33 results about "Ginsenoside CK" patented technology

The invention relates to the technical field of gene engineering and enzyme engineering, in particular to a beta-glucosidase mutant with high catalytic activity and application of the beta-glucosidase mutant. The sequence of the beta-glucosidase mutant is obtained by carrying out any one of the following mutations on an amino acid sequence as shown in SEQ ID No.1: respectively mutating proline at the 95th site and arginine at the 178th site into cysteine; phenylalanine at the 98th site and arginine at the 178th site are respectively mutated into cysteine. The obtained mutants P95C / R178C and F98C / R178C have high enzyme activity, ginsenoside Rb1 can be converted into CK, the conversion efficiency is respectively improved by 83.52% and 105.79% compared with that of wild beta-glucosidase, and the industrial application of enzymatic conversion of rare ginsenoside CK is promoted.

The present patent application discloses an antidepressant composition containing rare ginsenosides and a preparation method thereof. The antidepressant composition of the present application contains ginseng hydrolysate, Zizyphus jujuba powder and gamma-aminobutyric acid and other functional ingredients, wherein the ginseng hydrolysate is rich in rare ginsenoside CK. The antidepressant composition of the present application has good antidepressant efficacy, can significantly increase the content of 5-hydroxytryptophan (5-HT) and gamma-aminobutyric acid (GABA) in brain tissue, achieve better enhancement of emotional stability and cognitive flexibility, improve anhedonia, relieve anxiety comorbidity and sleep disorders, provide a steady-state foundation for emotional recovery, and improve the efficacy of antidepressants. At the same time, the antidepressant composition of the present application has no side effects on the human body, the product has high safety, the preparation method is simple, the quality is controllable, and the industrialized continuous production can be realized.

The invention relates to an anti-wrinkle composition, application thereof and cosmetics, the anti-wrinkle composition comprises ginsenoside CK and low-molecular-weight sodium hyaluronate, the mass ratio of the ginsenoside CK to the low-molecular-weight sodium hyaluronate is 1: (5-100), and the molecular weight of the low-molecular-weight sodium hyaluronate does not exceed 5000 Daltons. The anti-wrinkle composition has the beneficial effects that the usage amount of Ectoine and rare ginsenoside CK can be reduced, so that the cost is saved, and meanwhile, an excellent anti-wrinkle effect is realized.

The present application relates to the technical field of nanomedicine, in particular to a β-cyclodextrin-carboxymethyl chitosan-deoxycholic acid nanoparticle carrying ginsenoside CK, a preparation method and application. A first esterification reaction is performed between a β-cyclodextrin solution and a carboxyl-activated carboxymethyl chitosan solution to obtain a β-cyclodextrin-carboxymethyl chitosan polymer; a second esterification or amidation reaction is performed by adding carboxyl-activated deoxycholic acid to the β-cyclodextrin-carboxymethyl chitosan polymer to obtain a β-cyclodextrin-carboxymethyl chitosan-deoxycholic acid polymer; an ethanol solution of ginsenoside CK is mixed with a water solution of the β-cyclodextrin-carboxymethyl chitosan-deoxycholic acid polymer to obtain a whole particle, and a freeze-drying protective agent is added for freeze-drying to obtain the β-cyclodextrin-carboxymethyl chitosan-deoxycholic acid nanoparticle carrying ginsenoside CK. The present application focuses on the design of an intelligent carrier, realizes the synergistic optimization of high drug loading and intelligent delivery, and overcomes the defects of the prior art.

This invention discloses a β-glucosidase, its preparation method, and its applications, relating to the field of genetic engineering technology. The amino acid sequence of the β-glucosidase is shown in SEQ ID NO:1. The nucleotide sequence encoding this enzyme, optimized for codon bias in *E. coli*, is shown in SEQ ID NO:2. The constructed recombinant expression vector contains an IPTG inducible promoter, a ribosome binding site, a 6×His tag, a TEV protease recognition site, a flexible linker peptide, and the sequence shown in SEQ ID NO:2. The recombinant expression vector is transformed into *E. coli*, and after induction expression, high-density fermentation, and affinity purification, a highly soluble and active target enzyme is obtained. This enzyme can efficiently catalyze the conversion of ginsenoside Rb1 to rare ginsenoside CK, with a conversion rate exceeding 82%. This invention improves the soluble expression level and preparation efficiency of the target enzyme, making it suitable for industrial production.

