Method for extracting hesperidin in dried tangerine or orange peel by using enzymolysis approach
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A technology of hesperidin and tangerine peel, applied in the field of medicine, can solve the problems of limited application, high noise of ultrasonic method, and radiation of microwave method
Inactive Publication Date: 2015-04-29
JINHUA VOCATIONAL TECH COLLEGE
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Problems solved by technology
Although the extraction time of these two methods is short, the ultrasonic method is very noisy, and the microwave method has radiation. They have high requirements for extraction equipment, which limits their application in large-scale production of enterprises to a certain extent.
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Embodiment 1
[0009] 1. Extraction of hesperidin:
[0010] The tangerine peel medicinal material is crushed into a coarse powder, weighed 100.00g, added 300mL distilled water, added pectinase (300000U / g), the dosage is 0.3% of the medicinal material quality, the pH value of the solution is adjusted to 3 with dilute hydrochloric acid, and placed in 45 ℃ in a constant temperature water bath for 2 hours, take it out, filter, concentrate the filtrate to an extract, and then place it in a vacuum oven and dry it into powder to obtain the hesperidin extract.
[0011] 2. Determination of the content of hesperidin in the extract
[0012] The HPLC method is adopted for determination, and the determination method refers to the method for measuring the content of hesperidin in tangerine peel medicinal materials of the 2010 edition of "Chinese Pharmacopoeia".
[0013] (1) Preparation of standard solution Accurately weigh 10.1 mg of standard hesperidin, add an appropriate amount of methanol, dissolve in...
Embodiment 2
[0021] 1. Extraction of hesperidin:
[0022] Weigh 100g of dried tangerine peel medicinal material coarse powder, add 200mL distilled water, add pectinase (30000U / g), the dosage is 3% of the medicinal material quality, adjust the pH value of the solution to 4 with dilute hydrochloric acid, and place it in a constant temperature water bath at 45°C After enzymolysis for 1.5 hours, take it out, filter, concentrate the filtrate to an extract, and then place it in a vacuum drying oven and dry it into powder to obtain the hesperidin extract.
[0023] 2. Determination of Hesperidin Extraction Rate
[0024] According to the above HPLC method, 5.02 g of hesperidin was measured in the hesperidin extract, and the extraction rate of hesperidin was 5.02%.
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Abstract
The invention provides a method for extracting hesperidin in dried tangerine or orange peel by using an enzymolysis approach and relates to the field of medicines. Because citrus and other peels are enriched in pectin, and dissolution of active ingredients in cells is hindered by the pectin. According to the method disclosed by the invention, the pectin between cell walls of medicinal materials and cells is subjected to enzymolysis by adopting pectinase, so that the cell wells are destroyed, the hesperidin is fully dissolved, and the aim of obviously improving the hesperidin extraction rate is achieved. The method can be used for extracting the hesperidin in medicinal materials such as the dried tangerine or orange peel, fructus aurantii and immature bitter orange. The method disclosed by the invention has the advantages that the operation is simple, organic solvent residues are avoided, the environmental pollution is avoided, special equipment is not needed, the production safety is high, large-scale enterprise production is promoted, and the like.
Description
technical field [0001] The invention provides a method for extracting hesperidin by enzymatic hydrolysis, and relates to the field of medicine. Background technique [0002] Hesperidin has the functions of reducing vascular fragility, lowering blood pressure, anti-allergy, and inhibiting tumors. It is clinically used as an adjuvant treatment for cardiovascular system diseases, and can also be used as a raw material for pharmaceuticals in the pharmaceutical industry. Hesperidin is also used as a natural antioxidant Used in the food and cosmetic industry. [0003] The traditional methods of extracting hesperidin from citrus peels are hot water extraction, alcohol reflux extraction, alkali extraction and acid precipitation. These methods all use chemical solvents to infiltrate and dissolve the active ingredients in the medicinal materials. Although they are simple and easy to operate, they have shortcomings such as incomplete extraction, causing some hesperidin to remain in th...
Claims
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