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P16INK4a immunolabeling color-developing kit for cervical liquid-based cells

A p16ink4a, liquid-based cell technology, applied in the field of clinical medical pathology, can solve problems such as limitations, non-specificity of immunostaining, false negative staining results, etc., and achieve the effects of avoiding the influence of antigens, strong specificity, and shortening the reaction time

Active Publication Date: 2017-09-26
GUANGZHOU JIANGYUAN MEDICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] However, the particularity of cervical liquid-based cell samples limits the development of its application: (1) cervical liquid-based samples often contain more inflammatory cells, red blood cells and other interfering components, and are rich in endogenous peroxidase, which can easily lead to immune Non-specific staining; (2) The current liquid-based cell preservation solution cannot preserve the p16 of cells well INK4a Antigens, which can easily lead to false negative staining results

Method used

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  • P16INK4a immunolabeling color-developing kit for cervical liquid-based cells
  • P16INK4a immunolabeling color-developing kit for cervical liquid-based cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 A method for cervical liquid-based cell p16 INK4a Immunolabeling Chromogenic Kit

[0049] The kit includes: p16 INK4a Antigen Preservation Solution, Primary Antibody, Secondary Antibody, DAB Chromogenic Solution, Endogenous Peroxidase Blocker, Blocking Solution, Buffer, Positive Control and Negative Control.

[0050] Among them, the p16 INK4a The antigen preservation solution is composed of the following components and their concentrations: NaCl 85mmol / L, KCl7.5mmol / L, edetate disodium 3.5mmol / L, urea 42mmol / L, sodium citrate 3.6mmol / L, sucrose 16mmol / L L, PEG-400 6.5mmol / L, paraformaldehyde 2%, glycerin 8.4mmol / L and dithiothreitol 16.5mmol / L.

[0051] The primary antibody is p16 INK4a mouse anti-human monoclonal antibody; the second antibody is a rabbit anti-mouse IgG antibody labeled with horseradish peroxidase.

[0052] The endogenous peroxidase blocking agent is 0.75% periodic acid solution; the blocking solution is animal non-immune goat serum.

[...

Embodiment 2

[0055] Example 2 A method for cervical liquid-based cell p16 INK4a Immunolabeling Chromogenic Kit

[0056] A liquid-based cell p16 for cervix INK4a Immunolabeling chromogenic kit, the kit includes: p16 INK4a Antigen Preservation Solution, Primary Antibody, Secondary Antibody, DAB Chromogenic Solution, Endogenous Peroxidase Blocker, Blocking Solution, Buffer, Positive Control and Negative Control.

[0057] The p16 INK4a The antigen preservation solution is composed of the following components and their concentrations: NaCl 60mmol / L, KCl 2mmol / L, disodium edetate 1mmol / L, urea 30mmol / L, sodium citrate 1mmol / L, sucrose 5mmol / L, PEG -4004mmol / L, paraformaldehyde 2%, glycerol 2mmol / L and dithiothreitol 10mmol / L.

[0058] Wherein, the first antibody is p16 INK4a mouse anti-human monoclonal antibody; the second antibody is a rabbit anti-mouse IgG antibody labeled with horseradish peroxidase.

[0059] The endogenous peroxidase blocking agent is 0.5% periodic acid solution; the b...

Embodiment 3

[0062] Example 3 A method for cervical liquid-based cell p16 INK4a Immunolabeling Chromogenic Kit

[0063] A liquid-based cell p16 for cervix INK4a Immunolabeling chromogenic kit, the kit includes: p16 INK4a Antigen Preservation Solution, Primary Antibody, Secondary Antibody, DAB Chromogenic Solution, Endogenous Peroxidase Blocker, Blocking Solution, Buffer, Positive Control and Negative Control.

[0064] Among them, the p16 INK4a The antigen preservation solution is composed of the following components and their concentrations: NaCl 100mmol / L, KCl10mmol / L, EDTA disodium 5mmol / L, urea 50mmol / L, sodium citrate 5mmol / L, sucrose 20mmol / L, PEG- 40010mmol / L, paraformaldehyde 2.5%, glycerol 10mmol / L and dithiothreitol 20mmol / L.

[0065] The first antibody is p16 INK4a mouse anti-human monoclonal antibody; the second antibody is a rabbit anti-mouse IgG antibody labeled with horseradish peroxidase.

[0066] The endogenous peroxidase blocking agent is 1% periodic acid solution; t...

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Abstract

The invention belongs to the technical field of clinical medical pathology and in particular relates to a p16INK4a immunolabeling color-developing kit for cervical liquid-based cells. The kit comprises a p16INK4a antigen preservation solution, a first antibody, a second antibody, a DAB (Diaminobenzidine) color-developing solution, an endogenous peroxidase blocking agent, confining liquid, a buffering solution, a positive reference substance and a negative reference substance. The kit provided by the invention has strong specificity; an antigen and antibody specific combination type dyeing method is utilized and the defect that the specificity of non-specific Papanicolaou staining and naked-eye appearance judgment of current liquid-based cytology are overcome.

Description

technical field [0001] The invention belongs to the technical field of clinical medical pathology, and in particular relates to a p16 for cervical liquid-based cells INK4a Immunolabeling Chromogenic Kit. Background technique [0002] Cervical cancer is one of the most common malignant tumors of the female reproductive system in gynecology. Its incidence is second only to breast cancer and ranks second among female malignant tumors. It seriously threatens women's health and quality of life. China is a country with a high incidence of cervical cancer, accounting for about 1 / 3 of the world's incidence and mortality. Cervical cancer is currently the only cancer whose etiology is clear and can be completely cured through effective early diagnosis and treatment. [0003] The current early diagnosis of cervical cancer and precancerous lesions is divided into "three steps". The first step is vaginal exfoliation cytology examination to screen out suspicious cases. The second step...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/535
CPCG01N33/535G01N33/57442
Inventor 李小梅骆伟明李飞李高峰申令
Owner GUANGZHOU JIANGYUAN MEDICAL SCI & TECH CO LTD
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