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Immunolabeling is a biochemical process that enables the detection and localization of an antigen to a particular site within a cell, tissue, or organ. Antigens are organic molecules, usually proteins, capable of binding to an antibody. These antigens can be visualized using a combination of antigen-specific antibody as well as a means of detection, called a tag, that is covalently linked to the antibody. If the immunolabeling process is meant to reveal information about a cell or its substructures, the process is called immunocytochemistry. Immunolabeling of larger structures is called immunohistochemistry.

Infectious cDNA clones of porcine reproductive and respiratory syndrome virus and expression vectors thereof

InactiveUS6841364B2SsRNA viruses positive-senseSugar derivativesRespiratory syndrome virusGene mutation

Infectious cDNA clones of North American Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) are provided. Further provided are cDNA clones comprising genetic mutations. Also provided are vaccines comprising cDNAs, including genetically and immunologically marked vaccines for North American PRRSV.

Infectious cDNA clones of porcine reproductive and respiratory syndrome virus and expression vectors thereof

Infectious cDNA clones of porcine reproductive and respiratory syndrome virus and expression vectors thereof

Infectious cDNA clones of porcine reproductive and respiratory syndrome virus and expression vectors thereof

Owner:PROTATEK INT

Antibody complexes and methods for immunolabeling

InactiveUS20070269902A1Eliminate needTime requiredMaterial nanotechnologyMicrobiological testing/measurementFluorescenceAntibody fragments

The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling reagents have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab′ fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.

Antibody complexes and methods for immunolabeling

Antibody complexes and methods for immunolabeling

Antibody complexes and methods for immunolabeling

Owner:MOLECULAR PROBES

Spectinomycin fast time-resolved fluoroimmunoassay quantitative detection test strip

InactiveCN103792357AHigh sensitivityDetection has no effectMaterial analysisAntigenCellulose

The invention relates to a preparation method and an application of a spectinomycin fast time-resolved fluoroimmunoassay quantitative detection test strip. The test strip consists of a Fusion5 membrane, a nitrocellulose membrane and water absorption paper; quick and fast quantitative detection is performed on the antigen to be detected by taking a time-resolved fluorescent microsphere as an immune marker based on the principle of competition law.

Spectinomycin fast time-resolved fluoroimmunoassay quantitative detection test strip

Spectinomycin fast time-resolved fluoroimmunoassay quantitative detection test strip

Owner:JIANGSU WISE SCI & TECH DEV

Antibody complexes and methods for immunolabeling

InactiveUS20090124511A1The process is convenient and fastEasy to adjustPeptide librariesHydrolasesFluorescenceAntibody fragments

The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab′ fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.

Antibody complexes and methods for immunolabeling

Antibody complexes and methods for immunolabeling

Antibody complexes and methods for immunolabeling

Owner:LIFE TECH CORP

Preparation for cadmium antimonide quantum dot immune marker and detection method for electrochemical sandwich immune

InactiveCN101526523AImprove stabilityHigh sensitivityMaterial electrochemical variablesMicrosphereSilicon dioxide

The invention relates to preparation for a cadmium antimonide quantum dot immune marker and a detection method for electrochemical sandwich immune, having the signal amplification function. Through the reaction of silicon dioxide particle and reacting ATPS, an active amido group is modified on the surface of the silicon dioxide particle under room temperature. The silicon dioxide particle is then reacted with carboxyl on the surface of a quantum dot to form a silicon dioxide microsphere with the surface modified with the quantum dot. The silicon dioxide microsphere after being modified is further modified with a second antibody on the surface of the quantum dot under the actions of EDC and NHS, and a cadmium antimonide quantum dot immune marker is obtained. The detection method for the electrochemical sandwich immune utilizes anti-AFP to modify Fe3O4 magnetic nano-microsphere, thereby Si/QD/Ab2 is modified on magnetic particles, and then electrochemical determination is carried out. Because the surface of the marker is rich in the quantum dots, the amount of the quantum dot loaded in a single sandwich immune process is greatly increased, dissolving-out voltammetry detection signals of an anode are amplified, and the sensitivity of low-concentration biomolecule detection is greatly enhanced.

