Reagent kit for screening early-stage cervical cancers based on cervical liquid-based cytology and DNA methylation

Abstract

The invention discloses a reagent kit for screening early-stage cervical cancers based on cervical liquid-based cytology and DNA methylation. The reagent kit comprises orange-G6, brilliant green liquid, Bismarck brown liquid, eosin Y liquid, a QlAamp DNA Mini Kit reagent kit body, a QIAamp EpiTect Bisulfite Kits reagent kit body and PCR reaction liquid, the forward primer sequence of JAM3-M4 is shown as SEQ ID No.6, the reverse primer sequence of JAM3-M4 is shown as SEQ ID No.7, the forward primer sequence of beta-actin is shown as SEQ ID No.16, the reverse primer sequence of beta-actin is shown as SEQ ID No.17. According to the reagent kit for screening the early-stage cervical cancers, detection flexibility, specificity, the positive predictive value and the negative predictive value meet the requirements of clinical diagnosis through primary verification of clinical tissue samples and further verification of a large amount of cervical exfoliated cytology.

Description

technical field [0001] The invention relates to a kit for screening early cervical cancer based on cervical liquid-based cytology and DNA methylation. Background technique [0002] Cervical cancer is one of the common gynecological malignancies. There are about 528,000 new cases and 266,000 deaths in the world every year, and about 85% of them occur in developing countries. The incidence rate in China remains high at 7.5 / 100,000 and the death rate is 3.4 / 100,000. In some backward areas that lack reasonable screening, the incidence rate is even as high as 81 / 100,000. According to preliminary estimates, by 2030, there will be approximately 474,000 cervical cancer deaths per year, of which more than 95% may occur in backward and developing countries. Therefore, it is of great significance to find an effective method for early screening of cervical cancer, to screen out cervical precancerous lesions and cancer patients, and to implement effective early diagnosis and early trea...

AI Technical Summary

Problems solved by technology

[0005] Researchers at home and abroad have made exciting progress in the early screening of cervical cancer by using tumor suppressor gene methylation detection. However, there are large differences between different studies of the same gene. The reasons may be as follows: 1. Specificity of gene methylation sites; 2. Differences in genetic background of genes; 3. Heterogeneity of operation methods and experimental reagents

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