A purpose of the invention is to disclose a liquid-based cytology detection method, which comprises: 1, numbering selected bloody and mucus specimens according to centrifugal tubes, wherein a group comprises eight specimens; 2, placing the specimens in the steps 1 into a centrifuge, and carrying out centrifugation for 5 min at 2500 r/min; 3, pouring the upper layer storage clarification liquid obtained after the centrifugation into a corresponding specimen storage bottle, adding 30 ml of 10% glacial acetic acid to downwardly precipitated cell mass, mucus mass and blood cell mass, covering with a lid, placing into a shaker, and carrying out oscillation for 8-10 min; 4, carrying out centrifugation on the oscillated product obtained in the step 3 for 5 min at 2500 r/min, removing the upper layer mixing liquid after the centrifugation, mixing the lower layer cell mass and a corresponding storage liquid, and pouring the obtained mixture into a storage bottle; 5, producing a liquid-based thin-layer cell sheet gynecological specimen from the product obtained in the step 4; and 6, staining the liquid-based thin-layer cell sheet obtained in the step 5, carrying out sealed fixation, and carrying out air drying. The liquid-based cytology detection method of the present invention has advantages of simple, convenient and flexible operation and substantially improved detection accuracy.