261 results about "Lingzhi mushroom" patented technology

The invention provides a method for interplanting ganoderma lucidum in tea gardens. The method includes the steps that ganoderma lucidum bacterial strains which can adapt to strong light for growth and appropriate compost are selected, compost mixing, bagging, sterilization, inoculation and mycelium culture are conducted, and after bags are filled with mycelia and colors of the mycelia are changed, ganoderma lucidum wild imitation substitute earthing cultivation is conducted in a tea garden ecological environment. By the adoption of the novel method for cultivating the ganoderma lucidum, the quality of the ganoderma lucidum can be improved, conventional greenhouse investment for substitute earthing ganoderma lucidum cultivation is reduced, the operating process is simplified, the economic output value of the tea gardens is increased, soil fertility of the tea gardens can be also improved, the yield and the quality of tea leaves are improved, production and income of the tea gardens are promoted, and the method has remarkable economic and social benefits and is suitable for being massively popularized and applied.

A method for using ganoderma lucidum liquid fermentation liquor to prepare feed and feed additive containing ganoderma lucidum active ingredient, pertains to the field of biofermentation and feed. The invention includes steps of: a. centrifugally separating the ganoderma lucidum liquid fermentation liquor at 5000 per-minute to obtain the ganoderma lucidum fermentation supernatant and ganoderma lucidum residues; b. adopting a rear spraying process to mixing the ganoderma lucidum fermentation supernatant with the feed in a proportionality of adding 0.5-1.5 ml of the supernatant in the feed for preparing feed containing ganoderma lucidum active ingredient; c. performing a second fermentation to the ganoderma lucidum residues for preparing the feed additive containing ganoderma lucidum active ingredient. The invention fully use the ganoderma lucidum fermentation liquor, has no waste water and waste slag discharge, simple steps, low cost, and easy large-scale industrialization. Products are rich in Ganoderma lucidum polysaccharides and ganoderma lucidum acid; have a balance immune system, and antibacterial, antiviral, anti-parasitic, anti-tumor, anti-radiation and the like extensive physiology activities, thereby largely improving the immune disease resistance, maintaining the animal intestinal health, and further improving the production performance and market efficiency of animals.

The present invention relates to a kind of active component extracted from glossy ganoderma spore as material and its preparation and use. The glossy ganoderma spore oil contains total triterpene in 24-35 %, has density of 0.88-0.92, refractive index 1.45-1.48 and acid number 14-35. The preparation process with wall breaking glossy ganoderma spore as material includes mixing with alcohol solution, pelletizing, drying, supercritical CO2 extraction in extracting reactor, two-stage separation, and extraction to obtain oily matter, and eccentric separation to obtain yellow oil. The glossy ganoderma spore oil product is used in preparing medicine and food for raising body's immunity. The process of the present invention is simple, stable in product quality, convenient and high in product yield, and is suitable for industrial production.

The invention belongs to the field of drinks, in particular to chrysanthemum tea and a making method thereof. The chrysanthemum tea is made with, by part, 80-100 parts of chrysanthemum flower, 8-12 parts of pear flower, 3-5 parts of lily flower, 3-5 parts of lotus leaf, 3-5 parts of cassia seed, 2-4 parts of wolfberry fruit, 1-3 parts of Lingzhi mushroom, 4-6 parts of wax gourd, 2-4 parts of lemon, 2-4 parts of honeysuckle flower, 3-5 parts of Pu'er tea, and 6-10 parts of crystallized honey. The chrysanthemum tea tastes fine and smooth, has long-lasting aroma, and can be consumed often to benefit physical health and improve body immunity since pear flower, lily flower, lotus leaf, cassia seed, wolfberry fruit, Lingzhi mushroom, wax gourd, lemon, honeysuckle flower and Pu'er tea are introduced in the chrysanthemum tea.