Compositions with weight-loss effects and their applications belong to the field of biomedicine. To improve the fat-reducing effect and further solve the off-target effect problem caused by existing weight-loss mechanisms, the composition includes fucoxanthin 0.8%~2.5%, ginsenoside CK 0.6%~1.2%, black ginseng active peptide 0.3%~0.6%, amylase inhibitor 12%~30%, prickly pear cactus extract 6%~15%, allicin 0.2%~0.6%, and excipients. This invention achieves weight regulation through comprehensive synergy of multiple targets.

The invention provides a bacillus safensis Sys-Gs-001 strain for producing ginsenoside CK, the strain is preserved in the China Center for Type Culture Collection on November 24, 2025, the preservation number is CCTCC NO: M20252681, the preservation number is CCTCC NO: M20252681, the preservation number is CCTCC NO: M20252681, and the preservation number is CCTCC NO: M20252681. The 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the strain is as shown in SEQ ID NO. 1. The bacillus safensis Sys-Gs-001 strain provided by the invention can produce ginsenoside CK through metabolism, can be subjected to large-scale fermentation culture in a short time, is relatively low in fermentation cost, is not limited by conditions such as time domains and seasons, and has remarkable advantages. Meanwhile, the invention also provides optimized microbial fermentation conditions and a formula of the culture medium, and the yield of the ginsenoside CK is relatively high.

The invention relates to the technical field of supramolecules, in particular to a ginsenoside CK-aescin supramolecule, in the supramolecule, ginsenoside CK and aescin are formed through a non-covalent bond, the mass ratio of ginsenoside CK to aescin is 1: (1-25), and a preparation method comprises the following steps: dispersing aescin in an organic solvent aqueous solution to prepare a suspension A, dissolving ginsenoside CK in ethanol to prepare a solution D for later use; stirring the suspension A to obtain a suspension B; performing ultrasonic treatment on the suspension B to obtain emulsion C; slowly adding the emulsion C into the solution D while stirring, stirring to obtain a ginsenoside CK-aescin supramolecular solution crude product, and dialyzing. According to the ginsenoside CK-aescin supramolecule prepared by the invention, an IVPT test shows that the transdermal absorption capacity of the ginsenoside CK-aescin supramolecule is remarkably improved, so that the ginsenoside CK-aescin supramolecule can be better absorbed by human skin, and the efficacy of the ginsenoside CK-aescin supramolecule is favorably exerted.

This patent application discloses a rare ginsenoside functional composition and its preparation method. The rare ginsenoside functional composition of this invention comprises active ingredients such as ginseng enzymatic hydrolysate and ginseng fermentation product, wherein the ginseng enzymatic hydrolysate is rich in rare ginsenoside CK, and the ginseng fermentation product is rich in ginsenosides Rk1, Rg5, Rk3, and Rh4. The rare ginsenoside functional composition of this invention has good effects in relieving mental stress and antidepressant effects.

The invention relates to the technical field of cosmetics, in particular to a ginsenoside CK nanoscale bilayer inclusion as well as a preparation method and application thereof. According to the invention, the stability problem of rare ginsenoside CK applied to cosmetics is solved through a nano-scale bilayer wrapping technology, and the stability and bioavailability of ginsenoside CK are improved. The preparation method of the inclusion provided by the invention is simple, can realize mass production, and is safe and stable, and the prepared ginsenoside CK nano-scale bilayer inclusion can be suitable for various dosage forms of cosmetics.

The present application belongs to the field of biological medicine, and relates to application of ginsenoside CK in preparation of a porcine oocyte damage inhibitor. The present application aims to explore the influence of CK on in-vitro maturation of porcine oocytes induced by BaP and the embryo development capacity thereof. The research of the present application finds that adding CK can improve in-vitro maturation of porcine oocytes and subsequent embryo development after damage induced by BaP by increasing the antioxidant activity of oocytes and improving the function of mitochondria.