Preparation for cadmium antimonide quantum dot immune marker and detection method for electrochemical sandwich immune

Preparation for cadmium antimonide quantum dot immune marker and detection method for electrochemical sandwich immune

Preparation for cadmium antimonide quantum dot immune marker and detection method for electrochemical sandwich immune

Owner:SOUTHEAST UNIV

Quantitative in situ characterization of biological samples

ActiveUS20150133321A1Image enhancementImage analysisImmune markersStromal region

The present disclosure relates to characterization of biological samples. By way of example, a biological sample may be contacted with a plurality of probes specific for targets in the sample, such as probes for immune markers and segmenting probes. Acquired image data of the sample may be used to segment the images into epithelial and stromal regions to characterize individual cells in the sample based on the binding of the probes. Further, the biological sample may be characterized by a distribution, location, and type of a plurality of the characterized cells.

Quantitative in situ characterization of biological samples

Quantitative in situ characterization of biological samples

Quantitative in situ characterization of biological samples

Owner:LEICA MICROSYSTEMS CMS GMBH

Methods for phenotyping of intact whole tissues

ActiveUS20150087001A1Reduced feature requirementsPreparing sample for investigationBiological testingWhole bodyTissue clearing

In various embodiments, the present application teaches methods and compositions for tissue clearing in which whole organs and bodies are rendered macromolecule-permeable and optically-transparent, thereby exposing their cellular structure with intact connectivity. In some embodiments, the present application teaches PACT, a protocol for passive tissue clearing and immunostaining of intact organs. In other embodiments, the present application teaches RIMS, a refractive index matching media for imaging thick tissue. In yet other embodiments, the application teaches PARS, a method for whole-body clearing and immunolabeling.

Methods for phenotyping of intact whole tissues

Methods for phenotyping of intact whole tissues

Methods for phenotyping of intact whole tissues

Owner:CALIFORNIA INST OF TECH

Combined assay kit for premature birth of pregnant woman and fetal membrane premature rupture

InactiveCN102445534AAvoid contractionsAvoid increased risk of infectionMaterial analysisFoetal membranesBiology

The invention relates to a combined assay kit for premature birth of a pregnant woman and fetal membrane premature rupture, which comprises a premature birth and fetal membrane premature rupture immunochromatographic assay test strip or comprises a premature birth immunochromatographic assay test strip and a fetal membrane premature rupture immunochromatographic assay test strip. More preferably, the combination mat and the nitrocellulose membrane of the premature birth immunochromatographic assay test strip are respectively provided with a premature birth assay immunolabelling antibody and a premature birth assay antibody; the combination mat and the nitrocellulose membrane of the fetal membrane premature rupture immunochromatographic assay test strip are respectively provided with a fetal membrane premature rupture assay immunolabelling antibody and a fetal membrane premature rupture assay antibody, or the immunolabelling antibodies and the antibodies are respectively coated onto the combination mats and the nitrocellulose membranes of the premature birth and fetal membrane premature rupture immunochromatographic assay test strip. The combined assay kit for the premature birth of the pregnant woman and the fetal membrane premature rupture is capable of simultaneously diagnosing the premature birth and the fetal membrane premature rupture through primary sampling, is simple and convenient in application, and further, is capable of improving the accuracy of premature birth diagnosis. A powerful basis is provided for the next accurate treatment by combined assay results. The combined assay kit for the premature birth of the pregnant woman and the fetal membrane premature rupture is suitable for popularization and application in a large scale.

Combined assay kit for premature birth of pregnant woman and fetal membrane premature rupture

Combined assay kit for premature birth of pregnant woman and fetal membrane premature rupture

Combined assay kit for premature birth of pregnant woman and fetal membrane premature rupture

Owner:WUXI CITY KAIAOSHAN BIOPHARML TECH +2

Construction and application of recombinant peste des petits ruminants virus expressing fused exogenous epitope N protein

InactiveCN103224914AMicroorganism based processesAntiviralsEpitopeMarker vaccine

The invention relates to the field of immune marking vaccines and in particular relates to construction and an application of recombinant peste des petits ruminants virus expressing fused exogenous epitope N protein.

