32 results about "Acute toxicity testing" patented technology

The invention discloses application of ursolic acid in resisting fish ichthyophthiriasis. The ursolic acid has a remarkable killing effect on ichthyophthirius multifilis in an in-vitro stage, and the 4-hour half effective insecticidal concentration (4h-EC50) of the ursolic acid on a grazing body is 0.3774 mg / L; and the 22-hour half effective insecticidal concentration (22h-EC 50) on the capsule is 4.931 mg / L. An acute toxicity test shows that the 96-hour median lethal concentration (96h-LC50) of ursolic acid to goldfish is 9.358 mg / L, the therapeutic index (TI = 96h-LC50 / 4h-EC50) is up to 24.8, and the safety of ursolic acid is remarkably superior to that of a traditional medicine. Ursolic acid used in the invention is a plant-derived active component, has the characteristics of high environmental compatibility and low biological accumulation, meets the development requirements of green fishery, and has an industrialization basis for being developed into eco-friendly aquatic products.

The invention discloses a pill for treating qi-blood deficiency and a preparation method thereof, and belongs to the technical field of Chinese patent medicine preparation. Comprising 80-90 parts of wall-broken ginseng rootlets, 140-160 parts of freeze-dried Chinese wolfberry fruits, 190-210 parts of fried Chinese yam powder, 110-130 parts of fermented Chinese date powder, 50-70 parts of donkey-hide gelatin beads, 80-100 parts of black sesame seed powder, 45-55 parts of maltitol, 15-25 parts of giant knotweed rhizomes, 10-14 parts of red peony roots and 0.5 part of a compound enzyme preparation. The medicine is small in packaging size, convenient to carry and low in cost; the traditional Chinese medicine composition has the advantages of scientific formula, wide application range, stable process and controllable quality, and embodies the traditional Chinese medicine theory advantages of simultaneous treatment of vital qi and pathogen and simultaneous regulation of qi and blood. Acute toxicity tests show that LD50 is larger than 20 g / kg, and the traditional Chinese medicine is free of liver and kidney dysfunction after being taken for a long time and suitable for long-term conditioning.

The invention discloses bacillus velezensis B116 and a preparation method of the bacillus velezensis B116. The bacillus velezensis B116 is prepared from bacillus velezensis, bacillus velezensis and bacillus velezensis. Belongs to the technical field of microbial technologies and animal probiotic preparations. The bacillus velezensis B116 obtained by screening has relatively strong biofilm forming ability, intestinal toxin-producing escherichia coli inhibiting activity, good self-aggregation and co-aggregation ability, excellent acid resistance, bile salt resistance, high temperature resistance and artificial gastrointestinal fluid characteristics, has no hemolysis and gelatinase activity, and does not carry drug-resistant genes and virulence genes. Animal experiments show that the strain has no acute toxicity to mice, does not influence visceral organ indexes, blood biochemical indexes and intestinal tissue structures, can significantly reduce the death rate of the mice after ETEC challenge and regulate the intestinal inflammatory factor level, has good probiotic effects and safety, and is suitable for development of feed additives or veterinary probiotics or veterinary drug products.

The invention discloses a liposome vaccine adjuvant and a preparation method thereof, and belongs to the technical field of vaccine adjuvants. The adjuvant comprises hydrogenated soybean phospholipids, cholesterol, a saponin adjuvant QS-21, a membrane function lipid and a stabilizer. The preparation method comprises the following steps: dissolving a lipid component in an organic solvent to form a film, hydrating by using a buffer solution containing a stabilizer, carrying out high-pressure homogenization to form blank liposome, purifying, incubating with a QS-21 solution for drug loading, and finally purifying and sterilizing to obtain the product. Through synergistic cooperation of the components and the process, the technical problems of low encapsulation efficiency, poor storage stability, high hemolytic property and the like when QS-21 is encapsulated by traditional liposome are successfully solved, and the prepared liposome vaccine adjuvant passes strict acute toxicity and local irritation tests, is good in biological safety and has good application prospects. The important clinical application value is realized.

