32 results about "Multi gene" patented technology

This invention belongs to the field of biomedical technology, specifically relating to the application of DNA damage response gene-related SNPs in the treatment and prognosis of acute myeloid leukemia (AML). This invention is the first to discover that single nucleotide polymorphisms (SNPs) in the DNA damage response pathway (focusing on three major functional modules: damage sensing, signal transduction, and DNA repair) are closely related to the clinical characteristics, treatment response, and survival prognosis of AML. This lays the foundation for constructing a multi-gene risk model based on the DNA damage response pathway and provides theoretical support for integrating germline SNP maps into precision medicine strategies for AML.

Provided are a prime editing system-based method for achieving inversion editing of a large genomic fragment and a composition used. By means of the efficient multi-gene prime editing protein construct ePPEplus in plants, the inversion of large fragments in plants is achieved by means of designing paired pegRNAs (pegL and pegR), and then by means of processes such as reverse transcription, DNA double-stranded annealing, DNA strand synthesis, and repair. The pegL comprises RTT-L, and the pegR comprises RTT-R. The nucleotide sequence of RTT-L is identical to that of strand A at the right terminus of a double-stranded DNA to be inverted. The nucleotide sequence of RTT-R is identical to that of strand B at the left terminus of said DNA. By means of testing in the monocotyledonous plant wheat and dicotyledonous plants Nicotiana benthamiana and tomato, it is indicated that the method is an effective method for mediating precise inversion of large-fragment DNA in plants.

The application discloses a mutant Cas12i nuclease and application thereof, and belongs to the technical field of gene editing. The Cas mutant protein provided by the application is a mutant protein in which the 7th, 168th, 273rd, 332nd, 478th, 505th and 551st amino acids of SEQ ID NO:1 are all mutated into arginine, and other amino acid sequences remain unchanged. The application improves the editing efficiency by mutating multiple amino acids of the wild-type Cas12i protein, and can be used for multi-gene editing.

The application provides a fat sea base gene and adulteration identification method based on multi-gene joint DNA barcoding, four gene joint barcode combinations are formed by adopting nuclear gene ITS2 and chloroplast genes matK, rbcL and psbA-trnH, at least two pairs of optimized alternative primers for each target gene and fat sea genus specific preliminary screening primer Ster-1 are matched, genus level rapid preliminary screening is realized to exclude non-fat sea genus samples and shorten the identification process, and the efficient amplification success rate of multi-gene fragments is ensured through the flexible use of alternative primers; fat sea and round fat sea, Sterculia and mixed adulterants can be accurately distinguished, the problems that traditional identification methods are subjective and species with close genetic relationship are difficult to distinguish are effectively solved; the method is not limited by sample morphology and fragmentation degree, can be widely applied to the true and false identification, quality control and traceability of traditional Chinese medicinal materials fat sea and its products, and provides technical support for standardizing market order and ensuring the safety and effectiveness of clinical medication.

This invention relates to a multi-gene tandem plant expression vector for improving soybean alkali tolerance, its construction method, and its application, belonging to the field of genetic engineering technology. To address the technical problem that simple superposition of multiple genes in soybean sodium salt tolerance improvement easily leads to pathway interference, increased metabolic burden, and poor growth, making it difficult to systematically improve alkali tolerance, this invention utilizes homologous recombination to express genes from the same metabolic pathway... GmNAC133 , GmC2H2 , GmPET6 By constructing a multi-gene tandem plant expression vector and utilizing a soybean hairy root transformation method based on the RUBY reporter gene, a systematic improvement in soybean alkali tolerance was successfully achieved. This invention is not only applicable to improving soybean alkali tolerance but also provides an efficient and reliable technical approach for breeding other crops that are difficult to genetically transform or require multi-gene synergistic regulation, and has broad prospects for widespread application.