The invention provides a hair disulfide bond repairing composition and application thereof, and relates to the technical field of cosmetics, the hair disulfide bond repairing composition comprises ginsenoside CK, a recombinant collagen composition and multiple hydrolyzed protein, the recombinant collagen composition comprises small-molecule recombinant collagen, middle-molecule recombinant collagen and large-molecule recombinant collagen, and the multiple hydrolyzed protein comprises hydrolyzed vegetable protein, hydrolyzed sericin, hydrolyzed wheat protein, hydrolyzed soybean protein and hydrolyzed corn protein. The ginsenoside CK, the recombinant collagen composition and the multi-hydrolyzed protein are combined for use, so that the antioxidant capacity of the composition reaches the highest level, active oxygen can be removed to the greatest extent to prevent new fracture of disulfide bonds, the capacity of repairing the disulfide bonds can reach the highest, and the effect of repairing the disulfide bonds is achieved. The problem that hair is easy to break due to factors such as dyeing, perming and high temperature is fundamentally solved.

The invention discloses a beta-glucosidase BglSK mutant and an application of the beta-glucosidase BglSK mutant. The BglSK mutant is mutated by taking an amino acid sequence as shown in SEQ ID NO. 1 as a benchmark. According to the beta-glucosidase BglSK mutant disclosed by the invention, one or more amino acid residues are introduced at a specific site for substitution, so that the thermal stability and / or catalytic activity of beta-glucosidase are / is remarkably improved; in addition, ginsenoside Rd or analogues thereof can be efficiently catalyzed, and rare ginsenoside F2 and / or rare ginsenoside CK can be directionally generated. According to the beta-glucosidase BglSK mutant disclosed by the invention, large-scale production of rare ginsenoside is successfully realized, and the beta-glucosidase BglSK mutant has huge industrial application prospect and value.

The invention relates to application of ginsenoside CK in preparation of drugs for treating diseases by activating PPAR gamma and / or CPT1A pathways, and belongs to the technical field of medicines.The action mechanism of ginsenoside CK in prevention or treatment of metabolism-related fatty liver diseases (MAFLD) is obtained for the first time through research, and by establishing an MAFLD mouse model and an MAFLD cell model respectively, the action mechanism of ginsenoside CK in prevention or treatment of metabolism-related fatty liver diseases is obtained. Through PPAR gamma and CPT1A inhibition and ginsenoside CK intervention tests, the application of ginsenoside CK in remarkably reducing expression of ACC1 / FAS / SREBP1c, improving expression of CPT1 / CPT2, reducing serum TG / TC / FFA / LDL-C / ALT / AST level, increasing HDL-C, reducing hypoglycemia / insulin level and insulin resistance index, reducing ROS accumulation of hepatocytes, improving mitochondrial membrane potential, improving mitochondrial membrane potential, reducing liver cancer, reducing liver cancer, reducing liver cancer, reducing liver cancer, reducing liver cancer, reducing liver cancer and reducing liver cancer. The invention relates to a mechanism for preventing or treating MAFLD by inhibiting an NF-kappa B / JNK inflammation pathway and the like, and provides a new candidate component for research and development of MAFLD treatment drugs.

The invention discloses a chemical composition for regulating scalp ecology and a preparation method and application, the chemical composition comprises a barrier repairing component, an oil control regulating component and an anti-dandruff micro-ecological component, and the barrier repairing component comprises an agilawood extract and an African amomum kravanh seed extract; the oil-control adjusting component comprises an orchid / leaf / stem extract, ginsenoside CK and a red clover extract; the anti-dandruff micro-ecological component comprises a crocus sativus extract, hydrolyzed silk, saccharomycetes / rice fermentation product filtrate, saccharomycetes / barley seed fermentation product filtrate and a lactic acid bacteria fermentation product. According to the composition disclosed by the invention, a key signal channel is activated and an inflammatory factor repair barrier is regulated through PPARalpha; the sebum metabolism is regulated from multiple pathways of hormone signal, lipid synthesis, cell differentiation and inflammatory environment AMPK-PPARgamma-AR-ACC-COX2 (AMPK-PPARgamma-AR-ACC-COX2); the healthy scalp micro-ecology is reconstructed through the strategy of'bacteriostasis-bacteria cultivation-physical protection '.

