40 results about "Cell function" patented technology

The application relates to a cell infiltration inference method and system fusing GO function annotation and PPI network information, and the method comprises the following steps: collecting gene expression data, GO function annotation data and PPI network data; constructing a cell-cell function correlation network and a cell-cell physical interaction network respectively; performing weighted fusion processing on the two networks to obtain a comprehensive cell relationship network; calculating a final cell infiltration score through a restart walk algorithm, and inferring the infiltration degree in a tumor microenvironment according to the final cell infiltration score. The application innovatively fuses GO function annotation information and PPI network data, comprehensively considers the functional similarity and physical or signal interaction between cells, enables the model to understand cell synergy from the biological pathway level and analyze cell direct interaction from the protein interaction level, avoids one-sidedness of a single perspective, and provides a more stereoscopic cognitive framework for tumor microenvironment analysis.

The invention relates to a cell infiltration inference method and system fusing GO function annotation and PPI network information. The method comprises the following steps: collecting gene expression data, GO function annotation data and PPI network data; respectively constructing a cell * cell function association network and a cell * cell physical interaction network; carrying out weighted fusion processing on the two to obtain a comprehensive cell relation network; and calculating a final cell infiltration fraction through a restart migration algorithm, and deducing the infiltration degree in the tumor microenvironment according to the final cell infiltration fraction. According to the method, GO function annotation information and PPI network data are creatively fused, functional similarity and physical or signal interaction between cells are comprehensively considered, the model can understand cell synergy from the biological pathway level and can analyze cell direct interaction from the protein interaction level, one-sidedness of a single view angle is avoided, and the method has the advantages of being simple in structure and convenient to operate. And a more three-dimensional cognitive framework is provided for tumor microenvironment analysis.

This invention discloses a method for constructing a type 2 diabetes mouse model combining artificial intelligence prediction and multi-target knockout. The method involves acquiring and standardizing multi-omics data and baseline phenotypic data of mice to form a standardized modeling dataset; constructing an interaction map covering pancreatic β-cell function based on this data; selecting target target combinations and target phenotypic prediction results; generating construction instructions and submitting them to a controlled facility for multi-gene knockout modification to obtain modified mice and modification receipts; obtaining measured phenotypic data of the modified mice based on the modification receipts; comparing this data with the target phenotypic prediction results to generate phenotypic deviation metrics and phenotypic determination results; and registering the target target combinations, prediction results, measured phenotypic data, and modification receipts when the phenotypic determination results are satisfactory to form a type 2 diabetes composite mouse model. This invention achieves closed-loop optimization of AI-driven multi-target genetic modification, improving the efficiency and accuracy of constructing animal models of complex metabolic diseases.

The application belongs to the technical field of molecular biology, and particularly relates to an anti-CD28 nanobody and a preparation method and application thereof. The anti-CD28 nanobody provided by the application is prepared in the following manner: constructing a CD28 antigen to immunize a llama, constructing a phage library to the PBMC of the immunized llama, screening the phage library, selecting correct sequences to construct and express and purify, and finally obtaining the anti-CD28 nanobody through cell function detection screening. The nanobody provided by the application has specific recognition and binding capacity for CD28, has the advantages of high specificity, high sensitivity and the like, effectively reduces the development and production cost of the CD28 antibody, and shortens the expression time of the antibody.

The present invention relates to the field of biomedicine and particularly provides a method for identifying a target. The method specifically comprises determining the number of target-adjusting systems of a plurality of cells to be tested to determine the influence of adjusting a candidate target on cell function; and before and / or during the determination, adjusting the expression or activity of the candidate target in said plurality of cells by means of the plurality of target-adjusting systems, wherein each of the target-adjusting systems targets a different candidate target site, and during the determination, the number of said cells is at least 5 times the number of the target-adjusting systems. Further provided is a system for identifying a target.

The application relates to the technical field of nanomaterials, and particularly discloses a kind of nanomaterials for stem cell labeling and photoacoustic imaging, a preparation method and application thereof.The application provides a new method capable of stably labeling high-performance photoacoustic nanomaterials on the surface of stem cells in a quick, efficient, specific and non-damaging manner to cell functions, so as to realize high signal-to-noise ratio and long-term dynamic in-vivo tracing of stem cell transplantation fate, and overcome multiple limitations of traditional endocytosis labeling in efficiency, timeliness, specificity and cell compatibility.