The present application relates to the field of environmental monitoring and biosensing technology, and particularly relates to a detection method and system for new pollutants in ecological environment, comprising the following steps: S100: culturing specific effector cells in a biosensing channel, and collecting trans-epithelial electrical resistance (TEER) signals of the effector cells in real time; S200: monitoring a change rate of the TEER signals, and generating an enrichment triggering instruction when the change rate exceeds a preset threshold; S300: in response to the enrichment triggering instruction, introducing a fluid to be tested, which generates the change rate, into a surface-enhanced Raman scattering (SERS) detection cavity, and starting an active enrichment unit to in-situ concentrate the fluid to be tested, so that the volume of the fluid to be tested is contracted to a detection area of a SERS substrate; the present application uses impedance response of effector cells as a preliminary screening "sentinel", realizes targeted capture of only abnormal samples with acute toxicity, avoids ineffective detection of a large number of non-toxic water samples, and significantly improves monitoring efficiency.

The application discloses a branched antibacterial peptide with double fatty acid tails and application thereof, and has the structure of taking Lys as a branched core, using Arg and Trp as two kinds of amino acids to replace the C-terminal of Lys, e -amino, α -amino, to obtain a branched peptide structure. The same length of saturated fatty acid tail chains are introduced into two N-terminal ends of the branched peptide structure to obtain the branched antibacterial peptide with double fatty acid tails, and the structure general formula is Cn-Z-K(Y-Cn)-X, which is marked as XYZn, wherein K is Lys, X, Y and Z are end-point amino acids Arg or Trp, Cn is a saturated fatty acid chain, and n is the number of carbon atoms of the fatty acid chain. The antibacterial peptide structure is novel, simple to synthesize, and has the advantages of broad-spectrum antibacterial activity, low toxicity and high safety in in-vitro antibacterial experiments and toxicity tests. Serum stability experiments show that the antibacterial peptide has excellent stability in mouse serum, and acute toxicity experiments show that the antibacterial peptide has high safety. Therefore, the antibacterial peptide has a good application prospect in preparation of clinical antibacterial drugs.

The invention discloses a 2-(2-phenethyl) chromone compound with COX-2 and 5-LOX dual-targeting inhibitory activity as well as a preparation method and application of the 2-(2-phenethyl) chromone compound, and belongs to the technical field of natural pharmaceutical chemistry. According to the compound, 2-(2-phenethyl) chromone is taken as a skeleton, and a functional group capable of being specifically combined with COX-2 and 5-LOX enzymes is introduced through chemical modification, so that double-target synergistic inhibition on two inflammatory mediators is realized. The invention also provides a synthesis method of the compound, which comprises the following steps: selecting high-purity 2-(2-phenethyl) chromone as an initial raw material, carrying out structural modification under the action of a catalyst, introducing key functional groups such as acrolein and acrylic acid into specific positions of a chromone ring, synthesizing a target compound through a multi-step reaction, and carrying out purification treatment on a reaction product. An in-vitro experiment result shows that IC50 (half maximal inhibitory concentration) of COX-2 is 1.12 [mu] M, and IC50 of 5-LOX is 1.18 [mu] M; and an acute toxicity experiment LD50 is greater than 2000mg / kg.

The application discloses a PET tracer targeting carbonic anhydrase IX (CA IX) and a preparation method and application thereof. 68 The tracer is prepared from a DOTA-bis-sulfonamide precursor compound and GaCl3 reaction. The tracer has high hydrophilicity, excellent stability in vitro and in vivo, low uptake of non-target organs and is mainly excreted through the kidney. Micro-PET / CT imaging shows that the tumor uptake is linearly correlated with the CA IX protein expression level measured by immunohistochemistry. Acute toxicity experiments show that the tracer has good biological safety. The application further provides a radiopharmaceutical composition containing the tracer and application of the tracer in preparation of a PET imaging agent for diagnosing CA IX positive tumors. The tracer has the advantages of high labeling efficiency, low background interference, accurate quantification and potential integration of diagnosis and treatment, and is suitable for precise molecular imaging of CA IX high expression tumors such as clear cell renal cell carcinoma and colorectal cancer.