Construction and application of recombinant peste des petits ruminants virus expressing fused exogenous epitope N protein

Construction and application of recombinant peste des petits ruminants virus expressing fused exogenous epitope N protein

Construction and application of recombinant peste des petits ruminants virus expressing fused exogenous epitope N protein

Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI

Magnetic sensing identification method for high-flux multi-channel low-abundance biomolecules

ActiveCN104034881AHigh sensitivityImprove throughputBiological testingMaterial magnetic variablesAntigenSomatic cell

The invention relates to a magnetic sensing identification method for high-flux multi-channel low-abundance biomolecules, and belongs to the technical field of biomolecule identification. The invention mainly aims at an immunolabelling biomolecule detection, monitoring and identification method based on systems such as antigens-antibodies, cell factors-cell factor acceptors, bioactive peptides-acceptors, biotin-avidin and chiral molecules. A biomolecule probe and detection system consists of superparamagnetic particles and high-performance magnetic sensors. The magnetic sensing identification method can be applied to the application fields of biology, medicines, food safety and the like, and can be used for biomolecule identification, detection and monitoring, clinical disease diagnose, food safety detection and virus and germ detection.

Magnetic sensing identification method for high-flux multi-channel low-abundance biomolecules

Magnetic sensing identification method for high-flux multi-channel low-abundance biomolecules

Magnetic sensing identification method for high-flux multi-channel low-abundance biomolecules

Owner:NANJING YIDEGUAN ELECTRONICS TECH

System for predicting prognosis of gastric cancer in subjects

ActiveCN111724903AImproved prognosisMedical data miningHealth-index calculationGastric carcinomaOncology

The invention provides a system for predicting prognosis of gastric cancer of subjects. The system comprises: a data acquisition module, which is used for acquiring clinical characteristic data of thesubjects, immune marker characteristic data of the subjects and protein expression data of the subjects; a data processing module, which is used for further processing the data acquired in the data acquisition module; and a module for calculating the prognosis risk of the gastric cancer of the subjects, wherein the module utilizes the data processed in the data processing module to calculate theprognosis risk values of the gastric cancer of the subjects, and groups the subjects based on the risk values. According to the system and a method provided by the invention, eight characteristics, including five immunomarkers (CD3, CD4, PDL1, PAX5, and GZMB), an EMT protein marker (CDH1) and two clinical characteristics (pTNM and age), are selected to develop the system and the method that can significantly improve prognostic ability of gastric cancer patients. The system and the method may be applicable to patients with or without neoadjuvant chemotherapy and exhibit predicted values, and the patients may benefit from post-operative adjuvant chemotherapy.

System for predicting prognosis of gastric cancer in subjects

System for predicting prognosis of gastric cancer in subjects

System for predicting prognosis of gastric cancer in subjects

Owner:BEIJING CANCER HOSPITAL PEKING UNIV CANCER HOSPITAL

P16INK4a immunolabeling color-developing kit for cervical liquid-based cells

ActiveCN107202888AImprove protectionPrevent closureMaterial analysisLiquid base cytologyAntigen

The invention belongs to the technical field of clinical medical pathology and in particular relates to a p16INK4a immunolabeling color-developing kit for cervical liquid-based cells. The kit comprises a p16INK4a antigen preservation solution, a first antibody, a second antibody, a DAB (Diaminobenzidine) color-developing solution, an endogenous peroxidase blocking agent, confining liquid, a buffering solution, a positive reference substance and a negative reference substance. The kit provided by the invention has strong specificity; an antigen and antibody specific combination type dyeing method is utilized and the defect that the specificity of non-specific Papanicolaou staining and naked-eye appearance judgment of current liquid-based cytology are overcome.