The invention belongs to the technical field of nano medicine, and particularly discloses a method for obtaining exosomes with different cell biological functions, which comprises the following steps: preparing hybrid cells HCs integrating different cell functions by a cell fusion method, and further collecting exosomes H / Exos derived from the hybrid cells. The invention also discloses the exosome and application of the exosome in preparation of drugs. Different hybrid cells have multiple functions of cells before fusion, and exosomes secreted by the hybrid cells inherit the functions of the hybrid cells. The hybrid cells can continuously secrete exosomes with the characteristics of the two parent cells in the proliferation process. The defect that the end-to-end differentiated cells cannot be proliferated is overcome, so that large-scale preparation of the natural multifunctional exosome can be realized, and a new method is provided for customized exosome preparation for disease treatment.

This invention discloses the application of SDC1 in the diagnosis and treatment of breast cancer. Through bioinformatics analysis, cell function experiments, multi-omics analysis, and animal model validation, this invention reveals for the first time the high expression of SDC1 in breast cancer and its correlation with poor patient prognosis. It elucidates the molecular mechanism by which SDC1 promotes the malignant progression of breast cancer by regulating ECM remodeling, EMT, angiogenesis, and ferroptosis resistance, and verifies the therapeutic potential of MZ1 in inhibiting breast cancer growth and metastasis by downregulating SDC1 expression. This invention provides the application of reagents for detecting SDC1 expression levels in the preparation of diagnostic or prognostic kits for breast cancer, as well as the application of SDC1 inhibitors in the preparation of drugs for treating breast cancer. This invention provides new technical solutions for the diagnosis, prognostic assessment, and treatment of breast cancer.

The invention provides a dynamic cell detection method based on super-resolution imaging and semi-quantification, and relates to the field of biomedical detection. The method comprises the following steps: constructing cell experiment groups and performing intervention; after dyeing by adopting a specific fluorescent probe, carrying out super-resolution imaging and image analysis by utilizing a structured light illumination microscope to obtain first semi-quantitative data; detecting by using a fluorescence microplate reader in parallel to obtain second data, and carrying out cross validation; and finally evaluating the drug function based on the valid data. According to the invention, the combination of subcellular morphology observation and multi-channel fluorescence semi-quantitative analysis is realized, and the accuracy and reliability of diabetes drug screening and cell function evaluation are remarkably improved.

The application provides a solid oxide fuel cell and a preparation method thereof, the solid oxide fuel cell comprises a metal support layer, a transition layer and a cell function layer which are sequentially stacked, the material of the metal support layer comprises a high-temperature alloy, and the thermal expansion coefficient of the transition layer is between the thermal expansion coefficient of the cell function layer and the thermal expansion coefficient of the metal support layer. Therefore, the solid oxide fuel cell of the application can solve the problem that the metal support solid oxide fuel cell cannot normally operate in a high-temperature environment of 1200 DEG C-1300 DEG C.

The present invention discloses a fluorescence in situ hybridization method for co-localization of multiple genes in shellfish hemocytes, belonging to the technical field of cell biology of marine invertebrates. By optimizing the osmotic pressure buffer system and the preparation method, the present invention can stably obtain a monolayer sample with complete and uniform cell morphology. By advancing the cell morphology imaging step, the complete morphological information of cells is obtained and fixed as a reference before implementing the stringent in situ hybridization process. The present invention can achieve in situ visualization of multiple target genes while better preserving the cell morphological structure, which helps to obtain more accurate and stable spatial distribution information of gene expression at the single-cell level, improves the overall reliability of the experiment, and provides a more effective analysis tool for the study of cell functions of marine invertebrates.

The application discloses application of cathepsin E in preparation of a drug for treating or preventing lung cancer, relates to the technical field of biological medicine, and first proves that CTSE is highly expressed in lung adenocarcinoma tissues and has the biological function of inhibiting lung cancer cell proliferation through clinical sample analysis, cell function experiment and animal experiment. Mechanism research shows that CTSE plays the anticancer role by down-regulating the expression of cathepsin B (CTSB) and promoting the degradation of STING protein through a lysosome pathway and inhibiting the cGAS-STING natural immune pathway. The application clarifies the application value of CTSE as a new target for treating lung adenocarcinoma, provides a theoretical basis and experimental basis for developing a novel lung cancer treatment drug, and has an important clinical application prospect.