The invention relates to the technical field of bioengineering, in particular to recombinant glucoside hydrolase and application thereof in preparation of rare ginsenoside CK. Compared with glucoside hydrolase with an amino acid sequence shown as SEQ ID NO: 1, the recombinant glucoside hydrolase has R234K; and having one or more of R233K and W288Y, as well as one or more of R233K and W288Y. The recombinant glucoside hydrolase can be used for efficiently and specifically preparing rare ginsenoside CK, and is high in conversion rate and suitable for industrial production.

The application relates to the field of biotechnology, and particularly discloses a mutant strain Cbg61-T37, a co-expression strain Cbg61-T37-MPR1 and a construction method and application thereof. The mutant strain Cbg61-T37 is preserved in the China General Microbiological Culture Collection Center on August 18, 2025, has a preservation number of CGMCC No. 35649, and is classified and named as Pichia pastoris. Pichia pastoris The mutant strain Cbg61-T37 provided by the application has an enzyme hydrolysis activity of 62.9 U / mg, which is increased by 43.8% compared to the original strain. Therefore, the mutant strain Cbg61-T37 is more conducive to catalysis and synthesis of the rare ginsenoside CK.

The application discloses a beta-glucosidase BglSK mutant and application thereof. The BglSK mutant is mutated based on the amino acid sequence shown in SEQ ID NO. 1. The beta-glucosidase BglSK mutant of the application significantly improves the thermal stability and / or catalytic activity of the beta-glucosidase by introducing one or more amino acid residue substitutions at specific sites. In addition, the beta-glucosidase BglSK mutant can efficiently catalyze ginsenoside Rd or an analogue thereof to direct generation of rare ginsenoside F2 and / or rare ginsenoside CK. The beta-glucosidase BglSK mutant of the application successfully realizes large-scale production of rare ginsenosides, and has great industrial application prospect and value.

The invention relates to the technical field of supramolecule preparation, in particular to a ginsenoside CK-resveratrol supramolecule which comprises ginsenoside CK and resveratrol which are connected through a hydrogen bond, and in an XRD (X-Ray Diffraction) spectrum of the supramolecule, a characteristic peak exists at the position where a 2theta angle is 15.89 degrees; the preparation method comprises the following steps: mixing ginsenoside CK and resveratrol powder, and dissolving the mixture in a DMSO or ethanol solvent to obtain a solution A; dropwise adding the solution A into purified water; then carrying out magnetic stirring to obtain a ginsenoside CK-resveratrol supramolecular crude product; and dialyzing the supermolecule crude product to obtain the ginsenoside CK-resveratrol supermolecule aqueous solution. The transdermal absorption capacity of the ginsenoside CK-resveratrol supramolecule prepared by the preparation method disclosed by the invention is remarkably improved.

The invention provides a method for preparing ginsenoside CK through a multi-enzyme system, and relates to the field of biological enzyme method preparation of active substances. The method comprises the following steps: constructing genetically engineered bacteria capable of heterologously expressing two glucosidase, wherein a synthetic gene sequence of glucosidase A is selected from a gene sequence of glucosidase of Alaska sphingosine box bacteria; the synthetic gene sequence of the glucosidase B is selected from the gene sequence of glucosidase of paenibacillus mucilaginosus; the glucosidase A is used for converting Rb1, Rb2 and Rc in a substrate into Rd and converting Rd into F2, and the glucosidase B is used for converting F2 into CK; after two recombinant bio-enzymes are obtained through fermentation, a raw material containing diol ginsenoside is used as a substrate for fermentation and enzymolysis; and heating, filtering, removing enzyme, cooling and crystallizing. The method is low in raw material dependence, high in utilization rate, short in conversion time, high in conversion efficiency, high in product purity after conversion, free of separation and purification operation and suitable for large-scale industrial production.

The application discloses a melanin-regulated autophagy-inflammation-melanin whitening synergistic composition and application thereof. The composition comprises ginsenoside CK, rhodioside and lognning, and the molar concentration ratio of the three is 1:(0.3-0.8):(0.05-0.4). The three synergistically reduce tyrosinase activity and melanin content, relieve oxidative stress, up-regulate Beclin-1 and LC3II expression to induce melanocyte autophagy, and inhibit the expression of TNF-alpha, IL-6, IL-1beta, COX-2, iNOS and NF-kappa B p65 inflammation-related factors. The application realizes multi-target synergistic regulation of autophagy-inflammation-melanogenesis, reduces inflammation-induced melanin formation, and is suitable for preparing skin external preparations with whitening, antioxidant and anti-inflammatory effects.

