Novel culture medium formulation of glossy ganoderma mycelium fermentation and optimization method

A culture medium formula, Ganoderma lucidum mycelium technology, applied in the directions of organic fertilizers, fertilizer mixtures, fertilization devices, etc., can solve the problems of high carbon source cost, lack of trace factors, unfavorable fermentation yield of Ganoderma lucidum polysaccharides, etc., and achieves convenient operation, Ganoderma lucidum The effect of increasing polysaccharide yield

Inactive Publication Date: 2009-10-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cost of carbon source in this conventional medium is high, and the microfactors are lacking

Method used

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  • Novel culture medium formulation of glossy ganoderma mycelium fermentation and optimization method
  • Novel culture medium formulation of glossy ganoderma mycelium fermentation and optimization method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1). The Ganoderma lucidum strain code-named "Ganoderma lucidum" is selected, which is preserved by the Zhejiang Academy of Forestry Sciences.

[0034] 2). Determine the most suitable formula for conventional culture medium (Formula 1)

[0035] On the basis of previous experiments, glucose was selected as the carbon source, soybean meal and yeast extract were selected as the nitrogen source, and the combination of potassium dihydrogen phosphate and magnesium sulfate was used as the analysis factor. Through the orthogonal test of four factors and three levels, the effect of extracellular polysaccharides was observed. Yield, the conventional medium formula selection.

[0036] The fermentation conditions are: the inoculum amount is 10%, the liquid amount is 80ml in a 250ml Erlenmeyer flask, and it is cultured for 7 days in a constant temperature shaker with a rotation speed of 180r / min and a temperature of 28°C, and the pH is natural.

[0037] The extraction method of polysacch...

Embodiment 2

[0061] 1). Select the Ganoderma lucidum (Ganoderma lucidum) code-named "Chi Zhi No. 18", which is preserved by Zhejiang Academy of Forestry Sciences.

[0062] 2). Determine the most suitable formula for conventional culture medium (Formula 1)

[0063] On the basis of previous experiments, we selected glucose as the carbon source, soybean meal powder and yeast extract as the nitrogen source, and potassium dihydrogen phosphate and magnesium sulfate as inorganic salts. Observe the production of extracellular polysaccharides through orthogonal experiments with four factors and three levels. , Carry out the regular medium formula selection.

[0064] The fermentation conditions are: the inoculum amount is 10%, the liquid amount is 80ml in a 250ml Erlenmeyer flask, and it is cultured for 7 days in a constant temperature shaker with a rotation speed of 180r / min and a temperature of 28°C, and the pH is natural.

[0065] The extraction method of polysaccharides is as follows: after fermenta...

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Abstract

The invention relates to bio-pharmaceutical production technology, in particular to a novel culture medium formulation of glossy ganoderma mycelium fermentation and an optimization method. According to the mass percent, the formulation comprises the components: 0.8-2.0% of tartary buckwheat, 0.5-2.5% of coix seed, 0.5-2.0% of glucose, 0.5-3.0% of soybean flour, 0.1-0.5% of yeast extract, 0.01-0.2% of monopotassium phosphate, 0.01-0.1% of magnesium sulfate and 98.08-89.70% of water; the optimization method is operated according to the following sequence: glossy ganoderma strain is determined; the formulation 1 which is most suitable for the conventional culture medium is determined; the optimal formulation 2 of buckwheat culture medium is determined; the optimal formulation 3 of coix seed culture medium is determined; the optimal formulation 4 of culture medium which is formed by simultaneously adding the buckwheat and the coix seed in the formulation 1 is determined; the optimized final formulation 5 is obtained. The invention aims at providing the novel culture medium formulation of glossy ganoderma polysaccharide fermentation and the optimization method which have low cost, rich micro-factors and reasonable trophic structure, and can effectively increase the yield of glossy ganoderma polysaccharide fermentation.

Description

Technical field [0001] The invention relates to a new medium formula and optimization method for the fermentation of Ganoderma lucidum mycelium. It belongs to the field of biopharmaceutical production technology. Background technique [0002] Fungi have a long history as medicine in our country. It is recorded in the earliest medicine book "Shen Nong's Materia Medica" and many other herbal medicines since then, such as Ganoderma lucidum, Poria, Cordyceps, etc., which are still widely used. A large number of medicinal fungi are rich in a variety of medicinal or health-care functional components, such as polysaccharides, triterpenoids, glycopeptides, etc., which play a role in improving human immunity, suppressing tumors, treating cardiovascular diseases, anti-virus, etc. Important biological activity. [0003] Ganoderma lucidum is a precious traditional medicinal fungus. It has multiple functions such as two-way immune regulation, lowering blood lipids, anti-aging and anti-tumor. ...

Claims

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Application Information

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IPC IPC(8): C05G1/00C05F11/00
Inventor 李卫旗
Owner ZHEJIANG UNIV
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