The present application relates to the technical field of database construction, and particularly relates to a method and system for constructing a reproductive medicine database. The method comprises the following steps: obtaining a patient biological tissue sample; obtaining reproductive endocrine hormone data, embryo morphology parameter data, immune cell function data and genomic variation data of the patient biological tissue sample; performing time series analysis and processing on hormone fluctuation patterns according to the reproductive endocrine hormone data to obtain endocrine dynamic characteristic data; performing image recognition processing based on light coherence superposition characteristics on the embryo morphology parameter data, and performing embryo development potential evaluation to obtain embryo development potential data; and performing hierarchical clustering processing on patient individual heterogeneity according to the immune cell function data and the genomic variation data to obtain patient immune-heredity typing data. The present application realizes standardization of data acquisition through quantum dot sensing technology and a multi-channel microfluidic chip, and solves the problem of heterogeneity of data sources.

The invention provides a cell function deployment method and device, a medium and a program product. The method comprises the steps that first feature data of a target cell and a function strategy model corresponding to a scene of the target cell are acquired; inputting the first feature data into the function strategy model to obtain performance prediction values corresponding to multiple function strategies; and determining a target function strategy deployed to the target cell from the multiple function strategies according to the performance prediction value. According to the method provided by the embodiment of the invention, the target function strategy matched with the scene where the cell is located can be determined, so that the comprehensive performance of different cells can be optimal in different scenes, the throughput of the cell is improved, and the user experience is improved.

The invention provides an endometrial stem cell preparation as well as a preparation method and application thereof, and belongs to the technical field of biological preparations. According to the preparation method of the endometrial stem cell preparation, the safety risk is controlled through an autoserum source, the cell function is optimized in combination with a low-oxygen environment, and a high-stability and low-immune-risk standardized solution is provided for clinical transformation of the endometrial stem cell preparation. Three batches of culture data show that the cell viability is gt; the mesenchymal stem cell culture medium is stable in form and meets the mesenchymal stem cell verification standard. The flow detection verification shows that the positive rate of CD73 / CD90 / CD105 is greater than or equal to 95%, the negative markers such as CD34 / CD45 and the like are less than or equal to 2%, and the high purity is proved; the cell cycle shows that the G0 / G1 period accounts for 74-80%, and the resting state and the genome stability are maintained. After the preparation provided by the invention is stored for a short time or frozen and unfrozen for a long time, the cell morphology and proliferation capacity are not obviously attenuated.

The invention relates to the technical field of biology, and relates to a specific binding protein of NGF, a nucleic acid molecule, a carrier, a cell, a composition, a preparation method and application. The specific binding protein has an NGF binding structural domain a or / and an NGF binding structural domain b, wherein the NGF binding structural domain a has amino acid sequences such as HCDR1 to HCDR3 as shown in SEQ ID NO.19 to 21 and LCDR1 to LCDR3 as shown in SEQ ID NO.25 to 27; the NGF binding domain b has HCDR1 to HCDR3 of which the amino acid sequences are as shown in SEQ ID NO.22 to 24, and LCDR1 to LCDR3 of which the amino acid sequences are as shown in SEQ ID NO.28 to 30. The NGF / TrkA signal pathway-related pain inhibitor is specifically combined with NGF, has higher cell function activity and can be used for treating NGF / TrkA signal pathway-related pain.

This invention belongs to the field of molecular biology technology, specifically relating to an anti-CD28 nanobody, its preparation method, and its application. The preparation process of the anti-CD28 nanobody provided by this invention is as follows: CD28 antigen is constructed to immunize alpacas; a phage library is constructed from the immunized alpaca PBMCs; the phage library is screened, and correct sequences are selected for construction, expression, and purification; the anti-CD28 nanobody is finally obtained after screening by cell function detection. The nanobody provided by this invention has specific recognition and binding ability for CD28, exhibiting advantages such as high specificity and high sensitivity; simultaneously, it effectively reduces the development and production costs of CD28 antibodies and shortens antibody expression time.

The present application provides systems and methods for measuring cell function or tissue function. More particularly, the present application provides systems and methods for determining cell or tissue contractility using ultrasound; systems and methods for measuring the effect an agent has on cell or tissue contractility using ultrasound; and systems and methods for determining material properties of a material using ultrasound.

The invention provides a < 68 > Ga-labeled small molecule compound as well as a preparation method and application thereof, and belongs to the technical field of radiopharmaceuticals and nuclear medicine. The [68Ga] Ga-DTPA-EOB probe marked by 68Ga is suitable for positron emission tomography, has better temporal-spatial resolution and dynamic imaging capability, and can quantify tracer distribution. Under the condition that the pH value of the < 68 > Ga is 3.8-4.0, < 68 > Ga labeling can be completed only within 5-10 minutes at room temperature, the labeling efficiency is high (the labeling rate is greater than or equal to 95%), and the amount of EOB-DTPA required for labeling is extremely small. The radioactive activity needed by PET imaging is low, the radiation burden on a patient is small, and the gadolinium deposition risk is avoided. The [68Ga] Ga-DTPA-EOB probe is particularly suited for scenes requiring rapid diagnosis, avoiding MRI contraindications, or requiring multi-modal imaging.

