74results about How to "Weak toxicity" patented technology

The invention discloses an ultrasonic coupling agent and application of the ultrasonic coupling agent in ultrasonic inspection or auxiliary treatment of clinical cavity canals, mucous membranes, non-intact skin and the like in hospital. The ultrasonic coupling agent consists of the following raw materials: safe and nontoxic silver ions or nanometer silver serving as a disinfectant, and bio-compatibility macromolecular materials serving as a viscosity regulator, an anti-discoloration agent, an anti-precipitant agent, and an anti-stimulant agent. The ultrasonic coupling agent meets the requirements on ultrasonic impedance and no corrosion, has the characteristics of disinfection, sterilization, safety, nontoxicity, no stimulation and the like, can effectively avoid cross infection in ultrasonic clinics, can be suitable for detecting the cavity canals, the mucous membranes and the non-intact skin, and has high medical clinical value. The ultrasonic coupling agent has a simple preparation method, and low cost of raw materials.

The present invention discloses a coronary artery medicine coating scaffold for inhibiting endometrial hyperplasia and preventing restenosis in the scaffold. It includes dilating scaffold base body and inner coating layer coated on the dilating scaffold base body, the above-mentioned inner coating layer is formed from medicine carrier and ligustrazine, their mass ratio is 3:1-1:3, the above-mentioned inner coating layer is one of methyl acrylate, methyl methacrylate copolymer, polylactic acid, polyglycollic acid, polymonoracemic lactic acid, polylactide-diglycolide polymer, phosphorylcholine, ethyl polyurethane inorganic micropore aluminium oxide and cellulose or their composite.

The invention discloses a method for removing bromine from disperse dye wastewater. The method comprises the following steps: firstly, adjusting the pH value of the disperse dye wastewater to be 2-5, adding a fenton reagent, and reacting at 40-60 DEG C for 0.5-2 hours; adding a flocculant to the wastewater, adjusting into alkalinity, standing and filtering to obtain filtrate I; adding an absorbent to the filtrate I, standing again and filtering to obtain filtrate II; and concentrating the filtrate II and recovering sulphate. According to the method, organic matters and bromine in the disperse dye wastewater can be effectively removed; the removal rate on high-concentration bromine and chromaticity can be up to over 99%; the removal rate of the organic matters can be up to over 75%; excellent sulphate is recovered; and the method is simple and easy to operate, and high in removal rate.

A rapid breeding method of Artemisia annua. Young stems on a single plant or a plurality of plants of Artemisia annua with completely consistent genotype are cut off; the young stems are disinfected in vitro and transversely cut into stem segments, and each stem segment has a bud; and the stem segments are cultured directly in a rooting medium and transplanted for soil culture after rooting. The method of the invention has easily available material, low variation rate, high increment coefficient, short rooting period, a two-week proliferation time reaching higher than 200 times, a rooting rate reaching higher than 90%, a transplanting survival rate reaching higher than 95% and strong factory production capability. Using the method of the invention to breed high-yield Artemisia annua linescan enable progeny to get stable heredity, so as to effectively reduce production cost of artemisinin.

The invention relates to the technical fields of veterinarian virus molecule biology and immunity, and discloses a recombinant gene VIII-type Newcastle disease virus attenuated strain NDV-rHR09-LaSota-HN. A preservation number of the attenuated strain NDV-rHR09-LaSota-HN is CCTCC (China Center For Type Culture Collection) NO: V201830. The research shows that the NDV-rHR09-LaSota-HN strain is weakin toxicity, the MDT is greater than 120h, the ICPI value is 0.026, an immunity test proves that the Newcastle disease virus attenuated strain has a good immunity protection effect on Newcastle disease virulent strain; and compared with the conventional vaccine strain LaSota, the NDV-rHR09-LaSota-HN strain is inoculated to one-day SPF chicken, and the antibody level and the protection effect on the prevalent virulent strain counteracting toxic substances of the chicken after three weeks are better or not poorer than a reference group.

