25 results about "Ganoderic acid" patented technology

The invention discloses application of a gene GlZn2Cys661 in regulation and control of synthesis of ganoderic acid. The nucleotide sequence of the gene GlZn2Cys661 is as shown in SEQ ID NO. 1. The gene expression is regulated and controlled by constructing an overexpression or silent vector, so that the synthesis of the ganoderic acid is promoted or inhibited, and an effective method is provided for regulating and controlling the yield of the ganoderic acid. The invention relates to the technical field of bioengineering, and discloses an application of a gene GlZn2Cys661 in regulation and control of ganoderic acid synthesis. The invention provides a key gene target and a core engineering strain for efficiently producing ganoderic acid through a metabolic engineering means, and has important application value.

The invention discloses ganoderic acid mass production and application based on cytochrome P450 enzyme CYP512W6, the cytochrome P450 enzyme CYP512W6 in ganoderma lucidum is converted into a corresponding saccharomyces cerevisiae strain for heterologous expression, and three kinds of ganoderic acid, namely TLTOA, 3 beta-TLTOA and DLDOA, are obtained after fermentation. The invention provides a new implementation mode for heterologous biosynthesis of ganoderic acid in saccharomyces cerevisiae.

The invention discloses a culture medium composition for culturing dendritic cells and a culture method, and belongs to the field of biological medicines. Aiming at the problems of long induced differentiation period and low purity of the existing DC, the invention provides the culture medium composition for culturing the dendritic cells, the culture medium composition comprises a basic culture medium, an induction culture medium, a maturation culture medium and a function strengthening culture medium, the induction culture medium comprises the basic culture medium, and the maturation culture medium comprises a mature culture medium and a function strengthening culture medium; the GM-CSF, the IL-4, the astragalus polysaccharide and the rapamycin are added into the basic culture medium; the mature culture medium comprises a basic culture medium, and TNF-alpha, IFN-gamma, ganoderic acid and Poly (I: C) which are added into the basic culture medium, the function-enhanced culture medium comprises a basic culture medium, and IL-10, IL-12 and vitamin D3 which are added into the basic culture medium. The culture medium in each stage is combined with the synergistic effect of specific cell factors and natural plant extracts, so that high-purity and high-activity induction of the DC is realized, and the DC has the capability of resisting tumor microenvironment inhibition.

The invention relates to a method for heterologously producing ganoderic acid, which comprises the following steps: respectively transforming candidate oxidase, candidate reductase and candidate acetyltransferase into corresponding saccharomyces cerevisiae strains for heterologously expressing, and screening the transformed strains through intracellular fermentation or whole-cell catalysis to obtain codon-optimized AfuSDR and CsSDR capable of catalyzing oxidization of hydroxyl of a ganoderma triterpene skeleton C3, the method comprises the following steps of: performing independent expression or combined expression on reductases AKR1C2, AKR1C4 and FusC1 which are optimized by codons and are used for catalyzing C3 keto reduction and acetyltransferase BsAT which is optimized by codons and is used for catalyzing C3 hydroxyl acetylation, so as to realize heterologous biosynthesis of ganoderic acid TR (GA-TR), gibberellic acid Ja (GA-Ja) and ganoderic acid Mf (GA-Mf).

The invention discloses synthesis and application of a ganoderic acid molecularly imprinted magnetic microporous organic network material for selectively enriching ganoderic acid substances, and belongs to the field of material preparation. The ganoderic acid molecularly imprinted magnetic microporous organic network material with large specific surface area, good selectivity and high adsorption capacity is prepared by a simple one-pot method. The material contains rich delocalized pi bonds, carboxyl groups, amino groups and specific hydrogen bond interaction sites, and can generate relatively strong hydrogen bond interaction and pi-pi interaction with ganoderic acid substances containing active sites such as steroidal rings, hydroxyl groups and carbonyl groups, so that an enhanced enrichment effect is achieved. The material is used as an adsorbent of a magnetic solid-phase extraction method and is used for high-selectivity enrichment and high-sensitivity detection of ganoderic acid substances.

