A set of Sinkiang drug mulberry tissue culture and rapid propagation culture medium comprises the following components: a germination culture medium, a multiplication culture medium, an extension culture medium and a root culture medium, wherein the germination culture medium is prepared from the following raw materials: MS 4.33 g/L, 6-BA 2.0 mg/L, NAA 0.2 mg/L, sugar 30 g/L and agar 7 g/L, and the pH value of the germination culture medium is 5.8; the multiplication culture medium is prepared from the following raw materials: MS 4.33 g/L, 6-BA 1.0 mg/L, sugar 30 g/L and agar 7 g/L, and the pH value of the multiplication culture medium is 5.8; the extension culture medium is prepared from the following raw materials: MS 4.33 g/L, ZT 4.5 micro-mol/L, sugar 30 g/L and agar 7 g/L, and the pH value of the extension culture medium is 5.8; and the root culture medium is prepared from the following raw materials: WPS 2.30 g/L, L+NAA 0.2 mg/L, IBA 0.2 mg/L, activated carbon 0.4 g/L, sugar 20 g/L and agar 6 g/L, and the pH value of the root culture medium is 6.0. The culture medium is simple and convenient to prepare, and is high in multiplication efficiency, each seedling can differentiate 2.3 lateral buds averagely, the transplanted seedlings are got by winter buds after four months, and the survival rate after transplantation is high to more than 75%.