3 results about "Cells/well" patented technology

The invention discloses a cell adherence promoting agent, a method for realizing rapid cell planking by using the cell adherence promoting agent and application of the cell adherence promoting agent. The rapid planking method comprises the following steps: pretreating a cell pore plate by using the adherence promoting agent, adding target cells obtained by resuscitation and resuspension into the pretreated cell pore plate, and incubating in a water bath at 35-37 DEG C for 1-1.2 hours to finish planking; the adhesion promoting agent is sericin. By adopting the method, the adherence rate of the target cell GABABR-HEK293T can reach 80% or above, even a pore plate treated by 1mu g / mL sericin is adopted, the cell does not fall off, the complete adherence is achieved, the total time is about 2.5 hours when the method is used for CBA detection, the time is consumed by 121 hours compared with the conventional CBA detection experiment process, the time is saved by nearly 5 days, and the labor and time cost is greatly reduced.

The invention belongs to the technical field of medical immunodetection analysis, and discloses a kit for detecting an anti-AQP4 autoantibody and application of the kit. The kit comprises a cell pore plate and a cell freezing medium; reaction holes of the cell pore plate are internally coated with a coating substance, and the coating substance is one of polylysine, gelatin and collagen; the cell cryopreservation solution is a cryopreservation protection solution of cells containing overexpressed AQP4 antigens; during coating, the concentration of a coating substance is 0.5 mg / mL-3. 0 mg / mL, and the dosage of the coating substance is 40 [mu] L / hole to 60 [mu] L / hole. According to the kit for detecting the anti-AQP4 autoantibody, the detection performance can be improved, and the kit can be frozen at low temperature for a long time and then unfrozen for detection.

The utility model provides a kind of cell well plate, from bottom to top sequentially include substrate, middle layer, hydrophobic layer, the middle layer is equipped with a plurality of sample wells, the sample well is communicated with the substrate, electrode is provided on the substrate, and the electrode is located the sample well below;The electrode is used to generate uneven electric field, so that cell or bacteria is inclined to the side movement of uneven electric field and is settled in the bottom of the sample well;The middle layer is used to clean sample liquid;The hydrophobic layer is used to keep sample in the sample well and sample well between not connected and flows in series.The utility model adds electric field below well plate, improves cell settling velocity by dielectrophoresis force effect, and cell (or bacteria) is not easy to lose under the action of dielectrophoresis force after settling, and has better retention rate in laminar flow cleaning process.