The invention discloses a method for increasing the content of intracellular ganoderma lucidum triterpenes during liquid fermentation of ganoderma lucidum mycelia. The method is characterized by comprising the steps of performing enlarged cultivation, adding a wood degradation product into a culture solution according to a mass proportion of 0.01 to 5 percent, and performing culture for 1 to 6 days. According to the method for increasing the content of the intracellular ganoderma lucidum triterpenes during liquid fermentation of the ganoderma lucidum mycelia, microcrystalline cellulose is added to induce ganoderma lucidum to produce more intracellular ganoderma lucidum triterpenes during liquid fermentation of the ganoderma lucidum mycelia; the adopted inducer microcrystalline cellulose is safe and low in cost, and the content of the intracellular ganoderma lucidum triterpenes can be remarkably increased.

The invention relates to the microbial fermentation field and discloses a method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation. The method utilizes dendrobium slag as medium to perform the solid fermentation of the ganoderma and is achieved through the following steps of (1) slant cultivation, (2) seed cultivation and (3) solid fermentation cultivation. The cultured seeds are inoculated into a solid fermentation culture medium at an inoculation size of 10%. The dry materials of the solid fermentation culture medium are composed of 98% of the dendrobium slag, 1.9% of bran and 0.1% of potassium hydrogen phosphate and the weight ratio of the dry materials and water is 1: (1-2.3). The ganoderma is cultivated for 7-20 days at a natural pH value in a temperature condition of 22-32 DEG C. According to the method of utilizing the dendrobium candidum slag for cultivating ganoderma through the solid fermentation, the cellulose in the dendrobium slag is decomposed and utilized by the ganoderma through the fermentation and the biotransformation of the ganoderma to generate high-efficiency bioactive materials such as ganoderma polysaccharide and ganoderma acid, and the deep-processing waste of dendrobium products is converted into high value-added products, so that the all-around comprehensive utilization of dendrobium raw materials is achieved.

The invention discloses Niugu health dried noodles. The Niugu health dried noodles comprise 100-110 parts of wheat flour, 20-30 parts of oat flour, 10-15 parts of ground peanut flour, 2-4 parts of rehmannia leaf, 3-4 parts of root of Kirilow Rhodiola, 13-20 parts of ox bone, 2-3 parts of glossy privet fruit, 2-3 parts of turmeric, 3-4 parts of garlic, 2-3 parts of peach leaf, 2-3 parts of boxthorn leaf, 1-2 parts of Chinese hawthorn seed, 2-4 parts of pomelo peel, 2-3 parts of lotus leaf, 1-2 parts of lingzhi mushroom, salt, and the proper amount of water. The Niugu health dried noodles are smooth to taste and can be eaten without causing burning up and damp increase to human body. The Niugu health dried noodles contain nutrients, and resources are saved and production cost is lowered by adding the ox bone. A making method of the Niugu health dried noodles is slightly destructive to nutrients in the ox bone, and the nutrients of the ox bone are fully retained in the product and protect human cell tissue. The root of Kirilow Rhodiola is anti-aging, anti-hypoxic, anti-fatigue, radiation-proof, anti-tumor and anti-viral, enhances brain function, and improves myocardial function.

The present invention discloses one kind of compound glossy ganoderma spore oil and its preparation process. Compound glossy ganoderma spore powder as material is dry pressed to palletize and extracted in a extracting kettle with supercritical CO2 or ultrasonically to obtain pale yellow compound glossy ganoderma spore oil. The compound glossy ganoderma spore oil contains total organic acid over 90 % and total ganoderic acid in 0.1-2.5 %, and has acid value below 25. The process of the present invention is simple, convenient and suitable for industrial production, and the prepared compound glossy ganoderma spore oil is used in medicine and food.

The invention relates to bio-pharmaceutical production technology, in particular to a novel culture medium formulation of glossy ganoderma mycelium fermentation and an optimization method. According to the mass percent, the formulation comprises the components: 0.8-2.0% of tartary buckwheat, 0.5-2.5% of coix seed, 0.5-2.0% of glucose, 0.5-3.0% of soybean flour, 0.1-0.5% of yeast extract, 0.01-0.2% of monopotassium phosphate, 0.01-0.1% of magnesium sulfate and 98.08-89.70% of water; the optimization method is operated according to the following sequence: glossy ganoderma strain is determined; the formulation 1 which is most suitable for the conventional culture medium is determined; the optimal formulation 2 of buckwheat culture medium is determined; the optimal formulation 3 of coix seed culture medium is determined; the optimal formulation 4 of culture medium which is formed by simultaneously adding the buckwheat and the coix seed in the formulation 1 is determined; the optimized final formulation 5 is obtained. The invention aims at providing the novel culture medium formulation of glossy ganoderma polysaccharide fermentation and the optimization method which have low cost, rich micro-factors and reasonable trophic structure, and can effectively increase the yield of glossy ganoderma polysaccharide fermentation.

