Composition of multiple fungus possessing effects of anti tumour and adjusting immunity preparation method, and usage

A composition and application technology, applied in the field of microbial engineering, can solve problems such as hidden safety hazards, ineffective experimental evidence, unclear specific efficacy, etc., and achieve the effects of good safety, improved immunity, and simple and easy methods.

Inactive Publication Date: 2007-05-09
德阳创新生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above products are all prepared by simple proportioning of the finished product of the culture of beneficial bacteria, the specific efficacy is not clear, and there is no experimental evidence to prove its effect
Moreover, the combination of a variety of fungi and medicinal ingredients makes it a safety hazard

Method used

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  • Composition of multiple fungus possessing effects of anti tumour and adjusting immunity preparation method, and usage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation of embodiment one multi-bacteria composition of the present invention

[0020] The preparation method is as follows (see Figure 1 for the flow process):

[0021] 1. Various nutrient elements of the fungus are sterilized and mixed to make a liquid medium. The formula of the medium is (1000ml sterile water): yeast extract 10g, protein 8g, potassium dihydrogen phosphate 0.8g, magnesium sulfate 5g, Green onion sugar 10g, glucose 20g (20g each of corn flour, soybean flour, and potato flour, add 500ml of water and boil for 10 minutes, then take the filtrate).

[0022] 2. Select prepared ganoderma lucidum and agaricus blazei strains to make production seeds respectively, and inoculate the production seeds in the prepared liquid medium at a ratio of 1:1, and the inoculum amount is ≥ 10%.

[0023] 3. After the inoculated culture medium is fully stirred, carry out aseptic aeration culture in a fermenter. The fermentation temperature is controlled between 20°C and...

Embodiment 2

[0025] The preparation of embodiment two multi-bacteria composition of the present invention

[0026] 1. Various nutrient elements of fungi are sterilized and mixed to make a liquid medium. The medium formula is (1000ml sterile water): 10g yeast extract, 9g protein, 0.8g potassium dihydrogen phosphate, 5g magnesium sulfate, green onion Sugar 10g, glucose 20g (20g each of corn flour, soybean flour, and potato flour, add 500ml of water and boil for 10 minutes, then take the filtrate).

[0027] 2. Select the prepared strains of Lentinus edodes, Grifola frondosa and Flammulina velutipes to make production seeds respectively, and inoculate the production seeds in the prepared liquid medium at a ratio of 1:1:1, and the inoculation amount is ≥10%.

[0028] 3. After the inoculated culture medium is fully stirred, carry out aseptic aeration culture in a fermenter. The fermentation temperature is controlled between 20°C and 28°C for fermentation culture, and the fermentation time is not...

Embodiment 3

[0030] The preparation of embodiment three multi-bacteria composition of the present invention

[0031] 1. Various nutrient elements of fungi are sterilized and mixed to make a liquid medium. The medium formula is (1000ml sterile water): 10g yeast extract, 8g protein, 0.8g potassium dihydrogen phosphate, 5g magnesium sulfate, green onion Sugar 10g, glucose 20g (20g each of corn flour, soybean flour, and potato flour, add 500ml of water and boil for 10 minutes, then take the filtrate).

[0032] 2. Select prepared asafoetida, tiger milk mushroom, poplar mushroom, and shimeji mushroom strains to make production seeds respectively, and inoculate the production seeds in the prepared liquid medium in a ratio of 1:1:1:1 Within, the inoculation amount ≥ 10%.

[0033] 3. After the inoculated culture medium is fully stirred, carry out aseptic aeration culture in a fermenter. The fermentation temperature is controlled between 20°C and 28°C for fermentation culture, and the fermentation ...

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Abstract

A multi-fungus composition for improving immunity and preventing and treating cancer is prepared from two or more of 14 edible funguses including champignon, genoderma, Jinzheng mushroom, etc through culturing. Its preparing process is also disclosed.

Description

Technical field: [0001] The invention belongs to the field of microbial engineering, and specifically relates to a multi-bacteria composition with anti-tumor and immunoregulatory effects, and also relates to a preparation method and application of the composition. Background technique [0002] Modern biotechnology research shows that many probiotic fungi have the effect of improving human immune cells and can inhibit tumor cells. In recent years, there have been reports that polysaccharides extracted from shiitake mushrooms are used in the treatment of tumors and Ganoderma lucidum is used in the treatment of tumors (Cao Jing et al., Ganoderma lucidum Dynamic observation of polysaccharide extracts from fermentation broth on cellular immunity of tumor-bearing mice, Chinese Journal of Microecology, 2004, 6(3) 140-143; Song Bingsheng, Yang Yulong, Introduction to the study of antitumor activity of lentinan[J] Chinese Herbal Medicine 29(7) : 492-495; Wang Yun, Yang Jinming, Wang ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074A61P37/02A61P35/00C12N1/14A23L1/30A61K36/06A01G1/04A23L31/00
Inventor 王殿学王晶晶
Owner 德阳创新生物工程有限公司
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