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Phlebopus portentosus cultivation method

A technology for cultivating dark brown net stalks and strains, which can be used in botanical equipment and methods, fertilizer mixtures, gardening, etc., and can solve the problems of inconvenient application, easy to bring other miscellaneous bacteria, unstable inoculation effect, etc., and achieve the same size. , golden color, simple nutrition effect

Active Publication Date: 2009-07-29
YUNNAN INST OF TROPICAL CROPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mycelium, mycelium, fruiting bodies, spores and other natural strains of Dictyostelium phelicus distributed in the soil, due to the limitation of seasonal conditions, it is impossible to find them all the year round, it is not convenient for application, and it is easy to carry other species. Miscellaneous bacteria, the inoculation effect is unstable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A, cultivating Dictyostelium ducas sporocarpus tissue piece with female seed culture medium to prepare female seed, the preparation of female seed medium is formulated by following raw material and method:

[0040] Raw material: 200.0g potato, 20.0g glucose, 2.0g yeast extract, MgSO 4 1.0g, KH 2 PO 4 1.0g, 15.0g agar, 1000ml water

[0041] Preparation:

[0042] a1) Wash the fresh potatoes, peel them, weigh them, then cut them into thin slices, put them into a large beaker, add a certain amount of water, boil for 20 minutes and filter to get the filtrate, add glucose, agar, yeast extract, MgSO 4 、KH 2 PO 4 , boil until the agar is completely melted, and the pH value is about 6.0, to obtain the mother seed medium; divide the medium into Erlenmeyer flasks, sterilize at 120°C for 30 minutes, and pour it into a petri dish in an ultra-clean workbench after cooling. use;

[0043] a2) Aseptically operate in an ultra-clean workbench, separate the wild fruiting body tissue...

Embodiment 2

[0054] A, cultivating Dictyostelium bruceae sporocarp tissue block with strain culture medium to prepare female species, the preparation of female species medium is formulated by following raw materials and methods:

[0055] Raw material: 150.0g potato, 15.0g glucose, 1.0g yeast extract, MgSO 4 2.0g, KH 2 PO 4 2.0g, 20.0g agar, 1000ml water, pH value about 5.0;

[0056] The following steps are the same as in Example 1.

[0057] B, ferment and cultivate the liquid bacterial classification with liquid culture medium, the raw material composition of liquid culture medium is as follows: potato 150.0g, sucrose 15.0g, yeast extract 1.0g, MgSO 4 2.0g, KH 2 PO 4 2.0g, water 1000ml, pH value about 5.0;

[0058] The following steps are the same as in Example 1.

[0059] C, cultivate solid bacterial classification with solid medium, the raw material composition of solid medium is as follows: Miscellaneous wood sawdust 10.0kg, grain 4.0kg, MgSO 4 15.0g, KH 2 PO 4 15.0g, pH value...

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Abstract

The invention relates to a method for culturing a mother spawn, a liquid spawn and a solid spawn of phlebopus portentosus. The method is characterized in that: raw materials such as potato, sucrose, glucose, agar, yeast extract, MgSO4, KH2PO4, rubber wood timber dust or other weed tree dust, grains and water are used to prepare culture media of the mother spawn, the liquid spawn and the solid spawn according to a certain proportion; and according to the culture method from the mother spawn, the liquid spawn to the solid spawn, the phlebopus portentosus mother spawn and liquid spawn are firstly cultured, and then the liquid spawn is inoculated on the solid spawn culture medium so as to culture the solid spawn. The culture mediums are particularly suitable for the culture of the phlebopus portentosus mother spawn, the liquid spawn and the solid spawn. The method can be used to carry out the rapid culture of the phlebopus portentosus spawns from the mother spawn, the liquid spawn to the solid spawn.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for cultivating Dictyostelium pheasatum boletus strains. Background technique [0002] Phlebopus portentosus (Phlebopus portentosus) is a delicious edible fungus with rich nutritional value and is loved by consumers in the place of origin. At present, it cannot be artificially cultivated. All collected from wild natural growth. [0003] Most boletus are mycorrhizal edible fungi, and the current research on the cultivation of mycorrhizal edible fungi is mostly semi-artificial simulation cultivation and artificial promotion of bacteria ponds. Semi-artificial simulated cultivation needs to inoculate artificially isolated pure culture strains or natural strains on the roots of corresponding saplings for "mycorrhizalization", and then cultivate "mycorrhized" seedlings to cultivate fruiting bodies to grow. Due to the limitation of seasonal conditions, the mycelium, mycelia, frui...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G3/00
Inventor 纪开萍何明霞王文兵张春霞刘静曹旸
Owner YUNNAN INST OF TROPICAL CROPS
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