The invention discloses an enzymolytic preparation method for glossy ganoderma amylose which comprises the steps of, employing inclined-plane bacterial gradual cultivation to obtain the glossy ganoderma fungi fermentation liquid, adjusting fermentation liquid pH and temperature, carrying out enzyme decomposition to the glossy ganoderma mycelia by means of self enzyme group existed in glossy ganoderma mycelia, charging into the fermentation liquor by cellulase, beta-dextranase, pectin enzyme and neutral proteinase, carrying out hydrolytic decomposition, enzyme eradication, filtering, concentrating, alcohol depositing and separating.

The invention provides a method for preparing high-purity lucid ganoderma hyphostroma powder through liquid submerged fermentation. The method solves the problems that the existing solid fermentation period of lucid ganoderma hyphostroma is long, the lucid ganoderma hyphostroma is prone to being contaminated by bacteria, the quality of the final product is unstable, and the content of active ingredients is low. The method includes the steps of culture medium preparation, strain activation, inoculation and culture, fermentation expansion, concentration, homogenization, spray drying and the like. The culture medium preparation step includes the procedures of A, agar slant culture medium preparation, B, shake flask culture medium preparation, C, seeding tank culture medium preparation and D, fermentation tank culture medium preparation. The method is widely used for processing lucid ganoderma hyphostroma powder in the food industry.

A method utilizing protoplasts to fuse and screen rich-selenium high-yield strains among different ganoderma varieties belongs to the technical field of biological husbandry. The method selects different ganodermas to produce strains, the ganoderma cultivation is carried out, the ganoderma strains with high ganoderma sporocarp yield and good product agronomic trait are selected as the high-yield fusion original strains; and the selenium-resistant experiment is utilized to screen the ganoderma strains with strong rich-selenium capability as the rich-selenium fusion original strains. The method determines one parent strain as the inactivation strain through the hyphal growth speed and form, pigmental secretion, protoplast regeneration rate, protoplast inactivation time and the like; then the fusant screening is carried out through the hyphal form and antagonism, so the operation simplicity and practicability are obtained. The method is adopted to fuse one ganoderma fusion strain which has the sporocarp selenium content and yield higher than those of the original strain, so a good economical benefit is created.

The invention discloses a ganoderma lucidum pictographic miniascape and a cultivation method of pictographic works of art. Through shape constraint, a red cheese culture medium and sporocarps are shaped to needed shapes. Films are covered after hypha grows full of a bag and feels hard. After mycoderma is cultivated to turn to brown, the covered films are opened, and ganoderma lucidum outlet is covered by the films and ganoderma lucidum outlet cultivation is conducted. After a white growth circle on the edge of the ganoderma lucidum fungus lid turns to light brown and spores are not sent out, a ventral side of the ganoderma lucidum is stuck by film pieces until the ventral side becomes thick and hard. After process, viewing works of art are formed through combination of the ganoderma lucidum culture medium and the sporocarps. The product appearance of the ganoderma lucidum pictographic miniascape is peculiar, color and luster is bright, and longer vigor of life can be maintained. Hypha in a culture medium with a thin wax coating surface can have vigor of life for a long time and a viewing effect can be further improved. The processed sporocarps and culture medium is hard in surface, attractive in color and luster and can be stored for a long time.

A multi-fungus composition for improving immunity and preventing and treating cancer is prepared from two or more of 14 edible funguses including champignon, genoderma, Jinzheng mushroom, etc through culturing. Its preparing process is also disclosed.

