150 results about "Ganoderic acid" patented technology

A method for using ganoderma lucidum liquid fermentation liquor to prepare feed and feed additive containing ganoderma lucidum active ingredient, pertains to the field of biofermentation and feed. The invention includes steps of: a. centrifugally separating the ganoderma lucidum liquid fermentation liquor at 5000 per-minute to obtain the ganoderma lucidum fermentation supernatant and ganoderma lucidum residues; b. adopting a rear spraying process to mixing the ganoderma lucidum fermentation supernatant with the feed in a proportionality of adding 0.5-1.5 ml of the supernatant in the feed for preparing feed containing ganoderma lucidum active ingredient; c. performing a second fermentation to the ganoderma lucidum residues for preparing the feed additive containing ganoderma lucidum active ingredient. The invention fully use the ganoderma lucidum fermentation liquor, has no waste water and waste slag discharge, simple steps, low cost, and easy large-scale industrialization. Products are rich in Ganoderma lucidum polysaccharides and ganoderma lucidum acid; have a balance immune system, and antibacterial, antiviral, anti-parasitic, anti-tumor, anti-radiation and the like extensive physiology activities, thereby largely improving the immune disease resistance, maintaining the animal intestinal health, and further improving the production performance and market efficiency of animals.

The invention aims to disclose high-bioactivity ganoderma spore oil and a preparation method thereof, and belongs to the field of Chinese medicament. The method comprises the following steps: breaking walls of ganoderma spore powder by adopting a high-energy nano impact mill, performing dry granulation, supercritical CO2 extraction, molecular distillation and purification, and thus obtaining the ganoderma spore oil. The operation process of the method is carried out in an anhydrous environment, so that oxidation of the spore oil in the processing process is reduced, and the obtained product is stable in property; the freezing transparent test time of the ganoderma spore oil prepared by the method exceeds 5.5 hours; and the measured unsaturated fatty acid content is between 60 and 80 percent, the total triterpenoid content is 2 to 3 percent, the total ergosterol content is 2 to 5 mg/g, the ganoderic acid A content is more than 1 mg/g, the peroxide value is less than 5mmol/kg, and the acid value is less than 10 mg KOH/g.

The present invention discloses one kind of compound glossy ganoderma spore oil and its preparation process. Compound glossy ganoderma spore powder as material is dry pressed to palletize and extracted in a extracting kettle with supercritical CO2 or ultrasonically to obtain pale yellow compound glossy ganoderma spore oil. The compound glossy ganoderma spore oil contains total organic acid over 90 % and total ganoderic acid in 0.1-2.5 %, and has acid value below 25. The process of the present invention is simple, convenient and suitable for industrial production, and the prepared compound glossy ganoderma spore oil is used in medicine and food.

The invention relates to an aqueous two-phase system extraction method for ganodenic acid and ganoderma lucidum polysaccharide. The method particularly comprises the following steps of: (1) pulverizing fresh or dry ganoderma lucidum fruiting bodies; (2) establishing an ethanol/ammonium sulfate aqueous two-phase system; (3) performing extraction separation: adding ganoderma lucidum powder into the ethanol/ammonium sulfate aqueous two-phase system, shaking up the mixture by vibration or uniformly mixing the mixture by ultrasonic sound, and standing the mixture for phase splitting or centrifugally splitting the phase to obtain a top phase and a bottom phase; (4) concentrating the top phase under reduced pressure and freeze-drying the top phase to obtain the ganodenic acid; and (5) dialyzing the bottom phase to obtain permeate and intercept fluid, performing alcohol precipitation on the intercept fluid with 80 percent ethanol and centrifuging to obtain a precipitate and supernatant, and free-drying the precipitate to obtain the ganoderma lucidum polysaccharide. The method has the advantages of simple process, low equipment requirement, low cost, no pollution, suitability for batch production and capability of realizing synchronous extraction of the ganodenic acid and the ganoderma lucidum polysaccharide.

The present invention is one kind of glossy ganoderma glycopeptide and its preparation process. The glossy ganoderma glycopeptides consists of wall breaking glossy ganoderma spore powder 5-15 %, glossy ganoderma spore oil 10-30 %, glossy ganoderma extract 15-40 %, and plant glycopeptide polymer for the rest. The preparation process includes the following steps: cultivating glossy ganoderma sporophore, enzymolysis biologically and crushing to break wall, alcohol reflux extracting to obtain ganoderic acid, polysaccharide and peptides, adsorption with resin and freeze drying, mixing, pelletizing and packing. The glossy ganoderma glycopeptides contains over 100 kinds of beneficial components, and has absorption rate up to 98 % and fast health effect.

