Method for producing feedstuff containing ganoderma lucidum active ingredient and feedstuff using ganoderma lucidum liquid fermentation liquid
A technology of feed additives and active ingredients, which is applied in the field of biological fermentation and feed, can solve the problems of multi-step and incomplete utilization of active ingredients, and achieve the effects of maintaining health, increasing immunity and disease resistance, and stimulating the development of the immune system
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Embodiment 1
[0029] Embodiment 1 Ganoderma lucidum fermented liquid preparation and the centrifugation of fermented liquid
[0030] (1) Slant culture: inoculate Ganoderma lucidum strain CGMCC No.5.535 on the slant medium, and cultivate it at 25°C until the mycelium covers the slant. The specific preparation method of the slant medium is: peel the potatoes Cut into small pieces or small pieces, weigh 200 grams, add 500 milliliters of water to boil for 40 minutes, filter with 4 layers of gauze, add 20 grams of glucose to the filtered juice, then add water to 1000 milliliters, then add 20 grams The agar is heated and subpackaged after the agar is completely dissolved, sterilized and set aside.
[0031] (2) Seed culture: insert the strains grown on the slope into the seed culture medium, the volume of the seed culture medium is 100 milliliters, and put into 500 milliliters of triangular flasks, totally 21 bottles. The seed culture medium is (unit is gram per liter): corn flour 20, sucrose 20,...
Embodiment 2
[0034] Example 2 Preparation of Feed Containing Ganoderma Active Components
[0035] According to the ratio of adding 1.0 ml of Ganoderma lucidum fermentation supernatant per kilogram of feed, the pressure spraying process is used to spray the Ganoderma lucidum fermentation supernatant on the surface of feed particles. The operating conditions are: spray pressure: 0.2MPa, material temperature: 20°C, feed rate: 10mL / s. Spraying indicators: the feed mixing uniformity is 0.06%, the particle hardness is reduced by 5%, and the active ingredient loss rate of Ganoderma lucidum is 5.2%. Described feed is typical soybean meal-corn type chicken feed, and its nutrient composition (mass percentage): crude protein 19%, crude fat 3.0%, crude fiber 6.0%, crude ash 8.0%, calcium 1.20%, total phosphorus 0.60% , salt 0.6%; metabolizable energy is 3.0 kcal per gram.
Embodiment 3
[0036] Example 3 Preparation of Feed Containing Ganoderma Active Components
[0037] According to the ratio of adding 0.8 milliliters of ganoderma lucidum fermentation supernatant per kilogram of feed, the ganoderma lucidum fermentation supernatant was sprayed on the surface of feed granules by vacuum spraying process. 30°C. Spraying indicators: the feed mixing uniformity is 0.03%, the particle hardness is reduced by 3%, and the active ingredient loss rate of Ganoderma lucidum is 3.8%. Described feed is typical corn-soybean meal-mixed meal type duck feed, and its nutrient composition (mass percentage): crude protein 15%, crude fat 3.0%, crude fiber 6.0%, crude ash 8.0%, calcium 0.91%, total Phosphorus 0.68%, salt 0.3%; metabolizable energy is 2.8 kcal per gram.
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