A mutant strain Cbg61-T37, a co-expression strain Cbg61-T37-MPR1 and a construction method and application thereof

By using gene recombination and ARTP mutagenesis to screen mutant strain Cbg61-T37 and co-expression strain Cbg61-T37-MPR1, the problems of poor stability and high purification cost of β-glucosidase were solved, and the efficient and economical biosynthesis of rare ginsenoside CK was achieved.

CN121160502BActive Publication Date: 2026-06-23NORTHWEST UNIV
View PDF 8 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NORTHWEST UNIV
Filing Date
2025-09-26
Publication Date
2026-06-23

AI Technical Summary

Technical Problem

In the prior art, β-glucosidase (SS-bgly) derived from Sulfolobus solfataricus has poor stability in complex reaction systems, resulting in low synthesis efficiency of rare ginsenoside CK. In addition, the high cost of intracellular enzyme purification and recovery limits its application in large-scale biomanufacturing.

Method used

The mutant strain Cbg61-T37 was obtained through gene recombination and ARTP mutagenesis screening, and a co-expression strain Cbg61-T37-MPR1 was constructed. β-glucosidase was immobilized using cell surface display technology, and N-acetyltransferase MPR1 gene was modified to optimize fermentation conditions and improve enzyme stability and catalytic efficiency.

Benefits of technology

It significantly improves enzyme stability and catalytic efficiency, reduces production costs, and significantly increases the yield and conversion rate of rare ginsenoside CK. The catalyst has good reusability and is suitable for large-scale economic production.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN121160502B_ABST
    Figure CN121160502B_ABST
Patent Text Reader

Abstract

The application relates to the field of biotechnology, and particularly discloses a mutant strain Cbg61-T37, a co-expression strain Cbg61-T37-MPR1 and a construction method and application thereof. The mutant strain Cbg61-T37 is preserved in the China General Microbiological Culture Collection Center on August 18, 2025, has a preservation number of CGMCC No. 35649, and is classified and named as Pichia pastoris. Pichia pastoris The mutant strain Cbg61-T37 provided by the application has an enzyme hydrolysis activity of 62.9 U / mg, which is increased by 43.8% compared to the original strain. Therefore, the mutant strain Cbg61-T37 is more conducive to catalysis and synthesis of the rare ginsenoside CK.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • Beta-glycosidase mutant and coding gene thereof, and application thereof in producing ginsenoside CK

    CN103805581A

  • Gene engineering bacteria capable of resisting reactive oxygen species (ROS) oxidative stress and efficiently secreting express foreign protein and construction method of gene engineering bacteria

    CN103981111A

  • Method for screening bacterial strains with ability of converting massively existing ginsenoside into rare ginsenoside

    CN104232671A

  • Production of non-caloric sweeteners using engineered whole-cell catalysts

    CN107548417A

  • Beta-glycosidase SS-BGL mutant for modifying ginsenoside and application of beta-glycosidase SS-BGL mutant

    CN116590259A