821 results about "Yeast cell extract" patented technology

The invention relates to a concrete anti-cracking self-repairing agent in the technical field of building materials, and aims to solve the problem that concrete cracks are difficult to self-repair. According to the technical scheme, the self-repairing agent is characterized by comprising a microbial repairing unit formed by ZSM-5 type zeolite molecular sieve loaded bacillus pasteurii spores, mesoporous silica particles loaded with L-calcium aspartate and Fe3O4 (at) SiO2 core-shell nanoparticles which are fixed through an in-situ polymerization coating layer to form a magnetic thermal response capsule layer coating the surface of the microbial repairing unit, pLGA microspheres containing yeast extract are positioned on an interface of zeolite and hydrogel, and a trehalose-anhydrous calcium chloride eutectic compound is used as a directional mineralization accelerant. The concrete anti-cracking self-repairing agent provided by the invention is mainly used for intelligent identification and self-repairing of concrete cracks, and efficient repairing is realized through a microbial mineralization, calcium ion slow release and magnetic thermal response synergistic mechanism.

The invention relates to the technical field of environmental restoration, in particular to a slow-release material for synergistically restoring heavy metal and organic compound contaminated soil based on modified biochar and microorganisms and an application method thereof.The slow-release material is prepared from, by weight, 50-80 parts of modified biochar, 30-40 parts of sodium alginate, 10-20 parts of gelatin, 5-10 parts of glycerin and 3-5 parts of calcium carbonate. The multi-modified biochar is prepared from, by weight, 300-400 parts of a functional flora mixed solution and 5-10 parts of mixed nutrient elements of glucose, yeast extract and KHPO, and through the synergistic effect of the multi-modified biochar and the functional flora, efficient immobilization (the immobilization rate gt is 60%) of heavy metal (lead, cadmium and chromium) and efficient degradation (the degradation rate gt is 70%) of organic pollutants (polycyclic aromatic hydrocarbon and petroleum hydrocarbon) are achieved.

The invention relates to a functional microorganism-containing organic-inorganic compound fertilizer and a production method thereof, and relates to the technical field of fertilizers, the functional microorganism-containing organic-inorganic compound fertilizer comprises an inorganic core layer, an organic coating layer and a functional microorganism outer layer, the functional microorganism outer layer comprises the following components in parts by mass: 10-15 parts of compound functional microorganisms, 5-10 parts of synergistic growth-promoting microorganisms, 2-3 parts of trehalose, 1-2 parts of mannitol, 10-15 parts of sodium alginate, 8-10 parts of chitosan, 2-4 parts of a yeast extract and 30-40 parts of a modified activated carbon carrier. The production method comprises the following steps: preparing an inorganic core layer, preheating the inorganic core layer, and spraying and coating an organic coating layer material to obtain a primary product with an organic coating layer; and coating the surface of the organic coating layer of the primary product with a functional microorganism outer layer material, drying at low temperature, and screening to obtain the organic-inorganic compound fertilizer containing the functional microorganisms. The fertilizer has the effect of improving the slow release effect of the fertilizer and the survival rate of microorganisms.

The invention relates to the field of nutrient solutions, and particularly discloses a high-calcium-zinc dietary nutrient solution for promoting skeletal development and a preparation method thereof, the high-calcium-zinc dietary nutrient solution for promoting skeletal development comprises the following raw materials: calcium citrate, zinc citrate, a zinc-containing yeast extract, vitamin D3, a nutrition enhancer, functional oligosaccharide, xanthan gum, anhydrous citric acid, essence, a sweetening agent and water; the preparation method comprises the following steps: mixing the zinc citrate, the zinc-containing yeast extract, the seaweed meal, the natto powder, the stachyose and the trehalose to prepare a mixture A; uniformly premixing stevioside, xanthan gum and trehalose to obtain a mixture B; s4, vitamin D3 powder and trehalose are premixed and dissolved, and a mixture C is prepared; heating the residual water, adding the mixture A, the fructo-oligosaccharide, the anhydrous citric acid, the calcium citrate, the mixture B, the trehalose and the mixture C, adding the essence, and stirring to obtain the beverage. The preparation method has the characteristic of preparing the liquid calcium-zinc dietary nutrient solution with good absorption and utilization rate and palatability.

