Method for producing high ganoderma triterpenes content ganoderma lucidum mycelium
A triterpene ganoderma lucidum and production method technology, applied in the field of edible and medicinal mushroom production technology, can solve the problems of extensive production technology, low product efficacy, low absorption and utilization rate of pharmacological components, etc., achieve improved production technology, increase the content of functional components, Favorable effect of absorption and utilization
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Embodiment 1
[0042] A method for producing ganoderma lucidum mycelium with high ganoderma triterpenoids, comprising the steps of:
[0043] 1) Cultivation of Ganoderma lucidum strains: the cultivation temperature is 25-26°C,
[0044] Get the ganoderma lucidum strain and cultivate it in the strain culture medium for 20 to 30 days. The formula of the strain culture medium consists of: 200g of potatoes, 20g of glucose, 3g of potassium dihydrogen phosphate, 1.5g of magnesium sulfate heptahydrate, 110mg of vitamin B, agar 18g, water 1000ml;
[0045] 2), shaker seed: keep the temperature at 25-26°C, put the strain culture medium with Ganoderma lucidum strain in the step 1) into the 30L stirring tank, dilute with water, then place it on a shaker, and stir 3 to 4 days, control the stirring speed to 100 to 120 rpm;
[0046] 3), one-level amplification: keep the temperature at 25-26°C, inhale the culture medium diluted and stirred in the step 2) into a 300L seed tank under negative pressure, and cu...
Embodiment 2
[0057] A method for producing ganoderma lucidum mycelium with high ganoderma triterpenoids, comprising the steps of:
[0058] 1) Cultivation of Ganoderma lucidum strains: the cultivation temperature is 25-26°C,
[0059] Get the ganoderma lucidum strain and cultivate it in the strain culture medium for 20 to 30 days. The formula of the strain culture medium consists of: 250 g of potatoes, 25 g of glucose, 4 g of potassium dihydrogen phosphate, 2 g of magnesium sulfate heptahydrate, 120 mg of vitamin B, and 20 g of agar , water 1000ml;
[0060] Step 2)~step 4) are as embodiment 1;
[0061] 5), fermentation culture: keep the temperature at 25-26°C, suck the culture medium cultured in the step 4) into a 7-ton fermenter under negative pressure, and add the fermentation medium to ferment,
[0062] In terms of mass percentage, the formula of the fermentation medium is composed of: 3% corn flour, 4% sucrose, 0.02% potassium dihydrogen phosphate, 0.3% ammonium sulfate, 0.4% yeast pow...
Embodiment 3
[0069] A method for producing ganoderma lucidum mycelium with high ganoderma triterpenoids, comprising the steps of:
[0070] 1) Cultivation of Ganoderma lucidum strains: the cultivation temperature is 25-26°C,
[0071] Get the ganoderma lucidum strain and cultivate it in the strain culture medium for 20 to 30 days. The formula of the strain culture medium is composed of: 225g of potatoes, 22.5g of glucose, 3.5g of potassium dihydrogen phosphate, 1.7g of magnesium sulfate heptahydrate, and 115mg of vitamin B , agar 19g, water 1000ml;
[0072] Step 2)~step 4) are as embodiment 1;
[0073] 5), fermentation culture: keep the temperature at 25-26°C, suck the culture medium cultured in the step 4) into a 7-ton fermenter under negative pressure, and add the fermentation medium to ferment,
[0074] In terms of mass percentage, the formula of the fermentation medium is composed of: 2% corn flour, 3% sucrose, 0.015% potassium dihydrogen phosphate, 0.25% ammonium sulfate, 0.3% yeast p...
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