15 results about "Protopanaxatriol" patented technology

The invention discloses a ginsenoside liposome. The ginsenoside liposome comprises ginsenoside, phospholipid and a surfactant, and the ginsenoside comprises protopanoxadiol or / and protopanaxatriol. The invention also discloses a preparation method of the ginsenoside liposome and application of the ginsenoside liposome in preparation of medicines, cosmetics or skin care products. According to the invention, the final desugared product of ginsenoside with the effect of treating skin toxicity reaction is prepared into the liposome, so that the membrane permeation and absorption capabilities of ginsenoside are greatly improved, the skin toxicity caused by tumor treatment can be quickly and effectively relieved, and the drug effect can be played for a long time; the invention provides a new method for treating skin toxic reaction caused by tumor treatment, and makes up for the requirements which cannot be met by the existing treatment means.

The application belongs to the technical field of genetic engineering, and discloses a kind of saccharomyces cerevisiae for producing rare ginsenoside by seaweed biomass and a construction method and application thereof.The saccharomyces cerevisiae has the following characteristics: overexpression of agarase, neojuncanohydrolase, hydroxymethylglutaryl coenzyme A reductase, isopentenyl diphosphate delta isomerase, dammaradienol synthase, protopanaxadiol synthase, cytochrome P450 reductase, protopanaxatriol synthase and glycosyltransferase.The application combines enzymolysis of seaweed biomass with fermentation of rare ginsenoside, which not only endows wild-type yeast with the ability to degrade seaweed biomass that it originally does not have, but also effectively improves the yield of squalene and downstream terpenes (rare ginsenoside Rh1) by overexpression of tHMG1 and IDI1.The saccharomyces cerevisiae can effectively utilize seaweed biomass to obtain squalene and rare ginsenoside Rh1, and has the characteristics of simplicity, economy and ecological friendliness, can convert cheap biomass into high-value products, and provides a way for the development of marine bioeconomy.

The invention provides an application of ginseng oil body in preparation of a medicine for preventing and / or treating colitis, and belongs to the technical field of ginseng seed oil. The detection shows that the oil core structure of the ginseng oil body mainly consists of oleic acid and linoleic acid, and is different from the triglyceride core of the oil body obtained from the existing oil crops. Through lipidomics detection and analysis, the phospholipid monomolecular layer has 335 phospholipids (positive ion mode) and 269 phospholipids (negative ion mode). Through proteomics detection and analysis, 24 kinds of Panax proteins are identified. The condition that the oil core of the ginseng oil body contains protopanaxatriol (PPT) is identified through high performance liquid chromatography-high resolution mass spectrometry. In-vitro detection shows that the ginseng oil body has anti-inflammatory and anti-oxidation effects.

The invention relates to the technical field of microorganisms and functional application, and particularly provides a lactobacillus plantarum crude enzyme as well as a preparation method and application thereof, the lactobacillus plantarum is Lactobacillus plantarum M3 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.26065. The lactobacillus plantarum crude enzyme has the advantages that the lactobacillus plantarum crude enzyme can be used for preparing the lactobacillus plantarum crude enzyme; the lactobacillus plantarum crude enzyme is combined with a low-intensity magnetic field to assist biotransformation of the total ginsenoside to obtain a total ginsenoside transformation product, and the content of protopanaxatriol and rare ginsenoside Rh1, Rg3, Rg5, Rh2 and Rk1 in the total ginsenoside can be increased, and the lipid-lowering activity can be improved.

The application belongs to the field of enzyme engineering and bioinformatics, and discloses a rational design screening method for improving enzyme catalytic performance, a protopanaxatriol synthase mutant and application. The application takes protopanaxatriol synthase CYP716A53v2 (PPTS) as a transformation object, designs and constructs a mutation of a remote site through MM / PBSA guided active site mutation design and conformation bias guided remote site mutation design, and obtains advantage mutants such as T114K, I117D, I117Y, V102S, V102Q and V102N through experimental verification, and further combines to obtain a combined mutant. The application provides a rational design method with small screening amount and high hit rate for activity pocket transformation and remote conformation regulation of a terpene P450 enzyme, and has application value in biosynthesis of rare ginsenosides and other natural products.

