Aqueous two-phase system extraction method for ganodenic acid and ganoderma lucidum polysaccharide

A two-phase aqueous system and Ganoderma lucidum polysaccharide technology, applied in steroids, chemical recovery, organic chemistry, etc., to achieve low equipment requirements, shorten extraction time, and reduce costs

Inactive Publication Date: 2010-12-01
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of synchronously extracting ganoderma acid and ganoder

Method used

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  • Aqueous two-phase system extraction method for ganodenic acid and ganoderma lucidum polysaccharide
  • Aqueous two-phase system extraction method for ganodenic acid and ganoderma lucidum polysaccharide
  • Aqueous two-phase system extraction method for ganodenic acid and ganoderma lucidum polysaccharide

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Experimental program
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Embodiment 1

[0030] A two-phase system extraction method of ganoderma acid and ganoderma polysaccharide comprises the following steps:

[0031] Step 1 Raw material pretreatment: take Ganoderma lucidum fruiting bodies, dry at 65°C to constant weight, pulverize, and pass through a 20-mesh sieve to obtain Ganoderma lucidum powder;

[0032] Step 2: Establishment of a two-phase aqueous system: Weigh 43g of ammonium sulfate, add it to 115ml of water, add 64ml of absolute ethanol after dissolution, and mix well to establish an ethanol / ammonium sulfate two-phase system;

[0033] Step 3 Extraction and separation: Add 8g of Ganoderma lucidum powder into 240ml of ethanol / ammonium sulfate two-phase aqueous system, oscillate and shake for 10 minutes, then let stand for 5 minutes to obtain upper phase and lower phase;

[0034] Step 4 Obtaining ganoderma acid: collecting the upper phase, concentrating the upper phase under reduced pressure, taking the concentrated extract, and freeze-drying the concentra...

Embodiment 2

[0037] A two-phase system extraction method of ganoderma acid and ganoderma polysaccharide comprises the following steps:

[0038] Step 1 Raw material pretreatment: take Ganoderma lucidum fruiting bodies, dry at 65°C to constant weight, pulverize, and pass through a 20-mesh sieve to obtain Ganoderma lucidum powder;

[0039] Step 2: Establishment of a two-phase aqueous system: Weigh 15.6g of ammonium sulfate, add it to 32ml of water, add 6.5ml of absolute ethanol after dissolving, and mix well to establish an ethanol / ammonium sulfate two-phase system;

[0040] Step 3 Extraction and separation: Add 2g of Ganoderma lucidum powder into 40ml of ethanol / ammonium sulfate two-phase aqueous system, shake well for 10 minutes, then centrifuge at 5000r / min for 2 minutes to obtain upper phase and lower phase;

[0041] Step 4 Obtaining ganoderma acid: collecting the upper phase, concentrating the upper phase under reduced pressure, taking the concentrated extract, and freeze-drying the conc...

Embodiment 3

[0044] A two-phase system extraction method of ganoderma acid and ganoderma polysaccharide comprises the following steps:

[0045] Step 1 Raw material pretreatment: take Ganoderma lucidum fruiting bodies, dry at 65°C to constant weight, pulverize, and pass through a 20-mesh sieve to obtain Ganoderma lucidum powder;

[0046] Step 2: Establishment of a two-phase aqueous system: Weigh 15.6g of ammonium sulfate, add it to 32ml of water, add 6.5ml of absolute ethanol after dissolving, and mix well to establish an ethanol / ammonium sulfate two-phase system;

[0047] Step 3 Extraction and separation: Add 2g of Ganoderma lucidum powder into 60ml of ethanol / ammonium sulfate two-phase system, in an ultrasonic cleaner, ultrasonically mix at a power of 200W for 2 minutes, and then let stand for 8 minutes to obtain the upper phase and the lower phase;

[0048] Step 4 Obtaining ganoderma acid: collecting the upper phase, concentrating the upper phase under reduced pressure, taking the concen...

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Abstract

The invention relates to an aqueous two-phase system extraction method for ganodenic acid and ganoderma lucidum polysaccharide. The method particularly comprises the following steps of: (1) pulverizing fresh or dry ganoderma lucidum fruiting bodies; (2) establishing an ethanol/ammonium sulfate aqueous two-phase system; (3) performing extraction separation: adding ganoderma lucidum powder into the ethanol/ammonium sulfate aqueous two-phase system, shaking up the mixture by vibration or uniformly mixing the mixture by ultrasonic sound, and standing the mixture for phase splitting or centrifugally splitting the phase to obtain a top phase and a bottom phase; (4) concentrating the top phase under reduced pressure and freeze-drying the top phase to obtain the ganodenic acid; and (5) dialyzing the bottom phase to obtain permeate and intercept fluid, performing alcohol precipitation on the intercept fluid with 80 percent ethanol and centrifuging to obtain a precipitate and supernatant, and free-drying the precipitate to obtain the ganoderma lucidum polysaccharide. The method has the advantages of simple process, low equipment requirement, low cost, no pollution, suitability for batch production and capability of realizing synchronous extraction of the ganodenic acid and the ganoderma lucidum polysaccharide.

Description

technical field [0001] The invention belongs to the extraction method of terpenoids and polysaccharides, in particular to a two-phase system extraction method of ganoderma acid and ganoderma polysaccharides. Background technique [0002] Ganoderma lucidum is a dual-purpose fungus for food and medicine. Folklore: eat Ganoderma lucidum, you can live forever, which fully shows that Ganoderma lucidum does have high medicinal value. It is regarded as a precious Chinese medicinal material and has a history of more than 2,000 years as a medicine. Modern studies have shown that Ganoderma lucidum acid is one of the active ingredients of Ganoderma lucidum that exerts pharmacological effects. It can inhibit the release of cell histamine, enhance the function of digestive organs, lower blood fat, lower blood pressure, protect the liver, and regulate liver function. Tumor, anti-mutation and other effects. Ganoderma lucidum polysaccharide is another active ingredient in Ganoderma lucid...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07J9/00
CPCY02P20/582
Inventor 潘利华姜苏薇罗建平
Owner HEFEI UNIV OF TECH
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