Non-invasive detection method and kit for early screening bladder cancer by multi-gene combination

Abstract

The invention discloses a non-invasive detection method and a kit for early screening a bladder cancer by multi-gene combination. The combined genes include APC, NID2 and p16, and specific methylationprimers are designed to detect three nucleotide sequences of target gene fragment methylation of the APC, the NID2 and the p16, so that early screening and auxiliary diagnosis of the bladder cancer are achieved. Methylation levels of three genetic markers of the APC, the NID2 and the p16 in urinary sediments are analyzed by a three-channel fluorescent quantitative PCR (polymerase chain reaction)method, and a positive or negative result of a sample is judged according to a reported CT (computed tomography) value. The non-invasive detection method can detect 90% of early bladder cancers, 64% of precancerous adenomas (larger than or equal to 1 centimeter) in preclinical study, specificity reaches 100%, invasiveness is completely omitted, the method only needs to collect urine of 50mL, and acceptability of throngs wearing no symptoms is high.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to a non-invasive detection method for multi-gene joint screening of bladder cancer and a kit thereof, which are suitable for detecting whether there is bladder cancer mutation DNA in urine sedimentation cells. Background technique [0002] Bladder cancer (BC) is one of the most common malignant tumors of the urinary system, accounting for the first place in urinary system tumors, more than 90% of which are transitional cell carcinomas (BTCC), of which 80% are invasive superficial carcinomas, which are cured for the first time The subsequent recurrence rate is 70% to 80%, and its incidence and recurrence rate are increasing year by year. The incidence rate of bladder cancer varies by as much as 10 times among countries in the world. Western Europe and North America are the highest, while Eastern Europe and some Asian countries are relatively low. In my country, the incidence of male b...

AI Technical Summary

Problems solved by technology

The combined detection of several genes is the trend of methylation detection in bladder cancer, but whether these reported methylation markers can be used to improve diagnostic sensitivity and specificity is still a problem (Wang Yongqiang, urine EOMES, PCDH17 and Significance of POU4F2 gene methylation analysis for early diagnosis of bladder urothelial carcinoma), Catto et al. studied the methylation of 12 gene promoter regions, and the detection rate was 86%; Patent CN104141009B multi-target detection method for early bladder cancer , 7 markers are disclosed for detection to improve sensitivity and detection, but the number of target genes screened out in these detection methods is large, involving many primers, which are not suitable for actual clinical application

Images