This invention discloses a method for multi-gene aggregation breeding of larch and its application in cultivating new larch germplasm, lines, and varieties with excellent performance in terms of biomass, timber properties, and stress resistance. It includes the following steps: construction of a multi-gene aggregation expression backbone vector suitable for larch genetic transformation; cloning of genes regulating target traits such as biomass, timber properties, and stress resistance; construction of the multi-gene aggregation expression vector; larch genetic transformation, screening, and identification; and phenotypic observation and evaluation of the multi-gene-transferred larch materials. Results show that this method can introduce multiple target genes into larch recipient materials through a single genetic transformation, significantly improving the breeding efficiency of obtaining transgenic larch materials with excellent comprehensive traits. This method is of great value in overcoming the breeding bottlenecks of long larch growth cycles and a lack of precise techniques for synergistic improvement of multiple traits, and in the targeted and efficient cultivation of larch materials with excellent comprehensive traits.

This invention provides a microfluidic chip for the multiplex differentiation and diagnosis of different avian influenza virus subtypes and its application. Specifically, it is a microfluidic chip based on TaqMan probes that can simultaneously and rapidly differentiate and diagnose 25 avian influenza subtypes, enabling high-throughput simultaneous detection of multiple gene targets. This invention utilizes high-throughput microfluidic chip technology to simplify the differential diagnosis procedure for avian influenza virus subtypes, resulting in more accurate and time-saving results. It is more suitable for rapid clinical diagnosis of avian influenza, providing technical support for rapid clinical detection, diagnosis, and prevention of avian influenza, and is of great significance for epidemic prevention and control and safeguarding public health security.

This invention discloses a genetically engineered bacterium producing high levels of L-homoserine, its construction method, and its applications, belonging to the field of biotechnology. The genetically engineered bacterium uses *Escherichia coli* W3110 as the substrate cell. By knocking out the metA, lysA, thrBC, pflB, ldhA, poxB, adhE, iclR, sthA, tdcC, lacI, and ptsG genes, and replacing the promoters of ppc, aspC, and gdhA genes with the inducible strong promoter Ptrc, and by performing site-directed mutagenesis on the thrA and asd genes, and simultaneously overexpressing the thrA*, pnhsd, asd, asd*, and rhtA genes using free plasmids, a recombinant strain HOM20 was constructed. This strain, fermented in a 5 L fermenter for 48 h, achieved an L-homoserine yield of 135.4 g / L and a sugar-acid conversion rate of 44%, significantly superior to existing technologies. This invention achieves directional carbon flow allocation, cofactor balance, feedback inhibition removal, and enhanced product efflux through multi-gene synergistic modification, and has broad prospects for industrial application.

The invention discloses a method for enhancing the synthesis capacity of a yeast double-metabolic-pathway pigment product and application, and belongs to the field of synthetic biology and metabolic engineering. The method comprises the following steps: constructing a saccharomyces cerevisiae engineering strain capable of simultaneously synthesizing violacein and lycopene; constructing a mutant library of the global transcription factor SPT15; transforming the mutant library into the engineering strain, and screening the mutant strain with increased yield based on bacterial colony color visualization. According to the invention, a single global transcription factor SPT15 is modified, so that the cell metabolism is regulated and controlled on the whole, the synergistic interaction of a diheterologous synthesis pathway is realized, and the tedious multi-gene rational modification is avoided. The method is easy and convenient to operate and short in period, and a new strategy is provided for efficient construction of yeast cell factories for high-yield pigment and other double-way products.

This invention belongs to the field of plant metabolic engineering and biosynthesis technology, and relates to a recombinant expression vector, engineered bacteria, and their application and method in constructing cotton plants capable of synthesizing artemisinin. This invention integrates five key genes from the artemisinin metabolic pathway—ADS and CYP71AV1 from *Artemisia annua*, and ADH1, DBR2, and ALDH1 from *Bryum arvense*—into a multi-gene overexpression vector for genetic transformation in cotton. Optimal expression strategies for cotton plants capable of synthesizing artemisinin were screened using quantitative real-time radiometric analysis (RCR) and artemisinin content determination. Compared with wild-type plants lacking artemisinin, cotton leaves overexpressing the ADS, CYP71AV1, ADH1, DBR2, and ALDH1 genes showed an artemisinin content as high as 2.34 μg / g DW.