The invention provides a preparation method of cultivating substitute of lucid ganoderma, and relates to cultivating substitute of edible mushrooms. The cultivating substitute is prepared by the components which are mixture of pine, cedar, rotten wood, miscanthus sinensis, brans and bean dregs. The preparation method comprises the following steps: crushing the mixture of rotten wood, brans and bean dregs and the miscanthus sinensis, respectively; weighing the crushed materials in mass percent as requirement, and uniformly mixing; uniformly mixing the uniformly mixed material and water, and then fermenting; adding processed sawdust or sawing chips of pine and cedar to uniformly mix, and then uniformly mixing the mixture and water; and finally bagging and sterilizing the uniformly mixed mixture, thus obtaining the cultivating substitute of lucid ganoderma. According to the technical scheme mentioned above, the required raw materials for preparing the cultivating substitute are easy to obtain, and low in cost; and moreover, the lucid ganoderma cultivated by the cultivating substitute is fragrant, bitter in taste, and has high content of triterpenoids ganoderic acid, as well as high value in use.

The invention relates to the industrial field of biological feed, in particular to the method of feed containing ganodenic acid produced by converting medicinal ganodermalucidum into cornstock, and the effect of the functional feed produced through the method. In the invention, liquid submerged culture, seeding tank extended culture and solid-state fermentation culture are conducted with the ganodermalucidum as the starting bacterial strain and the cornstock as the auxiliary material, to obtain the functional feed rich in ganodenic acid. The feed of the invention is light yellow powder and has fragrance; the content of crude ganodenic acid in the feed is up to 0.06 percent to 0.3 percent, and the content of protein is 12 percent to 14 percent. The feed containing ganodenic acid can be used as the additive of animal feed and for preparing medicine or health care food which have the functions of regulating blood sugar and lipid, and preventing cardio-cerebrovascular diseases or bronchitis. The invention not only develops a new approach for deeply developing and utilizing the ganodermalucidum, but also provides ideal biological additive for developing the functional feed.

The invention provides a preparation technology of sporoderm-broken spore powder of aselenium enrichment imitation wild ganoderma lucidums. The technology comprises the following main processes of planting selenium enrichment imitation wild ganoderma lucidums, collecting the ganoderma lucidum spore powder by perforating and covering the whole membranes, sinking, floating, and removing impurity circularly, impacting the sporoderm-broken spore powder by adopting variable temperature embrittlement nanotechnology, purifying concentrated sporoderm-broken spore powder through multilayer macromolecule separating membranes. The preparation technology of the sporoderm-broken spore powder of selenium enrichment imitation wild ganoderma lucidums has the advantages that the content of organic selenium in the poroderm-broken spore powder is increased, anti-cancer and detoxification effects of ganoderma lucidum spores are improved, the immunity is boosted, the effect of enhancing metabolism is facilitated, contents of effective components such as ganoderma lucidum polysaccharide, ganoderic acid and amino acid in the ganoderma lucidums are obviously increased, the productive rate of the spore powder is increased by 35%, the effective components in the sporoderm-broken spore powder are free from loss, the preparation technology is simple and convenient to operate, high in speed, low in cost and the content of effective components in the sporoderm-broken spore powder products is increased by 50% above.

The invention provides a preparation method for a functional Ganoderma lucidum fermented feed. The preparation method provided by the invention is based on high-quality utilization of regeneration resources; raw materials for the Ganoderma lucidum fermented feed include a Ganoderma lucidum seed nutrient solution and a solid-state fermentation medium; and through reasonable matching of a variety of raw materials and usage of Ganoderma lucidum biofermentation and conversion technology, the Ganoderma lucidum fermented feed with a plurality of functions are prepared. The prepared Ganoderma lucidum fermented feed contains functional components like Ganoderma lucidum polysaccharide, chito-oligosaccharide, ganoderic acid, enzyme-digested small peptide and a compound enzyme. The preparation method provided by the invention overcomes influence of anti-nutritional factors in cereal materials of the feed on the utilization rate of the feed and improves the utilization rate of the feed. Experimental results prove that the Ganoderma lucidum fermented feed prepared in the invention is capable of preventing and resisting diseases and regulating intestinal floras.

The invention provides a quality evaluation method for a ganoderma lucidum medicinal material. The quality evaluation method comprises the following steps: taking a ganoderma lucidum extract comparison product as comparison, and measuring a ganoderma lucidum medicinal material sample to be measured by liquid chromatography at any wavelength in a detecting wavelength range of 210 to 280 nm; summing all ultraviolet absorption peak areas of which the retention time is in the interval of 15 to 65 minutes in an obtained liquid chromatogram, and comparing the sum with an ultraviolet absorption peak area corresponding to ganoderic acid A in the comparison product; calculating the content of total ganoderma triterpenes in the ganoderma lucidum medicinal material sample to be measured; taking the content of the total ganoderma triterpenes as a quality index for evaluating the quality of the ganoderma lucidum medicinal material. According to the quality evaluation method for the ganoderma lucidum medicinal material provided by the invention, the quality of the ganoderma lucidum can be evaluated more accurately, comprehensively and conveniently, and the detection cost can also be reduced.

