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Method for obtaining high-purity proteins from wild nettles

A nettle protein, high-purity technology, applied in the preparation method of peptides, chemical instruments and methods, plant peptides, etc., can solve the problems of low yield, high production cost, low yield and purity of protein products, and achieve extraction time short effect

Active Publication Date: 2015-04-22
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protein produced by this method is not only low in product yield and purity, but also easily causes a large amount of protein decomposition and denaturation, resulting in low yield and high production cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Crush the wild nettle with a pulverizer to grind the mesh between 20-40 mesh, then use petroleum ether to degreasing, degreasing 2 times, 1 hour each time, degreasing temperature 50 ± 2 ℃; put the degreasing raw materials into In the ultra-high pressure extraction tank, use alkaline water with a pH of 8.0 as the extraction solvent, extract at room temperature, start the high-pressure pump, and quickly increase the pressure to 100 MPa after exhausting the air in the container. Maintain this pressure for 18 minutes, and then quickly Open the valve that controls the high-pressure circuit, release the pressure, and release the extract. Centrifuge the extract and add an acidic aqueous solution to the centrifuge to make the pH of the centrifuge at 4.5. After stirring, let it stand for 60 minutes. Use a horizontal unloading centrifuge for solid-liquid separation; use 35℃ warm water for the solid Perform multiple rinses until the pH is neutral. The washed protein was freeze-dri...

Embodiment 2

[0018] Crush the wild nettle with a pulverizer to grind the mesh between 20-40 mesh, then use n-hexane to degreasing, degreasing 2 times, 1 hour each time, degreasing temperature 50 ± 2 ℃; put the degreased raw materials In the ultra-high pressure extraction tank, use alkaline water with a pH of 8.5 as the extraction solvent, extract at room temperature, start the high-pressure pump, and quickly increase the pressure to 105MPa after exhausting the air in the container, maintain this pressure for 20 minutes, and then quickly Open the valve that controls the high-pressure circuit, release the pressure, and release the extract. Centrifuge the extract and add an acidic aqueous solution to the centrifuge to make the pH of the centrifuge at 4.6. After stirring, let it stand for 30 min. Use a butterfly centrifuge for solid-liquid separation; use 30°C warm water for more solids. Rinse once until the pH is neutral. The washed protein was freeze-dried. The freeze-drying temperature was ...

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Abstract

The invention belongs to the field of natural organic chemistry, relates to a method for extracting and separating high-purity plant proteins from nettles, and in particular relates to a method for extracting the high-purity plant proteins by utilizing a superhigh pressure technique. The method has the advantages that low-temperature or constant-temperature extraction is carried out by utilizing the superhigh pressure technique, so that the maintenance of the plant protein activity is facilitated; extracted solvents can flow into the plant cells well by utilizing the superhigh pressure technique, so that high extraction efficiency of the plant proteins is realized; the extraction time is short and the energy consumption is low; the content of impurities is reduced, and the protein purity is high; the extraction process is simple and environmental-friendly. According to the method, the nettles used as the raw materials are degreased and extracted by using alkali water as an extraction solvent under the superhigh pressure condition, and the extracting liquid is subjected to centrifuge, acid sediment, washing, drying and the like to obtain the high-purity plant proteins.

Description

Technical field [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for preparing high-purity vegetable protein by using wild nettle in Daxinganling as a raw material. Background technique [0002] Nettle is a perennial herb, a shade-loving plant, with vigorous life and rapid growth. It does not require strict soil requirements. It grows on hillsides, roadsides or in semi-shady wet places next to houses. There are many under-forest plants in the Greater Xing'an Mountains in my country. According to authoritative departments, tens of millions of tons of nettles grow under the woods every year. They are one of the main plant species in the Greater Xing'an Mountains. The protein content of wild nettles is not high compared to beans, but due to the rich resources of wild nettles in my country, the reserves are huge. Therefore, deep processing and development of nettle protein products have huge economic and social benefits. [0003] Nettle ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/14
CPCC07K14/415
Inventor 苏学辉姚德坤刘巍
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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