The application relates to an organic-inorganic compound fertilizer containing functional microorganisms and a production method thereof, and relates to the technical field of fertilizers. The fertilizer comprises an inorganic core layer, an organic coating layer and a functional microorganism outer layer. The functional microorganism outer layer comprises the following components in mass fractions: 10-15 parts of compound functional microorganisms, 5-10 parts of synergistic growth-promoting microorganisms, 2-3 parts of trehalose, 1-2 parts of mannitol, 10-15 parts of sodium alginate, 8-10 parts of chitosan, 2-4 parts of yeast extract and 30-40 parts of modified active carbon carriers. The production method comprises the following steps: preparing the inorganic core layer, preheating the inorganic core layer, spraying the organic coating layer material to coat the surface, and obtaining a preliminary product with the organic coating layer; coating the functional microorganism outer layer material on the surface of the organic coating layer of the preliminary product, low-temperature drying and screening, and obtaining the organic-inorganic compound fertilizer containing the functional microorganisms. The application has the effects of improving the slow-release effect of the fertilizer and the survival rate of the microorganisms.

The invention relates to the field of food and health care products, and discloses a composition for dispelling effects of alcohol and protecting liver, which comprises 20-100 parts of ampelopsis grossedentata leaf extract; 50 to 200 parts of a radix puerariae extract; 10-50 parts of a semen hoveniae extract; 10-50 parts of a turmeric extract; 10 to 100 parts of a yeast extract; 5-50 parts of a licorice extract; and 5 to 20 parts of pyrroloquinoline quinone disodium salt. The ampelopsis grossedentata leaf extract, the kudzuvine root extract and the hovenia dulcis thunb extract are utilized, alcohol decomposition is promoted, the blood ethanol concentration is reduced, and drunkenness symptoms are relieved. Puerarin improves activity of liver metabolic enzymes, accelerates discharge of ethanol and acetaldehyde, and reduces liver damage. The anti-oxidation effects of dihydromyricetin and PQQ can eliminate alcohol metabolism free radicals and protect liver cells. The components such as puerarin, hovenia dulcis thunb, curcumin and glycyrrhizic acid jointly promote liver cell regeneration, inflammation and oxidative damage are reduced, the levels of ALT and AST in serum are reduced, and liver health is maintained.

The invention belongs to the field of analytical chemistry and mathematical modeling, and particularly relates to a method for predicting and identifying whether soy sauce contains yeast extracts, which comprises the following steps: 1) taking different types of soy sauce as modeling samples; 2) carrying out decolorization pretreatment on the modeling sample; (3) carrying out spectrum scanning on the modeling sample by adopting a Raman spectrum technology to obtain original Raman spectrum information; 4) preprocessing the Raman spectrum data obtained in the step 3); 5) sampling for multiple times to establish a sparse logistic regression model, and recording important wave numbers; 6) counting wavenumber importance; 7) selecting an important wave number; 8) using TabPFN to establish a prediction model; (9) predicting and judging whether the yeast extract is added into the soy sauce sample or not by utilizing the established prediction model; compared with the prior art, the method can quickly and accurately identify whether the soy sauce such as light soy sauce and dark soy sauce contains the yeast extract or not, and is simple and quick in identification process, short in analysis time, high in accuracy and high in practicability.

The invention belongs to the technical field of functional foods and health foods, and particularly relates to an anti-saccharification and anti-aging aronia melanocarpa fruit composition and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 150 to 400 parts of aronia melanocarpa fruit fermentation liquor, 10 to 80 parts of lycium ruthenicum, 10 to 80 parts of ginseng, 0.5 to 2.5 parts of grape seed extract, 10 to 70 parts of collagen peptide, 0.5 to 4 parts of sodium hyaluronate, 0.1 to 0.8 part of resveratrol and 0.03 to 0.3 part of yeast extract. The content of type I collagen peptide in the skin is increased, the generation of in-vitro anti-glycation end products is inhibited, and the anti-glycation and anti-aging effects are achieved.

The invention relates to the field of seasonings, in particular to a preparation method of a seasoning. The method comprises the following steps: crushing and screening rice to obtain rice powder, and crushing edible salt to obtain edible salt powder; crushing and screening the dried mushrooms to obtain mushroom powder, and crushing and screening the dried fruits and vegetables to obtain fruit and vegetable powder; conveying the wheat enzymolysis leavening, the wheat protein zymolyte, the rice powder, the edible salt powder, the mushroom powder, the fruit and vegetable powder, the tricholoma matsutake extract and the yeast extract to a mixing machine, uniformly mixing, granulating by a granulating machine, drying and sterilizing to obtain the tricholoma matsutake seasoning. Furthermore, the mixing state of the raw materials is monitored and judged in real time in an intelligent mode in the preparation process of the seasoning, so that the uniform mixing operation is timely decided and controlled, and the mixing uniformity of the raw materials is ensured.