The invention discloses a lucid ganoderma-probiotic composite fermentation product and a preparation method and application thereof. The lucid ganoderma-probiotic composite fermentation product comprises a lucid ganoderma fermentation supernatant, a probiotic fermentation liquid and functional nutritional ingredients. The preparation method comprises the following steps: fermenting lucidum ganoderma mycelia, carrying out centrifuging, taking the supernatant, and mixing the supernatant with the functional nutritional ingredients; then adding the probiotic fermentation liquid into a mixture obtained in the previous step, and carrying out fermentation; and finally, subjecting a fermentation product to inactivation or centrifugation to obtain the composite fermentation product. The composite fermentation product contains rich nutritional ingredients such as polysaccharides, polypeptides and organic acids and is easily and fully absorbed by the skin, and the composite fermentation product contains ingredients such as microelements which can promote repair and recovery of injured skin, so the composite fermentation product has a good skin care effect. The composite fermentation product is beneficial for skin health and is not polluted by enzymes, chemicals and the like; the whole fermentation system is mild in conditions; and the preparation method is simple to operate and controllable in cost, and is beneficial for large-scale production.

The invention discloses a lucid ganoderma hypha powder or lucid ganoderma tea and dual ferment technology, which comprises the following parts: lucid ganoderma mother kind, liquid hypha and ferment culture medium, wherein the lucid ganoderma mother kind adapts the improved PDA culture medium; the method adapts traditional fungal lucid ganoderma and high-activity yeast to proceed dual ferment based on main raw material of good-quality soybean or tea, which proceeds oxygen-enriched ferment for 1-4 h at 20-40 deg.c; the material is deactivated for 40-120 min at 0.08-0.15Mpa, which is switched-in the lucid ganoderma hypha to ferment for 20-40 days; when the hypha is dried and grinded, the product is formed. The invention displays high-product rate, which reduces the cost.

The invention relates to a preparation method of strain fermented tea. The preparation method comprises the following steps: domesticating a lucid ganoderma strain by using a tea domestication culture medium, inoculating the domesticated lucid ganoderma strain on the tea to ensure that the tea is fermented, and drying the fermented tea to obtain the strain fermented tea. According to the preparation method, by organically combining the lucid ganoderma and the tea, tea contains effective ingredients of the lucid ganoderma, the health performance of the tea is improved, and the mouth feels of bitterness and astringency of the tea are improved.

The invention provides a culture method and applications of Ganoderma weberianum mycelial pellets, belonging to the biological technical field. The culture method comprises the following steps: preculturing on a shelving medium slant to activate strains, transferring the activated strains to plating medium to culture and obtain plating strains; and transferring the plating strains to liquid medium to perform shaking culture under controlled conditions and obtain the mycelial pellets. The mycelial pellet culture prepared by the method is mixed with 0.5-10 times of indigo solution or denim printing and dying wastewater by volume to perform shaking culture for decoloration and degradation under the conditions that the pH value is 3-7, the temperature is 13-38 DEG C and the rotational speed is 160rpm or the conditions that the pH value is 2-6, the temperature is 23-43 and the rotational speed is 160rpm. The invention has the following advantages: the cell immobilization cost is saved, industrial production is easy to realize; decoloration and degradation can be performed without using toxic and expensive medium, thus the method is green and environmentally friendly; and the service life of the mycelial pellets is long, the mycelial pellets can be used repeatedly; and the method is convenient and extensive to operate and the cost is low.

Take tea as matrix double fermentation production ganoderma lucidum mycelium and its preparation method, ganoderma lucidum mycelium's Mars effective component for tea polyphenol 100~~250g / Kg, ganoderma lucidum polysaccharide 20~~30g / Kg, protein 200~~400g / Kg, vitamin 40~~100g / Kg. Its preparation method is, uses double lives the fermentation, the single layer vaccinates the yeast in the tea foundation, ferments through the solid state, obtains including the tea polyphenol, the inorganic mineral substance element, the vitamin, the yeast egg waits in vain the many kinds of active nutrition metabolism product, the tea area south of Yellow River has has the side effect to the human body Coffee Alkalito obtain the decomposition, the content reduces. Double in in above metabolism product foundation, after deactivation vaccinates the ganoderma lucidum fungus, the second solid state ferments, obtains including the tea polyphenol, massive organic mineral substance element, many kinds of vitamins, amino acid, protein, ganoderma lucidum polysaccharide and so on many kinds of effective active constituent ganoderma lucidum mycelium. He retained the tea unique flavor and the feeling in the mouth, after causes the processing the broken tea leaves to recycle waste.