The invention discloses a method for heterologous biosynthesis of ganoderic acid through synthetic biological means. The method comprises the following steps: exploring cytochrome P450 enzyme (CYP) gene related to bio-synthesis of ganoderma triterpenes, and promoting heterologous expression of the gene in saccharomyces cerevisiae cells so as to achieve screening and identifying; and separating andpurifying a fermentation product of a yeast engineering strain, and conducting such analysis as mass spectrum, nuclear magnetic resonance and the like, so as to confirm 3-hydroxy-lanosta-8 and 24-dien-26-oci-acid (ganoderic acid Z). The method provided by the invention comprises a series of design and verification of exploring the bio-synthesis catalysis element (the gene) from the ganoderma triterpenes, fermentation phenotype priliminary screening and element function identifying, and finally, obtaining ganoderma triterpene substances of heterologous biosynthesis and the like, and the methodalso offers an example for heterologous production of other ganoderma triterpene active substances.

The invention discloses a high-yield engineering strain kmust-SQS for a ganoderic acid. The preservation number of the high-yield engineering strain kmust-SQS in the China General Microbiological Culture Collection Center (CGMCC) of the China Committee for Culture Collection of Microorganisms (CCCCM) is CGMCC NO.9059. The ganoderic high-yield engineering strain kmust-SQS is obtained through the overexpression coding of squalenesynthase (SQS) via the selection of a strong ganoderic promoter P-gpd. The shake flask fermentation experiment shows that compared with the content of the ganoderic acid in a wild-type strain (WT strain), according to the high-yield engineering strain kmust-SQS, the content of the ganoderic acid in an SQS converter strain is improved by 23.8% under the condition that the cell growth of the high-yield engineering strain kmust-SQS is not influenced, wherein the contents of ganoderic acid monomers GA-Me, GA-T, GA-Mk and GA-S in the SQS converter strain are respectively improved by 82.96%, 49.84%, 174.51% and 31.80% as compared with the WT strain. Thus, the high-yield strain kmust-SQS can serve as an engineering strain used for producing the ganoderic acid. As a result, the high-yield strain kmust-SQS has a wide application prospect.

The invention discloses a ganoderic acid high-producing engineering strain kmust-VGB-1, which is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC NO. 11301. The lucid ganoderma engineering strain kmust-VGB-1 for high production of ganoderic acid is prepared by expressing a vitreoscilla hemoglobin VGB gene through a lucid ganoderma strong promoter P-gpd; through a fermentation experiment in a shaking flask, under the circumstance of avoiding influence on cell growth, yield of monomer ganoderic acids GA-Me, GA-T, GA-Mk and GA-S produced by the VGB converter strain are respectively 3.1 times, 3.2 times, 2.1 times and 3.6 times that of monomer ganoderic acids produced by a WT strain; therefore, the high-producing strain, as an engineering strain for producing the ganoderic acid, has a broad application prospect.

The invention relates to a monomeric 7-ethyoxyl ganoderic acid O and a separating and purifying method of a monomeric 7-ethyoxyl ganoderic acid T thereof, belonging to the technical field of extraction of organic matters. The separating and purifying method comprises the following steps: preparing dried thallus extract, screening components containing 7-ethyoxyl ganoderic acid O strips, carrying out crystallization treatment, and preparing extract crystal; using reverse liquid-phase chromatogram to analyze crystal purity of ganoderic acid crystal; and using methanol solution to dissolve the ganoderic acid crystal, adding hydrochloric acid in the methanol solution, then placing the obtained solution in an acidolysis reactor for acidolysis treatment to obtain monomeric ganoderic acid crude solution, and finally crystallizing after rotary evaporation treatment and obtaining the ganoderic acid T. The invention can directly obtain ganoderic acid T monomer with the purity of more than 98 percent or 7-ethyoxyl ganoderic acid O with the purity of more than 95 percent, has easy operation and convenient promotion and can lays solid foundation for development and production of relevant drugsof the monomeric ganoderic acid in future.