The invention provides schizochytrium limacinum FJ-1 and application thereof in production of phospholipid type EPA (eicosapentaenoic acid). The application comprises the following steps: taking a cryopreserved schizochytrium limacinum strain, and inoculating the schizochytrium limacinum strain into a liquid seed culture medium for pre-culture; the method comprises the following steps: inoculating pre-cultured schizochytrium limacinum into a fermentation tank according to the inoculum size of 5-10% v / v, culturing for 48-60 hours at the sea salt concentration of 0.5-1% w / v, fermenting for 48-72 hours, supplementing sea salt to a culture medium in batches until the sea salt concentration is 3-5% w / v, and continuously culturing for 96-120 hours by controlling the glucose concentration and the yeast extract concentration through supplementing materials. The technical problem that the synthesis efficiency of schizochytrium limacinum phospholipid type EPA is low is solved through precise regulation and control of salinity stress, an efficient, economical and generalizable innovative scheme is provided for industrial production of functional lipid, and the method is suitable for large-scale production.

The invention relates to the technical field of bacterium enrichment nutrient solutions, in particular to a bacterium enrichment nutrient solution for fermentation and a preparation method of the bacterium enrichment nutrient solution. The culture medium is prepared from the following raw materials in parts by weight: maltodextrin, crystalline glucose, low-pigment yeast extract powder, refined soy peptone, dipotassium phosphate, monopotassium phosphate, magnesium sulfate heptahydrate, sodium chloride, phytic acid, L-ascorbic acid, manganese sulfate monohydrate, biotin, thiamine hydrochloride and choline chloride. Through a double-carbon-source structure, maltodextrin and crystalline glucose cooperate, metabolic oscillation is avoided, and the energy utilization rate is increased; the low-pigment yeast extract powder and the refined soy peptone are complementary, so that the rapid growth requirement of thalli is met; phytic acid is chelated with metal ions to prevent precipitation, L-ascorbic acid is used for resisting oxidation, and biotin and thiamine activate a coenzyme pathway to realize accurate regulation and control of a metabolic network.

An object of the present invention is to provide a lactase solution showing good permeation through a filtration filter, a lactase solution showing good permeation through a filtration filter and having a good residual lactase activity, and a lactase solution showing good permeation through a filtration filter and having a good residual lactase activity even when a lactase solution having a low protease activity and a low arylsulfatase activity as contaminant enzymes is used.A lactase solution containing any of (i) to (iii) below.(i) 0.0001 to 0.1 mass % of an unsaturated fatty acid or a salt thereof(ii) 0.0001 to 0.1 mass % of a saturated fatty acid salt(iii) 0.01 to 10 mass % of at least one selected from yeast extract, soybean peptone, pea protein, a casein degradation product, and corn steep liquor

The invention discloses a culture medium for increasing the yield of paracoccus astaxanthin and application of the culture medium. The culture medium comprises the following components according to concentration: 4-20g / L of yeast extract; 8-20 g / L of beef extract; 4 to 20 g / L of bacteriological peptone; 10 to 30 g / L of glucose; the concentration of KH2PO4 is 0.1 to 0.2 g / L; the concentration of Na2HPO4. 12H2O is 0.1 to 0.5 g / L; 0.1 to 1 g / L of MgSO4. 7H2O; 0.1 to 1 g / L of FeSO4. 7H2O; the concentration of CaCl2. 2H2O is 0.02 to 0.1 g / L; the pH is 7.2 + / -0.2. The yeast extract, the beef extract and the bacteriological peptone are selected and mixed to serve as a nitrogen source, accumulation of paracoccus biomass and astaxanthin can be promoted synergistically, and meanwhile the problem of precipitation after the culture medium is sterilized is solved.

The invention provides an in-vitro cell-free protein synthesis system for inserting non-natural amino acid. The reaction system comprises: (1) a yeast cell extract; (2) non-natural amino acids; (3) an exogenous orthogonal aminoacyl tRNA synthetase / orthogonal tRNA pair; (4) a template containing a target protein gene sequence, wherein a codon for coding at least one amino acid in the target protein gene sequence is mutated into a termination codon; by adopting the reaction system, the insertion efficiency of the non-natural amino acid and the expression quantity of the non-natural amino acid protein can be improved.

