60 results about "Liquid base cytology" patented technology

The invention discloses a simple thin liquid-based cytology examine method applied for the gynecological examination, the cervical cancer general examine and the cervical cancer preceding lesion of selected crowd, the steps including: using the cervical cells brush and the scraper to collect samples, put samples in the liquid-based cell preservation solution, add base liquid cell processing solution, oscillate, centrifugally separate, take and discard the supernatant samples liquid, using adding samples gun to assimilate sediment samples, smear, sheet drying, using 95% ethanol marinate or spray for fixed, washing and dyeing. The invention has simple method, small steps, high positive detection rate, low misdiagnosis rate, low cost, and it can effectively eliminate the red blood cells, mucus and other interference components from the cervical cytology detection specimens, with good sheet-producing result.

The invention relates to a tissue dewaxing transparent agent free of benzene, which is used in biological histology, histopathology or forensic science, can make a tissue transparent, and can make the tissue dewaxed. The tissue dewaxing transparent agent belongs to the technical field of in vitro diagnostic reagents. The tissue dewaxing transparent agent mainly contains 95%-100% of alicyclic hydrocarbon, and the balance of accessories; the alicyclic hydrocarbon is a monocyclic alicyclic hydrocarbon or a bicyclic alicyclic hydrocarbon. The tissue dewaxing transparent agent free of benzene has the advantages of being non-toxic, soft in effect, and not easy to cause shrinkage, deformation, hardening and embrittlement of a tissue material, capable of improving the section intact rate, insensitive to the humid environment, not easy to muddy due to absorption of moisture in the air, and the like; not only can be used for liquid based cytology, immunohistochemistry, biological histology, routine pathological diagnosis, and immunohistochemical diagnostic techniques, is also suitable for special staining method and histochemical techniques. The refractive index of used alicyclic hydrocarbon transparent agent (cyclohexane) is 1.42, the (methyl cyclohexane) refractive rate is 1.42, and is basically consistent with the tissue refractive index (1.418), and the transparent effect is better.

The invention relates to a liquid-based cell smear manufacturing and dyeing machine utilizing principles of injection and pipetting, which is characterized by structurally comprising a machine base, a machine body, a worktable, a horizontal slide rail, a moving arm, a horizontal motor moving device, an up-down motor moving device and a microcomputer motion control system, wherein four groups of smear manufacturing and dyeing units and a kit are mounted on the worktable board; and 24 samples can be automatically manufactured into smears and dyed when the machine runs once. The liquid-based cell smear manufacturing and dyeing machine does not need a tedious specific gravity liquid gradient centrifugation step, a micropipe type liquid drawing system of a liquid-based cytology test (LCT) machine, or processes of vacuum negative pressure drawing and positive pressure cell transfer of a microporous filter membrane of a Thinprep cytology test (TCT) machine. Cell samples are drawn, evenly mixed and scattered by using a pipetting injector and sample reagents are transferred by utilizing an accurate quantitative liquid drawing and injecting function of the pipetting injector. Cells in a settling and smear-manufacturing bin are naturally settled on a sticky glass slide by relying on the gravity of the cells and then automatically dyed. The liquid-based cell smear manufacturing and dyeing machine has the advantages that the operation is simple, the cost is relatively low, and high quality liquid-based cytological smears can be rapidly manufactured.

The invention relates to the field of liquid-based cytology immunocytochemistry, in particular to an immunohistochemical kit for auxiliary diagnosis of cervical cancer. Ready-to-use antibody working liquid is provided and prepared from an antibody reagent, bovine serum albumin, non-reductive sugar, betaine, cyclodextrin, a neutral inorganic salt, a surfactant and a preservative, wherein the antibody reagent is a reagent A or a reagent B; the reagent A is a phosphate buffer solution (PBS) of a p16<INK4a> monoclonal antibody and a Ki-67 monoclonal antibody; the reagent B is a PBS of horse radishperoxidase conjugated GaMIgG and an alkaline phosphatase conjugated goat anti-rabbit secondary antibody; and the pH value of the PBSs is 7.2-7.8.

An instrument for automated processing of sample vials are provided. The instrument causes an aliquot chamber within the vial to be filled with the fluid contained within the vial.

