High-efficiency method for induction and successive transfer proliferation regeneration of banana embryogenic calluses

Abstract

The invention discloses a high-efficiency method for the induction and the successive transfer proliferation regeneration of banana embryogenic calluses. The technological process comprises a culture process of inducing dedifferentiated calluses, a culture process of inducing fragile embryogenic calluses, a successive transfer proliferation process of the fragile embryogenic calluses, an inducing and embryo-forming process of the embryogenic calluses and an inducing and seedling process of mature embryos. The invention has the advantages of simple technological process, low production cost, high embryogenic tissue induction and proliferation rate and long successive transfer time, solves the problems of low embryonic reaction of an exophyte, hard induction of the embryogenic calluses, low induction embryo-forming and seedling rates, long induction period, and the like in the culture process of banana tissues, provides a new technological route for a banana transform technology and provides a high-efficiency regeneration system for producing juvenal tissue and culturing banana seedlings, thereby having a great application value.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for efficiently inducing banana embryogenic callus, achieving long-term subculture and proliferation through plant tissue culture technology, and inducing the subcultured embryogenic callus to form embryos and seedlings Methods. Background technique [0002] Banana is an important economic crop with both fruit and food in tropical and subtropical developing countries. As the world's fourth largest food crop after rice, wheat, and corn, the healthy development of the banana industry has an important socio-economic status. In 1985, the South China Institute of Botany, Chinese Academy of Sciences took the lead in successfully industrializing the production technology of banana tissue culture seedlings. At present, banana tissue culture technology is very mature and has been widely used in the production of banana seedlings. It has achieved remarkable economic and social be...

AI Technical Summary

Problems solved by technology

At present, the low induction rate of embryogenic callus, long induction time, and low embryonic and seedling formation rates are the main bottlenecks affecting the wide application of this receptor system.