The invention discloses a method for high-yield and high-quality culture of lucid ganoderma with application of light and relates to the technical field of medical fungi culture. According to the invention, control with light of different wavelengths is applied during the whole culture course of lucid ganoderma, and LED lamps with different colors are used as light sources; or, different light qualities are obtained through placement of a filter coating on a culture object under a common light source or natural light. Through regulation and control of different light qualities, growth and development states of lucid ganoderma hyphae could be changed. On one side, growth speed of lucid ganoderma is increased. On the other side, the contents of effective constituents in lucid ganoderma (ganoderan and ganodenic acid) are increased. Hence, the growth cycle of lucid ganoderma is increased, and lucid ganoderma quality is improved. According to the invention, the method is simple; the cost is low; the lucid ganoderma cultured by the method has higher commodity values; and significant economic benefits can be obtained.

The measuring method measures contents of general ganoderma lucidum acid, ganoderma lucidum acid E in spore oil preparation of ganoderma lucidum. The testing standard is as following: in each 1.0g of spore oil preparation of ganoderma lucidum (equivalent to 5g of crude drugs of ganoderma lucidum spore), general ganoderma lucidum acid contained is not allowed less than 1.0mg counted by ganoderma lucidum acid E (C30H54O7); content of ganoderma lucidum acid E is between 0.001mg-0.050mg, i.e. content of ganoderma lucidum acid E is between 0.010%-0.50%. The invention makes stable testing and controlling quality of spore oil preparation of ganoderma lucidum.

The invention discloses an application of ganoderic acid A to preparation of drugs for activating brain CD4+T cells and inhibiting neuroinflammation. Experiments show that the ganoderic acid A significantly up-regulates the content of CD4+T in mouse brains, reduces the levels of L-1beta, IL-6, TNF-alpha, NF-kB and the like, and down-regulates expression of Abeta1-42, p-Tau, APOE, IBA-1, and GFAP,which suggests that the ganoderic acid A can activate the CD4+T cells in brains and inhibit neuroinflammation. Acute toxicity experiments on cells and animals show that the safe dose range of the ganoderic acid A is wide, and can be applied to preparation of the drugs for activating the CD4+T cells in brains and inhibiting neuroinflammation.

The invention discloses a method for utilizing ganoderma lucidum to convert grape skin to produce a functional food and relates to the technical field of bioengineering. According to the preparation method, sequentially through the steps of enlarged culture in a test tube, liquid shake-flask culture and enlarged culture in a seed tank, solid fermental cultivation, drying and smashing, the functional food which regulates blood lipids, blood sugar and blood pressure, resists radiation and inhibits tumor is obtained. According to the method, the grape skin, rice, wheat, corn and sorghum and the like are taken as solid substrates, ganoderma lucidum is taken as a strain to convert a grape skin flavone, and ganodenic acid and ganoderma lucidum polysaccharide; in the obtained product, the content of the flavone is 2-50 mg/g of dry substrate, the content of the ganoderma lucidum polysaccharide is 10-100 mg/g of dry substrate, the content of ganodenic acid is 1-20 mg/g of dry substrate, and the method can be used for extracting active ingredients flavones, ganoderma lucidum polysaccharide and ganodenic acid of ganoderma lucidum and producing tablets or capsules for treating the blood lipids, blood sugar and blood pressure, resisting radiation and inhibiting tumor.

The invention relates to a novel purpose of ganoderic acid, in particular to application of the ganoderic acid G in various tumour treatments. The ganoderic acid G disclosed by the invention comprises various preparations including solid type preparations of troches, capsules and the like, ointment, pasting agents, injections and the like, which can be prepared by adopting a conventional method in a pharmaceutical field. The tumour treatment medicine provided by the invention, namely the ganoderic acid G, has the function of directly inhibiting tumour cell proliferation under small dose; at this time, the ganoderic acid G has low toxic effect on normal tissue cells; and the effect of further treating tumours is realized after adding super-antigen. The application of the ganoderic acid G as the immune synergist and the super-antigen dependent therapeutic medicine in the tumour treatments disclosed by the invention has the obvious advantages and the technical progresses that: when the ganoderic acid G is united with the super-antigen, the function of killing and wounding a wide variety of tumour cells in extracorporeal and esoteric experiments can be effectively played.

The invention provides the uses of ganoderic acid T and ganoderic acid me in preparation of agents for inhibiting the growth and proliferation of tumor cells. In vitro and in vivo studies have shown that ganoderic acid T and ganoderic acid Me have significant effect of inhibiting the growth and proliferation of human tumor cells while have less toxicity on normal cells. It has good application prospect.

Use of a pharmacodynamic active ingredient of ganoderma lucidum in preparing anti-depressant drugs, and anti-depressant drugs comprising the pharmacodynamic active ingredient of ganoderma lucidum, the pharmacodynamic active ingredient being ganoderic acid A or a derivative of ganoderic acid A, the derivatives being glycoside compounds of ganoderic acid A with pharmaceutically acceptable salts, esters or sugar thereof.