The invention relates to a software for daily concerns management in pathology department of a hospital and for assisting a doctor to made a fast and regularized diagnosis. The software is characterized in that tasks in the pathology department are systematically managed and various report databases on conventional pathology, cytology, liquid-based cytology and intraoperative freezing, and the like are integrated. An input mode for filling a small number of data and taking selection primarily facilitates the input operation. The software underscores providing various formative available templates and table templates for endoscopic biopsy and standard tumor reports; past examination documents of a patient are also provided; a database for disease diagnosis key points and pictures provides a diagnosis reference and fast-paste diagnosis description; tumor ICD-0 codes and corresponding standard diagnosis names are systematically provided so as to facilitate data organization; real-time diagnosis prompt after the diagnosis is made prevents diagnosis faults to a certain extent; and the programs are designed to assisting the doctor to made a fast and regularized pathology report.

The invention discloses a device and a method for making a liquid-based cytological smear in one step. The device comprises a sample centrifuge, a hanging basket which is installed on the sample centrifuge and a smear making bottle which is reversely placed in the hanging basket. The smear making bottle comprises a bottle cap, a bottle body and a filter screen which is installed between the bottle cap and the bottle body. Slide loading holes for loading glass slides are symmetrically arranged on the left and right of the side wall of the bottle cap. The size of the holes is slightly larger than the size of the glass slides. The method comprises that the steps that after the smear making bottle which contains sample cells is shaken, the glass slides are loaded in the smear making bottle and the smear is made in one step by conducting centrifugal treatment to the smear making bottle. The device and the method for making the liquid-based cytological smear in one step have the advantages that the effect of combining a liquid storage bottle and a centrifugal smear making chamber into one is realized, the centrifugal smear making process is simplified, the transfer link in the smear making process is omitted, the cell analysis, the cell settlement and the smear output process are completed in one step, and the stability of smear making quality is improved. As a device and a method for making cell smears, the device and the method for making the liquid-based cytological smear in one step can be widely used in the field of cell smear making.

The present invention provides a device and method for determining the adequacy of squamous (ectocervical) cells, columnar (endocervical) cells, neutrophils, and noncellular material in a liquid based cytology specimen. The invention first analyzes a liquid based cytology specimen using light scatter to create a light scatter characteristic representing a predetermined cell. Next the invention determines the presence of squamous (ectocervical) cells versus columnar (endocervical) cells versus neutrophils versus noncellular material using the results of the light scatter. The light scatter characteristic that may be used may be forward light scatter, side light scatter, or both side and forward light scatter.

The invention belongs to the technical field of clinical medical pathology and in particular relates to a p16INK4a immunolabeling color-developing kit for cervical liquid-based cells. The kit comprises a p16INK4a antigen preservation solution, a first antibody, a second antibody, a DAB (Diaminobenzidine) color-developing solution, an endogenous peroxidase blocking agent, confining liquid, a buffering solution, a positive reference substance and a negative reference substance. The kit provided by the invention has strong specificity; an antigen and antibody specific combination type dyeing method is utilized and the defect that the specificity of non-specific Papanicolaou staining and naked-eye appearance judgment of current liquid-based cytology are overcome.

The invention discloses a simple and quick preparation method of a thin-layer liquid-based cytology cell smear. The method is characterized by comprising the following steps: a, preprocessing a specimen; b, adding 5ml of adhesive separation liquid into a common centrifuge tube; c, centrifuging a sample mixture and discharging the precipitation from the centrifuge tube while reserving the supernate for use; d, placing a glass sheet with a written number into a long slot of a natural settling device fixing seat serving as corollary equipment for film making; e, placing a settling tube on the glass sheet; f, uniformly mixing the bottom sample left after the centrifugation of the step c, and adding the mixture into the settling tube; g, standing still and naturally settling for 15-30 minutes; h, rinsing the specimen with a washing liquid; i, fixing in 95% alcohol for 10 minutes, and performing Papanicolaou staining; and H, performing E staining or immunohistochemical staining and reading the film. Through the simple and quick preparation method of the thin-layer liquid-based cytology cell smear disclosed by the invention, the mucus, blood and inflammatory cells in the specimen can be separated by natural precipitation, the film is clear and the diagnosis accuracy is improved.

The invention relates to application of an enzyme-labeling-liquid-based cytology technology to the screening of bladder cancer. In the technology, a cell sample which is tabletted by a liquid-based tabletting method is treated by an acid phosphatase chemical staining method, and a bright red precipitate is formed in cytoplasm of abnormal cells by specific staining to ensure that the abnormal cells have remarkable marks. The invention provides a kit for the detection. The kit mainly comprises an enzyme-labeling-liquid-based cytology staining reagent, enzyme-labeling-liquid-based cytology preserving fluid and settlement buffer solution, and can provide high detection specificity and a good staining effect.

