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Quick breeding method for tissue culture of lemon verbena

A lemon verbena, tissue culture technology, applied in horticultural methods, botanical equipment and methods, gardening and other directions, can solve the problems of difficulty in seed reproduction of lemon verbena, low survival rate of cutting propagation, etc., and achieves suppression of culture medium and explantation The effect of body browning, improved disinfection effect and low survival rate

Inactive Publication Date: 2007-07-11
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to solve the problem of difficult propagation of lemon verbena seeds and low survival rate of cutting propagation, to provide a method for rapid propagation of lemon verbena through tissue culture, improve the reproductive coefficient of lemon verbena, and ensure the genetic stability of lemon verbena

Method used

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Embodiment Construction

[0019] The technical solution of the present invention will be further described below through specific embodiments.

[0020] 1. Sterilize the explants to remove the young branches of lemon verbena from the leaves, soak them in detergent water for 20 minutes, then rinse with tap water for 2 hours, drain them, put them in 70% alcohol and stir for 15 seconds, remove and turn Soak in a saturated bleach solution containing Tween-80 for 10 minutes. During this time, use a soft brush to brush the material, especially the axillary buds, and then take it out on a clean bench, rinse with sterile water for 3 to 4 times, and then use it. Sterilize absorbent paper to absorb the water, cut the young and nodular stem segments at the lower end of the apical buds, quickly inoculate them on MS medium, place them in the dark for cultivation, and transfer them to new MS medium on the next day.

[0021] 2. Inducing axillary buds, transfer the uncontaminated and browned stems into the medium MS+6-BA1....

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Abstract

A tissue culture method for fast reproduction of limonvervain includes such steps as providing the young stem with nodes of limonvervain as explant, brushing the sterilizing liquid on it (especially on the axiallary buds), inoculating, culturing in different culture media in darkness, culturing sequentially in inducing culture medium, reproductive culture medium and rooting culture medium to obtain test-tube plantlet, transplanting it on the bed made of vermiculite and humus, and transplanting it in field.

Description

Technical field [0001] The invention relates to a method for rapid propagation of lemon verbena in tissue culture, more specifically, the invention relates to a method for inducing tissue culture of axillary buds to rapidly propagate lemon verbena. Background technique [0002] The lemon verbena grassland is produced in Chile, Argentina and Peru in tropical South America, and is also cultivated in Morocco in North Africa. In our country, only a few plants were introduced from Japan and planted in the greenhouse of this experimental group. It is rich in volatile oils, and the extracted essential oils can be used in cosmetics and have high medicinal value. Because of its unique lemon fragrance, it is favored by people and is widely used in food, tea and beverages; and because of its leaf color, The leaf shape is beautiful, and it can also be used as a planting material for garden greening and air purification. This not only increases the demand for lemon verbena, but also doubles i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张艳玲姚雷
Owner SHANGHAI JIAOTONG UNIV
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