The invention relates to the technical field of food, and particularly provides a preparation method and application of an oyster sauce based on Sanzan gum. The Sanzan gum is used as a raw material for preparing the oyster sauce for the first time, the preparation method of the oyster sauce is optimized, the problems of poor stability and high cost of the oyster sauce can be effectively solved, and meanwhile, the texture characteristics of the oyster sauce are improved. More specifically, in the raw materials of the oyster sauce, the flavoring substances comprise oyster juice, edible salt, white granulated sugar, a yeast extract and sodium glutamate; in the stabilizer of the oyster sauce, the ratio of hydroxypropyl distarch phosphate to Sanzan gum is (1-8): (0.1-0.4). The Sanzan gum endows the oyster sauce with relatively good firmness, viscosity, cohesiveness, viscosity index and taste; moreover, the shelf life is prolonged while the addition amount of the Sanzan glue is small, and the production cost of the oyster sauce can be effectively reduced.

The invention belongs to the technical field of edible flavors and fragrances, and particularly relates to a method for preparing bidirectional controlled-release meat flavor by using bean dreg protein. The method comprises the following steps: taking bean dreg protein as a raw material, mixing bean dreg protein zymolyte, yeast extract, amino acid or peptide, reducing sugar and grease, carrying out three-stage heating, and respectively mixing the three-stage heating products according to a specific ratio. The obtained essence has the characteristic of bidirectional controlled-release fragrance, is pure, strong, mellow and vivid in meat flavor, can be well applied to food seasonings, and has a wide application prospect.

The invention discloses an improved self-induction culture medium and a culture process, and belongs to the technical field of bioengineering. The improved self-induction culture medium is prepared from 10 g / L of glucose, 3-5 g / L of glycerin, 3 g / L of lactose, 24 g / L of yeast extract, 8 g / L of casein hydrolysate, 0.02-0.05 mM of malonic acid, 1 mM of methionine and 1-3 mM of MgSO4, a phosphate buffer solution is added to control the pH to be 7.2, and the improved self-induction culture medium is prepared by mixing after sterilization. Cells are inoculated to an improved self-induction culture medium for culture, automatic switching of growth-induction stages can be realized based on a pH and DO linkage dynamic feeding control method, the operation complexity is reduced, and the controllability is higher. The culture medium does not need a special inducer, cost is reduced, and protein expression quantity is improved.

To provide a topical skin preparation that effectively promotes collagen production within the skin, exhibits excellent usability, and effectively enhances post-use stratum corneum moisture content.SOLUTION: A topical skin preparation comprises niacinamide, a heparin-like substance, dipotassium glycyrrhizinate, a collagen peptide, a yeast extract, and glycylglycine. It is preferable that the topical skin preparation further contains hydrogenated soybean phospholipids and phytosterols. It is more preferable that the topical skin preparation has a niacinamide content of 5 mass% or more. It is also preferable that the topical skin preparation further contains at least one plant extract selected from Arnica montana and Plantago major.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of bioengineering, and particularly relates to kluyveromyces marxianus engineering bacteria for synthesizing eicosapentaenoic acid and application of the kluyveromyces marxianus engineering bacteria. According to the engineering bacterium, pyruvate decarboxylase PDC1 genes, alcohol dehydrogenase ADH1 genes and alcohol dehydrogenase ADH2 genes are sequentially knocked out from a kluyveromyces marxianus FIM1 delta ura3 strain; meanwhile, a citrate lyase ACL gene, a pyruvate oxidase PO gene and a phosphate transacetylase PTA gene are inserted into the loci respectively, so that synthesis of acetyl coenzyme A is enhanced; then a plurality of genes are sequentially inserted into a Kluyveromyces marxianus genome, and then a vector of a delta 8 desaturase delta 8D gene is transferred into the Kluyveromyces marxianus genome. The invention also provides a method for efficiently preparing the EPA, namely the Kluyveromyces marxianus engineering strain is used as a fermentation strain, glucose, a yeast extract, peptone and other components are used as a culture medium, and the yield of the EPA produced by culturing and fermenting reaches 8.71 mg / g of dry cell weight.