This invention relates to the technical field of detecting active ingredients in Ganoderma lucidum and shiitake mushroom oral liquid, and provides a method for simultaneously detecting ergothioneine and ganoderic acid A in Ganoderma lucidum and shiitake mushroom oral liquid, comprising the following steps: detecting Ganoderma lucidum and shiitake mushroom oral liquid using high performance liquid chromatography (HPLC); the HPLC column used is a JADE-PAK NH2 column, the column temperature is 30-40℃, the detector is an ultraviolet detector, and the detection wavelength is 252-256nm; the mobile phase includes mobile phase A and mobile phase B, mobile phase A is an acetonitrile solution, mobile phase B is a 0.1% (V / V) aqueous solution of acetic acid, the volume ratio of mobile phase A to mobile phase B is (74-76):(24-26), and the flow rate of the mobile phase is 0.9-1.1 mL·min. ‑1 This invention utilizes a JADE-PAK NH2 amino column for the detection of Ganoderma lucidum and shiitake mushroom oral liquid, and through optimization of chromatographic conditions, enables accurate and rapid simultaneous determination of the contents of ganoderic acid A and ergothioneine in Ganoderma lucidum and shiitake mushroom oral liquid.

The invention discloses a novel oil-soluble micelle preparation. The component A comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, cardiolipin, cephalin and dipalmitoyl phosphatidylcholine, and the component B comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, phosphatidylcholine, cephalin and dipalmitoyl phosphatidylcholine; one or more of dipalmitoyl phosphatidyl ethanolamine and dipalmitoyl phosphatidyl ethanolamine; the component B comprises a component B1 and a component B2, and the component B1 comprises one or more of phytosterol, cholesterol, ergosterol, protopanoxadiol, protopanaxatriol, ganoderic acid, phytol, isophytol, retinol, andrographolide, paclitaxel, artemisinin and bisabolol; and the component B2 is prepared from one or more of 1, 2-hexanediol, ethylhexylglycerin, cyclohexyl glycerin, absolute ethyl alcohol and isosorbide dimethyl ether. The key point of the invention is to provide a low-cost formula and a low-cost preparation method for preparing the oil-soluble micelle preparation.

The present application relates to a bacillus velezensis strain JZ01, a microbial agent containing the same and application thereof. The bacillus velezensis strain JZ01 can efficiently antagonize the pathogen of cucumber brown spot and effectively kill root-knot nematodes. Further, the present application also develops a synergist of the strain. It is found that trigonelline can be used as a synergist to significantly increase the efficacy of the strain against the pathogen of cucumber brown spot, and ganoderic acid A can enhance the efficacy of the strain in killing root-knot nematodes, so that a more efficient compound microbial agent can be developed. In addition, the bacillus velezensis strain and the microbial agent containing the same are microbial agents, which are safe and environmentally friendly, and therefore have high industrial value and application prospect.

The application provides preparation and application of a PROTAC degrading agent, and relates to the field of medicines.The ganoderic acid A-PROTAC compound with degrading activity provided by the application takes ganoderic acid A as raw material, retains the structural skeleton of ganoderic acid A, and is connected with E3 ubiquitin ligase Cereblon (CRBN) or VHL ligand by using different lengths of fatty chains or polyethylene glycol (PEG) chains to obtain ganoderic acid A derivatives GAA C1-C10 and V1-V10.The ganoderic acid A derivative with novel antitumor effect and the like is synthesized by a simple synthesis method.The ganoderic acid A derivative has the structure shown in the following general formula I.

