This invention belongs to the technical field of
Panax notoginseng saponin extraction methods, specifically relating to an optimal method for studying the extraction of
Panax notoginseng saponins using different polar solvents based on high-performance liquid
chromatography (HPLC) combined with the Kruskal-Wallis H test. The method includes the following steps: S1. Preparation of
test sample solution; S2. Preparation of
control sample solution; S3. Component detection: HPLC is used to detect
saponin components in
Panax notoginseng samples; S4.
Statistical analysis: The Kruskal-Wallis H nonparametric statistical test is used to perform
statistical analysis of inter-
group differences in the content of
saponin components. This application constructs a research method combining HPLC and the Kruskal-Wallis H nonparametric statistical test, accurately distinguishing the effects of extraction of nine saponin components on their content in different organic solvents, and identifying
ginsenoside Rf as a potential quality marker for distinguishing Panax notoginseng samples extracted with different polar solvents. This has significant practical implications for selecting extraction solvents for Panax notoginseng saponins and for conducting in-depth research on the effective components of Panax notoginseng.