Disclosed are a tumor screening system, which operates separation of cells from a vial containing a solution mixed with the cells and collection of the cells on a slide using an automatic unit, a collection vial for liquid based cytology serving to simultaneously store a solution containing cells and pour the solution from out of tumor screening system, a brush for liquid based cytology of cervix carcinoma, which collects cells from a woman's uterus, and a supporting solution for cytodiagnosis, which preserves the collected cells.

The invention discloses a decision classification method for fusion reasoning and learning of liquid-based cytological examination, which comprises the following steps: 1) data and ontology preparation: constructing a data set for a decision target and an ontology related to the data, extracting a plurality of associated data features between the data set and the ontology, and taking the associated data features as sub-features of target features; 2) neural network and rule construction: training a neural network set comprising a target feature clustering device and a plurality of sub-featureclassifiers, and constructing an inference rule related to a decision target; and 3) knowledge reasoning and neural network fusion: fusing knowledge reasoning and machine learning to realize knowledgerule reasoning supporting a machine learning result, combining a reasoning result with an evolution method of the machine learning result, and analyzing a processing result. According to the method,two ways of knowledge reasoning and machine learning are fused, the classification accuracy is improved, meanwhile, the result has interpretability through an evolution method, and the reliability ofthe result is gradually improved.

The invention relates to a method for preparing a pulled type cervical smear. The method comprises the following steps: (1) oscillating a specimen through an oscillator; (2) centrifugating a part of the oscillated specimen until the specimen is layered into supernatant liquid and a cell layer; (3) removing the supernatant liquid, sucking the cell layer, dripping the cell layer on a glass slide, laminating the glass slide with another glass slide, and then pulling the glass slides to the opposite directions; (4) fixing and dyeing so as to prepare the smear. Compared with a conventional Pap smear method, the method provided by the invention has the advantages of high specimen satisfaction, high smear quality and the like. Compared with a liquid base cytology method, the method provided by the invention has the advantages of simplicity in operation, uniform tiling of the prepared smears, non-overlapping of cells, clear background, no precious equipment, low cost and the like, and is convenient to popularize. Compared with a human papillomavirus-deoxyribose nucleic acid (HPV-DNA) detection method, the method provided by the invention has the advantages of no use of precious equipment, low inspection cost, simplicity in operation, convenience in popularization and the like.

The invention belongs to the technical field of clinical medicine pathology and particularly relates to an immune p16<INK4a> antigen preserving liquid used for thin prep liquid-based cytology test and a preparation method of same. The antigen preserving liquid includes: 60-100 mmol / L of NaCl, 2-10 mmol / L of KCl, 1-5 mmol / L of EDTA disodium, 30-50 mmol / L of urea, 1-5 mmol / L of sodium citrate, 5-20 mmol / L of sucrose, 4-10 mmol / L of PEG-400, 2-3% of paraformaldehyde, 2-10 mmol / L of glycerol and 10-20 mmol / L of dithiothreitol. The preserving liquid is not only suitable for traditional slide preparation and staining of liquid-based cells, but also is suitable for cervical liquid-based cell p16<INK4a> protein immuno-labeling and staining, thus avoiding defects in conventional non-specific Papanicolaou staining.

The invention relates to a cell processing liquid for diagnosis of liquid based cytology, which comprises a sulfonic acid material, a non-ion type osmotic pressure regulator, polyoxyethylene octyl phenyl ether, formaldehyde and ethanol, wherein the sulfonic acid material is at least one of sulfuric acid, methanesulfonic acid or para-toluenesulfonic acid, and the non-ion type osmotic pressure regulator is dimethyl urea. The invention solves the problems that the current cell processing liquid can damage the cells and has a poor effect of removing impurities such as fragments of tissue, mucus, red blood cells and the like which have no values for diagnose, and the invention has low cost.

