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Application of ganoderic acid Me in tumoral growth or propagation inhibitor

A technology of ganoderma acid and inhibitors, applied in the field of application of ganoderma acid Me in tumor growth or proliferation inhibitors, can solve the problems such as no reports of ganoderma acid monomers, and achieve the ability to curb infinite proliferation, low cytotoxicity, and inhibition The effect of tumor cell proliferation

Inactive Publication Date: 2007-12-12
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the ganoderma acid monomer in the relevant literature

Method used

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  • Application of ganoderic acid Me in tumoral growth or propagation inhibitor
  • Application of ganoderic acid Me in tumoral growth or propagation inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Toxic effects of GA-T and GA-Me on HeLa cells:

[0033] Both GA-T and GA-Me are pure natural products isolated from the mycelium of Ganoderma lucidum produced by fermentation, with a purity greater than 99%, and are diluted with DMEM culture solution to the required concentration. Toxic effects of GA-T and GA-Me on HeLa cells were determined by thiazolium blue (MTT) rapid colorimetric method. Cells in logarithmic growth phase (10 6 cell ml -1 ) was inoculated in a 96-well culture plate, 0.2ml per well, and treated with a certain concentration of GA-T and GA-Me respectively, and each concentration was paralleled to 4 wells, and the control group was added with an equal volume of culture solution, placed at 37 ° C, 5% CO 2Incubate in an incubator with saturated humidity for 1-4 days, add 5mg·ml to each well 4 hours before the end of the experiment -1 MTT 10 μl, add 0.04 N dimethyl sulfoxide (DMSO) to each well after the culture, 150 μl per well, shake for 10 minutes, ...

Embodiment 2

[0036] Toxic effects of GA-T and GA-Me on 95-D cells:

[0037] Both GA-T and GA-Me are pure natural products isolated from the mycelium of Ganoderma lucidum produced by fermentation, with a purity greater than 99%, and are diluted with RPMI-1640 culture solution to the required concentration. The toxic effects of GA-T and GA-Me on 95-D cells were determined by rapid colorimetric method of thiazolium blue (MTT). Cells in logarithmic growth phase (10 6 cell ml -1 ) was inoculated in a 96-well culture plate, 0.2ml per well, and treated with a certain concentration of GA-T and GA-Me respectively, and each concentration was paralleled to 4 wells, and the control group was added with an equal volume of culture solution, placed at 37 ° C, 5% CO 2 Incubate in an incubator with saturated humidity for 1-4 days, add 5mgml to each well 4 hours before the end of the experiment -1 MTT 10 μl, add 0.04 N DMSO to each well after the culture, 150 μl per well, shake for 10 minutes, wait unti...

Embodiment 3

[0040] Cytotoxicity comparison of GA-T and GA-Me on several tumor cells and normal cell L02:

[0041] Both GA-T and GA-Me are pure natural products isolated from the mycelium of Ganoderma lucidum produced by fermentation, with a purity greater than 99%, and are diluted with DMEM or RPMI-1640 culture solution to the desired concentration. The toxic effects of GA-T and GA-Me on several kinds of cells were determined by rapid colorimetric method of thiazolium blue (MTT). Cells in logarithmic growth phase (10 6 cell ml -1 ) was inoculated in a 96-well culture plate, 0.2ml per well, and treated with a certain concentration of GA-T and GA-Me respectively, and each concentration was paralleled to 4 wells, and the control group was added with an equal volume of culture solution, placed at 37 ° C, 5% CO 2 Incubate in an incubator with saturated humidity for 1-4 days, add 5mg ml to each well 4 hours before the end of the experiment -1 MTT 10 μl, add 0.04 N DMSO (dimethyl sulfoxide) ...

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Abstract

The invention provides the uses of ganoderic acid T and ganoderic acid me in preparation of agents for inhibiting the growth and proliferation of tumor cells. In vitro and in vivo studies have shown that ganoderic acid T and ganoderic acid Me have significant effect of inhibiting the growth and proliferation of human tumor cells while have less toxicity on normal cells. It has good application prospect.

Description

[0001] This application is a divisional case of a Chinese patent application with an application date of June 1, 2005, an application number of CN 200510026378.X, and an invention title of "Application of Ganoderma Acid T and Ganoderma Acid Me in Tumor Growth or Proliferation Inhibitors" Apply. technical field [0002] The present invention relates to Ganoderic acid Me and the application of Ganoderic acid T. Background technique [0003] Ganoderma lucidum is a precious medicinal fungus that has been used for thousands of years. In the "Shen Nong's Materia Medica" in the Eastern Han Dynasty, Ganoderma lucidum was listed as a herbal medicine. There are also many treatises on Ganoderma lucidum by medical scientists in the past dynasties, such as "Baopuzi" by Ge Hong in the Eastern Jin Dynasty, "Xinxiu Materia Medica" by Su Jing in the Tang Dynasty, "Bielu of Famous Doctors" by Tao Hongjing in the Liang Dynasty, and "Compendium of Materia Medica" by Li Shizhen in the Ming Dyna...

Claims

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Application Information

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IPC IPC(8): A61K31/575A61P35/00
Inventor 钟建江刘建文唐文
Owner EAST CHINA UNIV OF SCI & TECH
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