A kind of method for tissue culture of Hemerocallis grandiflora leaf

A technology for tissue culture of Hemerocallis Hemerocallis, applied in the field of plant tissue culture, can solve the problems of reproductive impact, fewer varieties of Hemerocallis Hemerocallis, restriction on the cultivation of new varieties and fine varieties, etc. The effect of short tissue culture time

Inactive Publication Date: 2018-04-24
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few varieties of Hemerocallis grandiflora in garden applications at present, the main reason is that it is affected by reproduction.
Hemerocallis grandiflora in garden applications is mainly propagated through tissue culture. The explants commonly used in tissue culture include scapes, petals, ovaries, and stem tips. Breeding of varieties

Method used

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  • A kind of method for tissue culture of Hemerocallis grandiflora leaf
  • A kind of method for tissue culture of Hemerocallis grandiflora leaf
  • A kind of method for tissue culture of Hemerocallis grandiflora leaf

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Embodiment 1

[0031] Embodiment 1 Hemerocallis grandiflora leaf tissue culture

[0032] In this example, a new Hemerocallis variety "Red Triangle" was selected for leaf tissue culture.

[0033] 1) Selection and disinfection of explants: select a single plant of Hemerocallis grandiflora with a large number of new leaves, select new leaves with a leaf age of 9-10 days, and intercept the young leaves above the stem tip to ensure as much as possible Consistency of explants. Rinse the explants with running water for 3-5 minutes, then sterilize the surface with 70% alcohol for 30 seconds, rinse with sterile water three times, then soak in 2% NaClO for 10-15 minutes, and then rinse with sterile water for 5-6 times.

[0034] 2) Induction of callus: In the ultra-clean workbench, the leaves treated in step 1) were torn into long squares of 1 cm × 1 cm in size with tweezers, and spread on the induction medium with the adaxial side facing upwards. The formula is to add 0.1mg / L NAA and 1.0mg / L 6-benzy...

Embodiment 2

[0039] Embodiment 2 Hemerocallis grandiflora blade tissue culture

[0040] In this embodiment, a wild leaf of Hemerocallis fulva is selected for tissue culture.

[0041] 1) Selection and disinfection of explants: select a single plant of Hemerocallis grandiflora with a large number of new leaves, select new leaves with a leaf age of 9-10 days, and intercept the young leaves above the stem tip to ensure as much as possible Consistency of explants. Rinse the explants with running water for 3-5 minutes, then sterilize the surface with 70% alcohol for 30 seconds, rinse with sterile water three times, then soak in 2% NaClO for 10-15 minutes, and then rinse with sterile water for 5-6 times.

[0042] 2) Induction of callus: In the ultra-clean workbench, the leaves treated in step 1) were torn into long squares of 1 cm × 1 cm in size with tweezers, and spread on the induction medium with the adaxial side facing upwards. The formula is to add 0.2mg / L NAA and 1.0mg / L 6-benzylaminopurine...

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Abstract

The invention relates to a method for tissue culture of Hemerocallis grandiflora leaves. The specific steps are: taking the young inner leaves of Hemerocallis grandiflora grown in the open field as explants, inoculating them in induction medium to induce callus formation after disinfection treatment Tissues, through callus differentiation and proliferation, bud induction and rooting induction, the complete seedlings of Hemerocallis grandiflora are formed, and the seedlings of Hemerocallis grandiflora are transplanted into the matrix to grow into normal Hemerocallis grandiflora plants. The invention uses the leaves of the daylilies for tissue culture, overcomes the restriction that the daylilies can only use flower parts for tissue culture and multiplication in the flowering stage, can realize annual reproduction, is beneficial to rapid multiplication of improved varieties, and enriches the types of daylilies used in gardens.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for tissue culture and multiplication of young leaves of Hemerocallis grandiflorum growing in the open field. Background technique [0002] Hemerocallis grandiflora is a perennial herb of the Hemerocallis family Hemerocallis genus, and it is an important perennial flower. The germplasm resources of Hemerocallis grandis are very rich in China. There are 14 species of this genus found in the world, and there are 11 species of Hemerocallis grandiflora in China. On the basis of wild Hemerocallis germplasm, there are more than 80,000 species of Hemerocallis grandiflora horticultural cultivars that have been artificially cross-bred. The leaves of Hemerocallis grandiflora are emerald green, narrow and long, basal, arranged in two rows in a belt shape, with spindle-shaped fleshy roots and fibrous roots. The stem is short and root-like, the flower moss is pulled o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 高亦珂朱琳王叶任毅张启翔王佳程堂仁
Owner BEIJING FORESTRY UNIVERSITY
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