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Method for producing high ganoderma triterpenes content ganoderma lucidum mycelium

A triterpene ganoderma lucidum and production method technology, applied in the field of edible and medicinal mushroom production technology, can solve the problems of extensive production technology, low product efficacy, low absorption and utilization rate of pharmacological components, etc., achieve improved production technology, increase the content of functional components, Favorable effect of absorption and utilization

Inactive Publication Date: 2015-11-04
JIANGSU ZHONGXIANG HIGH TECH IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are countless types of Ganoderma lucidum medicines, health foods, and foods developed today, but most of them are repetitions and imitations of low-end primary Ganoderma lucidum products. Low, the pharmacological effect is not obvious, the production process is relatively extensive, and the absorption and utilization rate of pharmacological components is not high

Method used

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  • Method for producing high ganoderma triterpenes content ganoderma lucidum mycelium

Examples

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Effect test

Embodiment 1

[0042] A method for producing ganoderma lucidum mycelium with high ganoderma triterpenoids, comprising the steps of:

[0043] 1) Cultivation of Ganoderma lucidum strains: the cultivation temperature is 25-26°C,

[0044] Get the ganoderma lucidum strain and cultivate it in the strain culture medium for 20 to 30 days. The formula of the strain culture medium consists of: 200g of potatoes, 20g of glucose, 3g of potassium dihydrogen phosphate, 1.5g of magnesium sulfate heptahydrate, 110mg of vitamin B, agar 18g, water 1000ml;

[0045] 2), shaker seed: keep the temperature at 25-26°C, put the strain culture medium with Ganoderma lucidum strain in the step 1) into the 30L stirring tank, dilute with water, then place it on a shaker, and stir 3 to 4 days, control the stirring speed to 100 to 120 rpm;

[0046] 3), one-level amplification: keep the temperature at 25-26°C, inhale the culture medium diluted and stirred in the step 2) into a 300L seed tank under negative pressure, and cu...

Embodiment 2

[0057] A method for producing ganoderma lucidum mycelium with high ganoderma triterpenoids, comprising the steps of:

[0058] 1) Cultivation of Ganoderma lucidum strains: the cultivation temperature is 25-26°C,

[0059] Get the ganoderma lucidum strain and cultivate it in the strain culture medium for 20 to 30 days. The formula of the strain culture medium consists of: 250 g of potatoes, 25 g of glucose, 4 g of potassium dihydrogen phosphate, 2 g of magnesium sulfate heptahydrate, 120 mg of vitamin B, and 20 g of agar , water 1000ml;

[0060] Step 2)~step 4) are as embodiment 1;

[0061] 5), fermentation culture: keep the temperature at 25-26°C, suck the culture medium cultured in the step 4) into a 7-ton fermenter under negative pressure, and add the fermentation medium to ferment,

[0062] In terms of mass percentage, the formula of the fermentation medium is composed of: 3% corn flour, 4% sucrose, 0.02% potassium dihydrogen phosphate, 0.3% ammonium sulfate, 0.4% yeast pow...

Embodiment 3

[0069] A method for producing ganoderma lucidum mycelium with high ganoderma triterpenoids, comprising the steps of:

[0070] 1) Cultivation of Ganoderma lucidum strains: the cultivation temperature is 25-26°C,

[0071] Get the ganoderma lucidum strain and cultivate it in the strain culture medium for 20 to 30 days. The formula of the strain culture medium is composed of: 225g of potatoes, 22.5g of glucose, 3.5g of potassium dihydrogen phosphate, 1.7g of magnesium sulfate heptahydrate, and 115mg of vitamin B , agar 19g, water 1000ml;

[0072] Step 2)~step 4) are as embodiment 1;

[0073] 5), fermentation culture: keep the temperature at 25-26°C, suck the culture medium cultured in the step 4) into a 7-ton fermenter under negative pressure, and add the fermentation medium to ferment,

[0074] In terms of mass percentage, the formula of the fermentation medium is composed of: 2% corn flour, 3% sucrose, 0.015% potassium dihydrogen phosphate, 0.25% ammonium sulfate, 0.3% yeast p...

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Abstract

The invention discloses a method for producing high ganoderma triterpenes content ganoderma lucidum mycelium. After strain culture is finished, strains undergo the shaking table culture, primary amplification, secondary amplification and fermentation cultivation process and finally undergo filter pressing and drying smashing to obtain the high ganoderma triterpenes content ganoderma lucidum mycelium. The method has the advantages that a culture medium formula and a fermentation culture medium formula are optimized according to strain design, a production process is improved, and the functional component content of ganoderma triterpenes is greatly improved and can be more than twice that of ordinary ganoderma lucidum. The method for producing the high ganoderma triterpenes content ganoderma lucidum mycelium is performed at 26+ / -1 DEG C from the culture, the whole production process is free of acid-bases and organic solvents, active functional components are protected to the largest extent, and the method is favorable for the absorption and utilization by the human body and can bring the pharmacological effects of ganoderma lucidum into play to the largest extent.

Description

technical field [0001] The invention belongs to the technical field of production technology of edible and medicinal fungi, and in particular relates to a production method of ganoderma lucidum mycelium with high ganoderma triterpenoids. Background technique [0002] Ganoderma lucidum has been hailed as the "auspicious grass" by Chinese medical scientists in the past dynasties. There have been a lot of studies on the pharmacological effects of Ganoderma lucidum. Chemotherapy effect, sedative and tranquilizing effect, cardiotonic and anti-myocardial ischemia effect, blood lipid regulation effect, hypoglycemic effect, anti-asthma effect, liver protection effect, anti-hypoxia and anti-aging effect, etc. With the in-depth study of Ganoderma lucidum by modern science, so far, a total of more than 350 chemical components have been isolated, mainly polysaccharides, peptides, amino acids, terpenes, alkaloids, sterols, vitamins, etc. Although Chinese medicine has a long history, the...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 孙勇
Owner JIANGSU ZHONGXIANG HIGH TECH IND CO LTD
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