The invention discloses glossy ganoderma fermentation filtrate by using panax ginseng as culture mediums and an application of the glossy ganoderma fermentation filtrate to cosmetics. The fermentationfiltrate is supernatant which is obtained through the steps of mixing effective components of panax ginseng, water, glossy ganoderma mycelia and other nutrient components to obtain an initial system,performing fermentation to obtain fermentation liquid, performing sterilization and performing centrifugation. According to the glossy ganoderma fermentation filtrate disclosed by the invention, thepanax ginseng is subjected to whole plant fermentation through the glossy ganoderma mycelia, so that all the efficacy components and the activity thereof of plants are reserved, and running off of active components, caused by an extraction method, is avoided; the active components in the panax ginseng, such as ginsenoside, are subjected to biotransformation through enzymes in the glossy ganodermamycelia, so that the skin care efficacy of the glossy ganoderma fermentation filtrate is improved. The conditions of fermentation temperature, pH and the like are mild, essence is not added, and the safety of products is guaranteed. Besides, fermentation products have natural fresh medicine fragrance. The product namely the glossy ganoderma fermentation filtrate is rich in active components of theginsenoside, ganoderan, ganoderma triterpenes and the like, can adjust the immunity of skin, and has the skin care functions of preserving moisture and resisting ageing.

The invention discloses a brewing method for ganoderma lucidum-black glutinous rice wine, and ganoderma lucidum, black glutinous rice and honey are taken as main raw materials for careful brewing of the low-alcohol health wine. Liquid submerged culture is adopted, mycelia of ganoderma lucidum are produced, after hydrolysis, high-quality black glutinous rice, black dates and honey are matched, modern organisms are applied for multi-strain and multi-time fermentation to prepare effective components, Yibin best-quality sorghum Baijiu is added to strengthen the effective components, then a Chinese most ancient cooking fermentation technology is used, and the unique-style 12% vol low-alcohol leisure wine is produced; the wine has the efficacies of reducing blood lipid, prolonging life, enhancing human body immunity and the like, has the appearance and the taste meeting demand of people on traditional wine drinking, and successfully reduces formed ethanol damage of high-alcohol ethanol on human brains and livers; and the wine can be regarded as the low-alcohol leisure health-preserving wine.

The invention relates to a Ganoderma amboinense bacterial liquid fermentation culture method. The method comprises the following steps: (1) mother strain amplification: taking a pat of Ganoderma amboinense as a mother strain under aseptic conditions, transferring the mother strain into a slant culture medium, adding a plug, putting into a constant-temperature incubator, and culturing at 25-29 DEG C for 7 days; (2) preparation of seed liquid: taking the mycelia from the slant culture medium, inoculating the mycelia into a seed solution culture medium, putting the culture medium in a shaking table, and culturing at 25-29 DEG C at the rotating speed of 140-160 r / min for 7 days; and (3) fermentation in fermentation tank: inoculating the seed solution into the fermentation tank according to the inoculum size of 10%, and carrying out constant-temperature culture at 25-29 DEG C for 80 hours, wherein the stirring rate in 36 hours is 90-110 r / min, the stirring rate from 36 hours to 70 hours is 110-130 r / min, and the stirring rate from 70 hours to 80 hours is 90-110 r / min. After the fermentation, the Ganoderma amboinense fermentation liquid contains higher contents of Ganoderma lucidum polysaccharides and Ganoderma lucidum triterpenoids.

