291 results about "Yeast cell extract" patented technology

The invention discloses a method for improving the fermentation performance of clostridium aerovorans and application of the clostridium aerovorans, and according to the method, an effective amount of methionine or a derivative thereof is added into a fermentation culture medium of the clostridium aerovorans to completely replace expensive complex organic nitrogen sources such as yeast extracts. The method successfully solves the three technical bottlenecks of slow biomass accumulation, low ethanol yield and dependence on expensive nitrogen sources in the fermentation process of the clostridium aerovorans by utilizing synthesis gas or CO2 / H2 mixed gas. Experiments prove that methionine or a derivative thereof can synergistically promote growth metabolism of thalli and a synthesis path of a product, so that the gas conversion efficiency of a carbon source is remarkably improved and metabolism is guided to flow to ethanol synthesis while the growth rate and the final biomass of the thalli are improved, and thus the production capacity of alcohols is synchronously enhanced. The method is simple in process and remarkable in effect, and an efficient and economical new strategy is provided for reducing the production cost of biofuel and promoting industrial application of a synthesis gas biorefinery technology.

The invention relates to the technical field of microbial fermentation, in particular to a fermentation medium for producing oxytetracycline. According to the fermentation culture medium, a corn starch-molasses-wheat hydrolyzed sugar composite carbon source, a soybean cake powder-corn steep liquor-yeast extract composite nitrogen source and a choline chloride-betaine-L-lysine precursor accelerant in a specific proportion are used as a core innovative system, and a form regulating agent, a defoaming agent and inorganic salt are cooperatively added. Through the synergistic effect of multiple components, the fermentation titer and yield of oxytetracycline are remarkably improved, the fermentation period is shortened, the generation of heteroacid is effectively inhibited, the mycelium form is optimized, and the oxytetracycline fermentation medium is suitable for industrial large-scale production of oxytetracycline.

The invention discloses a fermentation process for shortening a fermentation period and efficiently synthesizing Ectoine. The fermentation process comprises the following steps: screening and activating high-yield strains, preparing a fermentation culture medium, performing fermentation culture, performing sectional dissolved oxygen regulation and control, performing double-stage pH dynamic regulation and control, performing carbon-nitrogen ratio dynamic regulation based on metabolic flow analysis, performing osmotic pressure gradient induction in the later stage of fermentation, and extracting and purifying a product. According to the fermentation process for shortening the fermentation period and efficiently synthesizing the Ectoin, the expression quantity of key enzymes for synthesizing the Ectoin is increased by 80% or above compared with that of an original strain through the Halomonassp.ECT-09 strain bred through ultraviolet-nitrosoguanidine compound mutation, the basic yield reaches 35 g / L, a foundation is laid for high yield, and the method is suitable for industrial production. A glucose-trehalose mixed carbon source and a yeast extract-ammonium nitrate mixed nitrogen source are adopted, and an L-aspartic acid-L-asparagine mixed precursor is added, so that the metabolic pathway is shortened, and the loss of an intermediate product is reduced.

The invention relates to the technical field of skin care product raw materials, and particularly discloses a composition for controlling oil and removing acne and application thereof. The composition for controlling oil and removing acne comprises the following components in parts by mass: 3.2478-5.413 parts of a humectant; 3-5 parts of a penetration enhancer; 0.0072 to 0.012 part of a preservative; 3.1164 to 5.194 parts of a composite extract product; 2.6286 to 4.381 parts of a solvent; the composite extract is prepared by compounding a lotus corniculatus seed extract, a yeast extract, a rosa multiflora flower extract and a parsley flower extract. The acne-removing mask has the advantage of further improving the acne-removing effect.

