The present invention discloses one kind of biochip for detecting the regulation and control of active medicine components on the expression of relevant genes. On one glass chip, one silicon chip or polymer chip, are fixed gene DNA probes for detecting PCNA, CyclinA1, CyclinB1, CyclinC, CyclinD1, CyclinD1, CyclinE1, CyclinG1, Cdk4, Cdk2, Cdc2, p16, p21, p53, E2F1, Chk1, Chk2, ATM, DNA-PK, DNA-Topo I, hTERT, Bcl-2, Trail, Mcl-1, Bax, Bak1, Brca1, Caspase3, Erk-3, Jnk, p38, Cdc25A, Fgd1, Rac and VEGF. The present invention can detect the regulation and control of active medicine components on the expression of relevant genes, and has the features of real-time, high sensitivity, high accuracy, high efficiency, etc.