Method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation
A technology of solid fermentation and dendrobium slag, applied in the field of microbial fermentation, can solve problems such as no research reports, and achieve the effect of all-round comprehensive utilization
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Embodiment 1
[0022] (1) Slant culture: inoculate the ganoderma lucidum strains on the slant medium (i.e. PDA medium), and cultivate it at 30°C for about 7 days until the mycelium covers the slant;
[0023] (2) Liquid seed culture, the slant bacteria were inoculated into a 250-milliliter Erlenmeyer flask equipped with 80 mL of seed medium, the composition of the seed medium (unit gram / liter): glucose 20, corn flour 20, bran 10, K H 2 PO 4 1. MgSO 4 ·7H 2 O2, natural pH, sterilized at 121°C for 20 minutes, cultured at 30°C for 7 days on a shaker with a rotation speed of 150 rpm;
[0024] (3) For solid fermentation culture, 10 mL of cultured seeds were inoculated into a 500 mL Erlenmeyer flask containing 100 g (dry material weight) of fermentation medium. Composition of fermentation medium: dry material 100g (Dendrobium slag 98g, bran 1.9g, KH 2 PO 4 0.1g), feed-to-water weight ratio 1:1, sterilized at 121°C for 30 minutes; cultured at 22°C for 10 days, the polysaccharide content was ...
Embodiment 2
[0032] Fermentation medium material water weight ratio 1: 1.2, sterilized at 121°C for 30 minutes; cultivated at 25°C for 10 days, other conditions were the same as Example 1, the polysaccharide content measured at the end of fermentation was 1.85%, and the ganoderma acid content was 0.472%.
Embodiment 3
[0034] Fermentation medium material water weight ratio 1: 1.2, sterilized at 121°C for 30 minutes; cultivated at 28°C for 15 days, other conditions were the same as in example 1, the content of mycelia polysaccharide measured at the end of fermentation was 2.75%, and the content of ganoderma acid was 0.518 %.
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