Lucid ganoderma fungus purification and rejuvenation cultural method and culture medium thereof

A technique for purifying and rejuvenating ganoderma strains, which is applied in the field of culture methods for purifying and rejuvenating ganoderma lucidum strains and its culture medium, can solve the problems of reduced activity of ganoderma lucidum strains, decreased stress resistance of strains, and insufficient activity, etc., and achieve mycelial growth Fast, white and dense mycelia, good agronomic properties

Inactive Publication Date: 2016-01-20
SAAS BIOTECH & NUCLEAR TECH RES INST
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  • Description
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AI Technical Summary

Problems solved by technology

[0004] Aiming at problems such as the reduction of the activity of the ganoderma strains caused by the existing traditional ganoderma strain culture substrate, the purpose of the present invention is to provide a new rejuvenation medium for the ganoderma lucidum

Method used

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  • Lucid ganoderma fungus purification and rejuvenation cultural method and culture medium thereof
  • Lucid ganoderma fungus purification and rejuvenation cultural method and culture medium thereof
  • Lucid ganoderma fungus purification and rejuvenation cultural method and culture medium thereof

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Embodiment Construction

[0055] The present invention is further described below in conjunction with specific examples of implementation, the purification and rejuvenation test of three Ganoderma lucidum strains SLZ30, SLZ46, and SLZ44.

[0056] The preparation of ganoderma lucidum strain rejuvenation medium comprises the following steps:

[0057] (1) Formula of purified rejuvenation medium

[0058] Ganoderma lucidum mycelia purification medium, that is, No. 1 medium preparation:

[0059] Select 20 round-bottomed test tubes of 20mm×200mm, all culture materials are selected locally, 200g potatoes, 20g glucose, 15g agar powder, 3g potassium dihydrogen phosphate, 1.5g magnesium sulfate, 10g corn flour, 50g bran, oak bark And / or bark of Qinggang tree in any proportion 100g.

[0060] Ganoderma lucidum strain rejuvenation medium, that is, No. 2 medium preparation:

[0061] Select 20 round-bottomed test tubes of 20mm×200mm, all culture materials are selected locally, 200g potatoes, 20g glucose, 15g agar p...

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Abstract

The invention belongs to the field of fungus cultivation and provides a lucid ganoderma fungus purification and rejuvenation cultural method and a culture medium thereof against the problem that the activity of lucid ganoderma fungi is lowered due to an existing traditional lucid ganoderma fungus culture medium. The cultural method comprises the following steps: 1, preparing the lucid ganoderma fungus rejuvenation culture medium, wherein the lucid ganoderma fungus rejuvenation culture medium is prepared from 150-400 g of potatoes, 10-30 g of glucose, 10-25 g of agar powder, 1-5 g of monopotassium phosphate, 0.5-3 g of magnesium sulfate and 30-70 g of lucid ganoderma sporocarp; 2, culturing lucid ganoderma fungi into hypha, and then inoculating the hypha to the lucid ganoderma fungus rejuvenation culture medium obtained in step 1; 3, conducting culturing according to normal conditions, and obtaining rejuvenation fungi. The hypha obtained through the cultural method is quick in growth, pure white, orderly and dense, and the obtained sporocarp is good in agronomic trait.

Description

technical field [0001] The invention belongs to the field of fungal cultivation, and in particular relates to a method for purifying and rejuvenating ganoderma fungi and a culture medium thereof. Background technique [0002] Ganoderma lucidum (ganodermalucidum) is a treasure in the treasure house of Chinese medicinal materials. It is known as the "immortal grass" and is the source of Chinese medicine. As a clinical raw material of traditional Chinese medicine, Ganoderma lucidum has a medicinal history of more than 3,000 years. Ganoderma lucidum has been artificially cultivated in my country for more than 30 years. The cultivation area is wide and there are many varieties. The quality of Ganoderma lucidum directly affects the quality and curative effect of Chinese medicine products. Modern pharmacological research and clinical efficacy have proved that the medicinal value of Ganoderma lucidum includes anti-tumor and anti-radiation functions, anti-cardiovascular system diseas...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 金鑫黄文丽李小林陈祖琴
Owner SAAS BIOTECH & NUCLEAR TECH RES INST
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