The invention discloses establishment and application of gobiocypris rarus eye cells, and the gobiocypris rarus eye cells Gbr-eye are constructed by utilizing the eyes of the gobiocypris rarus, and are gobiocypris rarus eye cell lines capable of realizing continuous passage. The culture method of the gobiocypris rarus eye cell line comprises the steps of primary culture, subculture, cryopreservation and recovery. A chemical and environmental water acute toxicity assessment method is established based on the gobiocypris rarus eye cell line, and the method specifically comprises the steps of inoculation of gobiocypris rarus eye cells, exposure of 3, 4-dichloroaniline and environmental water and toxic effect detection of 3, 4-dichloroaniline on the gobiocypris rarus eye cells. According to the establishment method and application of the gobiocypris rarus eye cells, the application of the gobiocypris rarus in toxicity detection of chemicals and environmental water bodies is expanded, and reference is provided for acute toxicity test based on fish cells.

The present application relates to the technical field of biological medicine, in particular to a kind of tricyclic sophoridine compound and its preparation method and application, pharmaceutical composition.The tricyclic sophoridine compound provided in the present application has good potential in preventing and / or treating diseases with lipid metabolic disorder syndrome such as fatty liver disease, diabetes, obesity, etc., and the tricyclic sophoridine compound has low toxicity and high safety.As shown in the test results of examples, the tricyclic sophoridine compound provided in the present application can significantly reduce the body weight of MASLD model mice induced by high-fat feed, improve liver function, glucose-lipid metabolic disorder, insulin resistance index and liver steatosis and other pathological abnormalities, and no toxic side effects are observed for 6 weeks of 100mg / kg dose gavage, and acute toxicity test also shows that it has very high safety.

This invention provides a composite adjuvant, its preparation method, and its application in vaccine preparation, belonging to the field of vaccine adjuvant technology. The composite adjuvant of this invention has low viscosity, only 12 mPa·s at 20°C, ensuring smooth injection; it exhibits high biocompatibility, with no deaths or organ abnormalities in acute toxicity experiments in mice, and no local or systemic adverse reactions or stress responses in chickens after injection; it also demonstrates excellent immunogenicity, achieving a 90% seroconversion rate for Mycoplasma cambogia and an 80% seroconversion rate for Mycoplasma synoviae 28 days after immunization with an inactivated Mycoplasma synoviae vaccine.

The application discloses a liposome vaccine adjuvant and a preparation method thereof, and belongs to the technical field of vaccine adjuvants. The adjuvant comprises hydrogenated soybean phospholipid, cholesterol, saponin adjuvant QS-21, membrane functional lipid and a stabilizer. The preparation method comprises the following steps: dissolving lipid components in an organic solvent to form a film, hydrating the film with a buffer solution containing the stabilizer, forming blank liposomes through high-pressure homogenization, incubating the blank liposomes with a QS-21 solution after purification to load drugs, and finally purifying and sterilizing to obtain the liposome vaccine adjuvant. Through the synergistic cooperation of the above components and the process, the technical problems of low encapsulation efficiency, poor storage stability and high hemolyticity existing in the traditional liposome encapsulation of QS-21 are successfully solved. Moreover, the prepared liposome vaccine adjuvant passes strict acute toxicity and local irritation tests, has good biological safety, and has important clinical application value.

The invention provides application of ciliates in heavy metal toxicity detection and a heavy metal toxicity detection method, and relates to the technical field of heavy metal toxicity evaluation. Specifically, the invention provides application of the Nalklukella marrimilis as a detection organism in heavy metal toxicity detection. The research finds that compared with other marine organisms, the nanokallukella marrimilis has the advantages that the acute toxicity response time of the nanokallukella marrimilis to heavy metals is shorter, and the threshold value is more sensitive. Therefore, the nanokallukella margaritae can be used for detecting the toxicity of the heavy metals, a reference is provided for evaluating the toxicity effect of deep-sea organisms on the heavy metals, and a scientific basis is also provided for evaluating the marine pollution risk. The method is of great significance to environmental protection.

