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Method for establishing new lycoris variety 'Taohong' tissue culture rapid-propagation technical system

A technology for tissue culture rapid propagation and establishment method, which is applied in the field of establishment of a tissue culture rapid propagation technology system of a new variety 'Taohong' of the genus Lycoris, can solve the problems of poor fertility, lack of fruit, slow speed and the like, and achieves improvement of the reproduction coefficient. and the effect of reproduction speed

Inactive Publication Date: 2019-12-20
HANGZHOU BOTANICAL GARDEN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the origin of hybridization, the fertility is poor and there is almost no fruit; natural division of bulbs, slow speed, and low reproduction coefficient; an average flowering bulb produces 1-2 seed bulbs every year, and it takes about 3-4 years for the seed bulbs to grow and bloom. time

Method used

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  • Method for establishing new lycoris variety 'Taohong' tissue culture rapid-propagation technical system
  • Method for establishing new lycoris variety 'Taohong' tissue culture rapid-propagation technical system

Examples

Experimental program
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Effect test

Embodiment 1

[0020] The tissue culture rapid propagation of embodiment 1' Taohong'

[0021] (1) Preparation of explants: In September 2018, take a 3-year-old healthy 'Peach Red' bulb with a diameter of about 5 cm, peel off the outer scale leaves (peel off directly by hand), cut off all roots, 1 / 3 Bulb disk and 2 / 3 bulbs, keep the bulb disk, wash the soil on the surface with a brush, cut into 4 pieces according to the size of the bulb disk, each piece is 0.5cm in size 2 , 84 detergent shock wash for 30 minutes, rinse with running water for 30 minutes. Then the above-mentioned materials were transferred to the ultra-clean workbench and rinsed with alcohol with a volume concentration of 75% for 30s, and a mass concentration of 0.1% HgCl 2 Sterilize for 40 minutes, rinse with sterile water 4-5 times, absorb the moisture on the surface with sterile absorbent paper, and set aside.

[0022] (2) Induction of adventitious buds The sterilized bulbs are directly inoculated on the adventitious bud i...

Embodiment 2

[0028] Tissue Culture and Rapid Propagation of 'Taohong'

[0029] (1) Preparation of explants: In September 2018, take a 3-year-old healthy 'Peach Red' bulb with a diameter of about 5 cm, peel off the outer scale leaves (peel off directly by hand), cut off all roots, 1 / 3 Bulb disk and 2 / 3 bulbs, keep the bulb disk, wash the soil on the surface with a brush, cut into 4 pieces according to the size of the bulb disk, each piece is 0.5cm in size 2 , 84 detergent shock wash for 30 minutes, rinse with running water for 30 minutes. Then the above-mentioned materials were transferred to the ultra-clean workbench and rinsed with alcohol with a volume concentration of 75% for 30s, and a mass concentration of 0.1% HgCl 2 Sterilize for 40 minutes, rinse with sterile water 4-5 times, absorb the moisture on the surface with sterile absorbent paper, and set aside.

[0030] (2) Induction of adventitious buds The sterilized bulbs are directly inoculated on the adventitious bud induction medi...

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Abstract

The invention discloses a method for establishing a new lycoris variety 'Taohong' tissue culture rapid-propagation technical system. According to the method, bulb blocks are taken as explants, the explants are disinfected, and the 'Taohong' tissue culture rapid-propagation technical system is established after adventitious bud induction, cluster bud induction, bud hardening, seedling hardening androoting culture, seedling hardening and transplanting steps. Compared with other original species of lycoris, when the rapid-propagation technology system is adopted to cultivate a new lycoris variety 'Taohong', the characteristic is utilized that the new lycoris variety is more resistant to high-concentration plant growth regulators, and the high-concentration plant growth regulators are used atbud induction and bud proliferation stages so that a bud germination period can be shortened, and the bud proliferation efficiency can be improved. By adopting the tissue culture rapid-propagation technology, the propagation coefficient and propagation speed of the new lycoris variety can be greatly improved, and a technical foundation is laid for application and popularization of the new lycorisvariety.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for establishing a tissue culture rapid propagation technology system of a new species of Lycoris genus 'Taohong'. Background technique [0002] Lycoris Herb. belongs to Amaryllidaceae (Amaryllidaceae), a perennial herb. Bulbs ovoid, leaves in spring or autumn, ribbon-shaped or sword-shaped leaves; dormancy in late spring or early summer; flowering period from July to September, umbels with 4-6 small flowers; flowers yellow, red, white. The flower stems are tall and straight, the flower shape is beautiful, and the flower color is gorgeous. All species of this genus have high ornamental value, and have gradually become new-type fresh-cut flowers, garden ground covers and potted bulbous flowers. [0003] 'Taohong' is a new variety of Amaryllis selected by Hangzhou Botanical Garden through cross breeding. Due to the origin of hybridization, the fertility ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张鹏翀郑玉红鲍淳松
Owner HANGZHOU BOTANICAL GARDEN
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