The invention discloses a culture medium for identifying gardnerella vaginalis, and relates to the technical field of bacterial culture media, the key points of the technical scheme are as follows: the culture medium comprises the following raw material components by concentration: 10.0 g / L of casein pancreatin digest, 3.0 g / L of cardiac pancreatin digest, 5.0 g / L of yeast extract powder, 1.0 g / L of corn starch, 5.0 g / L of sodium chloride and 13.0 g / L of agar, and the final concentration of defiberized sheep blood is 5%. The pharmaceutical composition is prepared from 1 mg / mL of sodium alginate, 1 mg / mL of sodium alginate, 1 mg / mL of polyanethole Sulfonate (SPS) and 10 [mu] g / mL of vancomycin. According to the invention, through optimal combination of specific components and dosages thereof, rapid identification and screening of gardnerella vaginalis can be realized, and the method has the advantages of accurate result, strong specificity and the like, and has practical popularization and application values.

This invention discloses a processing technology for antibody-enhancing probiotic feed, which includes raw material pretreatment, substrate preparation, sterilization, cooling, strain activation, aseptic inoculation, constant-temperature anaerobic fermentation, immune-enhancing compounding, filtration and homogenization, and aseptic filling and storage. The fermentation substrate liquid includes purified water, molasses, yeast extract, compound trace elements, and buffer salts. A compound microbial community composed of *Lactobacillus plantarum*, *Bacillus subtilis*, *Bifidobacterium*, and yeast is used for fermentation. The temperature is controlled at 35–39°C during fermentation, and intermittent stirring is employed. After fermentation, astragalus polysaccharide, echinacea extract, and trehalose are added for compounding. This invention reduces the loss of active ingredients by adding immune-enhancing components after fermentation, and improves the survival rate of probiotics and fermentation stability through intermittent stirring. The resulting liquid feed has a high live bacteria content and immune-enhancing effect.

The invention discloses a fish hydrolyzed collagen peptide-based flavor beverage and a preparation method thereof, and relates to the technical field of food beverages. The flavored beverage comprises the following raw materials: fish hydrolyzed collagen peptide, a compound flavor modification stabilizer, a compound sweetening agent, concentrated fruit and vegetable juice, casein phosphopeptides, VC magnesium phosphate, zinc lactate, a low-nucleic-acid yeast extract, sodium hyaluronate, an acidity regulator and purified water. The compound flavor modification stabilizer is matched with the low nucleic acid yeast extract to form synergistic flavor regulation and control, the hydrophobic bitter peptide fragment of the fish hydrolyzed collagen peptide and the fishy smell characteristic group are wrapped from the molecular level, and meanwhile, the soft modification on the sensory level is realized through the gamma-aminobutyric acid; the effect of debitterizing and deodorizing without adding artificial essence and a large amount of cane sugar is achieved, the product flavor is natural and harmonious, the sour and sweet taste is palatable, the beverage completely conforms to the current healthy consumption trend of low sugar and no artificial addition, and the problem that the beverage has flavor defects is solved.

The preparation method comprises the following steps: carrying out fishy smell removal treatment on a beef offal raw material, and carrying out enzymolysis on the treated low-fishy-smell beef offal to obtain beef offal enzymatic hydrolysate; chopping the mixed fresh food, adding vegetable oil, heating and frying to obtain fresh food residues and fresh food oil; taking beef offal enzymatic hydrolysate, mixed spices, soy sauce, yeast extract, yolk powder, fresh seasoning oil, fresh residues, raw tallow, amino acid, reducing sugar, edible salt, white granulated sugar, monosodium glutamate and water; the preparation method comprises the following steps: uniformly mixing all the raw materials, carrying out thermal reaction, cooling, discharging, sieving to obtain reaction feed liquid, and concentrating the feed liquid until the water activity is less than or equal to 0.7. The beef offal flavor seasoning paste with natural and harmonious flavor is prepared by combining enzymolysis, Maillard reaction, low-temperature concentration and other technologies while the fishy smell removal efficiency is remarkably improved by adopting a triple fishy smell removal technology of enzymolysis, ultrasonic cleaning and fresh raw material synergistic treatment.