The invention discloses a compound biological enzyme preparation for dispelling effects of alcohol and preventing drunkenness and application thereof, and relates to the technical field of biology. The composite biological enzyme preparation comprises the following raw materials: 3-15 of papain, bromelain, alkaline protease, acid protease, pepsin, trypsin, alpha-amylase, lactase, lipase, cellulase, pectinase, glucoamylase, nuclease, nattokinase and serrapeptase. The composite biological enzyme preparation provided by the invention can obviously prolong the drunkenness latency period and greatly shorten the awakening time; the safety performance is excellent, the acute toxicity test shows that the compound meets the actual non-toxic level standard, and the oral administration safety is high. The alcohol effect dispelling and drunkenness preventing product developed by the invention has the core competitiveness of high efficiency, safety and strong adaptability, and has a wide application prospect.

The invention belongs to the technical field of medicines, and discloses an application of a rhododendron molle toxin VI derivative in analgesia. Specifically, the invention discloses a derivative shown as a formula I or a pharmacodynamically acceptable salt thereof, a pharmaceutical composition containing the derivative or the pharmacodynamically acceptable salt thereof, an application of the compound in preparation of a medicine for preventing or treating pain and an acute toxicity research of the compound.

The application discloses a water pollution event index screening method and device, electronic equipment and a storage medium. The method comprises collecting historical monitoring data sets and real-time monitoring flow data of a water body and preprocessing the data; calculating initial entropy weights of each monitoring index, and screening out a candidate index set with high sensitivity according to a preset threshold; for pollutants in the candidate index set, fusing acute toxicity and biological enrichment risk thereof, constructing a toxicity coefficient and performing normalization processing, and dividing a toxicity grade based on the toxicity coefficient; measuring the correlation strength of the initial entropy weight ordering and the normalized toxicity weight ordering, combining a benchmark toxicity contribution threshold based on a risk threshold and a water body function correction factor calculated based on a water body national standard limit value, and calculating adaptive comprehensive weights of each candidate index; and verifying the decision coefficient of the comprehensive weight and the actual risk entropy to screen out final early warning perception indexes. The application is free of experience parameters, the results are reproducible, and is suitable for various water bodies.

The invention discloses a tumor cell inhibitor as well as a preparation method and application thereof, and belongs to the technical field of medicinal chemistry. The core skeleton of the tumor cell inhibitor is Ph-CH2-C (= O)-NH-Ph '-CH = N-Ph '', the preparation method comprises the two steps of preparing an intermediate A through condensation purification of a 2-aminophenylacetic acid derivative and substituted benzaldehyde and preparing a target compound through reaction purification of the intermediate A and substituted acyl chloride, the steps are simple, the conditions are mild, and the yield is high. Experiments prove that the inhibitor has a remarkable proliferation inhibition effect on tumor cells of liver cancer, lung cancer, breast cancer and the like, dose-dependent inhibition is performed on the growth of transplanted tumors in vivo, the acute toxicity is low, the toxicity to normal cells is small, and the safety is superior to that of cis-platinum. The compound disclosed by the invention can be used for preparing tumor treatment medicines, can be prepared into oral preparations and injection preparations, and has a wide clinical application prospect.