The invention relates to a Chinese medicine plaster for treating diseases in muscles and bones, in particular to a plaster for promoting muscles and bones, and preparation and application thereof. The plaster is prepared by mixing pastes and powders; wherein the raw materials of the pastes by weight comprise: 25-60g of clematis root, 25-60g of heracleum, 25-60g of incised notopterygium rhizome, 25-60g of erycibe obtusifolia, 25-60g of garden balsam stem, 25-60g of gentiana macrophylla, 25-60g of prepared common monkshood daughter root, 25-60g of dried ginger, 25-60g of cinnamon, 25-60g of harlequin glorybower leaf and twig, 25-60g of rhizoma corydalis, 25-60g of ligusticum wallichii, 25-60g of frankincense, 25-60g of cowherb seed, 25-60g of lingzhi mushroom, 25-60g of gastrodia elata and 25-60g of astragalus, 25-60g of one or more of honeycomb, radix aconiti brachypodi and nux vomica, 0.1-3kg of minium and 0.5-5kg of vegetable oil; and the raw materials of the powders by weight comprise: 15-45g of clematis root, 15-45g of heracleum, 15-45g of incised notopterygium rhizome, 15-45g of phellodendron amurense, 15-45g of dragon's blood, 15-45g of gastrodia elata, 15-45g of nux vomica, 15-45g of rhizoma corydalis, 15-45g of borneol, 15-45g of harlequin glorybower leaf and twig and 15-45g of common monkshood mother root. The plaster for promoting muscles and bones has balanced effect, high medicine utilization ratio and good treatment effect.

The invention discloses a method for producing high ganoderma triterpenes content ganoderma lucidum mycelium. After strain culture is finished, strains undergo the shaking table culture, primary amplification, secondary amplification and fermentation cultivation process and finally undergo filter pressing and drying smashing to obtain the high ganoderma triterpenes content ganoderma lucidum mycelium. The method has the advantages that a culture medium formula and a fermentation culture medium formula are optimized according to strain design, a production process is improved, and the functional component content of ganoderma triterpenes is greatly improved and can be more than twice that of ordinary ganoderma lucidum. The method for producing the high ganoderma triterpenes content ganoderma lucidum mycelium is performed at 26+ / -1 DEG C from the culture, the whole production process is free of acid-bases and organic solvents, active functional components are protected to the largest extent, and the method is favorable for the absorption and utilization by the human body and can bring the pharmacological effects of ganoderma lucidum into play to the largest extent.

The invention relates to ganoderma lucidum tea and a preparation method thereof, belonging to the field of health-care products. The ganoderma lucidum tea is of granulated shape and comprises the components of ganoderma capense, ganoderma sinense, ganoderma lucidum Karst and raw ganoderma spore powder in the mass ratio of (5-20):(3-10):(2-10):1. Since the ganoderma capense, the ganoderma sinense,the ganoderma lucidum Karst and the ganoderma spore powder of the ganoderma sinense and the ganoderma lucidum Karst are selected as raw materials, the defects of uneven active component contents of asingle ganoderma lucidum raw material or much low active component contents of a part of raw materials are overcome, the complementary advantages of efficacies of different ganoderma lucidums are given to full play and the indication range of the ganoderma lucidum tea is expanded. The ganoderma lucidum tea is prepared with a special process by utilizing pure lucid ganoderma as the raw material without adding any auxiliary material, wherein a pharmaceutic adjuvant is replaced with the sporoderm-unbroken raw ganoderma spore powder as a pelletizing adhesive, and originally nonviscous ganoderma lucidum stipe and pileus as raw materials are prepared into ganoderma lucidum pulvis which is convenient for packaging, storage and brewing.

The invention relates to the field of fungus fermentation, in particular to ganoderma mycelium fermentation liquor with a high ganoderma polysaccharide content and a method for preparing the ganodermamycelium fermentation liquor. The method includes carrying out slant tube culture on ganoderma strains to obtain mycelium blocks; inoculating the mycelium blocks to plate culture media, carrying outmycelium culture, then switching the mycelium blocks to malt culture media; carrying out liquid fermentation culture at the temperature of 25-29 DEG C for 3-7 days. The ganoderma mycelium fermentationliquor and the method have the advantages that mycelium liquid fermentation processes are adopted, accordingly, the ganoderma mycelium fermentation liquor is high in ganoderma polysaccharide content,fermentation processes are environmentally friendly and simple and are low in cost, short in required time and convenient to implement, and the ganoderma mycelium fermentation liquor and the method are suitable for large-scale industrial production and convenient to popularize.

