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Method for determining contents of seven types of ginsenosides in healthcare food

A technology for ginsenosides and health food, which is applied in the field of determination of the content of seven ginsenosides, can solve the problems of difficulty in the content of ginsenosides, slow construction of information exchange and emergency response system, and complex components.

Inactive Publication Date: 2011-09-14
PONY TESTING INT GRP SHANGHAI CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the improvement of people's living standards, higher requirements are put forward for the testing of food, especially health food. However, the existing standard system of health food is not perfect, and the construction of information exchange and emergency response system is slow. There is still a large gap between the method and the food technology requirements and food safety requirements, and because of the wide variety of ginsenoside monomers and complex background components, it is necessary for daily detection of ginsenosides Rg1, Re, Rc, Rf, Rb1, Rb2, The content of Rd poses certain difficulties

Method used

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  • Method for determining contents of seven types of ginsenosides in healthcare food

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Embodiment 1

[0025] 1) Take the content of the teabag and grind it into powder, pass through a 20-mesh sieve, accurately weigh 0.2g of the powder into a grinding-mouthed conical flask, and accurately add 35-45ml of ethanol / water solution (volume ratio 1:1), 50 Sonicate at -60°C for 30-60min, add a small amount of acetonitrile after cooling, dilute to 50ml with secondary water, pass through a 0.45μm membrane after centrifugation, and conduct chromatographic analysis on the filtrate.

[0026] 2) Chromatographic analysis was performed using a reversed-phase high-performance liquid chromatography column. The chromatographic column is; Agilent C18 column, 4.6×150mm, 5μm; column temperature: 30°C; injection volume: 10μl; mobile phase: acetonitrile and water.

[0027] a. Prepare mixed standard solutions of ginsenosides Rg1, Re, Rc, Rf, Rb1, Rb2, and Rd at concentrations of 0.01, 0.05, 0.10, 0.20, and 0.50 mg / ml, respectively.

[0028] b. Use gradient elution to effectively separate the target su...

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Abstract

The invention discloses a method for determining contents of seven types of ginsenosides in healthcare food. The seven types of ginsenosides are Rg1, Re, Rc, Rf, Rb1, Rb2 and Rd. The method comprises the steps of: (1) preprocessing a sample; (2) carrying out chromatographic analysis by using a reversed-phase high performance liquid chromatography column; (3) detecting by using an Ultraviolet (UV) detector; (4) determining the compositions of the ginsenosides in the sample according to the retention time of a chromatographic peak; and (5) determining the content of the ginsenosides in the sample by using an external standard method. By means of the method disclosed by the invention, the defects of the traditional detection technology are overcome, the determination of the ginsenoside RF is enhanced, the content of the ginsenosides in the healthcare food can be better analyzed, a basis for better judging American ginseng and ginseng can be provided, and the blank regard to the subject in relevant standards in China is made up.

Description

technical field [0001] The invention relates to a method for determining the content of seven ginsenosides, in particular to a method for determining the content of seven ginsenosides in health food by high performance liquid chromatography. Background technique [0002] Ginsenoside is the main functional component of ginseng. At present, dozens of ginsenoside monomers can be separated and refined through scientific and advanced technology, all of which are composed of aglycone and sugar. Except that the aglycon of Ro is oleanolic acid, Others are protopanaxadiol and protopanaxatriol; among them, the monomeric saponins Rg1 and Rb1 in ginseng are the main components of the intellectual effect in ginseng. In addition, some ginsenosides also have anti-aging, anti-fatigue, regulating central nervous system, activating Skin cells, enhance skin elasticity, reduce wrinkles, etc., and have good effects on diabetes, different types of neurasthenia, and anti-cancer. Ginseng monomeric...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 宋薇
Owner PONY TESTING INT GRP SHANGHAI CO LTD
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