The application provides a sheep fattening complete granular feed and a preparation method and a feeding method thereof, relates to the technical field of feed, and the sheep fattening complete granular feed comprises the following components: ginseng stem and leaf, red yeast extract and sheep group basic feeding daily ration. The application solves the technical problems of high total ginsenoside extraction cost, low single stomach animal utilization rate, slow growth during the sheep fattening period and frequent outbreaks of diseases, achieves the technical effects of improving ginsenoside utilization efficiency, synergistically improving sheep immunity and improving the average daily weight gain of sheep during the whole period, and meanwhile, the process is simple, and the cost is low.

The invention relates to the technical field of microorganisms, and discloses efficient detoxification of a strain of pseudoxanthomonas to ochratoxin A and application of the strain of pseudoxanthomonas to the ochratoxin A. A strain of OTA efficient detoxification strain pseudoxanthomonas X-22 is screened out, and the pseudoxanthomonas X-22 can reduce 50 mu g / mL of OTA (10000 times of the national standard) to 0 within 70 min. The strain is mainly used for performing biological detoxification on OTA through intracellular enzyme, and a detoxification product is OTalpha with extremely low toxicity. The optimal growth conditions of the pseudoxanthomonas X-22 are as follows: 1.5 wt% of glucose is added into a basic culture medium as a carbon source, 1.5 wt% of yeast extract is added into the basic culture medium as a nitrogen source, the pH value of the culture medium is adjusted to 7.5, and the culture temperature is 37 DEG C. The OTA detoxification strain pseudoxanthomonas X-22 is applied to fermentation detoxification of polluted corn and oat, the detoxification rates of the corn and the oat are 61.29% and 97.26% respectively, OTA detoxification strain resources are enriched, and a theoretical basis is provided for application of a biological detoxification technology in the corn and the oat.

This invention discloses a culture medium for the mass sporulation of *Substantia macrantha* and its application. The culture medium comprises the following components: lactose 21-23 g / L, yeast extract 14-16 g / L, potassium chloride 0.4-0.6 g / L, magnesium sulfate 0.4-0.6 g / L, potassium dihydrogen phosphate 0.4-0.6 g / L, disodium hydrogen phosphate 0.55-0.75 g / L, sodium molybdate dihydrate 5-7 mg / L, vitamin B6 50-60 mg / L, arginine 20-30 mg / L, and agar 18-22 g / L, diluted to volume with double-distilled water, with a natural pH. Using the culture medium prepared according to this invention to culture *Substantia macrantha* significantly increases the spore yield (164-fold), reaching 2.21 × 10⁻⁶. 8 conidia / cm 2 This provides a reference for the mass production of the fungal spores.

The invention relates to the technical field of Escherichia coli detection, and particularly discloses a semisolid culture medium for rapid detection of Escherichia coli and a detection method and application thereof, the semisolid culture medium comprises the following components by weight: 4.2-5.8 g / L of agar; 8.5 to 11.5 g / L of tryptone; the semi-solid culture medium comprises the following components: 3.2 to 4.8 g / L of beef extract, 6.5 to 9.5 g / L of lactose, 1.2 to 2.2 g / L of D-glucose, 4.2 to 5.8 g / L of sodium chloride, 42000 to 58000 [mu] g / L of penicillin, 0.12 to 0.28 g / L of cholate, 0.06 to 0.14 g / L of disodium EDTA (Ethylene Diamine Tetraacetic Acid), 0.12 to 0.22 g / L of L-cysteine, 1.2 to 2.8 g / L of yeast extract, 0.005 to 0.015 g / L of vitamin B and an acid-base indicator, the semisolid culture medium and the detection method thereof provided by the invention not only solve the core pain point of the existing escherichia coli detection method in the technical level, but also reduce the operation cost and improve the benefit for enterprises in the economic level, promote the standardization and high efficiency of the food microorganism detection technology in the industry level, and have practicability and industrial popularization value.

