Method for measuring content of five saponin components in red ginseng roots

A method of determination, technology of ginsenosides, applied in measuring devices, material separation, analysis of materials, etc.

Inactive Publication Date: 2017-05-10
河北省药品检验研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ginsenosides are the main effective active ingredients of red ginseng, and are an important standard to measure the internal quality of red ginseng. At present, there are no ginsenoside Rg1, ginsenoside Re, ginsenoside Rf, ginsenoside Rb1 and ginsenoside Ro in red ginseng. Content Determination Method for Simultaneous Determination of Five Kinds of Ginsenosides

Method used

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  • Method for measuring content of five saponin components in red ginseng roots
  • Method for measuring content of five saponin components in red ginseng roots
  • Method for measuring content of five saponin components in red ginseng roots

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Experimental program
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Effect test

Embodiment 1

[0081] Chromatographic conditions and system suitability test column adopts BEH C 18 Chromatographic column; use acetonitrile as mobile phase A, 0.05% phosphoric acid as mobile phase B, and perform gradient elution. The elution gradient is: 0-9 minutes, mobile phase A: 18%→20%; 9-11 minutes, mobile phase A: 20%→28%; 11-40 minutes, the mobile phase A maintains 28%; the detection wavelength is 203nm; the flow rate is 0.4ml / min;

[0082] Preparation of mixed reference solution

[0083] Take an appropriate amount of ginsenoside Rg1 reference substance, ginsenoside Re reference substance, ginsenoside Rf reference substance, ginsenoside Rb1 reference substance, ginsenoside Ro reference substance, weigh them accurately, add methanol to make each 1ml contain 80 μg of ginsenoside Rg1 reference substance, A mixed solution of 60 μg of ginsenoside Re reference substance, 50 μg of ginsenoside Rf reference substance, 150 μg of ginsenoside Rb1 reference substance, and 100 μg of ginsenoside ...

Embodiment 2

[0087] Chromatographic conditions and system suitability test column adopts BEH C 18 Chromatographic column; use acetonitrile as mobile phase A, 0.10% phosphoric acid as mobile phase B, carry out gradient elution, the elution gradient is: 0-9 minutes, mobile phase A: 18%→20%; 9-11 minutes, mobile phase A: 20%→28%; 11-40 minutes, the mobile phase A maintains 28%; the detection wavelength is 203nm; the flow rate is 0.4ml / min;

[0088] Preparation of mixed reference solution

[0089] Take an appropriate amount of ginsenoside Rg1 reference substance, ginsenoside Re reference substance, ginsenoside Rf reference substance, ginsenoside Rb1 reference substance, ginsenoside Ro reference substance, weigh them accurately, add methanol to make each 1ml contain 80 μg of ginsenoside Rg1 reference substance, A mixed solution of 60 μg of ginsenoside Re reference substance, 50 μg of ginsenoside Rf reference substance, 150 μg of ginsenoside Rb1 reference substance, and 100 μg of ginsenoside Ro...

Embodiment 3

[0093] Chromatographic conditions and system suitability test column adopts BEH C 18 Chromatographic column; use acetonitrile as mobile phase A, 0.05% phosphoric acid as mobile phase B, and perform gradient elution. The elution gradient is: 0-9 minutes, mobile phase A: 18%→20%; 9-11 minutes, mobile phase A: 20%→28%; 11-40 minutes, the mobile phase A maintains 28%; the detection wavelength is 203nm; the flow rate is 0.4ml / min;

[0094] Preparation of mixed reference solution

[0095] Take an appropriate amount of ginsenoside Rg1 reference substance, ginsenoside Re reference substance, ginsenoside Rf reference substance, ginsenoside Rb1 reference substance, ginsenoside Ro reference substance, weigh them accurately, add methanol to make each 1ml contain 80 μg of ginsenoside Rg1 reference substance, A mixed solution of 60 μg of ginsenoside Re reference substance, 50 μg of ginsenoside Rf reference substance, 150 μg of ginsenoside Rb1 reference substance, and 100 μg of ginsenoside ...

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Abstract

The invention provides a method for measuring content of five saponin components in red ginseng roots. Ginseng saponin components of main effective active components of the red ginseng roots and are important standards measuring advantages and disadvantages of inner quality of the red ginseng roots. According to the method, ginseng saponin Rg1, ginseng saponin Re, ginseng saponin Rf, ginseng saponin Rb1 and ginseng saponin Ro in the red ginseng roots are simultaneously measured by the aid of an ultra-high performance liquid chromatography, and the method is high in sensitivity, accurate and reliable and can be used for controlling quality of the red ginseng roots.

Description

technical field [0001] The present invention relates to a method for determining the content of ginsenoside components in red ginseng, which belongs to the technical field of traditional Chinese medicine, and specifically relates to a method for determining the content of ginsenoside Rg1, ginsenoside Re, ginsenoside Rf, ginsenoside Rb1 and ginsenoside in red ginseng The content determination method for the simultaneous determination of five components of Ro. Background technique [0002] Red ginseng is the steamed dried roots and rhizomes of Panax ginseng C.A.Mey, a plant of Araliaceae. It is excavated in autumn, washed, steamed, and dried. It is a traditional traditional Chinese medicine with a long history of medicinal use. Ginseng is listed as one of the 36 top grades in "Shen Nong's Materia Medica", and it is also recorded in ancient Chinese medicine classics. According to the scriptures, ginseng: "mainly nourishes the five internal organs, calms the spirit, calms the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 王敏刘永利赵振霞冯丽王钰宁
Owner 河北省药品检验研究院
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