8 results about "Ginsenoside Rf" patented technology

The present application relates to a ginsenoside composition for inhibiting tyrosinase activity, which comprises any two or any three of ginsenoside Rd, ginsenoside Re or ginsenoside Rf. Ginsenoside Rf can be used as a synergistic molecule of ginsenoside Rd and Re, and can significantly enhance the inhibitory ability of tyrosinase activity through synergistic effect, thereby reducing the synthesis and secretion of melanin.

The application discloses application of ginsenoside Rf in preparation of an enhancer of a PD-1 inhibitor antitumor drug, and the ginsenoside Rf has the effects of improving a PD-1 inhibitor antitumor response rate, prolonging a survival period, and reducing toxicity, and has a significant advantage in enhancing PD-1 inhibitor treatment of tumors.

The present application relates to the technical field of compound analysis, and in particular to a quality analysis method of ginseng medicinal materials, comprising the following steps: S100, preparation of a control solution: taking ginsenoside Rg1 control product, ginsenoside Re control product, ginsenoside Rf control product, ginsenoside Rb1 control product, ginsenoside Rc control product, ginsenoside Rb2 control product, ginsenoside Rd control product, 3-O-feruloylquinic acid control product, 4-coumaric acid control product, 8-O-4 / 8-O-4 dehydrogenated tri-ferulic acid control product, and adding methanol to prepare a mixed solution, and shaking to obtain the control solution. The present application synchronously monitors two components of saponins and phenolic acids under one detection method, monitors ten component contents of seven saponin components and three polyphenol components, obtains characteristic spectra of saponin components and polyphenol components, and the monitoring quality is more comprehensive and efficient.

The application provides a multi-component quantitative analysis method of anti-aging tablets, acetonitrile-0.1% formic acid aqueous solution is used as a mobile phase, an HSS T3 chromatographic column is used for target compound separation, and 13 active components in the anti-aging tablets are simultaneously determined in an MRM mode by using an electrospray ionization triple quadrupole mass spectrometer, the linear range of the 13 active components is 0.4-100 ng / mL, the linear range of ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf and ginsenoside Rg1 is 4-1000 ng / mL, the method has high sensitivity, strong specificity and good reproducibility, and realizes simultaneous quantitative analysis of the 13 active components in the anti-aging tablets.

The invention discloses a method for detecting saponin components in ginseng and a preparation thereof. Relates to the field of medicinal material detection. The method for detecting the saponin components in the ginseng and the ginseng preparation comprises the following steps: S1, taking a ginseng sample or a ginseng-containing preparation, adding an extraction solvent, and extracting to obtain a test solution; s2, the test solution is injected into a liquid chromatograph, a chromatographic column is adopted for gradient elution, and octadecylsilane chemically bonded silica is adopted as a filling agent of the chromatographic column; s3, detecting pseudo-ginsenoside F11, ginsenoside Rf, ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Re by adopting an electrospray ionization source negative ion mode and a multi-reaction monitoring mode of a mass spectrometry detector; a mobile phase used for gradient elution is selected from acetonitrile-water, an acetonitrile-ammonium acetate solution, acetonitrile-ammonium formate, methanol-formic acid or an acetonitrile-tetrahydrofuran-ammonium formate solution. By combining C18 chromatographic column gradient elution with a mass spectrum multi-reaction monitoring (MRM) mode and providing a mobile phase system, the problems of narrow applicability, isomeride interference, strong matrix effect and the like of a method for detecting saponin components of ginseng and preparations thereof in the prior art are effectively solved.

This invention belongs to the technical field of Panax notoginseng saponin extraction methods, specifically relating to an optimal method for studying the extraction of Panax notoginseng saponins using different polar solvents based on high-performance liquid chromatography (HPLC) combined with the Kruskal-Wallis H test. The method includes the following steps: S1. Preparation of test sample solution; S2. Preparation of control sample solution; S3. Component detection: HPLC is used to detect saponin components in Panax notoginseng samples; S4. Statistical analysis: The Kruskal-Wallis H nonparametric statistical test is used to perform statistical analysis of inter-group differences in the content of saponin components. This application constructs a research method combining HPLC and the Kruskal-Wallis H nonparametric statistical test, accurately distinguishing the effects of extraction of nine saponin components on their content in different organic solvents, and identifying ginsenoside Rf as a potential quality marker for distinguishing Panax notoginseng samples extracted with different polar solvents. This has significant practical implications for selecting extraction solvents for Panax notoginseng saponins and for conducting in-depth research on the effective components of Panax notoginseng.

This invention belongs to the field of thin-layer chromatography (TLC) identification technology for traditional Chinese medicine, and specifically relates to a rapid TLC identification method and application for American ginseng and ginseng. The TLC identification method includes the following steps: taking a test sample, adding a solvent, sonicating, and taking the supernatant as the test solution; taking American ginseng and ginseng reference materials, adding solvents respectively, sonicating, and taking the supernatant as American ginseng reference material solutions and ginseng reference material solutions; taking ginsenoside Rg1, ginsenoside Rf, ginsenoside Re, ginsenoside Rd, and pseudoginsenoside F11 reference standards, adding solvents, mixing, and obtaining reference solution; finally, performing TLC analysis and result determination on the test solution, reference material solution, and reference solution. This invention is simple to operate, highly efficient and rapid, has good repeatability and high accuracy, and is low in toxicity and durable, and can simultaneously detect adulteration, substitution, and confusion in American ginseng and ginseng.

The application discloses a kind of pharmaceutical composition and preparation method thereof.It is made of the following weight parts raw material components: quercetin 2-5 parts, kaempferol 1-3 parts, isorhamnetin 3-8 parts, isoginkgetin 3-8 parts, ginsenoside Rg1 1-3 parts, ginsenoside Rb1 3-8 parts, ginsenoside Rf 3-8 parts, starch 15-50 parts, calcium carbonate 10-30 parts, water 30-120 parts.The pharmaceutical composition of the application directly uses the main active components in ginseng extract and ginkgo biloba leaf extract.It is found through experimental research that the combination of isorhamnetin and isoginkgetin in the component, the combination of ginsenoside Rb1 and ginsenoside Rf has synergistic effect.