The invention discloses a composite microbial agent for degrading micro-plastics in seawater and a preparation method thereof, the microbial agent is composed of effective viable bacteria and auxiliary components, the effective viable bacteria are marine-derived halomonas, acinetobacter and bacillus and pseudomonas with high affinity with the micro-plastics, the effective viable bacteria are compounded according to a specific viable bacteria number ratio, and the microplastics are degraded by the auxiliary components. And function collaboration is realized. The auxiliary components comprise a protective agent consisting of trehalose, skimmed milk, glycerol and cane sugar, and a nutritional agent consisting of glucose and yeast extract. The preparation method comprises the following steps: respectively fermenting, centrifugally concentrating, compounding in proportion, mixing with auxiliary components, and finally drying to prepare powder. The invention further discloses application of the microbial inoculum to degradation of microplastics in beach intertidal zones and ship wastewater systems. The microbial agent is high in pertinence, good in stability in a high-salt seawater environment and high in degradation efficiency, and an effective solution is provided for in-situ bioremediation of marine micro-plastic pollution.

The application discloses a novel plant protein meat strip and a preparation method thereof, and belongs to the technical field of food processing. The plant protein meat strip is prepared from the following raw materials in parts by weight: soybean protein isolate 30-40 parts, pea protein 20-25 parts, gluten powder 10-15 parts, lentinus edodes powder 8-12 parts, konjac powder 5-8 parts, corn starch 10-15 parts, sunflower seed oil 5-8 parts, yeast extract 2-3 parts, carrot powder 2-4 parts, celery powder 1-2 parts, salt 0.8-1.2 parts, ginger powder 0.5-1 part and garlic powder 0.5-1 part. The preparation method comprises the following steps: mixing ingredients, multi-section temperature control double-screw extrusion molding, low-temperature marinating using specific plant-based broth, drying and high-temperature sterilization. Through protein compounding and process optimization, the application solves the problems of poor texture and single flavor of the existing plant meat strips, the product has a taste close to real meat, the flavor is natural and mellow, and the product is suitable for industrial production.

The application provides a yeast mannose oligosaccharide and a preparation method and application thereof. The preparation method comprises the following steps: S1, mixing yeast cell walls with water to obtain a mixed solution; S2, adjusting the pH of the mixed solution to 4-9, performing first enzymolysis on the mixed solution by using a protease to form a first enzymolysis product; secondly, performing second enzymolysis on the first enzymolysis product by using a polysaccharide enzyme to obtain a second enzymolysis product; the amount of the protease is greater than or equal to 0.2% of the weight of the yeast cell walls, and the amount of the polysaccharide enzyme is greater than or equal to 0.1% of the weight of the yeast cell walls; S3, centrifuging the second enzymolysis product to obtain a supernatant and a centrifugal heavy phase; filtering the supernatant by using an ultrafiltration membrane, and the retentate is the yeast mannose oligosaccharide, the permeate is a yeast extract, and the centrifugal heavy phase is a yeast glucan. The yeast mannose oligosaccharide obtained by the application has high purity and high yield, the mannose oligosaccharide is clean, can be fermented by beneficial bacteria, and can be applied as a prebiotic in food.

The invention discloses an amino acid facial cleanser composition and application thereof, and aims to overcome the defects that the existing acne-removing facial cleanser is high in irritation, acne removal and mild cleaning are difficult to consider, and a single component only acts on a single target spot of acne. According to the composition, 5.0-25.0 wt% of sodium cocoyl glycinate, 0.1-2.0 wt% of capryloyl glycine, 0.2-5.0 wt% of nicotinamide and 0.01-0.5 wt% of a hydrolysis yeast extract are taken as core active components, the four components are specifically proportioned to form a synergistic effect, oil control and acne removal are realized through a closed-loop passage of pore cleaning, source oil control and targeted bacteriostasis, and the mild property is guaranteed by amino acid surface activity, and the skin barrier is not damaged. The composition disclosed by the invention can be prepared into facial cleanser and facial mousse, and is excellent in cleaning power and foamability, good in use experience and high in industrialization value.