The invention discloses a manual semi-quantitative liquid-base cytological flaking method. Cervical exfoliated cells stored with liquid phase are used as a specimen source, centrifugation and semi-quantitative dilution are carried out, a cell dispersing agent is added to carry out suspending and evenly mixing, cell suspension is taken and dropped on the surface of a glass slide, and fixing and dyeing are carried out after the cells are settled and dried naturally. The method comprises the following steps: confirming and numbering a specimen; adding the cell dispersing agent; transferring the specimen and carrying out centrifugation; pouring supernate; diluting cell precipitation; transferring the cell suspension; and slide-dropping, and finishing flaking. The cervical liquid-base cytological flaking method of the invention is low-cost, highly-efficient, repeatable, and controllable, which solves the current problems including that imported automatic flaking equipment is expensive and matching reagents and consumables are too high in cost, domestic flaking systems are expensive but flaking quality is not stable, and slides prepared by traditional manual slide-dropping methods are rough. Thus, the flaking method of the invention is suitable for popularization in multi-level hospitals and medical organizations, especially in primary-level organizations.

The invention discloses a semi-automatic method for producing pleural and ascites liquid-based cytology. After the sample is collected, it is placed in a preservation solution bottle, and the preservation solution bottle is placed on a vibrator, and then passed through a centrifuge tube with a sharp bottom in a centrifuge. Add the cell dispersion liquid into the sampler, absorb and discard the supernatant in the test tube, remove the remaining liquid, and place the centrifuged sample on the sample delivery device of the film maker, and each sample passes through the spectrometer in turn, and the sample is aspirated The sample is placed on the corresponding slide in the transfer module, and the dried slide can be used for Papanicolaou staining or HE staining for cytological diagnosis, and Feulgen special staining for DNA quantitative analysis. The present invention is mainly used to improve and solve the problems existing in the non-cervical cell extraction, including the small number of cells, the overlapping of cells, the long production time, the background impurities of the produced films, and the low degree of automation. The quality of slides is improved, the number and distribution of cells on slides are more uniform, easy to use, and has the value of popularization and application.

The invention discloses a reagent kit for screening early-stage cervical cancers based on cervical liquid-based cytology and DNA methylation. The reagent kit comprises orange-G6, brilliant green liquid, Bismarck brown liquid, eosin Y liquid, a QlAamp DNA Mini Kit reagent kit body, a QIAamp EpiTect Bisulfite Kits reagent kit body and PCR reaction liquid, the forward primer sequence of JAM3-M4 is shown as SEQ ID No.6, the reverse primer sequence of JAM3-M4 is shown as SEQ ID No.7, the forward primer sequence of beta-actin is shown as SEQ ID No.16, the reverse primer sequence of beta-actin is shown as SEQ ID No.17. According to the reagent kit for screening the early-stage cervical cancers, detection flexibility, specificity, the positive predictive value and the negative predictive value meet the requirements of clinical diagnosis through primary verification of clinical tissue samples and further verification of a large amount of cervical exfoliated cytology.

The invention discloses a liquid-based thin-layer cell sheet making device. The liquid-based thin-layer cell sheet making device comprises a sample cup; a detachable filtering head and a detachable sheet making head are arranged on the sample cup sequentially; the sheet making head comprises a sheet making cylinder and a sheet making platform arranged in the sheet making cylinder; a cell precipitating sheet, which is protruded out of the end face of the sheet making platform and is opposite to the filtering head, is arranged on the sheet making platform; an outlet pipe communicated with the filtering head is arranged at the outer end of the sheet making platform; a slide clamping groove positioned between the filtering head and the sheet making platform is arranged on the sheet making cylinder; the cell precipitating sheet is protruded out of the end face, adjacent to the cell precipitating sheet, of the slide clamping groove and can abut against a slide inserted into the slide clamping groove. The liquid-based thin-layer cell sheet making device integrates sampling, filtering and separation, precipitation cell enrichment and cell tissue transfer, the sample cup does not need to bechanged in the process, the sample does not need to be transferred, and liquid-based cell sheet making can be completed only by manually inserting the slide, and the liquid-based thin-layer cell sheet making device is simple in structure, convenient to use and suitable for popularization.

The invention provides a liquid separation device for screening cervical cancer by combining liquid-based cytology and human papilloma virus. The liquid separation device comprises a sealing cover, and the sealing cover is used for being hermetically connected with a bottle opening of an elution bottle; an air inlet pipe is arranged on the sealing cover, one end of the air inlet pipe communicates with an inner cavity of the elution bottle, and the other end of the air inlet pipe is connected with an air bag; and a liquid inlet pipe is further arranged on the sealing cover, one end of the liquid inlet pipe extends into the position, close to the bottom, in the elution bottle, the other end of the liquid inlet pipe is connected with a liquid inlet of a liquid separation valve, the liquid separation valve is further provided with a first liquid separation opening and a second liquid separation opening, and the first liquid separation opening and the second liquid separation opening communicate with the liquid inlet. According to the liquid separation device for screening cervical cancer by combining liquid-based cytology and human papilloma virus, a sample can be correspondingly and independently mixed with a preservation solution and a lysis solution by one-time collection, so that the uniformity and independence of the sample are ensured, and the situation that the final detection result is influenced due to the fact that the samples collected twice are different is avoided, so that the accuracy of TCT and cervical HPV-DNA combined detection is improved.

