26 results about "Cell function" patented technology

The application relates to a cell infiltration inference method and system fusing GO function annotation and PPI network information, and the method comprises the following steps: collecting gene expression data, GO function annotation data and PPI network data; constructing a cell-cell function correlation network and a cell-cell physical interaction network respectively; performing weighted fusion processing on the two networks to obtain a comprehensive cell relationship network; calculating a final cell infiltration score through a restart walk algorithm, and inferring the infiltration degree in a tumor microenvironment according to the final cell infiltration score. The application innovatively fuses GO function annotation information and PPI network data, comprehensively considers the functional similarity and physical or signal interaction between cells, enables the model to understand cell synergy from the biological pathway level and analyze cell direct interaction from the protein interaction level, avoids one-sidedness of a single perspective, and provides a more stereoscopic cognitive framework for tumor microenvironment analysis.

This invention discloses a method for constructing a type 2 diabetes mouse model combining artificial intelligence prediction and multi-target knockout. The method involves acquiring and standardizing multi-omics data and baseline phenotypic data of mice to form a standardized modeling dataset; constructing an interaction map covering pancreatic β-cell function based on this data; selecting target target combinations and target phenotypic prediction results; generating construction instructions and submitting them to a controlled facility for multi-gene knockout modification to obtain modified mice and modification receipts; obtaining measured phenotypic data of the modified mice based on the modification receipts; comparing this data with the target phenotypic prediction results to generate phenotypic deviation metrics and phenotypic determination results; and registering the target target combinations, prediction results, measured phenotypic data, and modification receipts when the phenotypic determination results are satisfactory to form a type 2 diabetes composite mouse model. This invention achieves closed-loop optimization of AI-driven multi-target genetic modification, improving the efficiency and accuracy of constructing animal models of complex metabolic diseases.

The application belongs to the technical field of molecular biology, and particularly relates to an anti-CD28 nanobody and a preparation method and application thereof. The anti-CD28 nanobody provided by the application is prepared in the following manner: constructing a CD28 antigen to immunize a llama, constructing a phage library to the PBMC of the immunized llama, screening the phage library, selecting correct sequences to construct and express and purify, and finally obtaining the anti-CD28 nanobody through cell function detection screening. The nanobody provided by the application has specific recognition and binding capacity for CD28, has the advantages of high specificity, high sensitivity and the like, effectively reduces the development and production cost of the CD28 antibody, and shortens the expression time of the antibody.

The application relates to the technical field of nanomaterials, and particularly discloses a kind of nanomaterials for stem cell labeling and photoacoustic imaging, a preparation method and application thereof.The application provides a new method capable of stably labeling high-performance photoacoustic nanomaterials on the surface of stem cells in a quick, efficient, specific and non-damaging manner to cell functions, so as to realize high signal-to-noise ratio and long-term dynamic in-vivo tracing of stem cell transplantation fate, and overcome multiple limitations of traditional endocytosis labeling in efficiency, timeliness, specificity and cell compatibility.

This invention discloses the application of SDC1 in the diagnosis and treatment of breast cancer. Through bioinformatics analysis, cell function experiments, multi-omics analysis, and animal model validation, this invention reveals for the first time the high expression of SDC1 in breast cancer and its correlation with poor patient prognosis. It elucidates the molecular mechanism by which SDC1 promotes the malignant progression of breast cancer by regulating ECM remodeling, EMT, angiogenesis, and ferroptosis resistance, and verifies the therapeutic potential of MZ1 in inhibiting breast cancer growth and metastasis by downregulating SDC1 expression. This invention provides the application of reagents for detecting SDC1 expression levels in the preparation of diagnostic or prognostic kits for breast cancer, as well as the application of SDC1 inhibitors in the preparation of drugs for treating breast cancer. This invention provides new technical solutions for the diagnosis, prognostic assessment, and treatment of breast cancer.