P16INK4a immunolabeling color-developing kit for cervical liquid-based cells

P16INK4a immunolabeling color-developing kit for cervical liquid-based cells

P16INK4a immunolabeling color-developing kit for cervical liquid-based cells

Owner:GUANGZHOU JIANGYUAN MEDICAL SCI & TECH CO LTD

Micro-fluidic chip based on magnetic bead coated antibody and method for capturing cardiac markers

ActiveCN105424922AImprove capture abilityEnsure identificationMaterial analysisMagnetic beadAntigen binding

The embodiment of the invention discloses a micro-fluidic chip based on a magnetic bead coated antibody and a method for capturing cardiac markers. The micro-fluidic chip based on the magnetic bead coated antibody and the method for capturing cardiac markers are applied to the field of immunomagnetic bead coated binding antibodies, and prevent coated antibodies from being redissolved in a fluid system or being captured by antigens in fluid and disengaged from a base material. The micro-fluidic chip comprises a sample injection chamber, a Y-shaped structure channel and recycling chambers. The Y-shaped structure channel comprises a funnel-shaped channel at the upper portion and a columnar channel at the lower portion. An immunolabelling module is arranged at the bottom of the funnel-shaped channel. A blood filtering module is arranged at the upper portion of the immunolabelling module. The bottom end of the columnar channel at the lower portion is forked to form a pair of parallel immunity channels. The tail end of each immunity channel is connected with one recycling chamber. The columnar channel is provided with an upper cavity and a lower cavity. The upper cavity is a strong magnetism cavity, and the lower cavity is a quality control cavity.

Micro-fluidic chip based on magnetic bead coated antibody and method for capturing cardiac markers

Micro-fluidic chip based on magnetic bead coated antibody and method for capturing cardiac markers

Micro-fluidic chip based on magnetic bead coated antibody and method for capturing cardiac markers

Owner:北京乐普诊断科技股份有限公司

NTx (Naltrexone) detection test paper, kit and preparation method thereof

InactiveCN107688090AImprove detection stabilityEnables direct detectionMaterial analysisOsteoporosisTest strips

The invention discloses an NTx (Naltrexone) detection test strip. The NTx detection test strip comprises a chromatography membrane and a conjugate pad; an immunolabelled NTx antibody and an immunolabelled first antibody are loaded on the conjugate pad; the chromatography membrane is provided with an NTx protein covered detection line and a second antibody covered colorimetric line along a liquid chromatography direction. When a sample is detected, the colorimetric line correspondingly displays the chroma of specific protein concentration after the second antibody is specifically combined withthe first antibody; NTx protein on the detection line and the NTx protein in the sample are competitively combined with the immunolabelled NTx antibody and the color developing depth of the detectionline and the colorimetric line is directly compared so that semi-quantitative detection of the NTx protein is realized. The invention discloses a preparation method of the NTx detection test strip; the preparation method is used for preparing an NTx protein semi-quantitative detection test strip which is rapid and sensitive in detection and does not depend on an instrument. The invention disclosesan NTx detection kit; the NTx detection kit comprises the NTx detection test strip and can provide effective information for early diagnosis and treatment of osteoporosis.

NTx (Naltrexone) detection test paper, kit and preparation method thereof

NTx (Naltrexone) detection test paper, kit and preparation method thereof

NTx (Naltrexone) detection test paper, kit and preparation method thereof

Owner:RUNBIO BIOTECH CO LTD

Non-covalent patterned chemical features and use thereof in MALDI-based quality control

ActiveUS9970932B2Rapid assessmentQuick screeningMicrobiological testing/measurementMaterial analysis by electric/magnetic meansBiochemistryPeptide array

The present application provides arrays for use in immunosignaturing and quality control of such arrays. Also disclosed are peptide arrays and uses thereof for diagnostics, therapeutics and research.