The invention provides an H-E staining solution for liquid-based cytology experiments, and relates to the technical field of biological preparations. The H-E staining solution includes a modified hematoxylin staining solution and a modified eosin staining solution. The modified hematoxylin staining solution includes sodium iodate, hematoxylin, aluminium potassium sulfate, absolute alcohol, formaldehyde, glacial acetic acid and water. The modified eosin staining solution includes alcohol-soluble eosin, absolute alcohol, aluminium potassium sulfate, sodium iodate, water-soluble eosin, phloxine,glacial acetic acid, formaldehyde and deionized water. By utilizing the H-E staining solution, the cost of use is reduced, time for sample preparation is saved, and the H-E staining solution in matchwith the liquid-based cytology experiments enables uniform staining, clear tissue structures after staining, and bright cell color, and facilitates observation and judgment in liquid-based cytology section production.

A method of determining the presence or absence of a target nucleic acid in a cell sample, said method comprising: a) contacting a surface comprising anion exchange moieties with the sample under conditions suitable to induce binding between the cells and said surface; b) separating the surface with the bound cells from the remaining sample to collect the cells; c) releasing nucleic acids from the cells and d) generating a hybrid between the target nucleic acid and a probe specific for the target nucleic acid e) detecting the presence or absence of the hybrid. The present invention is provides a rapid and automatable method, wherein cells, such as epithelial cells originating from cervical swab samples, are collected from the surrounding liquid medium, such as a liquid based cytology medium, by binding them to an anion exchange surface. The cells bind directly with high affinity and quick kinetics to the anion exchange surface which preferably is provided by magnetic particles carrying anion exchange moieties. The cells that are bound to the anion exchange surface can be easily separated from the surrounding medium and can be directly resuspended in a composition that is suitable for a subsequent hybrid capturing assay which is performed in step d) to detect e. g. pathogen nucleic acids such as HPV nucleic acids.

The invention provides cell extraction solution and an application thereof, and belongs to the technical field of agents. The cell extraction solution comprises, by weight, 29-35 parts of glycerol, 0.005-0.015 part of potassium chloride, 0.01-0.03 part of monopotassium phosphate, 0.1-0.2 part of sodium hydrogen phosphate, 0.5-1 part of sodium chloride, 0.4-0.8 part of ethylene diamine tetraaceticacid, 0.05-0.15 part of phenoxyethanol and 63-69 parts of water. By the aid of the cell extraction solution, mucus and leucorrhea in a sample can be removed, squamous cells can be extracted, so that impurities and the squamous cells are separated, a doctor conveniently reads pieces and diagnoses, and the efficiency and the accuracy rate of a doctor in liquid-based cytological diagnosis.

The invention provides a cell collecting device of a membrane-type liquid-based cytology slide processor. A liquid level sensor connected electrically with a control system is arranged in a filter membrane barrel to transmit a liquid level signal in the filter membrane barrel to the control system to accurately control the action time of a negative pressure air pump so as to collect cells more effectively and safely. The invention also provides a method for collecting the cells by using the cell collecting device in the membrane-type liquid-based cell film maker. The device and the method canadjust the cell collecting time of the membrane-type liquid-based cytology slide processor instantly according to the actual situation of a sample so as to prevent situations of filter membrane explosion, air pump idling or insufficient cell collection.

The invention provides a sample extracting liquid for liquid-based cytology experiments, which comprises sodium azide, potassium chloride, ethanol, ethylene glycol, benzalkonium chloride, sodium dodecyl sulfate, glacial acetic acid, and purified water. According to the extracting liquid, the cost of use is reduced, and impurities such as erythrocyte and mucus masses in a sample can be effectivelydecomposed and removed to obtain effective cells to be tested, and therefore, slide reading backgrounds in liquid-based cytology experiments are clear, and cast-off cells having diagnostic significance are complete in shape, easy to distinguish and uniform, and are paved on a section making region of a glass slide almost without stacking.