Phlebopus portentosus cultivation method

A dark brown reticulum and strain culture technology, applied in the direction of botany equipment and methods, fungi, fertilizer mixture, etc., can solve the problems of inconvenient application, easy to bring other miscellaneous bacteria, unstable inoculation effect, etc. Consistent, golden color, simple nutrition results

Active Publication Date: 2010-11-03
YUNNAN INST OF TROPICAL CROPS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mycelium, mycelium, fruiting bodies, spores and other natural strains of Dictyostelium phelicus distributed in the soil, due to the limitation of seasonal conditions, it is impossible to find them all the year round, it is not convenient for application, and it is easy to carry other species. Miscellaneous bacteria, the inoculation effect is unstable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] a. Use the mother seed culture medium to cultivate the fruit body tissue blocks of Boletus edulis dark brown to prepare the mother seed. The mother seed culture medium is prepared by the following raw materials and methods:

[0040] Raw materials: 200.0g potato, 20.0g glucose, 2.0g yeast extract, MgSO 4 1.0g, KH 2 PO 4 1.0g, 15.0g agar, 1000ml water

[0041] Preparation:

[0042] a1) Wash the fresh potatoes, peel them, weigh them, and cut them into thin slices, put them in a large beaker, add a certain amount of water, boil for 20 minutes, filter the filtrate, add glucose, agar, yeast extract, and MgSO 4 , KH 2 PO 4 , Boil until the agar is completely melted, and the pH value is about 6.0, to obtain the mother seed culture medium; divide the culture medium into Erlenmeyer flasks, sterilize at 120°C for 30 minutes, and pour it into a petri dish in the ultra-clean workbench after cooling. use;

[0043] a2) Use a scalpel to separate the wild fruit body tissue pieces of Boletus edu...

Embodiment 2

[0054] a. Use the culture medium to cultivate the fruit body tissue block of Boletus edulis dark brown to prepare the mother seed. The preparation of the mother seed medium is prepared by the following raw materials and methods:

[0055] Ingredients: potato 150.0g, glucose 15.0g, yeast extract 1.0g, MgSO 4 2.0g, KH 2 PO 4 2.0g, agar 20.0g, water 1000ml, pH value is about 5.0;

[0056] The following steps are the same as in Example 1.

[0057] b. Use liquid culture medium to ferment and cultivate liquid strains. The raw material composition of the liquid culture medium is as follows: potato 150.0g, sucrose 15.0g, yeast extract 1.0g, MgSO 4 2.0g, KH 2 PO 4 2.0g, 1000ml water, pH value about 5.0;

[0058] The following steps are the same as in Example 1.

[0059] c. Use solid culture medium to cultivate solid strains. The raw material composition of the solid culture medium is as follows: 10.0kg miscellaneous wood sawdust, 4.0kg grain, MgSO 4 15.0g, KH 2 PO 4 15.0g, pH value is about 5.0; ...

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PUM

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Abstract

The invention relates to a method for culturing a mother spawn, a liquid spawn and a solid spawn of phlebopus portentosus. The method is characterized in that: raw materials such as potato, sucrose, glucose, agar, yeast extract, MgSO4, KH2PO4, rubber wood timber dust or other weed tree dust, grains and water are used to prepare culture media of the mother spawn, the liquid spawn and the solid spawn according to a certain proportion; and according to the culture method from the mother spawn, the liquid spawn to the solid spawn, the phlebopus portentosus mother spawn and liquid spawn are firstly cultured, and then the liquid spawn is inoculated on the solid spawn culture medium so as to culture the solid spawn. The culture mediums are particularly suitable for the culture of the phlebopus portentosus mother spawn, the liquid spawn and the solid spawn. The method can be used to carry out the rapid culture of the phlebopus portentosus spawns from the mother spawn, the liquid spawn to the solid spawn.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to a method for cultivating a strain of Boletus edulis dark brown. Background technique [0002] Phlebopus portentosus (Phlebopus portentosus) is a kind of delicious edible fungus with delicious taste and rich nutritional value, which is deeply loved by consumers in the producing area. It is still not artificially cultivated. The fruiting bodies of Phlebopus portentosus are sold on the market. All are collected from wild natural growth. [0003] Most of the bolete are mycorrhizal edible fungi. At present, most researches on the cultivation of mycorrhizal edible fungi are semi-artificial simulation cultivation and artificial promotion of mushroom ponds. Semi-artificial simulation cultivation requires that artificially isolated pure culture strains or natural strains are inoculated on the roots of the corresponding tree seedlings for "mycorrhiza", and then the "mycorrhiza" seedlings are cultiva...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G1/04C05G3/00
Inventor 纪开萍何明霞王文兵张春霞刘静曹旸
Owner YUNNAN INST OF TROPICAL CROPS
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