Method for quickly propagating leeka through tissue culture

Abstract

The invention discloses a method for quickly propagating leeks through tissue culture. The method for quickly propagating the leeks through the tissue culture comprises the following steps: firstly, inoculating a sterilized seed onto a 1 / 2MS solid culture medium, and culturing the sterilized seed to obtain a sterile radicle under a certain condition; secondly, cutting the radicle tip, and inoculating the radicle tip onto a culture medium of MS+2mg / L6-BA+1mg / LNAA; thirdly, inducing to form a callus, and inoculating the callus onto a culture medium of MS+1mg / L6-BA+0.5mg / LNAA+0.5mg / LKT; fourthly, promoting differentiation of adventitious buds, and cutting the callus with the adventitious buds into small pieces with as less buds as possible, inoculating the small pieces onto a culture medium of MS+1mg / LGA+0.5mg / LKT, and growing the adventitious buds into independent leek seedlings; and finally, transferring the independent leek seedlings to a culture medium of 1 / 2MS+0.2mg / LIAA, performing rooting culture, hardening the leak seedlings and transplanting the leek seedlings into soil. Compared with the prior art, the method has the advantages as follows: the callus inducing rate is improved by more than 20%, the bud inducing rate is improved by more than 30%, and the propagation coefficient and the survival rate are improved by more than 15% and 10% respectively; and by the method, the variety propagating time is shortened, and a foundation is laid for improving a variety through genetic transformation.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a high-efficiency and rapid propagation method for leek tissue culture by using root explants. Background technique [0002] Chinese chives( Allium tuberosum Rottl. Ex Spreng), also known as stalactites and sunflowers, belongs to the Liliaceae Allium genus. It is a perennial herbaceous plant. It mainly feeds on leaves and pseudostems. It has a special and strong chive smell. Vegetable varieties unique to our country. Leeks are rich in vitamin C, carbohydrates, sulfur, phosphorus, iron and other minerals. Leek is not only rich in nutrition, but also has many health care functions. Its unique volatile aroma can promote appetite; the allicin and sulfide contained in it have excitatory effects on the human body and have a bactericidal effect in the intestinal tract; rich in carotene , has curative effect on night blindness and cracked skin; and dietary fiber can signi...

AI Technical Summary

Problems solved by technology

At present, in the tissue culture research of Chinese chives, the in vitro propagation of Chinese chives can be carried out through stem tips, immature embryos and unfertilized ovaries, but the efficiency is not very high

In the study of root explants, Shuto and Zhang Song both induced callus through the root tip, and then differentiated into plants. Later, there were some related reports, but there was a big difference in the use of hormone formula, and the research results were not systematic. , photos of clustered buds can be seen, but no photos of individual plants with pseudostems