9results about How to "Improve proliferation efficiency" patented technology

The invention discloses a serum substitute compatible umbilical cord mesenchymal stem cell culture medium additive composition and application thereof. The serum substitute compatible umbilical cord mesenchymal stem cell culture medium additive composition is prepared from the following components in parts by mass: 15 to 25 parts of platelet-derived growth factor-BB; 15 to 25 parts of basic fibroblast growth factors; 8 to 12 parts of transforming growth factor-beta; and 8-12 parts of recombinant human insulin. The serum substitute compatible umbilical cord mesenchymal stem cell culture medium additive composition and the application thereof disclosed by the invention have the following beneficial effects: (1) the proliferation efficiency is remarkably improved; (2) the cell morphology and dryness can be well maintained; and (3) the compliance and the compatibility are good.

The application relates to a microbial fermentation device for beef jerky processing and relates to the technical field of food fermentation equipment, and comprises a material conveying pipe. The application can accurately control the temperatures of the front section, the middle section and the rear section of the material conveying pipe by arranging three groups of heating components and uniformly distributed temperature sensors, can match the growth activity peak value of probiotics, can improve the proliferation efficiency of a bacterial population and the activity of probiotics, can avoid flavor substance imbalance, and can improve the heating efficiency and uniformity by adopting an opening design of a heat preservation shell and a spiral-wound heating pipe. Meanwhile, the heat loss is reduced by a protective shell and a thermal insulation aerogel, the internal components are protected, the perforation of spiral conveying blades is matched with a turnover plate, the beef jerky material mixing is promoted, the temperature accurate control in the beef jerky fermentation process, the uniform mixing of beef jerky material and the uniform distribution of bacterial liquid are realized, the fermentation efficiency and product flavor coordination are effectively improved, the fermentation period is shortened, and the product quality is improved.

The present application relates to the fields of animal nutrition and microbial fermentation technology, and particularly relates to a strain of Saccharomyces boulardii, a microecological preparation thereof and application thereof.The Saccharomyces boulardii is named as NCUTY 002, has been preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 2025648.The Saccharomyces boulardii NCUTY 002 screened in the present application has excellent acid tolerance (pH≤2.5) and bile salt tolerance (0.5%), and shows high self-aggregation capacity, so that it can survive and stably colonize in the extreme environment of digestive tract of egg ducks, and can be used as a special feed additive for egg ducks.

The application discloses a kind of human mesenchymal stem cell 3D culture and amplification method, belong to stem cell culture amplification technical field.The application is prepared 3D cell culture matrix by structure design and component optimization, realize the accurate simulation of stem cell in-vivo microenvironment.The matrix is modified sodium alginate by amino hexene acid and is compounded with methacryl gelatin, and is crosslinked by lithium chloride and calcium chloride, and is synergistically formed by photocrosslinking, and the three-dimensional network has uniform pore size and excellent connectivity.The application significantly improves cell adhesion rate and proliferation efficiency, effectively maintains the biological characteristics of hMSC such as immune regulation and differentiation, the degradation rate of the matrix is dynamically matched with the cell proliferation cycle, the material has excellent biocompatibility and the degradation product is non-toxic, which solves the defects of traditional 2D culture and existing 3D technology, and provides an efficient and safe technical solution for hMSC scale-up and clinical translation.

The present application relates to stem cell technology, in particular to a pet stem cell induction medium, a preparation method and a culture method, the medium significantly improves the proliferation efficiency and multi-directional differentiation potential of adipose-derived stem cells by optimizing the ratio of specific growth factors. The preparation method comprises the steps of preparing a basic medium, adding growth factors and sterile filtering. Experimental verification shows that compared with the control group, the adipogenic differentiation efficiency of ADSCs cultured in the medium is increased by 40%, the osteogenic differentiation ALP activity is increased by 65%, and the positive rates of surface markers CD29, CD44 and CD90 are all greater than or equal to 95%. The present application solves the problems of animal source pathogen contamination risk and low induction efficiency of traditional culture medium, and is suitable for pet medical treatment.

The present application relates to a kind of preparation method for preparing neural stem cell with high proliferative capacity, belong to the field of biotechnology.The method is isolated from mammalian embryo brain tissue primary neural stem cell, with DMEM / F12 as base medium, add B27, N2, glutamine and double resistance, then join epidermal growth factor, basic fibroblast growth factor, insulin-like growth factor-1, Y-27632 and heparin form optimized proliferation culture medium.Cell is seeded in the culture dish coated with polylysine and laminin, culture under suitable conditions, neural ball reaches certain diameter after digestion and passage.This method can improve the proliferation efficiency of neural stem cell, maintain stemness and differentiation potential, adopt serum-free system, simple operation, suitable for large-scale expansion and research application.

This invention discloses the application of a polysaccharide coating membrane in the preparation of cell culture products, belonging to the field of cell culture food technology. Specifically, after cells are seeded onto a scaffold, a polysaccharide coating membrane is formed around it. This can alleviate the phenomenon of cells detaching from the scaffold during culture, thereby ensuring the effective proliferation and differentiation of muscle stem cells and / or adipose stem cells on the scaffold, providing a new solution for the efficient and standardized production of cell culture products.

The application discloses a tissue culture large-scale propagation method of Camellia meiocarpa. The method comprises the following steps: obtaining aseptic seedlings of Camellia meiocarpa, cluster bud proliferation culture, single plantlet culture of adventitious buds, bottle-out root preparation culture and bottle-out root culture. The composition of the cluster bud proliferation culture medium and the plantlet culture medium is optimized, the cluster bud proliferation and the formation of the adventitious buds are promoted, and the proliferation coefficient reaches 8.18. In addition, the bottle-in and bottle-out root culture scheme is combined, so that the rooting rate and the survival rate are significantly improved, and the rooting rate reaches 90%. The tissue culture method has important significance in the protection and population recovery of Camellia meiocarpa, and provides technical support for the protection and sustainable utilization of the endangered species.

The invention relates to the technical field of biological fermentation, and particularly discloses a method for preparing L-alanine through biomass fermentation. The method comprises the following steps: by taking corncobs, corns or corn straws as raw materials, sequentially carrying out acid treatment, alkali treatment and enzymolysis to obtain saccharified liquid rich in glucose, and taking the saccharified liquid as a fermentation carbon source; then carrying out seed culture on the L-alanine production strain, and carrying out immobilized packaging by adopting a sodium alginate-chitosan system to prepare an immobilized cell microcapsule; finally, the microcapsules are inoculated into a fermentation culture medium with corncob saccharification liquid as a main material for strict anaerobic fermentation, and low residual sugar concentration is maintained by feeding the saccharification liquid. According to the method, efficient saccharification and comprehensive utilization of the corn biomass are achieved, the stability and reusability of thalli in the production environment are improved through the cell immobilization technology, the production efficiency and product concentration of L-alanine are remarkably improved, and the method has the advantages of being low in raw material cost and environmentally friendly in process.