The purpose of the present invention is to provide a food-or-beverage product composition that contains a yeast material (in particular, a residue during the production of a yeast extract). The food-or-beverage product composition: contains the yeast material exclusively as the main raw material; is easy to produce; and is capable of imparting a cacao or cocoa flavor to food-or-beverage products, is capable of enhancing the cacao or cocoa flavor of food-or-beverage products, or is capable of being used as a substitute material for cocoa mass.　The present invention provides: a food-or-beverage product composition that contains a yeast material, that has a dietary fiber content of at least 35 mass% and a glutamic acid content of less than 1 mass%, and that is characterized in that the yeast material contains 4-hydroxy-2,5-dimethyl-3(2H)-furanone at an internal standard ratio of at least 2.0 when 5-nonanone is used as an internal standard; and a method for producing the food-or-beverage product composition.

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-10 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.21-5.70 times.

To provide a masking agent for off-flavors and off-odors derived from soy protein that can reduce off-flavors and off-odors derived from soy protein, a method for masking off-flavors and off-odors derived from soy protein, and a method for producing soy protein processed foods. [Solution] A masking agent for off-flavors and off-odors derived from soy protein, which contains yeast extract and fermented flavor liquid.

The invention relates to a propionibacterium acnes selective culture medium and a preparation method thereof, the culture medium comprises casein pancreatin digest, meat and stomach enzyme digest, heart and pancreatin digest, yeast extract powder, corn starch, sodium chloride, agar and defiberized sheep blood, and the volume is fixed with distilled water. Compared with an existing culture medium, the culture medium has the advantages of being reliable in detection and high in propionibacterium acnes separation rate and has a good application prospect.

The invention discloses a pineapple wine mixed fermentation process which comprises the following steps: S1, raw material pretreatment: peeling and juicing pineapples, adjusting the sugar degree of fruit juice to 18-22 degrees Brix, adding potassium metabisulfite until the mass concentration is 120-160mg / L, then adding pectinase with the concentration of 100-140mg / L, and standing for 4-8h; s2, strain preparation: performing slant activation culture and seed solution preparation on saccharomyces cerevisiae to obtain a saccharomyces cerevisiae solution with the concentration of 106-108 CFU / mL; the method comprises the following steps: carrying out slant activation culture and seed solution preparation on hansenula polymorpha to obtain a hansenula polymorpha solution with the concentration of 106-108 CFU / mL; the pretreated pineapple juice is inoculated with the two kinds of prepared yeast liquid at the same time according to the proportion, fermentation is conducted at the temperature of 20-24 DEG C, SO2 is added to terminate fermentation after fermentation is finished, filtering and tank reversing are conducted, the pineapple wine is obtained, and the inoculation proportion of the saccharomyces cerevisiae to hansenula polymorpha with grape juice is 1: 10-10: 1. The saccharomyces cerevisiae and the non-saccharomyces cerevisiae are applied to pineapple wine fermentation, so that the quality of the pineapple wine is effectively improved.

The invention discloses a preparation method of a biomass-based slow-release agent for repairing polycyclic aromatic hydrocarbon polluted underground water, which comprises the following steps: 1, preparation of soybean milk fermentation liquor: weighing soybeans, soaking the soybeans in ultrapure water according to a solid-to-liquid ratio of 1: 20-1: 50 for 12-48 hours, pulping, and adding ultrapure water until the volume is 500mL to obtain a soybean milk matrix; inoculating 10mL of polycyclic aromatic hydrocarbon polluted underground water indigenous microorganism bacterial liquid into the soybean milk matrix, and carrying out closed fermentation under a constant-temperature oscillation condition, 2, preparation of a multifunctional composite slow-release agent: weighing 35-60 parts by weight of FeSO4. 7H2O, 1-5 parts by weight of rhamnolipid, 5-10 parts by weight of humic acid, 1-5 parts by weight of methionine, 5-10 parts by weight of peanut peptide, 1-10 parts by weight of soybean lecithin, 3-10 parts by weight of a yeast extract and 40-60 parts by weight of the soybean milk fermentation broth prepared in the step 1; the method has the beneficial effects that the degradation efficiency on organic pollutants such as polycyclic aromatic hydrocarbon is remarkably enhanced, and a reliable material basis and theoretical support are provided for engineering application of an underground water bioremediation technology.