The application discloses a tea processing technology of small yellow flower tea with high active ingredient reservation, and aims at a rare plant, small yellow flower tea, in China. The optimal processing technology is screened out by comparing the influences of different processing technologies on the quality. The processing technology comprises the following steps: selecting small yellow flower tea leaves as raw materials, naturally wilting for 68-76 hours to reduce the water content of the leaves to 20%-30%, drying at 65 DEG C-75 DEG C for 110-130 minutes, and finally crushing and screening through a 50-70 mesh sieve to obtain the small yellow flower tea. The content of tea polyphenol in the small yellow flower tea prepared by the application is greater than or equal to 12.5%, the content of total flavonoids is greater than or equal to 43.0 mg / g, and the content of EGC is greater than or equal to 130 mg / g. The IC50 of the small yellow flower tea to alpha-glucosidase is less than or equal to 60 mu g / mL, and the sodium cholate binding capacity is greater than or equal to 23.5 mu mol / g. The antioxidant, hypoglycemic and hypolipidemic activities of the small yellow flower tea are significantly better than those of other technologies. The acute toxicity test proves that the small yellow flower tea is safe for eating. The application provides a scientific and feasible reference scheme for resource protection and industrialization development of the small yellow flower tea.

The invention relates to a water quality acute toxicity detection device, and belongs to the technical field of water quality toxicity detection devices.The water quality acute toxicity detection device comprises a device body, a detection tank is fixedly connected to the outer wall of the device body, a connecting cylinder is fixedly connected to the top of the detection tank, and a collecting tank is fixedly connected to the outer wall of the connecting cylinder through a connecting pipe; a luminous bacterium storage tank located on one side of the collecting tank is fixedly connected to the outer wall of the connecting cylinder through a connecting pipe, a connecting pipe is slidably connected to the outer wall of the collecting tank, and a sample injection head is fixedly connected to one end of the connecting pipe. According to the requirement of the detection device for water quality toxicity detection characteristics, through the arrangement of the self-adaptive dredging assembly, the situation that when water quality is sampled at a multi-channel sample inlet, the surface of a filter screen is blocked, and the water resource sample introduction rate is affected is reduced, and meanwhile under the impact of water pressure and connection of a reset spring, the water quality toxicity detection effect is improved. And the scraping plate synchronously vibrates, rotates and scrapes the surface of the filter screen, so that the continuity and dredging effect of the detection device on water resource sampling are improved.

The invention discloses an efficient and stable in-vitro cultured calculus bovis preparation process, and belongs to the technical field of biological preparation of traditional Chinese medicines. The process comprises the five steps of genetic engineering compound enzyme liquid preparation, bile pretreatment, bionic enzymatic reaction, crystallization purification and quality regulation and control. The core innovation points are as follows: an engineering strain is constructed through a CRISPR-Cas9 technology to obtain a high-activity compound enzyme solution (the activity is greater than or equal to 80U / mL); an AI intelligent control system is adopted to realize accurate regulation and control, the stability fluctuation of the system is less than or equal to + / -0.5%, and the contamination rate is less than or equal to 0.03%; a composite anti-oxidation system and a supercritical COrefining process are matched, so that the oxidation loss of effective components is reduced. The production period is shortened to 3 days, the finished product bilirubin is larger than or equal to 38.2%, the cholic acid is larger than or equal to 6.6%, the product conforms to the 2025 edition pharmacopoeia standard, acute toxicity is extremely low, skin irritation and sensitization are avoided, the industrialization prospect is good, the product quality is high, safety is high, and natural calculus bovis can be effectively replaced to meet clinical requirements.

The invention belongs to the technical field of medicines, and discloses xyleveratrane diterpenes, a preparation method thereof, a pharmaceutical composition thereof and an application of the xyleveratrane diterpenes. In particular relates to an analgesic active ingredient separated from rhododendron molle G.Don. Flower, a novel xyletre diterpenoid compound 1 and pharmacodynamically acceptable salt thereof, a preparation method of the novel xyletre diterpenoid compound 1, a composition containing the novel xyletre diterpenoid compound 1, an application of the novel xyletre diterpenoid compound 1 in preparation of analgesic drugs and an acute toxicity research of the novel xyletre diterpenoid compound 1 in preparation of the analgesic drugs.