This invention relates to the fields of molecular biology and forensic identification, specifically to a method for bird species identification based on multi-gene co-amplification and nanopore sequencing. The method involves extracting genomic DNA from avian biological samples; using the extracted DNA as a template, amplification is performed using four independent PCR primer pools that specifically target the avian mitochondrial cytochrome C oxidase subunit I gene, cytochrome B gene, 12S rRNA gene, and 16S rRNA gene; after purification of the amplification products, a nanopore sequencing library is constructed and sequenced; finally, bioinformatics analysis, including read length clustering, draft consensus sequence generation, and polishing, is performed on the raw sequencing data to obtain consensus sequences for each target gene. Species identification is then completed by comparing the sequence with a avian sequence database. This method effectively solves the problem that existing technologies cannot simultaneously meet the practical needs of large-scale, rapid, and high-success-rate identification of avian biological samples.

The present application relates to the technical field of multi-gene detection, and in particular to a set of capture probes for follicular lymphoma multi-gene detection, a kit and a detection analysis method ARID1A, ARID1B, ATM, ATP6V1B2, B2M, BCL10, BCL2, BCL6, BCL7A, BTG1, BTG2, BTK, CARD11, CCND3, CD58, CD70, CD79A, CD79B, CDK4, CDKN2A The capture probe set comprises capture probes for specifically binding to 74 follicular lymphoma related genes, and the capture probe set preferably comprises nucleotides with sequences as shown in SEQ ID NO. 1-937. The probe set of the present application can realize one-time detection of variation in the full exon region of 74 follicular lymphoma related genes in the sample to be detected in the same system, has high specificity, high average sequencing depth and wide coverage area.

This invention relates to the fields of gene editing and animal genetic breeding technology, and discloses a precise editing method for key genes regulating lipid metabolism in cattle and sheep. The method includes: constructing a comprehensive lipid metabolism network map integrating transcriptomic, proteomic, and metabolomic data; using a graph neural network to identify key node genes with high flow weights and their negative feedback pathways; simulating cascade reactions after gene perturbation to assess compensatory effects; screening for multi-gene combinations that do not induce significant compensation and can synergistically regulate fat deposition and muscle growth; designing expression vectors containing multiple guide RNA sequences to achieve simultaneous and precise editing; and obtaining edited individuals and validating the phenotype through embryonic or somatic cell nuclear transfer. This invention, through system-level multi-target synergistic editing, avoids metabolic imbalances, improves editing effectiveness, and promotes muscle development while reducing fat deposition, providing a safe and efficient technical path for precision breeding of cattle and sheep.

This invention discloses a Cas mutant protein and its applications, belonging to the field of gene editing technology. The Cas mutant protein provided by this invention is a mutant protein in which amino acids 168, 273, 332, 478, and 599 of SEQ ID NO:1 are all mutated to arginine, while the other amino acid sequences remain unchanged. This invention improves editing efficiency by mutating multiple amino acids in the wild-type Cas12i protein, and can be used for multi-gene editing.

The present disclosure discloses a new Retron editing system and its application in Corynebacterium glutamicum gene editing, specifically discloses a new Retron editing system and demonstrates its application in Corynebacterium glutamicum gene editing, belonging to the technical field of biotechnology and genetic engineering. The new gene editing system provided by the present disclosure is obtained by modifying the existing Retron editing system, which improves the efficiency and capacity of gene editing. The gene editing system of the present disclosure is applied to the construction of a DNA fragment genomic in situ saturation mutation library, which has high construction efficiency, high mutation site coverage and comprehensive mutation types. This method has broad application prospects in the fields of multi-gene editing regulation, DNA regulatory sequence in situ library construction, protein coding gene in situ library construction, enzyme high-throughput screening and the like.