The invention belongs to the field of modified additives for polymer materials, in particular to a reactive light stabilizer and a preparation method thereof; for the first time, cinnamate is used as a reactive group to react with hindered amines to synthesize a reactive light stabilizer, stabilizer The benzene ring and the double bond in the cinnamic acid molecule in the light stabilizer form a delocalized π bond, and the benzene ring and the carboxyl group have a suitable space Steric hindrance, which can not only avoid the self-polymerization of the light stabilizer itself, but also ensure the smooth grafting to the polymer main chain; during the synthesis reaction, no polymerization inhibitor is needed, which improves the purity of the product and the difficulty of post-processing ; The raw material cinnamate of the present invention has a wide range of sources, not only can be extracted from plants, but also can be synthesized by chemical methods, safe and environmentally friendly, low in price, and can be widely used in industrial production.

The invention provides an enzyme process acylation method of strawberry anthocyanin, and belongs to the technical filed of deep processing of agricultural products. The method comprises the following steps: extracting strawberry anthocyanin by using edible alcohol, adding the above obtained crude extract liquid into an AB-8 macroporous resin column, preliminarily purifying, extracting fat-soluble impurities by using ethyl acetate, immediately adding the obtained liquid to a polyamide resin column, flushing the polyamide resin column with deionized water, eluting by using an aqueous solution of 30% ethanol, carrying out reduced pressure evaporation on the obtained eluate, dissolving the obtained material into tertiary amyl alcohol, adding cinnamic acid, Novozmy immobilized lipase and 4A molecular sieve, and carrying out a shocking reaction at 40DEG C for 72h, and filtering to remove the above enzyme and the molecular sieve. The method has the advantages of cycle use of the solvent and the enzyme, low cost, suitableness for mass production, and good industrial prospect. Acylated strawberry anthocyanin can be used as an anthocyanin theory research material, and can also be used in different fields as a natural food pigment.

The invention belongs to the technical field of biology, and relates to a herpes simplex virus type 1 carrier system, and a preparation method thereof. The carrier system comprises a shuttle plasmid by taking DNA (deoxyribonucleic acid) fragment at two sides of an inverted repeat sequence (IR) inside HSV-1 (herpes simplex virus type 1) as homologous recombinant arms, and a bacterial artificial chromosome plasmid pHSV delta IR-BAC (bacterial artificial chromosome) containing IR zone deleted HSV-1 genome. The method for preparing recombinant HSV-1 by using the carrier system comprises the following steps: firstly, cloning a target DNA fragment into pKo5/BN to obtain a recombinant shuttle plasmid; secondly, electro-transforming escherichia coli containing pHSV delta IR-BAC by using the recombinant shuttle plasmid, and screening to obtain recombinant bacteria; finally, extracting the recombinant DNA from the recombinant bacteria, transfecting Vero cells, and purifying to obtain recombinant HSV-1. The carrier system is high in security, can accommodate 30Kb of exogenous DNA, and can construct optional replication type recombinant HSV-1 capable of expressing a plurality of target genes.

The present invention relates to the field of pharmaceutical chemistry, and discloses a new regadenoson crystal form, ie., regadenoson crystal form E, and a preparation method of the new regadenoson crystal form. According to the present invention, the regadenoson crystal form E has excellent performances in the field of radionuclide myocardial perfusion imaging, has characteristics of weak toxicity and good storage stability, and can be used for preparing drugs for radionuclide myocardial perfusion imaging stress agents.