The invention relates to the technical field of health food, and discloses a composition for sobering up, assisting in lowering lipid and improving intestinal tracts and a preparation method, the composition comprises the following components in parts by weight: 40-60 parts of rubus stems and leaves, 3-6 parts of oldenlandia diffusa, 1-3 parts of sculellaria barbata, 0.5-2 parts of balsam pear, 0.5-2 parts of radix bupleuri, 0.5-2 parts of pericarpium citri reticulatae, 0.5-2 parts of elecampane, 0.5-2 parts of raw hawthorn and 0.5-2 parts of semen plantaginis. The preparation method comprises the steps of raw material crushing, enzymolysis extraction, alcohol extraction, water extraction, spray drying and the like. Experimental verification shows that the composition has multiple effects of prolonging the drunkenness latency period, accelerating ethanol metabolism, reducing serum transaminase, regulating blood lipid indexes, improving intestinal peristalsis and flora structures and the like, and acute toxicity tests prove that the composition is high in edible safety. The composition disclosed by the invention can be used for preparing health-care foods with the effects of dispelling the effects of alcohol, assisting in lowering lipid and improving intestinal functions.

The invention discloses establishment and application of gill cells of gobiocypris rarus, gill of gobiocypris rarus is utilized to establish gill cells Gbr-gill of gobiocypris rarus, and the gill cells Gbr-gill are gill cell lines of gobiocypris rarus which can be continuously passed. The culture method of the gobiocypris rarus gill cell line comprises the steps of primary culture, subculture and cryopreservation and resuscitation of the gobiocypris rarus gill cell line. A chemical and environmental water acute toxicity assessment method is established based on the gobiocypris rarus gill cell line, and the method specifically comprises the following steps: inoculating gobiocypris rarus gill cells, exposing 3, 4-dichloroaniline and environmental water, and detecting the toxic effect of 3, 4-dichloroaniline on the gobiocypris rarus gill cells. According to the establishment method and application of gill cells of gobiocypris rarus, the application of gobiocypris rarus in toxicity detection of chemicals and environmental water is expanded, and reference is provided for acute toxicity test based on fish cells.

The application discloses a kind of lactic acid bacteria PH inhibition method water quality online biological toxicity detection method and detection device, wherein detection method includes the following steps: preparation first water sample and second water sample;Get the initial PH voltage value of first water sample and second water sample;Constant-temperature culture is carried out to first water sample and second water sample;Respectively obtain the PH voltage value of first water sample and second water sample after cultivation;Biological toxicity analysis calculation is carried out to water sample to be measured.The method of the application determines the size of harmful substances in water by monitoring the PH change caused by lactic acid, a metabolic product of lactic acid bacteria;Based on the mature glass electrode electrochemical principle method to detect PH, its detection accuracy is not affected by water quality turbidity;The detection method of the application has high consistency with the luminescent bacteria method of prior art for detecting biological toxicity, and meets the related technical requirements in the determination of water quality acute toxicity.

The application discloses a peony endophyte, a method for producing exopolysaccharide by the peony endophyte and application of the exopolysaccharide, and the peony endophyte is obtained by culturing root, stem and leaf parts of peony, and the peony endophyte strain with high exopolysaccharide yield is screened out through color reaction and exopolysaccharide content determination; the strain is identified as Kosakonia cowanii LYH-L3, and the exopolysaccharide yield of the strain is 1.176 mg / mL. Safety determination experiments such as red blood cell hemolysis experiment and zebra fish acute toxicity experiment show that the exopolysaccharide of the application has no irritation and acute toxicity; and through zebra fish model experiment, in-vitro water absorption and water loss rate determination and human body test, it is shown that the exopolysaccharide produced by the peony endophyte strain has a moisturizing effect and a skin barrier repair function, and the application provides a theoretical basis for application of the exopolysaccharide produced by the peony endophyte in preparation of natural moisturizing and repairing cosmetics, skin care products, medicines and other related products, and provides a new target and a new direction for development and utilization of plant resources with Chinese characteristics.