The invention provides a dynamic cell detection method based on super-resolution imaging and semi-quantification, and relates to the field of biomedical detection. The method comprises the following steps: constructing cell experiment groups and performing intervention; after dyeing by adopting a specific fluorescent probe, carrying out super-resolution imaging and image analysis by utilizing a structured light illumination microscope to obtain first semi-quantitative data; detecting by using a fluorescence microplate reader in parallel to obtain second data, and carrying out cross validation; and finally evaluating the drug function based on the valid data. According to the invention, the combination of subcellular morphology observation and multi-channel fluorescence semi-quantitative analysis is realized, and the accuracy and reliability of diabetes drug screening and cell function evaluation are remarkably improved.

The application provides a solid oxide fuel cell and a preparation method thereof, the solid oxide fuel cell comprises a metal support layer, a transition layer and a cell function layer which are sequentially stacked, the material of the metal support layer comprises a high-temperature alloy, and the thermal expansion coefficient of the transition layer is between the thermal expansion coefficient of the cell function layer and the thermal expansion coefficient of the metal support layer. Therefore, the solid oxide fuel cell of the application can solve the problem that the metal support solid oxide fuel cell cannot normally operate in a high-temperature environment of 1200 DEG C-1300 DEG C.

The present invention discloses a fluorescence in situ hybridization method for co-localization of multiple genes in shellfish hemocytes, belonging to the technical field of cell biology of marine invertebrates. By optimizing the osmotic pressure buffer system and the preparation method, the present invention can stably obtain a monolayer sample with complete and uniform cell morphology. By advancing the cell morphology imaging step, the complete morphological information of cells is obtained and fixed as a reference before implementing the stringent in situ hybridization process. The present invention can achieve in situ visualization of multiple target genes while better preserving the cell morphological structure, which helps to obtain more accurate and stable spatial distribution information of gene expression at the single-cell level, improves the overall reliability of the experiment, and provides a more effective analysis tool for the study of cell functions of marine invertebrates.

The application discloses application of cathepsin E in preparation of a drug for treating or preventing lung cancer, relates to the technical field of biological medicine, and first proves that CTSE is highly expressed in lung adenocarcinoma tissues and has the biological function of inhibiting lung cancer cell proliferation through clinical sample analysis, cell function experiment and animal experiment. Mechanism research shows that CTSE plays the anticancer role by down-regulating the expression of cathepsin B (CTSB) and promoting the degradation of STING protein through a lysosome pathway and inhibiting the cGAS-STING natural immune pathway. The application clarifies the application value of CTSE as a new target for treating lung adenocarcinoma, provides a theoretical basis and experimental basis for developing a novel lung cancer treatment drug, and has an important clinical application prospect.

The invention relates to the technical field of biology, and relates to a specific binding protein of NGF, a nucleic acid molecule, a carrier, a cell, a composition, a preparation method and application. The specific binding protein has an NGF binding structural domain a or / and an NGF binding structural domain b, wherein the NGF binding structural domain a has amino acid sequences such as HCDR1 to HCDR3 as shown in SEQ ID NO.19 to 21 and LCDR1 to LCDR3 as shown in SEQ ID NO.25 to 27; the NGF binding domain b has HCDR1 to HCDR3 of which the amino acid sequences are as shown in SEQ ID NO.22 to 24, and LCDR1 to LCDR3 of which the amino acid sequences are as shown in SEQ ID NO.28 to 30. The NGF / TrkA signal pathway-related pain inhibitor is specifically combined with NGF, has higher cell function activity and can be used for treating NGF / TrkA signal pathway-related pain.

This invention belongs to the field of molecular biology technology, specifically relating to an anti-CD28 nanobody, its preparation method, and its application. The preparation process of the anti-CD28 nanobody provided by this invention is as follows: CD28 antigen is constructed to immunize alpacas; a phage library is constructed from the immunized alpaca PBMCs; the phage library is screened, and correct sequences are selected for construction, expression, and purification; the anti-CD28 nanobody is finally obtained after screening by cell function detection. The nanobody provided by this invention has specific recognition and binding ability for CD28, exhibiting advantages such as high specificity and high sensitivity; simultaneously, it effectively reduces the development and production costs of CD28 antibodies and shortens antibody expression time.