The invention provides systems and methods for computing interpersonal compatibility using multi-modal data fusion that integrates genomic, immunologic, psychometric, biometric, contextual, and reproductive information. Genomic analysis includes variant calling, polygenic scoring, carrier-status evaluation, and HLA / KIR immune-compatibility modeling. Biometric inputs from wearable devices are processed to determine emotional synchrony and autonomic co-regulation. Psychometric and behavioral data are converted into latent-trait embeddings. A machine-learning fusion engine combines all modality-specific feature vectors to generate unified compatibility embeddings and dual outputs representing soulmate-stage suitability and family-planning compatibility. Additional embodiments include donor and surrogate matching, offspring-risk simulation, embryo-viability prediction, and longitudinal model refinement using real-world relational, biometric, or reproductive outcomes.

This invention discloses the application of a biomarker in assessing the risk of prostate cancer recurrence, belonging to the field of tumor molecular diagnostics and bioinformatics technology. The biomarker includes at least one of the TACR2 and LPIN3 genes, and RCCD1, PRSS27, and NAP1L5, with the core detection focusing on the methylation-transcriptional combined characteristics of the TACR2 gene. This invention obtains patient samples, uses RT-qPCR to detect the expression levels of multiple genes, and methylation-specific PCR to detect the methylation level of the TACR2 promoter region. A risk scoring model is constructed using LASSO regression, and patient prognosis is stratified based on the score. This invention solves the problems of traditional assessment indicators being affected by tumor heterogeneity and having limited predictive accuracy. It is the first to use TACR2-related multi-omics characteristics for prostate cancer recurrence assessment. The multi-gene combined model improves predictive accuracy and can be developed into a standardized testing kit, providing a scientific basis for clinical recurrence risk assessment and personalized treatment, with significant industrial and clinical application value.

This invention belongs to the field of plant genetic engineering technology and discloses a method for constructing transgenic hairy roots with multi-gene co-expression of the tanshinone biosynthesis pathway. The method first constructs a multi-gene expression vector containing the SmCPS1, SmKSL1, and SmCYP76AH1 genes, and then introduces the multi-gene expression vector into Agrobacterium rhizogenes. Subsequently, recombinant Agrobacterium rhizogenes is used to infect tanshinone explants, and transgenic hairy roots are induced after co-culture, sterilization culture, and resistance screening. Positive hairy roots are then identified and their expression analyzed by PCR and qRT-PCR, achieving stable co-expression of key genes in the tanshinone biosynthesis pathway. This invention achieves a single co-transformation of multiple key genes, simplifies the multi-gene transformation process, reduces the risk of gene segregation and expression instability caused by multiple transformations, and can provide stable transgenic materials for research on the tanshinone biosynthesis pathway and the metabolic regulation of active components in tanshinone.

A method and system for identifying individual traits are provided. The method includes: obtaining the individual's complete genetic sequence; providing an associated PGS model using data indicating the complete genetic sequence; using the PGS model to calculate corresponding K polygenic scores for K (K≥1) traits associated with the individual; generating an initial behavioral and physiological report of the individual using the K polygenic scores of the K traits, wherein specific scores are assigned to each trait based on the relevance of each trait to each of more than one predetermined activity domain of the individual; performing AI-based processing of the input data, including personalized data of the individual obtained in real time from more than one genetically unrelated source associated with the trait, to obtain an optimized value for each of the specific scores; and continuously optimizing and updating the data in the initial report in real time based on the optimized values ​​to obtain a personalized report of the individual associated with the more than one predetermined activity domain.

This invention discloses a multi-gene mutation prediction method based on a combination of multi-task and multi-instance learning. Belonging to the fields of digital image analysis, pathology, and machine learning, the specific steps are as follows: Preprocessing existing pathological image data by staining normalization; constructing a feature matrix for each pathological image, and further using a two-layer multi-instance learning method to construct a package for each pathological image; constructing a multi-task deep learning network based on transformer and MobileNet; applying the model to a test set and outputting pathological image analysis results. This invention employs a multi-task deep neural network and applies it to the task of predicting multiple gene mutations based on pathological images. Compared to traditional single-task networks, this invention can simultaneously predict the results of multiple tasks, saving computational resources while improving accuracy; furthermore, combining the transformer module with traditional convolution considers both local and global features.