The invention provides a toxicity evaluation method for tetracycline photolysis catalytic products, which is used for performing toxicity evaluation on chronic and acute toxicity of intermediate products generated in the process of catalyzing tetracycline photolysis by a catalyst, and comprises the following steps: constructing a tetracycline geometric structure model according to a foreline molecular orbital theory; based on DFT analysis, ESR analysis and LC-ESI / MS analysis on different sites in a tetracycline geometric structure model, a reasonable degradation path of tetracycline degradation by the catalyst is obtained, and structures of different intermediate products are obtained; aiming at various intermediate products, ECOSAR 2.0 software is adopted to carry out toxicity analysis on fishes, water fleas and green algae, and toxicity results of various intermediate products are obtained through simulation. Intermediate product structures possibly generated in the tetracycline photolysis catalysis process are obtained through simulation analysis, the toxicity of different intermediate products to substances in a water body environment is analyzed, and a comprehensive toxicity analysis result of degradation products in the tetracycline photolysis catalysis process is obtained. And an optimization thought is provided for subsequent water treatment after tetracycline degradation.

The invention belongs to the technical field of medicinal material identification, and particularly relates to application of aesculetin, farrerol and genkwanin to identification of a Korean medicine hepatitis grass wine roasted product. According to the method, thin-layer identification is carried out on the wine-fried product of the Korean medicine hepatitis grass, meanwhile, the contents of water, total ash, acid-insoluble ash, extracts and effective components are detected, and the quality standard of the Korean medicine hepatitis grass is established. In addition, the processing technology of the wine-roasted Korean medicine hepatitis grass is studied, and the optimal wine-roasted technological conditions are screened out. And finally, carrying out acute toxicity experiments on the Korean medicine hedychium japonicum and the wine-fried product thereof, and proving that the Korean medicine hedychium japonicum and the wine-fried product thereof basically have no acute toxicity. In conclusion, the invention provides a theoretical basis for formulating the quality standard of the Korean medicine hepatitis grass and the wine-fried product thereof, and lays a solid foundation for the development of subsequent innovative traditional Chinese medicines and other healthy products.

The disclosure relates to formulations and methods for the in chemico testing of toxins based on a discovery that measuring a reduction in enzyme activity can be used to predict in vivo toxicity, including for example, a skin corrosion, skin irritation, eye corrosion, eye irritation, lung toxicity, liver toxicity, nervous system toxicity, developmental toxicity, acute toxicity etc. Disclosed methods are rapid, easy to perform and shelf-stable approaches for identification of toxic chemicals and materials.

The utility model belongs to the technical field of sewage toxicity exposure and detection, and particularly relates to an adjustable wastewater in-situ biological toxicity exposure and detection zoning device which comprises a detection shell, a partition plate which is detachable from the detection shell is arranged in the detection shell, and the interior of the detection shell is divided into a detection area and a biological chronic toxicity exposure area through the partition plate. An equipment storage box is arranged above one side of the detection shell, an aerator and a heating rod device are symmetrically placed in the storage box, and the aerator and the heating rod device penetrate through wire holes formed in the storage box through wires respectively to be connected with an external power source; the aeration machine conveys oxygen to the bottoms of the concentration detection areas and the biological chronic toxicity exposure areas through hoses, and a heating rod device of the aeration machine transmits a heat source to the corresponding detection areas or the biological chronic toxicity exposure areas through small holes in the bottom of the storage box; according to the utility model, simultaneous exposure of acute toxicity and chronic toxicity of wastewater organisms can be realized, and acute toxicity data and chronic toxicity biological samples can be obtained.

The application discloses a kind of triflufensulfuron-methyl metabolite prediction and preparation method, it is related to pesticide detection technical field, including M-1, M-2 and M-3;Wherein the structural formula of M-1: the structural formula of M-2: the structural formula of M-3: the application further provides the preparation method of triflufensulfuron-methyl main metabolite M-1, M-2 and M-3, the determination of its synthetic method is conducive to further carry out triflufensulfuron-methyl main metabolite in the adsorption / desorption of environment, leaching, migration and conversion environmental behavior research and to aquatic organisms (zebrafish, black shell shrimp), terrestrial animals (red aposhechens earthworm) and amphibian (tadpole) acute toxicity and chronic toxicity mechanism research.