The application belongs to the field of biological medicine, and particularly relates to a functionalized magnetic microsphere of Raphani Semen components, a preparation method and application thereof. Based on photo-crosslinking technology, the application constructs multi-component modified magnetic microspheres, solves the problems existing in traditional single-component probes, and simultaneously fixes multiple active components in a traditional Chinese medicine complex system on the surface of the magnetic microspheres. On this basis, the application integrates high-throughput identification technology of chemical proteomics and cell function verification experiments, systematically captures and identifies a target group acted on by a complex component system of traditional Chinese medicine, constructs an overall regulation network of components, targets and pathways, and analyzes the action mechanism of traditional Chinese medicine from the system biology level.

The present application belongs to the technical field of nucleic acid delivery and gene editing, and particularly relates to a method for knocking out a target gene of a cell in vitro by using an LNP delivery system. In view of the technical problems of unstable editing efficiency, large difference in editing efficiency between different individuals, low transfection efficiency, large cell damage, unstable knockout effect and the like when a T cell is transfected by using an existing LNP for gene editing, the present application provides a method for efficiently knocking out a target gene of a T cell in the form of LNP by optimizing the LNP and LNP transfection operation parameters in a systematic manner. The method can effectively solve the above technical bottlenecks, realize efficient and stable knockout of a target gene of a T cell, and provide reliable technical support for T cell function research and immunotherapy application.

A system and a method for predicting insulin resistance and / or pancreatic β-cell function are provided, where a machine learning model is utilized to predict insulin resistance and / or pancreatic a decline of β-cell function of a subject in need thereof based on a feature set extracted from a database. Therefore, clinicians or the subject can be warned to take necessary actions on, and adjust related medical treatment or lifestyle before the subject is diagnosed with diabetes mellitus. In addition, a computer readable medium thereof is also provided.

The invention provides a bimodal microscopic imaging system and method, and relates to the technical field of microscopic imaging, and the system comprises a first imaging module which is configured to obtain a three-dimensional refractive index distribution image of a sample based on an optical diffraction tomography principle; the second imaging module is configured to acquire a fluorescence image of the sample based on a confocal microscopic imaging principle; the control module comprises a time sequence control unit and an image fusion unit; the time sequence control unit is configured to control the data acquisition time sequence of the first imaging module and the second imaging module; the image fusion unit is configured to process the three-dimensional refractive index distribution image and the fluorescence image to generate a fused image. According to the invention, unmarked three-dimensional form imaging and specific fluorescence imaging of living cells are realized, distribution of organelles in the three-dimensional form of the cells can be accurately positioned through bimodal data fusion, and linkage analysis of cell functions and forms is carried out.

The invention provides a method for marking a microfilament skeleton in a living cell by using ligand-modified magnetic nanoparticles and application of the microfilament skeleton. The method comprises the following steps: preparing ligand modified magnetic nanoparticles; culturing living cells to be marked to obtain adherent cells; incubating the adherent cells and the ligand modified magnetic nanoparticles to obtain marked cells; and observing the marked intracellular microfilament skeleton by using a laser confocal imaging system. The ligand-modified magnetic nanoparticles prepared by the method have good biocompatibility, magnetic responsiveness and microfilament targeted binding characteristics, microfilament skeletons in living cells are labeled by the ligand-modified magnetic nanoparticles, and then distribution of the microfilament skeletons in the living cells can be clearly observed by using a laser confocal imaging system. According to the method, the microfilament skeleton in the living cell can be marked and clearly imaged, the distribution of the microfilament skeleton in the living cell can be deeply observed, and the method has a good application prospect in the fields of biomedical treatment, cell function research and the like.

The invention discloses a method for constructing a porcine epididymis epithelial cell line stably proliferating in vitro by using T-tag lentivirus, and belongs to the technical field of biomedicine. By optimizing culture medium components suitable for lentivirus transduction and cell screening and amplification after transduction, the growth state of the constructed porcine epididymis epithelial cell line is remarkably improved, the clone forming ability of the porcine epididymis epithelial cell line is enhanced, and the cell apoptosis rate is effectively reduced. The culture medium system provides key support for stably and efficiently establishing a porcine epididymis epithelial cell line by utilizing a lentivirus technology, so that a solid foundation is laid for researching downstream molecular mechanisms, cell functions, related disease models and the like.

