Method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation

A technology of solid fermentation and dendrobium slag, applied in the field of microbial fermentation, can solve problems such as no research reports, and achieve the effect of all-round comprehensive utilization

Active Publication Date: 2013-08-14
SHAOXING RULIN BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] During the deep processing of Dendrobium candidum products, some fibrous residues will inevitably be produced. How to realize the efficient utilization of these processing wastes is an important component of the deep comprehensive utilization of Dendrobium candidum, but there is no research on this aspect at present to report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Slant culture: inoculate the ganoderma lucidum strains on the slant medium (i.e. PDA medium), and cultivate it at 30°C for about 7 days until the mycelium covers the slant;

[0023] (2) Liquid seed culture, the slant bacteria were inoculated into a 250-milliliter Erlenmeyer flask equipped with 80 mL of seed medium, the composition of the seed medium (unit gram / liter): glucose 20, corn flour 20, bran 10, K H 2 PO 4 1. MgSO 4 ·7H 2 O2, natural pH, sterilized at 121°C for 20 minutes, cultured at 30°C for 7 days on a shaker with a rotation speed of 150 rpm;

[0024] (3) For solid fermentation culture, 10 mL of cultured seeds were inoculated into a 500 mL Erlenmeyer flask containing 100 g (dry material weight) of fermentation medium. Composition of fermentation medium: dry material 100g (Dendrobium slag 98g, bran 1.9g, KH 2 PO 4 0.1g), feed-to-water weight ratio 1:1, sterilized at 121°C for 30 minutes; cultured at 22°C for 10 days, the polysaccharide content was ...

Embodiment 2

[0032] Fermentation medium material water weight ratio 1: 1.2, sterilized at 121°C for 30 minutes; cultivated at 25°C for 10 days, other conditions were the same as Example 1, the polysaccharide content measured at the end of fermentation was 1.85%, and the ganoderma acid content was 0.472%.

Embodiment 3

[0034] Fermentation medium material water weight ratio 1: 1.2, sterilized at 121°C for 30 minutes; cultivated at 28°C for 15 days, other conditions were the same as in example 1, the content of mycelia polysaccharide measured at the end of fermentation was 2.75%, and the content of ganoderma acid was 0.518 %.

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Abstract

The invention relates to the microbial fermentation field and discloses a method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation. The method utilizes dendrobium slag as medium to perform the solid fermentation of the ganoderma and is achieved through the following steps of (1) slant cultivation, (2) seed cultivation and (3) solid fermentation cultivation. The cultured seeds are inoculated into a solid fermentation culture medium at an inoculation size of 10%. The dry materials of the solid fermentation culture medium are composed of 98% of the dendrobium slag, 1.9% of bran and 0.1% of potassium hydrogen phosphate and the weight ratio of the dry materials and water is 1: (1-2.3). The ganoderma is cultivated for 7-20 days at a natural pH value in a temperature condition of 22-32 DEG C. According to the method of utilizing the dendrobium candidum slag for cultivating ganoderma through the solid fermentation, the cellulose in the dendrobium slag is decomposed and utilized by the ganoderma through the fermentation and the biotransformation of the ganoderma to generate high-efficiency bioactive materials such as ganoderma polysaccharide and ganoderma acid, and the deep-processing waste of dendrobium products is converted into high value-added products, so that the all-around comprehensive utilization of dendrobium raw materials is achieved.

Description

Technical field [0001] The present invention involves the field of microbial fermentation, and especially involves a method that uses Dendrobium Fer Dendrobium slag to ferment Ganoderma lucidum. Background technique [0002] Dendrobium is also known as Dendrobium. The origin is mainly distributed in Tropical and subtropicals in Asia, Australia and Pacific Islands, with about 1,000 types in the world.Of the 76 Dendrobium plants in China, nearly 40 are used for medicine.Among them, Dendrobium candidum is the best in Dendrobium, which is listed as "the head of the nine major fairy grass in China".The tender stems of Dendrobium candidum are processed by spiral or springs, and they are dried. [0003] In recent years, with the rise of green consumption, especially people's attention to the safety of artificial synthetic chemicals, it has prompted natural products to pay more attention in the international academic community.Dendrobium candidum is a kind of natural medicine and food -l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05F5/00C05G1/00
CPCY02A40/20
Inventor 陈乃伟丁重阳
Owner SHAOXING RULIN BIOTECH
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