Non-covalent patterned chemical features and use thereof in MALDI-based quality control

Non-covalent patterned chemical features and use thereof in MALDI-based quality control

Non-covalent patterned chemical features and use thereof in MALDI-based quality control

Owner:ARIZONA STATE UNIVERSITY

Recombinant bacterium of brucella abortus with immunity labeling and use thereof

InactiveCN101386831ALow toxicityImprove purification effectAntibacterial agentsBacterial antigen ingredientsBrucella abortusAnimal use

The invention discloses a recombinant strain for Brucella abortus provided with an immune label. The recombinant strain for the Brucella melitensis provided with the immune label is a strain obtained by killing encoding genes of perosamine synthetase in the Brucella abortus. Compared with an original strain, the obtained strain has lower virulence, and can be used as a vaccine to immunize animals without killing the genes. By utilization of the strain to immunize a mouse, immunoreaction states different from those of the standard strain and the prior vaccine strain can be generated in the body of the mouse, and sicken animals can be identified from immune animal groups by immunological detection of serum of the animals. The modification of the brucelliasis vaccine and the research of a diagnosis and identification agent have important significance in developing a brucelliasis gene deletion marker vaccine and a matched diagnosis and identification agent and promoting the elimination and purification of brucelliasis of the animals all over the world.

Recombinant bacterium of brucella abortus with immunity labeling and use thereof

Recombinant bacterium of brucella abortus with immunity labeling and use thereof

Recombinant bacterium of brucella abortus with immunity labeling and use thereof

Owner:CHINA AGRI UNIV

Antibody complexes and methods for immunolabeling

InactiveUS8323903B2The process is convenient and fastEasy to adjustMaterial nanotechnologyNanoinformaticsAntibody fragmentsProtein G

The present invention provides novel immunolabeling complexes and certain components of such complexes, as well as methods of preparing and using such complexes, and kits for use in preparing labeling proteins and for immunolabeling. The pre-formed immunolabeling complexes of the invention comprise both a target-binding antibody and a labeling protein that contains covalently attached labels, where the labeling protein binds selectively and with high affinity to a selected region of the target-binding antibody. Novel labeling proteins of the invention include non-antibody peptides and proteins, such as a complex of protein G and a labeled albumin, and monovalent antibody fragments, such as labeled Fab fragments of an anti-Fc antibody. In methods of the invention, the preformed immunolabeling complexes are added to the sample alone or in combination, for purposes of labeling and optionally detecting the target of interest.

Antibody complexes and methods for immunolabeling

Antibody complexes and methods for immunolabeling

Antibody complexes and methods for immunolabeling

Owner:LIFE TECH CORP

Immortalized telocytes system and construction method thereof

ActiveCN107034238AResolve separabilityAddressing Purity IssuesGenetically modified cellsVirus peptidesAntigenCulture mediums

The invention relates to a construction method of an immortalized telocytes system. The construction method comprises the following steps: (1) digesting shorn off mouse lung tissue block in type-II collagenase, carrying out filtering and centrifuging, collecting cyte precipitate, culturing the cyte precipitate in a culture medium, after fibrocytes are attached to a wall, transferring supernate to another culture dish, culturing for 12 hours, then changing liquid, and continuing culturing for 3-5 days; (2) taking a trace spearhead as a cyte scraper to scrape off the fibrocytes, and after scraping off the fibrocytes repeatedly, observing a specificity structure telopode and identifying telocytes by an immune marker; and (3) taking the telocytes as host cells, infecting the host cells by using a retroviral vector which contains SV-40-large tumor antigen, and carrying out passage, screening and identifying. Even if the immortalized telocytes system is cultured to the 50th generation, the state is still stable, and therefore, the problem that the purity and the stability of the telocytes cannot be guaranteed by repeated separation and extraction in the prior art.

Immortalized telocytes system and construction method thereof

Immortalized telocytes system and construction method thereof

Immortalized telocytes system and construction method thereof

Owner:ZHONGSHAN HOSPITAL FUDAN UNIV

Immunofluorescence label reagent kit

InactiveCN101149372ASimple and fast operationImprove stabilityBiological testingFluorescenceImmunofluorescent labeling

This invention discloses to a kind of immunofluorescence mark reagent box. It is a reagent box applied for the aspects of immunity mark which uses the green fluorescence protein (GFP) as the indicator, the staphylococcus protein A (SPA) as the antibody. It also discloses the preparation method of the reagent. This invention can applied wide range in the field such as the immunity fluorescence mark, immunity histochemistry, biochemistry analysis, antibody test, protein chip and so on. It can replace the exist product efficiently, its operation is more simple, stability, delicacy and reliability, it is especially the same with the high flux analysis which analyze by the fluorescence analysis instrument.