The invention belongs to the field of fungus cultivation and provides a lucid ganoderma fungus purification and rejuvenation cultural method and a culture medium thereof against the problem that the activity of lucid ganoderma fungi is lowered due to an existing traditional lucid ganoderma fungus culture medium. The cultural method comprises the following steps: 1, preparing the lucid ganoderma fungus rejuvenation culture medium, wherein the lucid ganoderma fungus rejuvenation culture medium is prepared from 150-400 g of potatoes, 10-30 g of glucose, 10-25 g of agar powder, 1-5 g of monopotassium phosphate, 0.5-3 g of magnesium sulfate and 30-70 g of lucid ganoderma sporocarp; 2, culturing lucid ganoderma fungi into hypha, and then inoculating the hypha to the lucid ganoderma fungus rejuvenation culture medium obtained in step 1; 3, conducting culturing according to normal conditions, and obtaining rejuvenation fungi. The hypha obtained through the cultural method is quick in growth, pure white, orderly and dense, and the obtained sporocarp is good in agronomic trait.

The invention relates to the technical field of microbes and particularly relates to a method for extracting polysaccharides from ganoderma lucidum mycelia. The method comprises the steps of mixing dried ganoderma lucidum mycelia with water, firstly, carrying out uniform mixing by a high-speed homogenizer, then, carrying out homogeneous dispersion for 1 to 5 times by high pressure of 500bar to 1500bar, and then, carrying out centrifugation, thereby obtaining polysaccharide-containing supernatant. Compared with the traditional water extraction method, the method has the advantages that the yield of the ganoderma lucidum polysaccharides can be increased, the time of extraction of the ganoderma lucidum polysaccharides is shortened, and the oxidation resistance and anti-inflammation effect ofthe ganoderma lucidum polysaccharides are improved.

A stereoscopic cultivation and breeding method for complementary symbiosis of ganoderma lucidum and wood frogs is characterized by comprising the steps that a good, pure-natural and pollution-free area section is selected, it is ensured that the area section is free of residues, soil is not too alkaline and the area section is a loose sandy soil land section, deep land scarification is performed for 40 centimeters in the first year, leveling is performed, and a ganoderma lucidum and wood frog greenhouse is constructed after six months of sun exposure.Too large area is not allowed, the area of the greenhouse is 200 square meters, the length of the greenhouse is 25 meters, the width of the greenhouse is 8 meters, the height of the greenhouse is 1.9 meters, the area is suitable for a growing environment for the complementary symbiosis of the ganoderma lucidum and the wood frogs, the greenhouse is suitable for conidial powder recovery, the area of the greenhouse is suitable for the wood frogs due to the fact that wood frogs breathe through the skins, the ganoderma lucidum breathes freely, the wood frogs uptake oxygen, the complementary symbiosis is achieved, and the greenhouse is disinfected and sterilized with quick lime water after the greenhouse construction is completed; ganoderma lucidum mycelium in the step 2 is ganoderma lucidum.

The invention belongs to the technical field of ganoderma lucidum culture, and particularly discloses a method for culturing ganoderma lucidum in a high-altitude area and a method for preparing health-care products of ganoderma lucidum. Traditional Chinese medicine components comprise cordyceps sinensis, shiitake mushrooms, fructus schizandrae, rhodiola rosea, radix aconite lateralis, ganoderma lucidum, rhizoma dioscoreae, radix ginseng, cornu cervi, hippocampus japonicus and musk; a culture solution for culturing ganoderma lucidum is prepared through treatment; ganoderma lucidum is cultured in the high-altitude area by utilizing the culture solution, minerals, trace elements and other nutrients in the culture solution can be effectively absorbed by thalli of ganoderma lucidum, the nutrients of ganoderma lucidum can be added, the content of the nutrients of ganoderma lucidum can be increased, the growth state of ganoderma lucidum can also be improved, and the yield is increased; in addition, the prepared health-care products of ganoderma lucidum have the effects of treating constipation, chronic gastritis, gastroenteritis, diarrhea, ovarian cyst and the like.