The invention relates to the technical field of food and beverage development, in particular to a cherry sleep-aiding beverage and a preparation method thereof. The natural components of the lucid ganoderma, the spina date seeds and the lavandula angustifolia, the aminobutyric acid and the theanine are added, so that a basis is mainly provided for the sleep-aiding function of the cherry sleep-aiding drink; meanwhile, medium-chain triglyceride, thiamine hydrochloride, propylene glycol alginate, lysine and magnesium carbonate are introduced, so that the lucid ganoderma, the spina date seeds, the lavandula angustifolia, the aminobutyric acid and the theanine stably exist in the cherry sleep-aiding beverage, and the possible chemical reaction process with other components in the storage process is avoided; the function weakening and even disappearance caused by interference of other components can be avoided, and the sleep aiding function can be enhanced; the cherry concentrated solution, the sodium citrate, the vitamin C and the pectin are introduced, so that a cherry flavor and a stable beverage system are obtained, and the uniformity and the stability of each component are ensured.

The invention provides a composition and application thereof in preparation of a reagent for resisting skin aging. The composition is prepared from afzelin and ganoderic acid A. The method can solve the problem that in the prior art, methods and products for resisting skin aging have limitations, and is suitable for the field of skin aging resistance.

This utility model discloses an extraction and purification device for ganoderic acid, belonging to the field of medicinal material processing technology. It solves the problem that unmelted ganoderic acid particles easily accumulate on the surface of the filter membrane of existing devices, and the surface of the particles easily adsorbs ganoderic acid solution, leading to waste of ganoderic acid solution. The device includes a filter box with a cover fixedly installed on the top surface and a connecting pipe fixedly connected to the bottom surface of the filter box. The connecting pipe is connected to a preparative chromatography device. The filter box contains a filter assembly, which includes a motor, a filter cylinder, and a liquid inlet pipe. The filter cylinder is hollow inside and has a liquid inlet hole in the middle of the right end face. Ganoderic acid solution is added to the inside of the filter cylinder through the liquid inlet pipe. The motor drives the filter cylinder to rotate, which can throw out the ganoderic acid solution inside the filter cylinder and adhering to the ganoderic acid particles, so as to fully filter out the ganoderic acid solution and avoid waste of ganoderic acid solution.

This invention discloses a method for preparing Ganoderma lucidum and jujube seed compound freeze-dried flash release tablets by enzymatic hydrolysis and fermentation, which relates to the field of traditional Chinese medicine preparation technology. The method includes the following steps: (1) Raw material pretreatment: Jujube seed is crushed and mixed with citrate buffer to obtain jujube seed slurry; Ganoderma lucidum fruiting body is ultra-finely pulverized and mixed with cut lily petals to obtain mixed raw materials. This invention uses a specific ratio of compound enzymes (cellulase, pectinase, β-glucanase) in an optimized acidic environment for enzymatic hydrolysis, which can efficiently and selectively destroy the plant cell walls of jujube seed, Ganoderma lucidum and lily, and fully release the active ingredients in the cells; the subsequent fermentation of Lactobacillus plantarum can further decompose the substrate and may produce new beneficial metabolites, forming a synergistic biotransformation, which greatly improves the extraction rate of total active ingredients and product value.

This application relates to the technical field of natural product compositions, specifically an anti-glycation composition. This application discloses a composition system formulated from basilin C, an active ingredient from the medicinal and edible herb Gastrodia elata, and ganoderic acid C, an active ingredient from Ganoderma lucidum, which can reduce the accumulation of advanced glycation end products (AGEs). In vitro anti-glycation activity screening results show that this composition has inhibitory activity against protein glycation, and the inhibitory activity is significantly higher than that of single-component natural products. Furthermore, this composition has no significant cytotoxicity, indicating that this composition system can be used as an anti-glycation ingredient in the development of pharmaceutical technology, and can also be used to manufacture pharmaceuticals, food, cosmetic products, and skincare products.

The application belongs to the technical field of disease drug treatment, and particularly relates to application of a CLK2 inhibitor in preparation of a drug for treating IgA nephropathy. The CLK2 inhibitor (such as ganoderic acid A methyl ester) can significantly reduce 24hUTP, 24hmAlb, BUN and Scr levels of an IgA nephropathy model, and IGA deposition in the inside of a glomerulus, so as to achieve the purpose of effectively treating IgA nephropathy.