Owner:SHANDONG UNIV AT WEIHAI

Methods for phenotyping of intact whole tissues

ActiveUS20160123854A1Reduced feature requirementsPreparing sample for investigationBiological testingWhole bodyTissue clearing

In various embodiments, the present application teaches methods and compositions for tissue clearing in which whole organs and bodies are rendered macromolecule-permeable and optically-transparent, thereby exposing their cellular structure with intact connectivity. In some embodiments, the present application teaches PACT, a protocol for passive tissue clearing and immunostaining of intact organs. In other embodiments, the present application teaches RIMS, a refractive index matching media for imaging thick tissue. In yet other embodiments, the application teaches PARS, a method for whole-body clearing and immunolabeling.

Methods for phenotyping of intact whole tissues

Methods for phenotyping of intact whole tissues

Methods for phenotyping of intact whole tissues

Owner:CALIFORNIA INST OF TECH

Methods for phenotyping of intact bones by tissue clearing and staining

InactiveUS20160131560A1Microbiological testing/measurementPreparing sample for investigationStainingTissue clearing

In various embodiments, the present application teaches methods and kits for clearing and optionally subsequently visualizing tissue containing bone. In some embodiments, the method includes serially incubating cleared bone in refractive index matching solutions with progressively higher refractive indexes. In some embodiments, the methods teach immunolabeling and/or staining tissue containing bone and optionally visualizing the immunolabeled and/or stained tissue containing bone.

Methods for phenotyping of intact bones by tissue clearing and staining

Methods for phenotyping of intact bones by tissue clearing and staining

Methods for phenotyping of intact bones by tissue clearing and staining

Owner:CALIFORNIA INST OF TECH

Preparation method and application of test strip for fast time-resolved fluorescent immunochromatography quantitative detection of amantadine

InactiveCN105301248ARapid detection of contentHigh sensitivityFluorescence/phosphorescenceCelluloseAntigen

The invention relates to a preparation method and application of a test strip for fast time-resolved fluorescent immunochromatography quantitative detection of amantadine. The test strip comprises three parts such as a Fusion 5 membrane, a cellulose nitrate membrane and water absorbent paper. According to the principle of the competition law, a time-resolved fluorescent microsphere is taken as an immune marker for accurate and fast quantitative detection of an antigen to be detected.

Preparation method and application of test strip for fast time-resolved fluorescent immunochromatography quantitative detection of amantadine

Preparation method and application of test strip for fast time-resolved fluorescent immunochromatography quantitative detection of amantadine

Preparation method and application of test strip for fast time-resolved fluorescent immunochromatography quantitative detection of amantadine

Owner:JIANGSU WISE SCI & TECH DEV

Apparatus and method for immunological labeling for thin tissue sections

InactiveUS20060128024A1Prevent evaporationEvaporation of the liquid droplets applied on the slide is thereby preventedBioreactor/fermenter combinationsBiological substance pretreatmentsTissue PartTissue sections

An apparatus and a method for immunological labeling of thin tissue sections are disclosed. The treatment fluid is applied in the form of liquid droplets onto at least one slide, the arrangement of liquid droplets corresponding to the arrangement of the thin tissue sections on the carrier plate. Each slide is retained in a transport container, the transport container being embodied in the form of a trough which comprises a peripheral delimiting wall that is closed off by a base. The base of one transport containers constitutes the cover of a transport container arranged beneath it. The transport containers are stacked in a first and a second station.