The invention relates to the field of yeast extracts, in particular to a yeast extract containing arginine as well as a preparation method and application of the yeast extract. Wherein the content of arginine in the yeast extract containing arginine is greater than or equal to 6%, and the preparation method comprises the following steps: step 1, culturing a strain to obtain yeast milk; 2, extracting the yeast milk obtained in the step 1, and performing solid-liquid separation to obtain supernate; step 3, performing enzymolysis on the supernate obtained in the step 2, and performing solid-liquid separation to obtain a yeast extract containing arginine; wherein in the step 3, the enzyme used for enzymolysis comprises one or more than two selected from a substance group consisting of papain, alkaline protease and neutral protease. By adopting the preparation method disclosed by the invention, the arginine in the yeast can be more effectively enriched and can be up to 7.35% at most, and the method can be widely applied to the fields of preparing flavor modifiers of foods or human nutrition supplements and the like.

The invention belongs to the technical field of microorganisms, and discloses a high-density fermentation process of vagina-sourced lactobacillus crispatus. The invention discloses a fermentation medium which comprises glucose, tryptone, beef extract, yeast extract, dipotassium phosphate, ammonium citrate, L-cysteine or salt thereof, magnesium sulfate and manganese sulfate. According to the fermentation culture medium provided by the invention, through scientific compounding of a carbon source, a nitrogen source and key factors, the final viable concentration and thallus activity of vagina-derived lactobacillus crispatus can be remarkably improved. The fermentation culture medium is adaptive to the micro-aerobic metabolism characteristics of vagina-derived lactobacillus crispatus by precisely regulating and controlling the dissolved oxygen level (initial dissolved oxygen is controlled to be 5% or below), and when the fermentation culture medium is used for culturing lactobacillus crispatus, the fermentation delay period can be remarkably shortened to 2-3 h, the final viable bacterium yield is greatly increased to 1 * 10 < 10 > CFU / mL or above, and the production cost is reduced. And an efficient and stable technical support is provided for industrial production of the probiotic preparation.

The invention relates to the technical field of seasonings, and particularly discloses a composite sausage seasoning and a preparation method thereof. The seasoning is prepared from edible salt, isomaltooligosacharide, sodium glutamate, a yeast extract, shiitake mushroom powder, spices, red yeast rice powder, a rosemary extract, nisin, trehalose, curdlan, fermented celery powder, epsilon-polylysine and the like according to a specific ratio. The preparation method comprises the steps of basic raw material mixing, microencapsulation slow-release treatment, stage mixing, spice adding and the like, curdlan is used for embedding the rosemary extract, the slow-release effect is achieved, and a vacuum mixing technology is adopted for ensuring uniformity. The seasoning provided by the invention has synergistic antibacterial and antioxidant properties, can significantly prolong the shelf life of sausages, enhance flavor level and umami strength, maintain salty taste perception while reducing the sodium content, improve the product texture and processing stability, and meet the production requirements of natural and healthy meat products.

[Problem] Yeast extract is a natural product, and therefore often has had a problem in stability. Under these circumstances, the present invention addresses the problem of providing a composition in which the stability and clarity of yeast extract are secured. [Solution] The present inventors have made extensive studies. As a result, it has been found that the problem can be solved by dissolving yeast extract, the yeast extract being extracted from yeast cells with an organic solvent such as ethanol and containing a yeast-derived phospholipid or the like, in a specific solvent. This finding has led to the accomplishment of the present invention.