The invention is suitable for the technical field of biological medicine, and provides application of 20 (S)-protopanaxatriol in preparation of a medicine for inhibiting tumor metastasis of liver cancer chemotherapy. According to the embodiment of the invention, a molecular mechanism of chemotherapy-induced liver cancer immunosuppression and metastasis increase is analyzed and studied, a key effect of an SHP2-PI3K / AKT signal axis in TAM M2 polarization is disclosed, a novel natural small molecule 20 (S)-protopanaxatriol capable of inhibiting the activity of SHP2 is provided, TAM is blocked from being polarized to an M2 type by inhibiting phosphorylation of SHP2 and activation of a downstream PI3K / AKT signal channel, and the activity of SHP2 is inhibited. Therefore, the tumor immune microenvironment after chemotherapy is improved, the tumor metastasis risk after chemotherapy is reduced, and the overall treatment effect is improved on the premise that safety is guaranteed.

The present application relates to the field of medicine, in particular to a nano delivery system of protopanaxatriol targeting Twist1-Ku70-Sirt1 complex and application of the nano delivery system in treatment of colon inflammation related colorectal cancer, the nano delivery system comprising: (a) protopanaxatriol nanocrystal; (b) lipoic acid modified chitosan; and (c) crosslinking agent; wherein the average particle size of the protopanaxatriol nanocrystal is 100-200 nm; the modification degree of the lipoic acid modified chitosan is 15-25%; the crosslinking agent is sodium tripolyphosphate; and the average particle size of the nano delivery system is 300-500 nm, the solubility and bioavailability of protopanaxatriol are significantly improved by preparing protopanaxatriol into nanocrystal.

The invention belongs to the technical field of plant extraction, and particularly discloses a preparation method and application of a ginseng extract containing protopanaxatriol. By adopting a new processing technology, the content of rare saponin is increased, and meanwhile, the flavor of the conventional ginseng extracting solution is greatly improved. Animal experiment results prove that the ginseng extracting solution remarkably improves the sleep improving effect of ginseng. In addition, the method disclosed by the invention has the advantages of high product purity, simple process, low cost, environmental protection and the like, and has a wide application prospect in the field of ginseng extraction.

The invention belongs to the technical field of genetic engineering, and discloses saccharomyces cerevisiae for producing rare ginsenoside by utilizing seaweed biomass as well as a construction method and application of the saccharomyces cerevisiae. The saccharomyces cerevisiae has the following characteristics: overexpression agarase, neoagarobiose hydrolase, hydroxymethyl glutaryl coenzyme A reductase, isopentenyl diphosphate delta isomerase, dammarendiol synthase, protopanoxadiol synthase, cytochrome P450 reductase, protopanaxatriol synthase and glycosyl transferase. According to the invention, seaweed biomass enzymolysis and rare ginsenoside fermentation are combined, so that wild yeast is endowed with the capability of degrading seaweed biomass, which does not exist originally; the overexpression of tHMG1 and IDI1 effectively increases the yield of squalene and downstream terpenoids (rare ginsenoside Rh1). The Saccharomyces cerevisiae can effectively utilize seaweed biomass to obtain squalene and rare ginsenoside Rh1, does not have the characteristics of simplicity, economy and eco-friendliness, can convert cheap biomass into products with high added value, and provides a way for developing marine biological economy.

The invention discloses a gynostemma pentaphyllum glycosyl transferase GpUGT74A1 and application thereof, and relates to the technical field of biology, and the amino acid sequence of the gynostemma pentaphyllum glycosyl transferase GpUGT74A1 is as shown in SEQ ID NO.3. The gynostemma pentaphyllum glycosyl transferase GpUGT74A1 has the advantages that the gynostemma The invention provides a brand-new gynostemma pentaphyllum glycosyl transferase, and the gynostemma pentaphyllum glycosyl transferase catalyzes the 20th hydroxyl of protopanaxatriol PPT and protopanoxadiol PPD to form glycosidic bonds with glucose molecules, so that ginsenoside F1 and ginsenoside CK are respectively generated. Compared with a plurality of known gynostemma pentaphyllum glycosyl transferases, the gynostemma pentaphyllum glycosyl transferases have the highest homology of UGTGp5, and the homology is 36.32%. When the gynostemma pentaphyllum glycosyl transferase is used for preparing ginsenoside F1 and ginsenoside CK, the method and the process are simple, the yield is high, and the consumption is low. The current glycosyl transferase database is enriched.