Apparatus and method for immunological labeling for thin tissue sections

Apparatus and method for immunological labeling for thin tissue sections

Apparatus and method for immunological labeling for thin tissue sections

Owner:LEICA MICROSYSTEMS GMBH

Silica fluorescent immunolabeled probe based on fluorescence resonance energy transfer as well as preparation method and application of silica fluorescent immunolabelled probe

PendingCN107976538AGood water solubilityEasy to retouchFluorescence/phosphorescenceCross-linkMesoporous silica

The invention provides a silica fluorescent immunolabeled probe based on fluorescence resonance energy transfer as well as a preparation method and application of the silica fluorescent immunolabelledprobe. The preparation method comprises the steps of using mesoporous silica nanoparticles as matrix, and constructing a fluorescence resonance energy transfer system (RB-BODIPY-FRET) by doping rhodamine B and BODIPY. The fluorescent labeling probe is coupled by using a sodium periodate oxidation antibody, so that a reliable basis is provided for the super-sensitive detection and immunoassay of nano-drug carriers. Compared with the prior art, the silica fluorescent immunolabeling probe is prepared by means of codoping of fluorescent dyes, i.e., the BODIPY and the rhodamine B, and fluorescenceresonance energy transfer occurs between the two fluorescent dyes, so that the fluorescence intensity is increased, and quenching is reduced; the fluorescent labeled probe is coupled by using the sodium periodate oxidation antibody, so that the coupling efficiency is improved, and the influence of a cross-linking agent on the activity of the antibody is eliminated.

Silica fluorescent immunolabeled probe based on fluorescence resonance energy transfer as well as preparation method and application of silica fluorescent immunolabelled probe

Silica fluorescent immunolabeled probe based on fluorescence resonance energy transfer as well as preparation method and application of silica fluorescent immunolabelled probe

Silica fluorescent immunolabeled probe based on fluorescence resonance energy transfer as well as preparation method and application of silica fluorescent immunolabelled probe

Owner:ANHUI NORMAL UNIV

Biotinylated liposome used for detecting pancreas cancer marker REG1A, and preparation method and applications thereof

InactiveCN107674884AImprove featuresGood choiceMicroencapsulation basedMicrobiological testing/measurementPancreas CancersCholesterol

The invention relates to a biotinylated liposome used for detecting pancreas cancer marker REG1A. The biotinylated liposome used for detecting pancreas cancer marker REG1A comprises coated reporter DNA fragments, and PEG-200 surface modified biotin; according to the preparation method, DPPC, cholesterol, DSPE-PEG2000, DSPE-PEG2000-biotin film dispersion method is adopted to prepare the biotinylated liposome used for coating reporter DNA fragments, and the biotinylated liposome is taken as an immunolabelling biosensor. The invention also relates to a method used for detecting pancreas cancer marker REG1A based on the biotinylated liposome. The method comprises following steps: an ELSA plate is coated with REG1A murine monoclonal antibodies, a REG1A protein sample to be detected is added, reactions with REG1A rabbit source polyclonal antibodies, biotinylated goat anti rabbit IgG, and avidin are carried out respectively, at last the biotinylated liposome of an optimized concentration is added, fluorescence quantitative LAMP amplification is carried out, and the concentration of the REG1A protein is calculated based on amplification results. According to the preparation method, immunization LAMP reaction is combined with immunoliposome nano-particles, so that high sensitivity detection of pancreas cancer marker REG1A is realized, stability is high, cost is low, and the specificityis high.

Biotinylated liposome used for detecting pancreas cancer marker REG1A, and preparation method and applications thereof

Biotinylated liposome used for detecting pancreas cancer marker REG1A, and preparation method and applications thereof

Biotinylated liposome used for detecting pancreas cancer marker REG1A, and preparation method and applications thereof

Owner:SHANGHAI IGENETEC DIAGNOSTICS CO LTD +1

Quantitative in situ characterization of heterogeneity in biological samples

ActiveUS9613254B1Microscopic object acquisitionMaterial analysisImmune markersStromal region

The present disclosure relates to characterization of biological samples. By way of example, a biological sample may be contacted with a plurality of probes specific for targets in the sample, such as probes for immune markers and segmenting probes. Acquired image data of the sample may be used to segment the images into epithelial and stromal regions to characterize individual cells in the sample based on the binding of the probes. Further, the biological sample may be characterized by a heterogeneity of the characterized cells.