The invention discloses a method for simultaneously determining nine index components in a Zhibeng bacteriostatic liquid and constructing a characteristic chromatogram and application, and belongs to the technical field of detection and analysis. Octadecylsilane chemically bonded silica is used as a filling agent; 0.1% phosphoric acid water is used as a mobile phase A, and acetonitrile is used as a mobile phase B; the detection wavelength 316 nm is used for establishing a characteristic spectrum and determining the content of cimicifugin and liquiritin, the detection wavelength 402 nm is used for determining the content of hydroxysafflor pigment A and dehydrated safflower yellow B, the detection wavelength 254 nm is used for determining the content of ganoderic acid A and schizandrin, and the detection wavelength 275 nm is used for determining the content of lozastilbin, salidroside and 5-hydroxymethylfurfural. According to the method, separation and accurate detection are realized by adopting a gradient elution procedure. So far, no report about simultaneous determination of the nine components is found, and the method makes up the blank of quality control and fingerprint spectrum research in prescriptions containing the medicinal materials.

The invention provides a method for simultaneously detecting six types of I-type ganoderic acid in ganoderma lucidum fermented mycelia. The method comprises the following steps: (1) pretreating the ganoderma lucidum fermented mycelia; (2) an ultra-high performance liquid chromatography detection step; (3) obtaining mass spectrum information of the compound; (4) establishing a dynamic multi-reaction monitoring DMRM analysis method; and (5) a data analysis step. According to the method for simultaneously detecting the six types of I-type ganoderic acid in the ganoderma lucidum fermented mycelium, accurate qualitative and quantitative analysis can be simultaneously carried out on the six types of I-type ganoderic acid compounds, so that an accurate and reliable detection means is provided for related basic research and product development and application of I-type triterpenes in ganoderma lucidum.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a fermentation preparation method and application of ganoderma lucidum with full-effect components. The method comprises the following steps: sequentially carrying out strain pretreatment, two-stage fermentation regulation, graded extraction, eutecticum construction and composite preparation: treating a ganoderma lucidum strain by adopting a domestication culture medium containing corn steep liquor and Tween-80 and an activation culture medium containing beta-cyclodextrin; two-stage fermentation is formed through aerobic proliferation and anaerobic induction, and methyl jasmonate is added in the anaerobic stage and is irradiated by blue light; carrying out supercritical COextraction on fat-soluble components, carrying out water extraction on water-soluble components, and carrying out ultrafiltration and enrichment on small molecule peptides to finish graded extraction; the ganoderic acid DM and the lycium barbarum polysaccharide are prepared into eutecticum, and then the eutecticum is compounded with the water-soluble component and the small molecule peptide in proportion. The strain adaptability and mycelium biomass can be improved, the ratio of ganoderic acid DM is directionally increased, and the solubility and absorption efficiency of fat-soluble components are improved.

The invention discloses a step-by-step heat stress cultivation method for promoting production of ganoderic acid, which comprises the following steps of: culturing a lucid ganoderma strain under an optimized condition to increase the biomass of lucid ganoderma, and then performing HS treatment by optimizing a variable temperature regulation mode, namely adopting a single-time or intermittent heat stress regulation treatment mode in the HS treatment process to improve the yield of ganoderic acid. Therefore, a good effect of promoting the production of total ganoderic acid and specific ganoderic acid is achieved. The method has the advantages that the contradiction and difficulty that the total amount of GA is reduced due to the fact that the general heating heat stress treatment is not beneficial to biomass accumulation are overcome, and a specific step-by-step culture and gradient heat stress treatment mode is adopted; and meanwhile, according to the characteristics of different growth stages, the synergistic effect of conditions such as proper illumination and additives is supplemented to optimize the process of culturing the ganoderma lucidum strain and promoting the GA production, so that a specific production effect is achieved in a short time.

1. Name of the product in this design: Packaging Kit (Ganoderma Lucidum and Jujube Seed Herbal Drink). 2. Purpose of this design: for product packaging. 3. The key design feature of this product is its pattern. 4. The image or photograph that best illustrates the design highlights: the main view of Suite 3. 5. This product is a thin product, and the left view, right view, top view, and bottom view of kit 1 are omitted. 6. Other matters requiring explanation: This design product is a kit product, therefore it is used and sold as a set. Kit 1 is the inner packaging bag, kit 2 is the inner packaging box, and kit 3 is the outer packaging box. Kit 1 is placed inside kit 2 and then inside kit 3 for sale and use.

