2 results about "Cells/well" patented technology

The invention belongs to the technical field of medical immunodetection analysis, and discloses a kit for detecting an anti-AQP4 autoantibody and application of the kit. The kit comprises a cell pore plate and a cell freezing medium; reaction holes of the cell pore plate are internally coated with a coating substance, and the coating substance is one of polylysine, gelatin and collagen; the cell cryopreservation solution is a cryopreservation protection solution of cells containing overexpressed AQP4 antigens; during coating, the concentration of a coating substance is 0.5 mg / mL-3. 0 mg / mL, and the dosage of the coating substance is 40 [mu] L / hole to 60 [mu] L / hole. According to the kit for detecting the anti-AQP4 autoantibody, the detection performance can be improved, and the kit can be frozen at low temperature for a long time and then unfrozen for detection.

The utility model provides a kind of cell well plate, from bottom to top sequentially include substrate, middle layer, hydrophobic layer, the middle layer is equipped with a plurality of sample wells, the sample well is communicated with the substrate, electrode is provided on the substrate, and the electrode is located the sample well below;The electrode is used to generate uneven electric field, so that cell or bacteria is inclined to the side movement of uneven electric field and is settled in the bottom of the sample well;The middle layer is used to clean sample liquid;The hydrophobic layer is used to keep sample in the sample well and sample well between not connected and flows in series.The utility model adds electric field below well plate, improves cell settling velocity by dielectrophoresis force effect, and cell (or bacteria) is not easy to lose under the action of dielectrophoresis force after settling, and has better retention rate in laminar flow cleaning process.