The application belongs to the field of biological medicine, and particularly relates to a functionalized magnetic microsphere of Raphani Semen components, a preparation method and application thereof. Based on photo-crosslinking technology, the application constructs multi-component modified magnetic microspheres, solves the problems existing in traditional single-component probes, and simultaneously fixes multiple active components in a traditional Chinese medicine complex system on the surface of the magnetic microspheres. On this basis, the application integrates high-throughput identification technology of chemical proteomics and cell function verification experiments, systematically captures and identifies a target group acted on by a complex component system of traditional Chinese medicine, constructs an overall regulation network of components, targets and pathways, and analyzes the action mechanism of traditional Chinese medicine from the system biology level.

The present application belongs to the technical field of nucleic acid delivery and gene editing, and particularly relates to a method for knocking out a target gene of a cell in vitro by using an LNP delivery system. In view of the technical problems of unstable editing efficiency, large difference in editing efficiency between different individuals, low transfection efficiency, large cell damage, unstable knockout effect and the like when a T cell is transfected by using an existing LNP for gene editing, the present application provides a method for efficiently knocking out a target gene of a T cell in the form of LNP by optimizing the LNP and LNP transfection operation parameters in a systematic manner. The method can effectively solve the above technical bottlenecks, realize efficient and stable knockout of a target gene of a T cell, and provide reliable technical support for T cell function research and immunotherapy application.

The invention provides a bimodal microscopic imaging system and method, and relates to the technical field of microscopic imaging, and the system comprises a first imaging module which is configured to obtain a three-dimensional refractive index distribution image of a sample based on an optical diffraction tomography principle; the second imaging module is configured to acquire a fluorescence image of the sample based on a confocal microscopic imaging principle; the control module comprises a time sequence control unit and an image fusion unit; the time sequence control unit is configured to control the data acquisition time sequence of the first imaging module and the second imaging module; the image fusion unit is configured to process the three-dimensional refractive index distribution image and the fluorescence image to generate a fused image. According to the invention, unmarked three-dimensional form imaging and specific fluorescence imaging of living cells are realized, distribution of organelles in the three-dimensional form of the cells can be accurately positioned through bimodal data fusion, and linkage analysis of cell functions and forms is carried out.

The invention provides a method for marking a microfilament skeleton in a living cell by using ligand-modified magnetic nanoparticles and application of the microfilament skeleton. The method comprises the following steps: preparing ligand modified magnetic nanoparticles; culturing living cells to be marked to obtain adherent cells; incubating the adherent cells and the ligand modified magnetic nanoparticles to obtain marked cells; and observing the marked intracellular microfilament skeleton by using a laser confocal imaging system. The ligand-modified magnetic nanoparticles prepared by the method have good biocompatibility, magnetic responsiveness and microfilament targeted binding characteristics, microfilament skeletons in living cells are labeled by the ligand-modified magnetic nanoparticles, and then distribution of the microfilament skeletons in the living cells can be clearly observed by using a laser confocal imaging system. According to the method, the microfilament skeleton in the living cell can be marked and clearly imaged, the distribution of the microfilament skeleton in the living cell can be deeply observed, and the method has a good application prospect in the fields of biomedical treatment, cell function research and the like.

The invention discloses a method for constructing a porcine epididymis epithelial cell line stably proliferating in vitro by using T-tag lentivirus, and belongs to the technical field of biomedicine. By optimizing culture medium components suitable for lentivirus transduction and cell screening and amplification after transduction, the growth state of the constructed porcine epididymis epithelial cell line is remarkably improved, the clone forming ability of the porcine epididymis epithelial cell line is enhanced, and the cell apoptosis rate is effectively reduced. The culture medium system provides key support for stably and efficiently establishing a porcine epididymis epithelial cell line by utilizing a lentivirus technology, so that a solid foundation is laid for researching downstream molecular mechanisms, cell functions, related disease models and the like.