The invention discloses a carrier pigeon Newcastle disease virus genetic engineering modified attenuated strain as well as a preparation method and application of the carrier pigeon Newcastle disease virus genetic engineering modified attenuated strain. The method specifically comprises the following steps: carrying out site-directed mutagenesis on amino acids of three cleavage sites of F protein of a carrier pigeon Newcastle disease virus strain (PNDV-YQ strain) by using a reverse genetic manipulation technology, constructing and obtaining a full-length cDNA plasmid pOK-YQ of a low-toxicity gene in a segmented cloning manner, respectively cloning ORF genes of NP, P and L of the PNDV-YQ strain to an eukaryotic expression vector pCIneo, and carrying out expression by using a recombinant vector pCIneo, according to the invention, helper plasmids pCI-NP, pCI-P and pCI-L are constructed and obtained. After a BSR-T7 cell is co-transfected by the full-length plasmid and a helper plasmid, a recombinant attenuated virus, namely the pigeon Newcastle disease virus (PNDV-aYQ strain), is rescued. The strain of virus is a low-pathogenicity or non-pathogenicity strain, and has good proliferation characteristics and stable hereditary characteristics. Compared with the existing newcastle disease vaccine, the inactivated vaccine prepared from the virus strain has the advantages of low side reaction, early immune production period and high protection rate.

The invention relates to a prasugrel hydrochloride ethanol solvate and a preparation method thereof. The prasugrel hydrochloride ethanol solvate and the crystal thereof are defined on the aspects of X-ray powder diffraction pattern, diffraction angle 2 theta degrees, infrared spectroscopy and thermogravimetric curve. The preparation method comprises the following steps of: dissolving prasugrel into a solvent, wherein the ratio of prasugrel to solvent in the solution is 0.08-0.2g/mL; adding hydrochloric acid; filtering; cooling and crystallizing the obtained solid in anhydrous ethanol, wherein the concentration of the initial solution is 0.1 to 0.3g/mL; and filtering the crystal mush, drying, thus obtaining the product. X-ray diffraction (XRD) spectrograms and stereoscan photographs can prove that the crystallinity of the product is high, the granularity of the crystal is large, the surface of the crystal is smooth and clean, the filtering and drying speed is high, the efficiency of the preparation process is improved, and the solvate is beneficial to application to preparation of medicinal preparations.

The strain is a hypotoxic strain with immunogen formed by S6 strain and through thrice solid cloning, then acellular culture and weakening to over 168 generation. The strain is safe, without restoring its strong toxicity, easy to test, and has relatively strong immunogen for preventing chicken's chronic respiratory track disease. The strain is preserved in Common Microbe Center of Chinese Microbial Vaccine Storing and Management Commission with the stroing number being CGMCC No.0397.

The invention discloses a synthesis and modification method of an upconversion nanometer material. The upconversion nanometer material with uniform particle diameter is prepared by a thermolysis method; secondly, mesoporous silica is wrapped outside nanoparticles; surface modification is performed; core-shell nanoparticles are formed; a hydrophylic structure is constructed; finally, photosensitizers and folic acid are carried on porous nanoparticles, so that the functions of photodynamic killing and in vitro targeting on tumor cells are realized. Near-infrared light with high penetrating capability is used for excitation, so that cancer cells generate a large number of active oxygen so as to achieve the effect of inhibiting the cancer; the goal of photodynamic treatment and killing on thetumor cells is realized; the damage to cells and biological tissues is reduced.

The invention discloses a method for preventing and controlling sooty mould of camellia oleifera, which comprises the following steps: (1) pruning infected branches and leaves of camellia oleifera trees from March to April, and carrying out centralized treatment to block insect sources; and smearing a disinfectant at the pruning openings of the pruned branches and leaves for disinfection treatment; (2) throwing ladybirds on the camellia oleifera trees; and (3) spraying imidacloprid liquid medicine to the camellia oleifera forest in May to June and September to October respectively, driving the ladybirds in to-be-sprayed areas to non-sprayed areas before spraying, and driving the ladybirds back to the original sprayed areas when the chemical residues in the to-be-sprayed areas is reduced to a safety value range. According to the method, biological prevention and control and chemical prevention and control are combined, pests with piercing-sucking mouthparts such as aphids, scabies and whiteflies of the camellia oleifera trees are killed by throwing the ladybirds and spraying the insecticide, and the purpose of preventing and controlling the sooty mould is achieved; and according to the method, the prevention and control rate of the sooty mould of the camellia oleifera can be increased to 99% or above, a large amount of insecticide does not need to be sprayed, natural enemies of the pests cannot be killed when the pests are killed, and high biological safety is achieved.