes in the metabolite profiles of the blank group and the model group, find out the metabolite groups with significant abnormal changes in the model group, and find 24 disease biomarkers, Lactic acid, 3-phosphoglycerol, glyceric acid, succini...

Embodiment 2

[0041] According to the method of Example 1, the difference lies in the PK-PD joint analysis of the component of Xuesaitong injection notoginseng saponin R1

[0042] The plasma concentration-time curve of notoginseng saponin R1 and the time-effect curves of the above seven pharmacodynamic markers were analyzed by Pearson correlation. The correlation coefficient (r) results are shown in Table 3. The negative correlation coefficient shows that the blood concentration of notoginseng saponin R1 has a negative correlation with the regulatory effect on the efficacy markers, that is, the larger the negative correlation coefficient is, the more the callback effect of notoginseng saponin R1 on the corresponding markers plays a major role. Therefore, it can be seen from the table that notoginseng saponin R1 has a callback effect on lactic acid, succinic acid, malic acid, and cholesterol, especially has a strong regulatory effect on lactic acid. It can be seen that R1 is one of the main ...

Embodiment 3

[0045] According to the method of Example 1, the difference lies in the PK-PD joint analysis of ginsenoside Re, a component of Xuesaitong injection

[0046] Pearson correlation analysis was performed on the plasma concentration-time curve of ginsenoside Re and the time-effect curve of the above-mentioned seven pharmacodynamic markers. The correlation coefficient (r) results are shown in Table 4. The negative correlation coefficient shows that the blood concentration of ginsenoside Re has a negative correlation with the regulatory effect on the efficacy markers, that is, the larger the negative correlation coefficient is, the more dominant the callback effect of ginsenoside Re is on the corresponding markers. Therefore, it can be seen from the table that ginsenoside Re has a callback effect on lactic acid, succinic acid, malic acid, and cholesterol, especially has a strong regulatory effect on lactic acid. It can be seen that Re is one of the main medicinal ingredients, and its...

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Abstract

The invention provides a traditional Chinese medicine pharmacokinetics-pharmacodynamics combined analysis method, which determines various endogenous metabolites in blood samples of a blank control group, a model group and a model treatment group by a GC-MS method, uses an abnormally-changed metabolite group of the model group as a biomarker group, uses an effect index with obvious callback of the abnormally-changed metabolite group after traditional Chinese medicine intervention as an efficacy marker group, determines the contents of various drugs in the blood samples of a model animal after single dose by a LC-MS method, draws a plasma concentration-time curve, calculates the pharmacokinetic parameters of each component; determines the amount of the endogenous efficacy marker group in the blood samples of the model animal single dose group by a GC-MS method, draws a time-effect curve, performs PK-PD combined analysis by combining with the plasma concentration-time curve and parameters. The invention reveals the efficacy substance base of traditional Chinese medicine in the treatment of cardiovascular diseases, and provides an effective method for traditional Chinese medicine pharmacokinetics-pharmacodynamics combined analysis.

Description

technical field [0001] The invention belongs to the field of Chinese pharmacy research, and relates to a combined pharmacokinetics-pharmacodynamics (Pharmacokinetics-Pharacodynamics, PK-PD) analysis method of traditional Chinese medicines, in particular to a combined pharmacokinetics-pharmacodynamics analysis method of traditional Chinese medicines for treating cardiovascular diseases , which can be used in the pharmacokinetics-pharmacodynamics joint study of complex traditional Chinese medicines. technical background [0002] The combination of pharmacokinetics and pharmacodynamics can explore the effect of the body on the drug (PK) and the effect of the drug on the body at the same time, revealing the relationship between drug concentration-time-effect, so as to provide clinical safety, effective and rational drug use. Provide scientific experimental basis. [0003] The current PK-PD combination research and its application have made some progress. However, PK-PD research...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 邵青姚宏范骁辉程翼宇张玉峰
Owner ZHEJIANG UNIV
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