The application discloses a screening marker recovery technology based on a Cre-LoxP combined Flp-Frt recombination system and application. The Cre-Loxp recombination system comprises a Cre recombinase and a LoxP site, and the LoxP nucleic acid combination comprises LoxP71 and LoxP66 sequences which can be specifically recognized and recombined by the Cre recombinase; similarly, the Flp-Frt recombination system comprises a Flp recombinase and a Frt site, and the Frt site comprises a Frt32 sequence which can be specifically recognized and recombined by the Cre recombinase. Due to the lack of screening markers and inducible promoters in the Schizochytrium, the site-specific recombinase cannot be recovered after being integrated into the genome, and therefore, the application aims to combine the two specific recombinase systems, so that the screening markers and the site-specific recombinase can be recovered unlimited times, and thus, the multi-gene editing of the Schizochytrium can be realized.

The application discloses a primer probe composition, application and dynamic monitoring system for postoperative MRD detection of colorectal cancer based on multi-gene methylation qPCR, relates to the technical field of molecular diagnosis and precise tumor treatment, and is related to three genes of KCNQ5, Septin9 and IKZF1.The dynamic monitoring system comprises a qPCR detection module used for detecting the Ct value of a sample, a data acquisition module used for automatically reading the Ct value of a qPCR instrument, calculating a P value, and analyzing an optimal determination threshold value by using a ROC curve, a data analysis module used for determining MRD positivity, MRD negativity or invalid results, and a clinical decision support module used for making clinical decisions in combination with the results of dynamic monitoring.The application has a sensitivity of 82%, a specificity of 95%, low cost, and can output results in 6-8 hours, covers the whole process, and realizes a closed loop from risk stratification to recurrence intervention.

This invention provides a method for efficient multi-gene editing and CAR site-directed integration based on BaEV envelope glycoprotein truncated viral particles and its application in immune cell engineering. The invention provides an MLV-VLP system utilizing BaEV envelope glycoprotein truncated particles to achieve efficient CRISPR-Cas9 delivery without electroporation or the use of randomly integrating viruses. Combined with adeno-associated virus (AAV6) delivery of HDR templates, it enables efficient site-directed integration of CAR sequences at target sites (such as TRAC, PD1, and B2M) in primary human T cells and NK cells. The prepared CAR-T or CAR-NK cells exhibit excellent cell killing, proliferation, and low exhaustion characteristics in vitro, achieving 100% tumor clearance in an in vivo mouse model. It can simultaneously achieve multi-gene knockout with high efficiency, supporting large-scale allogeneic CAR-T and CAR-NK cell production.

This invention relates to a low-memory multi-genome alignment and structural variation integration method for ultra-large-scale closely related genome sets. It solves the problems of existing multi-sequence / multi-genome alignment methods, which suffer from unacceptable time and space overhead on ultra-long sequences, large errors, and inefficiency in identification and integration. It includes S1, sequence input, output, and center sequence preprocessing; S2, direction determination and anchor point retrieval; S3, main strand construction, loop divide-and-conquer, and banded dynamic programming for fine alignment; and S4, structural difference strand identification, block output, and consistent integration. The advantages of this invention are: it can losslessly preserve and integrate structural variation fragments related to inversions and rearrangements during global alignment, ensuring that the output meets the "column consistency" requirements of downstream analysis while expressing strand direction and rearrangement information, and avoiding the extremely slow or even unworkable problems of traditional multiple merging processes in ultra-large file scenarios.

The application discloses a gene marker combination for evaluating HLH risk and use thereof, and belongs to the technical field of gene detection. The gene marker combination is obtained by whole genome association analysis of whole exome sequencing, covers more extensive genetic information, solves the narrowness of the prior art, and provides more comprehensive analysis of the polygenic heterogeneity of HLH. Moreover, the cumulative effect of alleles is comprehensively considered, the genetic susceptibility characteristics of an individual to HLH can be more accurately reflected, and the sensitivity and specificity of gene detection can be improved.