The invention relates to an active ganoderic acid yield optimization method based on directed evolution cytochrome P450, which is characterized in that on the basis of a prediction structure of AlphaFold2 aiming at a wild type CYP512W2 gene, key mutant screening is carried out based on conformation analysis of CYP512W2 and a substrate, and iterative mutant screening is carried out through molecular dynamics simulation and structural analysis, so that the prediction precision and efficiency of enzyme modification are improved; therefore, the yield of the ganoderma triterpenoids is effectively improved. According to the invention, a combined strategy of rational design based on structural analysis and accelerated enzyme modification is adopted to optimize and screen mutants and iterate mutants, so that a strain capable of improving the yield and selectivity of type II ganoderic acid Y and Jb produced under the catalysis of membrane protein P450 is obtained.

The invention belongs to the technical field of food deep processing, and provides a method for solid-state fermentation of tartary buckwheat with high yield of triterpenoid active components by using morchella. The preparation method comprises the following steps: inoculating a morchella fermentation liquid into sterilized tartary buckwheat, carrying out constant-temperature fermentation culture at 24-30 DEG C for 15-25 days, and drying at 60 DEG C for 18 hours, so as to obtain the morchella solid-state fermented tartary buckwheat with high yield of triterpenoid active ingredients, the sterilized tartary buckwheat is prepared by the following steps: soaking 50g of tartary buckwheat in purified water with the volume 4 times that of the tartary buckwheat for 24 hours, draining, and sterilizing for 1 hour by high-pressure steam at 121 DEG C; the inoculum size of the morchella fermentation liquor is 3-7 mL. The four triterpenoid components are analyzed by using non-targeted metabonomics, the four triterpenoid components are all in an up-regulation trend in a tartary buckwheat solid-state fermentation process by using morchella, the up-regulation trend of beta-citraulin and ganoderic acid is most obvious, and relative quantitative values are respectively up-regulated by 404.44% and 195.42% when the tartary buckwheat is fermented for 20 days; in addition, the two triterpenoid components are important intermediates in the fungal cell proliferation process, and up-regulation of response values of the two triterpenoid components also reflects continuous proliferation of fungal cells in the fermentation process.

This invention discloses a highly efficient, large-scale method for preparing ganoderic acids, comprising the following steps: pulverizing fresh Ganoderma lucidum (12-20 days old), subjecting it to dynamic cold water extraction at 20-35℃, two-stage centrifugation, microfiltration and ultrafiltration for impurity removal, followed by vacuum concentration; the concentrated solution is then purified by two-stage chromatography using a polyamide resin column and an AB-8 or D101 macroporous adsorption resin column, and the target eluent is collected and dried. This invention abandons high-temperature and large-volume organic solvent extraction, employing low-temperature water extraction combined with dual-column coupling technology. Utilizing the complementary adsorption characteristics of polyamide and macroporous resin, polysaccharides, proteins, and structural analogs are effectively removed; the resulting ganoderic acid product has a total purity ≥90% and retains complete biological activity.

The application provides application of ganoderic acid A in preparation of anxiolytic drugs and belongs to the technical field of medicines. The application first proposes that ganoderic acid A has an anxiolytic effect. It is verified through animal experiments that ganoderic acid A with a dose of 0.01 mg / kg-20 mg / kg can effectively improve anxiety of mice. The application further provides an anxiolytic drug. The active ingredient of the drug comprises ganoderic acid A, and the drug can effectively resist anxiety.

1. The name of the design product: tea packaging bag (Yuan Yuan tea - Ganoderma acid jujube lily tea). 2. The use of the design product: for packaging tea. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: front view. 5. The design product is a thin product, and the left view, right view, top view and bottom view are omitted.