To provide a system and method for predicting insulin resistance and / or a pancreatic β-cell function.SOLUTION: A system disclosed herein uses a machine-learning model to predict insulin resistance and / or a decline in a pancreatic β-cell function of a subject in need thereof based on a feature set extracted from a database. As such, clinicians or the subject can be warned to take necessary actions and adjust a related medical treatment or lifestyle before the subject is diagnosed with diabetes mellitus. A computer-readable medium therefor is also provided.SELECTED DRAWING: Figure 1

The application belongs to the field of biological medicine, and discloses a novel targeted delivery system for treating severe ulcerative colitis and application, which comprises the following steps: S101, intracellular gelation process: the Akk bacteria are amplified to the logarithmic growth phase, and after centrifugal resuspension, the bacteria are incubated with BPQDs-NH2 and gel buffer; the components are promoted into cells through a single freeze-thaw cycle; S102, cross-linking reaction: a cross-linking reaction is initiated by using 365nm, 1600mJ / cm² ultraviolet light, and a targeted delivery system GAkk@BP system is obtained through purification. The GAkk@BP oral delivery system constructed in the application has high ROS scavenging capacity, can significantly reduce the intestinal epithelial cell damage induced by ROS, and effectively protect the cell function; in addition, the intracellular gelation modification has realized the precise regulation of Akk proliferation, successfully avoided the risk of excessive colonization, and laid a solid foundation for the subsequent optimization of the intracellular gelation probiotic delivery system, the in-depth analysis of the proliferation blocking / metabolic isolation and the mechanism of the synergistic treatment of UC.

The invention discloses a tunnel nanotube mediated intercellular mechanical signal transduction regulation and control mechanism research method and system and application, and relates to the technical field of biology. The regulation and control mechanism research method of tunnel nanotube mediated intercellular mechanical signal transduction comprises the following steps: S1, providing tunnel nanotube connection cells growing on a polyacrylamide hydrogel substrate; s2, performing drug treatment on the tunnel nanotube connected cells: applying an effective dose of at least one signal path inhibitor into a system in which the tunnel nanotube connected cells are cultured; s3, mechanical stimulation of tunnel nanotube connection cells; s4, measuring the change of the traction force of the cells and the change of the spreading area of the cells; by adopting the research method provided by the invention, the important influence of intercellular electric signal conduction on the cell connection function of the tunnel nanotube is revealed, and a precise tool is provided for researching intercellular mechanical communication.

The invention belongs to the technical field of breast cancer treatment biological medicines, and particularly relates to application of a target protein DLGAP1 inhibitor in preparation of medicines for preventing, treating or delaying breast cancer development. Through systematic cell function experiments and nude mouse tumorigenesis experiments, it is found that proliferation, migration and invasion ability of breast cancer cells can be remarkably inhibited by using the inhibitor to specifically inhibit expression of the protein DLGAP1, tumor growth is effectively inhibited, and the effectiveness of the inhibitor of the protein DLGAP1 in the aspect of inhibiting breast cancer growth is proved. The invention not only deepens the understanding of breast cancer pathogenesis in theory, but also provides a solid foundation for developing novel breast cancer targeted therapeutic drugs in practice.

A device for treating eye disorders, particularly disorders of the internal tissues of the eye, comprises a mask and a plurality of light-emitting diodes (LEDs) arranged on the inner surface of the mask. The plurality of LEDs includes a matrix of at least one LED arranged in a region of the inner surface of the mask that is adapted to face the user's eyeball area when in use, and is configured to emit electromagnetic radiation adapted to stimulate the cellular function of retinal cells.