The invention discloses a novel oil-soluble micelle preparation. The component A comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, cardiolipin, cephalin and dipalmitoyl phosphatidylcholine, and the component B comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, phosphatidylcholine, cephalin and dipalmitoyl phosphatidylcholine; one or more of dipalmitoyl phosphatidyl ethanolamine and dipalmitoyl phosphatidyl ethanolamine; the component B comprises a component B1 and a component B2, and the component B1 comprises one or more of phytosterol, cholesterol, ergosterol, protopanoxadiol, protopanaxatriol, ganoderic acid, phytol, isophytol, retinol, andrographolide, paclitaxel, artemisinin and bisabolol; and the component B2 is prepared from one or more of 1, 2-hexanediol, ethylhexylglycerin, cyclohexyl glycerin, absolute ethyl alcohol and isosorbide dimethyl ether. The key point of the invention is to provide a low-cost formula and a low-cost preparation method for preparing the oil-soluble micelle preparation.

The invention is applicable to the technical field of biological medicines, and provides application of 20 (S)-protopanaxatriol in preparation of a medicine for reversing chemotherapy resistance of liver cancer. The invention provides application of 20 (S)-protopanaxatriol (20 (S)-protopanaxatriol, hereinafter referred to as 20S-PPT) which is derived from ginseng in preparation of drugs for reversing chemotherapy resistance of liver cancer, the sensitivity of liver cancer cells to chemotherapy drugs is improved, the chemotherapy-induced apoptosis effect is enhanced, and the in-vivo anti-tumor effect is improved on the premise that safety is guaranteed.

The present invention belongs to the field of plant extraction technology and specifically discloses a method for preparing a ginseng extract containing protopanaxatriol and its application. This invention utilizes a novel processing technique to increase the content of rare saponins while significantly improving the flavor of conventional ginseng extracts. Animal experimental results demonstrate that the ginseng extract of the present invention significantly enhances the sleep-improving efficacy of ginseng. Furthermore, the method of the present invention offers advantages such as high product purity, simple process, low cost, and environmental friendliness, and has broad application prospects in the field of ginseng extraction.

The present application belongs to the technical field of medicine and pharmacy, and in particular relates to an application of proto-prosolan in preparation of a Streptococcus suis type 2 capsule inhibitor. The present application discloses that proto-prosolan can significantly weaken the pathogenicity and immune escape ability of Streptococcus suis type 2 by inhibiting capsule synthesis, and then effectively plays an anti-infection role. Compared with vaccines, antibodies and phage enzymes and other highly limited anti-capsule strategies specific to serotypes, proto-prosolan, as an anti-toxicity inhibitor of Streptococcus suis type 2, has potential advantages such as broad spectrum, and has important scientific significance and application value for the research and development of new anti-drugs in the future. The proto-prosolan can be applied to the preparation of a Streptococcus suis type 2 capsule inhibitor and a drug for preventing and treating Streptococcus suis type 2 infection.

The invention relates to the field of medicine, in particular to a protopanaxatriol nanometer delivery system targeting a Twist1-Ku70-Sirt1 compound and application of the nanometer delivery system in treatment of colorectal cancer related to colitis, and the nanometer delivery system comprises: (a) a protopanaxatriol nanometer crystal; (b) chitosan modified by lipoic acid; and (c) a cross-linking agent; wherein the average particle size of the protopanaxatriol nanocrystal ranges from 100 nm to 200 nm; the modification degree of the lipoic acid modified chitosan is 15 to 25 percent; the cross-linking agent is sodium tripolyphosphate; the average particle size of the nano delivery system is 300-500 nm, and the solubility and bioavailability of the nano delivery system are remarkably improved by preparing the protopanaxatriol into nano crystals.