Immune p16<INK4a> antigen preserving liquid used for thin prep liquid-based cytology test and preparation method of same

A technology of p16ink4a and thin-layer cells, which is applied in the field of clinical medical pathology, can solve the problems of false negative staining results, etc., and achieve the effect of protecting integrity, strong mucus ability, good stability and dispersion effect

Active Publication Date: 2017-10-20
GUANGZHOU JIANGYUAN MEDICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] p16 INK4a For the application in cytology, a suitable cell preservation solution is first required. The traditional liquid-based cell preservation solution has the functions of lysing red blood cells, diluting mucus, and fixing cells, which can meet the requi

Method used

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  • Immune p16&lt;INK4a&gt; antigen preserving liquid used for thin prep liquid-based cytology test and preparation method of same
  • Immune p16&lt;INK4a&gt; antigen preserving liquid used for thin prep liquid-based cytology test and preparation method of same
  • Immune p16&lt;INK4a&gt; antigen preserving liquid used for thin prep liquid-based cytology test and preparation method of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 An immune p16 for liquid-based thin layer cells INK4a Antigen Preservation Solution

[0044] The immune p16 for liquid-based thin layer cells INK4a Antigen preservation solution, composed of the following components and their concentrations: NaCl 85mmol / L, KCl 7.5mmol / L, edetate disodium 3.5mmol / L, urea 42mmol / L, sodium citrate 3.6mmol / L, sucrose 16mmol / L, PEG-400 6.5mmol / L, paraformaldehyde 2%, glycerol 8.4mmol / L and dithiothreitol 16.5mmol / L.

[0045] The immune p16 for liquid-based thin layer cells INK4a The preparation method of antigen preservation solution, comprises the following steps:

[0046] S1. Accurately weigh NaCl, KCl and urea, dissolve them in purified water in turn, stir to make them all dissolve, and obtain solution I;

[0047] S2. Accurately weigh disodium edetate and dissolve it in purified water, heat to dissolve it completely and then cool to room temperature to obtain solution II;

[0048] S3. Accurately weigh sodium citrate and su...

Embodiment 2

[0052] Example 2 An immune p16 for liquid-based thin layer cells INK4a Antigen Preservation Solution

[0053] The immune p16 for liquid-based thin layer cells INK4a Antigen preservation solution, composed of the following components and their concentrations: NaCl 60mmol / L, KCl 2mmol / L, disodium edetate 1mmol / L, urea 30mmol / L, sodium citrate 1mmol / L, sucrose 5mmol / L, PEG-400 4mmol / L, paraformaldehyde 2%, glycerin 2mmol / L and dithiothreitol 10mmol / L.

[0054] The immune p16 for liquid-based thin layer cells INK4a The preparation method of the antigen preservation solution is similar to Example 1.

Embodiment 3

[0055] Example 3 An immune p16 for liquid-based thin layer cells INK4a Antigen Preservation Solution

[0056] The immune p16 for liquid-based thin layer cells INK4a Antigen preservation solution consists of the following components and their concentrations: NaCl 100mmol / L, KCl 10mmol / L, EDTA disodium 5mmol / L, urea 50mmol / L, sodium citrate 5mmol / L, sucrose 20mmol / L, PEG-400 10mmol / L, paraformaldehyde 2.5%, glycerin 10mmol / L and dithiothreitol 20mmol / L.

[0057] The immune p16 for liquid-based thin layer cells INK4a The preparation method of the antigen preservation solution is similar to Example 1.

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Abstract

The invention belongs to the technical field of clinical medicine pathology and particularly relates to an immune p16<INK4a> antigen preserving liquid used for thin prep liquid-based cytology test and a preparation method of same. The antigen preserving liquid includes: 60-100 mmol/L of NaCl, 2-10 mmol/L of KCl, 1-5 mmol/L of EDTA disodium, 30-50 mmol/L of urea, 1-5 mmol/L of sodium citrate, 5-20 mmol/L of sucrose, 4-10 mmol/L of PEG-400, 2-3% of paraformaldehyde, 2-10 mmol/L of glycerol and 10-20 mmol/L of dithiothreitol. The preserving liquid is not only suitable for traditional slide preparation and staining of liquid-based cells, but also is suitable for cervical liquid-based cell p16<INK4a> protein immuno-labeling and staining, thus avoiding defects in conventional non-specific Papanicolaou staining.

Description

technical field [0001] The invention belongs to the technical field of clinical medical pathology, in particular to a p16 immune system for liquid-based thin layer cells INK4a Antigen preservation solution and preparation method thereof. Background technique [0002] Cervical cancer is one of the most common malignant tumors of the female reproductive system in gynecology. Its incidence is second only to breast cancer and ranks second among female malignant tumors. It seriously threatens women's health and quality of life. China is a country with a high incidence of cervical cancer, accounting for about 1 / 3 of the world's incidence and mortality. Cervical cancer is currently the only cancer whose etiology is clear and can be completely cured through effective early diagnosis and treatment. [0003] The current early diagnosis of cervical cancer and precancerous lesions is divided into "three steps". The first step is vaginal exfoliation cytology examination to screen out ...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 李小梅骆伟明李飞李高峰申令
Owner GUANGZHOU JIANGYUAN MEDICAL SCI & TECH CO LTD
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