Lucid ganoderma fungus purification and rejuvenation cultural method and culture medium thereof
A technique for purifying and rejuvenating ganoderma strains, which is applied in the field of culture methods for purifying and rejuvenating ganoderma lucidum strains and its culture medium, can solve the problems of reduced activity of ganoderma lucidum strains, decreased stress resistance of strains, and insufficient activity, etc., and achieve mycelial growth Fast, white and dense mycelia, good agronomic properties
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[0055] The present invention is further described below in conjunction with specific examples of implementation, the purification and rejuvenation test of three Ganoderma lucidum strains SLZ30, SLZ46, and SLZ44.
[0056] The preparation of ganoderma lucidum strain rejuvenation medium comprises the following steps:
[0057] (1) Formula of purified rejuvenation medium
[0058] Ganoderma lucidum mycelia purification medium, that is, No. 1 medium preparation:
[0059] Select 20 round-bottomed test tubes of 20mm×200mm, all culture materials are selected locally, 200g potatoes, 20g glucose, 15g agar powder, 3g potassium dihydrogen phosphate, 1.5g magnesium sulfate, 10g corn flour, 50g bran, oak bark And / or bark of Qinggang tree in any proportion 100g.
[0060] Ganoderma lucidum strain rejuvenation medium, that is, No. 2 medium preparation:
[0061] Select 20 round-bottomed test tubes of 20mm×200mm, all culture materials are selected locally, 200g potatoes, 20g glucose, 15g agar p...
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