Method for producing feed containing Ganoderma lucidum acid from wheat straw transformed by Ganoderma lucidum

A technology of ganoderma lucidum and wheat straw, which is applied to medical preparations containing active ingredients, animal feed, animal feed, etc., can solve problems such as lack of research and achieve the effect of increasing body weight

Inactive Publication Date: 2008-12-24
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The extraction and property analysis of cellulase produced by liquid fermentation of Ganoderma lucidum were studied, which proved that Ganoderma lucidum can produce cellulase, which further confirmed that Ganoderma lucidum fungus can degrade straw, but they did not conduct in-depth research on the transformation of straw by Ganoderma lucidum cellulase Research

Method used

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  • Method for producing feed containing Ganoderma lucidum acid from wheat straw transformed by Ganoderma lucidum
  • Method for producing feed containing Ganoderma lucidum acid from wheat straw transformed by Ganoderma lucidum

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Effect test

Embodiment 1

[0020] The preparation of embodiment 1 Ganoderma lucidum strain

[0021] The fruiting body of Ganoderma lucidum growing wild in Jiangsu was taken, and after the surface of the fruiting body was sterilized with 75% ethanol, it was moved into an ultra-clean workbench and irradiated with ultraviolet light for 20 minutes. Burn the scalpel on the flame of an alcohol lamp and put it in sterile water to cool down to ensure that the temperature of the scalpel will not damage the fruiting body tissue. Cut out the growth tissue on the edge of the cap, small pieces the size of soybeans, and cut through the surface tissue of the fruiting body. Use sterile tweezers to pick up the slant culture medium (that is, PDA medium) of the test tube, pick up 10 of each tissue, culture in the dark at 25-28°C, and regularly observe the growth and contamination of the mycelium every day. When it grows to 4-10 days, Use the slant of the PDA medium test tube to transfer.

Embodiment 2

[0022] Embodiment 2 preparation containing ganoderma acid feed

[0023] 1. Insert the Ganoderma lucidum strain obtained in Example 1 into the newly prepared potato culture medium (Zhuge Jian, Wang Zhengxiang, Industrial Microbiology Experimental Technical Manual, 1994: P 367), culture temperature 27 ° C, until the mycelium is covered with After the slant, use an inoculation shovel to cut the slant bacteria into a size of 5 × 5mm, and put 8 pieces into a 250mL Erlenmeyer flask containing 60mL shake flask culture medium, and cultivate them on a shaker at 27°C and 150 rpm for 72 hours. Wherein the formula of said shaking flask culture medium is (unit is gram / liter): wheat straw 30, corn flour 10, soybean meal powder 10, KH 2 PO 4 1. MgSO 4 1. The initial pH value is 7.0.

[0024] 2. Put 1L of the cultured strain above into a 25L seed tank equipped with 15L of first-level seed medium. The total fermented liquid in the seed tank is 48.5L; maintain the tank temperature at 27°C, t...

Embodiment 3

[0027] Embodiment 3 preparation containing ganoderma acid feed

[0028] The bacterial classification used in this embodiment is the bacterial classification that embodiment 1 obtains.

[0029] 1. Insert the Ganoderma lucidum strain obtained by the tissue separation method into the newly prepared PDA medium (Zhugejian, Wang Zhengxiang, Industrial Microbiology Experimental Technical Manual, 1994: P 367), culture at 32°C, and wait until the mycelium is full After the slant, the slant strains were inserted into a 250mL Erlenmeyer flask containing 75mL shake flask culture medium, and cultured on a shaker at 30°C and 100 rpm for 172 hours. Wherein the formula of shake flask culture medium is (unit is gram / liter): wheat straw 20, corn flour 15, soybean meal powder 3, KH 2 PO 4 6. MgSO 4 4. The initial pH value is 8.0.

[0030] 2. Insert 1500 mL of strains cultured in shake flasks into a 50 L seed tank equipped with 22.5 L of primary seed medium. The medium is composed of (grams p...

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Abstract

The invention relates to the industrial field of biological feed, in particular to the method of feed containing ganodenic acid produced by converting medicinal ganodermalucidum into cornstock, and the effect of the functional feed produced through the method. In the invention, liquid submerged culture, seeding tank extended culture and solid-state fermentation culture are conducted with the ganodermalucidum as the starting bacterial strain and the cornstock as the auxiliary material, to obtain the functional feed rich in ganodenic acid. The feed of the invention is light yellow powder and has fragrance; the content of crude ganodenic acid in the feed is up to 0.06 percent to 0.3 percent, and the content of protein is 12 percent to 14 percent. The feed containing ganodenic acid can be used as the additive of animal feed and for preparing medicine or health care food which have the functions of regulating blood sugar and lipid, and preventing cardio-cerebrovascular diseases or bronchitis. The invention not only develops a new approach for deeply developing and utilizing the ganodermalucidum, but also provides ideal biological additive for developing the functional feed.

Description

technical field [0001] The present invention relates to the field of biological fermentation and feed, specifically, the present invention relates to a method for producing feed containing ganoderma acid by transforming wheat straw with medicinal fungus ganoderma lucidum. Feed additives have a variety of biological functions, and can be used to improve animal body immunity and enhance the anti-virus ability of reared animals. The ganoderma acid produced with this feed as a raw material can also be used as a medicine or health food for regulating blood sugar and blood lipids and preventing cardiovascular and cerebrovascular diseases or bronchitis. Background technique [0002] With the improvement of people's quality of life, more and more people begin to pay attention to the safety of livestock and poultry products. Developing healthy breeding and ensuring animal health is a complex systematic project involving many aspects. In the past, in order to enhance the resistance o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23L1/29A23L1/28A61K36/074A61P9/00A61P3/10A61P3/06A61P11/04A23K10/12A23K10/37A23L31/00
CPCY02P60/87
Inventor 张志才黄达明崔凤杰何林富
Owner JIANGSU UNIV
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