Disclosed herein are methods and compounds for enhancing gene expression by ACT-UP1 compounds. Such methods and compounds can be used to increase expression of specific genes, many of which are associated with a variety of diseases and disorders.

The application relates to the technical field of stem cell engineering and regenerative medicine, and specifically discloses a stem cell treatment method for treating type 2 diabetes. The method comprises the following steps: S1, extracting placenta-derived mesenchymal stem cells; S2, performing multi-gene modification on the mesenchymal stem cells to obtain gene-modified mesenchymal stem cells; S3, pretreating the gene-modified mesenchymal stem cells with a culture medium; S4, extracting microvesicles from the mesenchymal stem cells; S5, mixing the microvesicles, baicalin, L-arginine and vitamin D in a buffer to form a composite preparation; S6, introducing the composite preparation into a patient's body through abdominal cavity intervention; and S7, performing synergistic treatment by combining oral administration of an SGLT2 inhibitor after infusion, and regularly monitoring islet function indexes. The stem cell treatment method can be used for treating type 2 diabetes, and has the advantages of being targeted to improve the islet microenvironment, promoting the recovery of beta cell function and being durable in action.

The application provides a Pantoea agglomerans KVBP1 strain, which is classified and named as Pantoea agglomerans KVBP1, and is preserved in the China Center for Type Culture Collection with a preservation number of CCTCC NO: M 20252888. The application also provides application of the KVBP1 strain in prevention and treatment of kiwi fruit canker disease and a biocontrol agent for preventing and treating kiwi fruit canker disease. The application has the advantages that a strain with a significant prevention and treatment effect on kiwi fruit canker disease is screened from kiwi fruit plant tissues and rhizosphere soil, is identified as the Pantoea agglomerans KVBP1 through multi-gene sequence analysis, and is verified in function through physiological and biochemical determination and in-vitro and in-vivo prevention and treatment effect tests, thereby providing a new high-quality biocontrol strain resource and technical support for healthy and sustainable development of the kiwi fruit industry.

The application belongs to the technical field of biology, and particularly relates to a bidirectional promoter of Andrographis paniculata and application thereof. The nucleotide sequence of the promoter is shown as SEQ ID NO. 1. The bidirectional promoter of Andrographis paniculata provided in the application contains a natural regulatory element, has high compatibility with a plant genome, can realize precise spatiotemporal regulation of genes, avoid silencing risks, and is compatible with the concept of green breeding. Meanwhile, the bidirectional promoter of Andrographis paniculata can drive the synergistic expression of multiple genes through a single promoter, optimize the stability of a vector and the balance of gene expression, and improve the stacking efficiency of multiple genes.

The invention relates to a method of predicting an outcome of a breast cancer subject, comprising determining or receiving the result of a determination of a gene expression profile comprising the expression levels of four or more genes selected from a first, a second and / or a third gene expression profile, wherein the first gene expression profile comprising one or more immune defense response genes selected from the group consisting of: AIM2, APOBEC3A, CIAO1, DDX58, DHX9, IFI16, IFIH1, IFIT1, IFIT3, LRRFIP1, MYD88, OAS1, TLR8, and ZBP1, and / or the second gene expression profile comprising one or more T-Cell receptor signalling genes selected from the group consisting of: CD2, CD247, CD28, CD3E, CD3G, CD4, CSK, EZR, FYN, LAT, LCK, PAG1, PDE4D, PRKACA, PRKACB, PTPRC, and ZAP70, and / or the third gene expression profile comprising one or more PDE4D7 correlated genes selected from the group consisting of: ABCC5, CUX2, KIAA1549, PDE4D, RAP1GAP2, SLC39A11, TDRD1, and VWA2, said gene expression profile being determined in a biological sample obtained from the subject, determining the prediction of the outcome based on the gene expression profile comprising the expression levels of the four or more genes, wherein said prediction is a favourable risk or a non-favourable risk of breast-cancer related death, loco-regional recurrence and / or distant recurrence.