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Method for utilizing ganoderma lucidum to convert grape skin to produce functional food

A technology for functional food and grape skin, applied in the field of bioengineering, can solve the problem that it is difficult to make full use of the functional components of grape skin, and achieve the effect of realizing industrialized large-scale production, enhancing the amount, and overcoming the inhibitory effect.

Inactive Publication Date: 2016-11-23
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few reports on the use of medicinal edible fungi to carry out biotransformation research on grape skins. At the same time, it is difficult to fully utilize all the functional components of grape skins by simple extraction. Therefore, it is particularly necessary to develop a method for fully utilizing all functional components of grape skins.

Method used

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  • Method for utilizing ganoderma lucidum to convert grape skin to produce functional food

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Such as figure 1 As shown, a method for utilizing Ganoderma lucidum to transform grape skin to produce functional food specifically includes the following steps:

[0051] (1) Test tube expansion culture: Cut the slant strain of Ganoderma sinense CGMCC 5.69 test tube into small pieces of 3×3 mm, inoculate a small piece into the slant medium of the test tube, and cultivate it at 20°C for 15 days to prepare the slant of the test tube Bacterial strains, the inclined surface of the test tube is stored at 4°C for later use;

[0052] (2) Liquid shake flask culture: Cut the slanted strains of the test tube prepared in step (1) into small pieces of 3×3 mm, pick 10 pieces and inoculate them into a 250mL Erlenmeyer flask filled with 150mL liquid shake flask culture medium In this method, the Erlenmeyer flask was cultured on a shaking table for 18 hours under the conditions of a rotating speed of 50 rpm and a temperature of 35°C to produce a liquid shake flask strain; the liquid ...

Embodiment 2

[0065] A method for transforming ganoderma lucidum into grape skin to produce functional food specifically comprises the following steps:

[0066] (1) Expanded culture in test tube: Chizhi ( Ganoderma lucidum ) CICC 14045 test tube slant strains were cut into small pieces of 3×3mm, inoculated a small piece into the test tube slant culture medium, and cultured at 35°C for 4 days to obtain the test tube slant strains, which were stored at 4°C for later use;

[0067] (2) Liquid shake flask culture: Cut the slanted strains of the test tube prepared in step (1) into small pieces of 3×3 mm, pick 3 pieces and inoculate them into a 250mL Erlenmeyer flask filled with 20mL liquid shake flask culture medium Among them, the Erlenmeyer flask was cultured on a shaker for 86 hours at a speed of 200 rpm and a temperature of 20°C to produce a liquid shake flask strain; the liquid shake flask culture medium was sterilized at 100°C for 60 minutes. , and every liter of liquid shake flask cultur...

Embodiment 3

[0080] A method for transforming ganoderma lucidum into grape skin to produce functional food specifically comprises the following steps:

[0081] (1) Expanded culture in test tube: the tree tongue ( Ganoderma applanatum ) GIM5.282 test tube slant strains were cut into small pieces of 3×3 mm, inoculated a small piece into the test tube slant culture medium, and cultured at 28°C for 10 days to obtain the test tube slant strains, which were stored at 4°C for later use;

[0082] (2) Liquid shake flask culture: cut the slanted strains of the test tube prepared in step (1) into small pieces of 3×3 mm, pick 7 pieces and inoculate them into a 250mL Erlenmeyer flask filled with 80mL liquid shake flask culture medium Among them, the Erlenmeyer flask was cultured on a shaker for 57 hours at a speed of 120 rpm and a temperature of 28°C to produce a liquid shake flask strain; the liquid shake flask culture medium was sterilized at 120°C for 35 minutes. , and every liter of liquid shake f...

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Abstract

The invention discloses a method for utilizing ganoderma lucidum to convert grape skin to produce a functional food and relates to the technical field of bioengineering. According to the preparation method, sequentially through the steps of enlarged culture in a test tube, liquid shake-flask culture and enlarged culture in a seed tank, solid fermental cultivation, drying and smashing, the functional food which regulates blood lipids, blood sugar and blood pressure, resists radiation and inhibits tumor is obtained. According to the method, the grape skin, rice, wheat, corn and sorghum and the like are taken as solid substrates, ganoderma lucidum is taken as a strain to convert a grape skin flavone, and ganodenic acid and ganoderma lucidum polysaccharide; in the obtained product, the content of the flavone is 2-50 mg / g of dry substrate, the content of the ganoderma lucidum polysaccharide is 10-100 mg / g of dry substrate, the content of ganodenic acid is 1-20 mg / g of dry substrate, and the method can be used for extracting active ingredients flavones, ganoderma lucidum polysaccharide and ganodenic acid of ganoderma lucidum and producing tablets or capsules for treating the blood lipids, blood sugar and blood pressure, resisting radiation and inhibiting tumor.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for using ganoderma lucidum to transform grape skins to produce functional food. Background technique [0002] Grape skins are leftovers for the production of wine and grape juice. Extracting a variety of biologically active substances from grape skins has been a research hotspot at home and abroad. These active substances have strong antioxidant properties, because they have antioxidant and free radical scavenging properties role has received wide attention. At present, a variety of polyphenol products obtained from grape skins in foreign countries are mainly health care products, medicines, cosmetics and food. In France, Italy, Spain and other wine countries, more than 70% of grape skins can be well utilized . [0003] The research on grape waste in our country has just started. Most of them are used as feed and fertilizer, and some are burned or even discarde...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L19/00A23L7/104A23L31/00A23L33/105A61K36/87A61K36/074A61P3/06A61P3/10A61P9/12A61P39/00A61P35/00C12N1/14C12R1/645
CPCA61K36/074A61K36/87A61K2236/19A61K2236/331A61K2236/333C12N1/14A61K2300/00
Inventor 张志才李琛
Owner JIANGSU UNIV
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