The invention belongs to the technical field of stem cell directional differentiation, and particularly relates to a method for efficiently inducing stem cells to be directionally differentiated into specific functional cells by combining a histone deacetylase inhibitor and a small molecule inducer. The technology aims at solving the problems that the existing stem cell directional differentiation efficiency is low, the differentiated cell function is incomplete and the like, the stem cell differentiation process is accurately regulated and controlled through an innovative combined induction strategy, and a key technical support is provided for wide application of stem cells in the fields of regenerative medicine, drug research and development, disease model construction and the like. In regenerative medicine, the cells expected to be differentiated can be used for repairing damaged tissues and organs; in the aspect of drug research and development, differentiated cells can be used for drug screening and drug effect evaluation; in the aspect of disease model construction, the disease occurrence and development process can be simulated, and disease mechanism research is assisted.

The application provides a weak signal and background noise automatic identification method and system based on deep learning, and relates to the technical field of flow cytometry. The method comprises the following steps: analyzing historical flow detection data, extracting historical single cell events and multi-dimensional fluorescence signal features; screening target feature dimensions based on cell functional indicators, labeling real signal events and background noise events to construct a training data set; constructing a deep learning model comprising a feature extraction network and a discrimination network; training the deep learning model using the training data set, so that it fully learns the small distribution boundary differences in the multi-dimensional space, and obtains a trained automatic identification model; analyzing to-be-detected flow detection data to extract to-be-detected single cell events, inputting the to-be-detected single cell events into the automatic identification model for event-by-event discrimination, and identifying functional positive cell groups based on the output classification results. The application effectively overcomes the subjectivity of manual gate setting.

The application discloses a high-purity NKT cell preparation and a preparation method and a cryopreservation regulation method, and belongs to the fields of cell biology technology and immunotherapy. The method systematically solves multiple challenges of low purity, insufficient amplification efficiency, poor cell viability, high cost and difficulty in large-scale production of NKT cells in the prior art through a unique secondary cryopreservation process, innovative application of platelet lysate and specific induction of alpha-galactosylceramide (alpha-GalCer). The prepared NKT cell preparation has the advantages of high purity, large amplification fold, strong cell function activity and good safety, and provides a high-quality and standardized cell source for clinical applications such as tumor immunotherapy, and has great industrialization prospects.

The invention belongs to the technical field of biology, and particularly relates to application of Pt5-1c in preparation of a medicine for promoting angiogenesis and accelerating wound healing. It is found for the first time that Pt5-1c can effectively promote angiogenesis in vitro and in vivo. The invention relates to a novel compound which enhances endothelial cell functions, accelerates mouse vascular repair and rescues vascular defects of zebrafish, these effects are mediated by a pleiotropic mechanism, including up-regulation of pro-angiogenic factors and activation of PI3K / AKT / mTOR and p38 MAPK signaling pathways, and in combination with established safety characteristics and validated efficacy in tissue repair, the compound can be used for preparing a novel compound, and the compound can be used for preparing a novel compound. Pt5-1c represents a regenerative therapeutic agent with a prospect.

The embodiment of the invention discloses a cell functionalization stage classification method, a cell functionalization stage determination method, a cell functionalization stage classification device, a cell functionalization stage determination device and electronic equipment. The classification method comprises the steps that a quasi-time sequence value of cells in a target cell cluster is acquired, the quasi-time sequence value is determined based on quasi-time sequence analysis, and the target cell cluster is a cell cluster obtained after cell clustering; determining continuous density distribution of the cells in the target cell cluster according to the quasi-time sequence value of the cells in the target cell cluster; according to the continuous density distribution and a fitting model corresponding to the shape of the continuous density distribution, fitting the continuous density distribution to obtain a fitting function; and determining a cell functionalization stage contained in the target cell cluster and a quasi-time sequence value range corresponding to the cell functionalization stage based on the fitting function. By adopting the embodiment of the invention, the cell functionalization stage can be determined without depending on a cell annotation result.