Quantitative in situ characterization of heterogeneity in biological samples

Quantitative in situ characterization of heterogeneity in biological samples

Quantitative in situ characterization of heterogeneity in biological samples

Owner:LEICA MICROSYSTEMS CMS GMBH

Method to detect virus related immunological markers for the diagnosis of respiratory tract infections

InactiveUS20100021882A1Bioreactor/fermenter combinationsSequential/parallel process reactionsAntigenSerum samples

This invention discloses using SPR technology to simultaneously and qualitatively detect the presence of respiratory tract viruses-related immunological markers in a serum sample, which can be used for the diagnosis of respiratory tract infections. It also discloses an efficient formula to make a mixed SAM that can greatly enhance the immobilization ability of the metal surface in SPR based techniques, which is good for the immobilization of representative antigens used to detect the respective respiratory tract viruses-related immunological markers (antibodies) in blood for the diagnosis of respiratory tract infections.

Owner:CMED TECH

Test strip for testing chloramphenicol by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing

InactiveCN109813900ARapid detection of contentHigh sensitivityBiological testingAntigenFluorescence

The invention relates to a preparation method and application of a quick test strip for testing chloramphenicol by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing. The test strip is composed of a Fusion5 membrane, a nitrocellulose membrane and water absorption paper, according to the competition law principle, time-resolved fluorescence microspheres are taken as an immune marker, and accurate and quick quantitative testing can be conducted on a to-be-tested antigen.

Test strip for testing chloramphenicol by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing

Test strip for testing chloramphenicol by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing

Test strip for testing chloramphenicol by adopting time-resolved fluorescence lateral flow immunoassay quantitative testing

Owner:NANJING YITE BIOTECH

Detection kit for preeclampsia of pregnant women

InactiveCN107884570AAvoid discomfortSimple detection operationBiological testingBiologyTest strips

The invention relates to a detection kit for preeclampsia of pregnant women. The kit comprises PIGF and a sFlt-1 immunochromatography detection test strip or a PIGF immunochromatography detection teststrip and an sFlt-1 immunochromatography detection test strip, relatively preferably, a PIGF detecting immunolabelling antibody and a PIGF detecting antibody are separately arranged on a combining pad and a nitrocellulose membrane of the PIGF immunochromatography detection test strip; an sFlt-1 detecting immunolabelling antibody and an sFlt-1 detection antibody are separately arranged on a combining pad and a nitrocellulose membrane of the sFlt-1 immunochromatography detection test strip; or, the immunolabelling antibodies and the antibodies are separately coated to the combining pads and thenitrocellulose membranes of the PIGF and sFlt-1 immunochromatography detection test strips. According to the kit provided by the invention, PIGF and sFlt-1 can be detected at the same time by sampling once, so that the kit is simple to apply, and can acquire the ratio of the two accurately to further predict the onset risk of preeclampsia of pregnant women accurately, thereby providing an effective basis for accurate treatment. The kit is suitable for being popularized and applied on a large scale.

Detection kit for preeclampsia of pregnant women

Detection kit for preeclampsia of pregnant women

Detection kit for preeclampsia of pregnant women

Owner:江苏龙维生物科技有限公司

Group A streptococcus antigen detection test strip and kit and preparation methods thereof

InactiveCN110568187AReduce distractionsRule out false positivesMaterial analysisAntigen testImmunolabeling

The invention discloses a group A streptococcus antigen detection test strip and kit and preparation methods thereof, and relates to the technical field of group A streptococcus detection. The group Astreptococcus antigen test strip is prepared by sequentially attaching a nitrocellulose membrane coated with an anti-group A streptococcus detection antibody, an immune binding pad coated with an anti-group A streptococcus immunolabeled antibody, absorbent paper and a sample pad to a polyvinyl chloride bottom plate; a detection card can detect whether a group A streptococcus antigen is present ina sample to be detected or not by detecting a marker; and the test strip and the detection card comprising the test strip can specifically and quickly detect and diagnose early infection of group A streptococcus, thereby reducing the cost, realizing rapid detection and meeting the requirements of clinical use.

Group A streptococcus antigen detection test strip and kit and preparation methods thereof

Group A streptococcus antigen detection test strip and kit and preparation methods thereof

Group A streptococcus antigen detection test strip and kit and preparation methods thereof

Owner:JIANGSU BIOPERFECTUS TECH CO LTD

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