The invention relates to the technical field of agricultural waste recycling and feed industry, in particular to a traditional Chinese medicine residue feed additive as well as a preparation method and application thereof. The preparation method comprises the following steps: drying traditional Chinese medicine residues, crushing to obtain a crushed material, mixing the crushed material with a compound enzyme, carrying out enzymolysis to obtain an enzymolysis material, mixing the enzymolysis material with lactobacillus plantarum LP-01, carrying out anaerobic fermentation to obtain a fermented material, mixing the fermented material with a yeast extract and manganese sulfate to obtain a mixture, mixing the mixture with water, extracting, filtering to obtain a filtrate, concentrating, and carrying out freeze drying to obtain a finished product. The traditional Chinese medicine residue feed additive is obtained. By adopting the traditional Chinese medicine residue feed additive provided by the invention, the diarrhea rate of weaned piglets can be reduced, the stress resistance of aquatic animals is improved, and the growth performance of poultry is improved.

The invention provides a preparation method of composite degradable TIEL aerogel, and relates to the technical field of production of TIEL aerogel. The preparation method of the composite degradable TIEL aerogel comprises the following steps: S1, firstly, preparing a culture medium, and dissolving 20g / L of glucose, 5g / L of yeast extract, 5g / L of peptone, 2.7 g / L of Na2.HPO4, 0.5 g / L of MgSO4. 7H2O and 1.5 g / L of citric acid in deionized water; s2, adjusting the pH value of the culture medium with HCl, then performing high-temperature sterilization treatment on the culture medium, cooling the culture medium to room temperature, inoculating pre-cultured acetobacter aceti into the culture medium, performing static culture for 7 days at 30 DEG C, then repeatedly cleaning the harvested film with deionized water, and removing impurities with an alkaline solution to obtain a fresh bacterial cellulose film. According to the cellulose aerogel, chitosan is introduced as a natural electron donor, so that the triboelectric property of the cellulose aerogel is optimized, and meanwhile, the degradability is kept.

This invention provides a novel method for preparing a medical collagen sponge, relating to the field of medical sponge technology. The invention uses yeast extract obtained by Soxhlet extraction of brewer's yeast as a raw material, which is mixed and reacted with polyvinyl alcohol and graphene oxide to obtain the novel medical collagen sponge. The medical collagen sponge prepared by this invention exhibits excellent performance and good hemostatic effect. Furthermore, the preparation method is simple, and it degrades postoperatively, demonstrating significant application value in the field of medical materials.

The invention discloses a method for improving the fermentation performance of clostridium aerovorans and application of the clostridium aerovorans, and according to the method, an effective amount of methionine or a derivative thereof is added into a fermentation culture medium of the clostridium aerovorans to completely replace expensive complex organic nitrogen sources such as yeast extracts. The method successfully solves the three technical bottlenecks of slow biomass accumulation, low ethanol yield and dependence on expensive nitrogen sources in the fermentation process of the clostridium aerovorans by utilizing synthesis gas or CO2 / H2 mixed gas. Experiments prove that methionine or a derivative thereof can synergistically promote growth metabolism of thalli and a synthesis path of a product, so that the gas conversion efficiency of a carbon source is remarkably improved and metabolism is guided to flow to ethanol synthesis while the growth rate and the final biomass of the thalli are improved, and thus the production capacity of alcohols is synchronously enhanced. The method is simple in process and remarkable in effect, and an efficient and economical new strategy is provided for reducing the production cost of biofuel and promoting industrial application of a synthesis gas biorefinery technology.

The invention relates to the technical field of pet food, in particular to flavoring agent powder for pet dogs and a preparation method of the flavoring agent powder. Comprising the following raw materials: 40 parts of beef liver, 10 parts of hairtail, 7-40 parts of ricefield eel, 1 part of protease, 0.5-1 part of lipase, 0.5-1 part of flavor enzyme, 5-10 parts of saccharides, 10 parts of fish oil, 5-10 parts of maltodextrin, 0.5-1 part of sodium hydroxide, 5 parts of phosphoric acid, 3 parts of amino acid, 2 parts of thiamine, 1 part of yeast extract and 0.5-1 part of lactoferrin. The dog feed can increase the appetite of dogs from the sense of smell, maintain the health balance of intestinal flora of dogs and improve the micro-ecological environment of intestinal tracts.