The present application belongs to the field of bioengineering technology, and specifically discloses a continuous bio-manufacturing system and method for rare ginsenoside CK, which loads immobilized biological catalyst in a gas-lift loop reactor, continuously feeds substrate solution for enzymatic conversion, and sequentially passes the reaction solution through a tubular ultrafiltration membrane and a flat-plate nanofiltration membrane, so as to retain the catalyst and unconverted substrate in the tubular ultrafiltration membrane and return them to the reactor, and retain and concentrate the product CK in the flat-plate nanofiltration membrane online, and then collect the liquid through acid precipitation, water washing, microfiltration, concentration and drying to obtain CK dry powder. The present application returns most of the concentrated liquid to the reaction kettle to maintain a suitable reaction driving force, while collecting a small part of CK and removing it from the system in time, so that the CK concentration in the reactor is always maintained below the preset threshold, which fundamentally eliminates the product feedback inhibition and improves the conversion rate of ginsenoside Rb1 to CK.

The application belongs to the technical field of biology and particularly relates to a beta-glucosidase, a coding gene, a recombinant carrier, engineered bacteria and application. The beta-glucosidase is derived from Thermomyces lanuginosus DSM 10635; the amino acid sequence of the beta-glucosidase is shown in SEQ ID NO: 2. The application further provides a beta-glucosidase derived from T. lanuginosus DSM 10635 and a coding gene thereof; the beta-glucosidase coding gene can be connected with an expression carrier, transformed into a Pichia pastoris strain, and a recombinant Pichia pastoris expressing the beta-glucosidase is constructed; and the beta-glucosidase with good thermal stability expressed by the recombinant Pichia pastoris is used as a catalyst to prepare ginsenoside CK through biological transformation.

The present patent application discloses a rare ginsenoside efficacy composition and a preparation method thereof. The rare ginsenoside efficacy composition of the present application comprises efficacy components such as ginseng enzymatic hydrolysate, prebiotics, dietary fiber and plant extracts, wherein the ginseng enzymatic hydrolysate is rich in rare ginsenoside CK. The rare ginsenoside efficacy composition of the present application can relieve or prevent mood disorders caused by energy restriction and elevated intestinal flora disorder, thereby improving the tolerance and long-term success rate of fat loss intervention.

This invention relates to an anti-aging composition of donkey-hide gelatin powder, its preparation method, and its application. The anti-aging composition of donkey-hide gelatin powder comprises, by mass percentage: 1-4% donkey-hide gelatin powder, 0.1-1% antioxidant and anti-inflammatory short peptides, 0.01-0.5% rare ginsenoside CK, 500-5000 ppm expression line relaxing short peptides, 1-3% solubilizer, and the balance water. By specifically setting the component content and the preparation method, a multi-level, multi-target three-dimensional anti-aging network is constructed, covering multiple key aging pathways such as nerve conduction, dermal matrix, cell metabolism, and moisturizing barrier. Furthermore, donkey oil is added to the anti-wrinkle cream used in its application, highlighting the role of donkey-hide gelatin in anti-aging in conjunction with the donkey-hide gelatin powder. The cream, serum, lotion, and lip balm formulas provided by this invention are stable, biocompatible, and possess good anti-aging and antioxidant effects, providing a safe and effective solution for addressing skin aging problems.

The invention is applicable to the technical field of biological medicines, and provides application of ginsenoside CK in preparation of a medicine for preventing or treating Alzheimer's disease. Experiments prove that the ginsenoside CK can significantly improve the spatial learning and memory ability of AD model animals and relieve anxiety behaviors of the AD model animals; the deposition of brain tissue hippocampal Abeta (1-42) protein is effectively reduced, and the key molecular pathology of AD is relieved from the source; the level of key inflammatory factors in brain tissues and serum is strongly inhibited, and AD-related chronic neuroinflammation is controlled; according to the present invention, the CK has the specific protection effect on the hippocampal neuron structure, such that the CK can be adopted as the multi-target treatment agent so as to prevent and / or treat the Alzheimer's disease.