The invention relates to the technical field of biotechnology and cell engineering, in particular to a large-scale culture method of central memory T cells (TCM). According to the method, the lymphocyte expressed by the high homing receptor is activated by adopting an alternating electric field, and the cell activity is improved by combining with a composite metabolism regulator. A two-photon excitation three-dimensional scaffold of a modified collagen peptide, ferroferric oxide and a quantum dot labeled organoid is utilized to simulate an in-vivo microenvironment to promote cell anchoring. And finally, through gene editing, engineering a cryopreservation system of saccharomyces cerevisiae and ultraviolet light activated autophagic peptide, so as to realize the enhancement of cell function stability and long-term memory characteristic. According to the invention, the limitation of traditional two-dimensional culture is broken through, and an efficient and controllable T cell preparation scheme is provided for tumor immunotherapy, autoimmune disease regulation and personalized cell therapy through systematic integration of physical field regulation, bionic scaffold design and genetic engineering means.

The invention provides composite hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biology. The composite hydrogel comprises a temperature-sensitive physical cross-linked network skeleton which is formed by physically cross-linking at least one temperature-sensitive polymer and at least one polymer capable of forming a hydrogen bond or a crystal domain; the composite chemical crosslinking network is formed by carrying out esterification reaction and photochemical crosslinking reaction on at least one hydrophilic polyhydroxy polymer and at least one photo-crosslinking monomer containing unsaturated double bonds; and the biocompatibility enhancer is a hydrophilic polymer or a block copolymer. According to the hydrogel disclosed by the invention, the balance which cannot be considered by a traditional material is realized in the three aspects of mechanical property, pore connectivity and cell function adaptability, so that the problems of high sphere collapse rate, limited nutrient diffusion, low pelletizing rate and the like generally existing in the conventional hydrogel in the three-dimensional pelletizing culture of cells are systematically solved.

To provide a system and method for predicting insulin resistance and / or a pancreatic β-cell function.SOLUTION: A system disclosed herein uses a machine-learning model to predict insulin resistance and / or a decline in a pancreatic β-cell function of a subject in need thereof based on a feature set extracted from a database. As such, clinicians or the subject can be warned to take necessary actions and adjust a related medical treatment or lifestyle before the subject is diagnosed with diabetes mellitus. A computer-readable medium therefor is also provided.SELECTED DRAWING: Figure 1

The application belongs to the field of biological medicine, and discloses a novel targeted delivery system for treating severe ulcerative colitis and application, which comprises the following steps: S101, intracellular gelation process: the Akk bacteria are amplified to the logarithmic growth phase, and after centrifugal resuspension, the bacteria are incubated with BPQDs-NH2 and gel buffer; the components are promoted into cells through a single freeze-thaw cycle; S102, cross-linking reaction: a cross-linking reaction is initiated by using 365nm, 1600mJ / cm² ultraviolet light, and a targeted delivery system GAkk@BP system is obtained through purification. The GAkk@BP oral delivery system constructed in the application has high ROS scavenging capacity, can significantly reduce the intestinal epithelial cell damage induced by ROS, and effectively protect the cell function; in addition, the intracellular gelation modification has realized the precise regulation of Akk proliferation, successfully avoided the risk of excessive colonization, and laid a solid foundation for the subsequent optimization of the intracellular gelation probiotic delivery system, the in-depth analysis of the proliferation blocking / metabolic isolation and the mechanism of the synergistic treatment of UC.

The invention discloses a tunnel nanotube mediated intercellular mechanical signal transduction regulation and control mechanism research method and system and application, and relates to the technical field of biology. The regulation and control mechanism research method of tunnel nanotube mediated intercellular mechanical signal transduction comprises the following steps: S1, providing tunnel nanotube connection cells growing on a polyacrylamide hydrogel substrate; s2, performing drug treatment on the tunnel nanotube connected cells: applying an effective dose of at least one signal path inhibitor into a system in which the tunnel nanotube connected cells are cultured; s3, mechanical stimulation of tunnel nanotube connection cells; s4, measuring the change of the traction force of the cells and the change of the spreading area of the cells; by adopting the research method provided by the invention, the important influence of intercellular electric signal conduction on the cell connection function of the tunnel nanotube is revealed, and a precise tool is provided for researching intercellular mechanical communication.