The invention relates to the technical field of microbial fermentation, in particular to a fermentation medium for producing oxytetracycline. According to the fermentation culture medium, a corn starch-molasses-wheat hydrolyzed sugar composite carbon source, a soybean cake powder-corn steep liquor-yeast extract composite nitrogen source and a choline chloride-betaine-L-lysine precursor accelerant in a specific proportion are used as a core innovative system, and a form regulating agent, a defoaming agent and inorganic salt are cooperatively added. Through the synergistic effect of multiple components, the fermentation titer and yield of oxytetracycline are remarkably improved, the fermentation period is shortened, the generation of heteroacid is effectively inhibited, the mycelium form is optimized, and the oxytetracycline fermentation medium is suitable for industrial large-scale production of oxytetracycline.

The present invention relates to the field of biotechnology, and particularly to a preparation method of yeast extract rich in betaine. The present invention provides a preparation method of yeast extract rich in betaine. Among them, the yeast extract rich in betaine includes protein, amino acids, peptides, nucleotides, and betaine. The mass percentage content of protein is 50% - 75%, and the mass percentage content of betaine is 0.2% - 4%. In the present invention, yeast rich in betaine is obtained by the yeast absorbing and enriching betaine in the betaine-rich carbon source during the growth process, and then the yeast extract rich in betaine is obtained through enzymatic hydrolysis and refinement. This technology makes up for the blank of yeast extract of betaine, and is mainly involved in the fields of food, health food, feed, medicine, etc.

The invention provides a fermentation culture medium for increasing the content of surfactin, application of the fermentation culture medium and a fermentation method. The fermentation culture medium is used for fermenting bacillus subtilis; the fermentation culture medium comprises the following substances in percentage by weight: 4-7% of a carbon source; 0.65-2.25% of a nitrogen source; 0.13 to 0.6 percent of phosphate; 0.015 to 0.05 percent of magnesium sulfate; 0.0007 to 0.02% of calcium chloride; 0.0005 to 0.001% of manganese sulfate; 0.0006 to 0.06 percent of ferric sulfate; 0.5-1% of amino acid; 0.5-3% of soybean fiber and the balance of water; wherein the pH value of the fermentation culture medium is 6.5-7; the inoculum size of the bacillus subtilis liquid in the fermentation culture medium is 3-5% by volume fraction; wherein the carbon source is selected from at least one of molasses, glucose, brown sugar, maltodextrin and starch; the nitrogen source comprises a combination of urea and at least one of corn steep liquor dry powder, yeast extract and yeast powder.

The invention relates to the technical field of animal feed, in particular to a high-efficiency growth-promoting pig compound additive and a preparation method thereof. The high-efficiency growth-promoting pig compound additive is prepared from the following raw materials in parts by weight: 5-8 parts of bacillus subtilis; 3 to 5 parts of bacillus licheniformis; 4 to 6 parts of fructo-oligosaccharide; 3 to 5 parts of galactooligosaccharide; 8 to 12 parts of yeast extract; 2-4 parts of a yucca extract; 1 to 2 parts of cellulase; 1-2 parts of acid protease; 0.5 to 1.5 parts of curcumin; 0.3 to 0.8 part of tea polyphenol; 1-3 parts of taurine; and 2 to 4 parts of glycine chelated zinc. According to the efficient growth-promoting pig compound additive and the preparation method thereof, multi-dimensional collaborative optimization is adopted, probiotics and prebiotics are combined, colonization of beneficial bacteria in intestinal tracts is promoted, metabolic active substances are generated, antibiotic dependence is replaced, organic microelements are used for replacing high-dose inorganic zinc, and the risk of environment residues is reduced; the natural anti-inflammatory component and the light stabilizer reduce oxidative stress and photosensitive toxicity, are significantly superior to the traditional scheme, and have both environmental friendliness and cost effectiveness.

The invention relates to the technical field of blood culture bottles, in particular to a composition and application thereof in preparation of a microbial culture medium. Yeast extract powder, soy peptone and casein tryptone are combined into a nitrogen source, and an aerobic and anaerobic culture medium for clinical pathogenic microorganism culture is formed. Through comparison verification, in growth experiments of nine aerobic bacteria and seven anaerobic bacteria, the culture speed and the biomass accumulation of the aerobic and anaerobic culture media added with the composition are integrally superior to those of other components excluded in the prior art and screening. Therefore, the composition can effectively promote the performance of a blood culture product, improve the clinical blood flow infection detection capability, inhibit antibiotic abuse and save the life of a patient.