The invention belongs to the technical field of food processing, and particularly relates to a multi-source protein extract composite flavoring base material as well as preparation and application thereof. The multi-source protein extract composite flavoring base material contains 30-60 parts by weight of a yeast extract, 8-25 parts by weight of a plant peptide composition, glucose and a vegetable extract. By combining various plant peptides with the yeast extract and matching with the natural vegetable extract, not only are the defects of a single raw material in taste, aroma and color overcome, but also the contents of umami amino acids and sweet amino acids in the multi-source protein extract composite flavor base material are increased, the content of bitter amino acids is reduced, and the taste of the multi-source protein extract composite flavor base material is improved. The bad flavor caused by multiple proteins is improved.

The present application relates to the technical field of seasoning package, in particular to a water-soluble plant source preservative seasoning package and a preparation method thereof, the seasoning package raw materials include drinking water, brewing soy sauce, brewing vinegar, edible salt, pine mushroom seasoning, ginger, cumin, Sichuan pepper, star anise, cassia bark, yeast extract and water-soluble polysaccharide of Inonotus obliquus, the water-soluble polysaccharide of Inonotus obliquus and the water-soluble active ingredients of ginger, cumin, Sichuan pepper, star anise and cassia bark form a synergistic antibacterial system to achieve preservation; in the present application, by introducing water-soluble polysaccharide of Inonotus obliquus, a synergistic antibacterial system is constructed with the water-soluble active ingredients of ginger, cumin, Sichuan pepper, star anise and cassia bark, and on the premise of not adding chemical preservatives, synthetic flavor enhancers and artificial additives throughout the process, the broad-spectrum antibacterial effect of the standard chemical preservatives is realized.

This invention proposes a Bacillus compound inoculant, its preparation method, and its application, relating to the field of microbial technology. The method of this invention includes the following steps: selecting 18%-25% glucose, 18%-25% yeast extract, 0.3%-0.7% manganese sulfate, 1%-3% potassium dihydrogen phosphate, 1%-3% diammonium citrate, 3%-7% sodium acetate, and 0.1%-0.5% arginine by mass fraction, with the remainder being distilled water, and thoroughly mixing them to prepare a fermentation medium; selecting Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, and Bacillus lentigines, and obtaining a seed culture for inoculation at a constant temperature; mixing the various seed cultures in a ratio of 0.1-0.5:0.1-0.5:95-98:0.1-2:0.1-2 to prepare a compound inoculant, and fermenting to obtain the Bacillus compound inoculant. The compound microbial agent prepared by this invention can not only change the soil nutrient structure and the distribution of microbial communities in the soil, but also change the content of active ingredients in the rhizome of Ligusticum chuanxiong, thereby significantly promoting the growth of Ligusticum chuanxiong rhizome.

The invention discloses a rosa roxburghii tratt fruit composite beverage with a hangover alleviating effect and a preparation method and application thereof, and belongs to the technical field of biology. The roxburgh rose beverage disclosed by the invention comprises the following raw materials in parts by weight on the basis of 100 parts by weight: 8-16 parts of roxburgh rose concentrated juice, 5-15 parts of crystalline fructose, 0.8-2.5 parts of soybean peptide powder, 0.8-1.8 parts of yeast extract and the balance of water. Experiments prove that the formula product has the advantages of moderate taste, aromatic flavor, good stability and the like, and can keep stable taste and flavor during long-term storage. Animal experiments verify that the roxburgh rose beverage has an obvious hangover alleviating effect by improving ALDH activity, accelerating ethanol metabolism and reducing blood ethanol mass concentration, can improve liver injury caused by drinking by increasing liver SOD, GSH-Px, CAT activity and GSH content, reducing MDA content, enhancing body antioxidant ability and inhibiting lipid peroxidation reaction, and has a liver protection effect.