74results about How to "Weak toxicity" patented technology

The invention discloses an ultrasonic coupling agent and application of the ultrasonic coupling agent in ultrasonic inspection or auxiliary treatment of clinical cavity canals, mucous membranes, non-intact skin and the like in hospital. The ultrasonic coupling agent consists of the following raw materials: safe and nontoxic silver ions or nanometer silver serving as a disinfectant, and bio-compatibility macromolecular materials serving as a viscosity regulator, an anti-discoloration agent, an anti-precipitant agent, and an anti-stimulant agent. The ultrasonic coupling agent meets the requirements on ultrasonic impedance and no corrosion, has the characteristics of disinfection, sterilization, safety, nontoxicity, no stimulation and the like, can effectively avoid cross infection in ultrasonic clinics, can be suitable for detecting the cavity canals, the mucous membranes and the non-intact skin, and has high medical clinical value. The ultrasonic coupling agent has a simple preparation method, and low cost of raw materials.

The present invention discloses a coronary artery medicine coating scaffold for inhibiting endometrial hyperplasia and preventing restenosis in the scaffold. It includes dilating scaffold base body and inner coating layer coated on the dilating scaffold base body, the above-mentioned inner coating layer is formed from medicine carrier and ligustrazine, their mass ratio is 3:1-1:3, the above-mentioned inner coating layer is one of methyl acrylate, methyl methacrylate copolymer, polylactic acid, polyglycollic acid, polymonoracemic lactic acid, polylactide-diglycolide polymer, phosphorylcholine, ethyl polyurethane inorganic micropore aluminium oxide and cellulose or their composite.

The invention discloses a method for removing bromine from disperse dye wastewater. The method comprises the following steps: firstly, adjusting the pH value of the disperse dye wastewater to be 2-5, adding a fenton reagent, and reacting at 40-60 DEG C for 0.5-2 hours; adding a flocculant to the wastewater, adjusting into alkalinity, standing and filtering to obtain filtrate I; adding an absorbent to the filtrate I, standing again and filtering to obtain filtrate II; and concentrating the filtrate II and recovering sulphate. According to the method, organic matters and bromine in the disperse dye wastewater can be effectively removed; the removal rate on high-concentration bromine and chromaticity can be up to over 99%; the removal rate of the organic matters can be up to over 75%; excellent sulphate is recovered; and the method is simple and easy to operate, and high in removal rate.

Provided is microbial materials for degradation of oils and toxic chemicals. The microbial material comprises (a) a microorganism and culture filtrate capable of degrading oil and toxic chemicals being at least one selected from the group consisting of Trichosporon loubieri Y1-A of deposit No. KCTC 10876BP, Trichosporon cutaneum, and white-rot fungi living upon the surface of wood, (b) rapeseed oils for producing more sophorolipid in surface of the said microorganism, (c) lipophilic powder being at least one selected from the group consisting of natural wax, synthetic wax, beeswax and waste candle, and (d) a microbial nutrient. This invention further comprises (e) the Bacillus subtilis of Deposit No. KCCM 10639 and the Bacillus subtilis of Deposit No. KCCM 10640. The microbial material can efficiently, rapidly degrade contaminants that are unreadily degradable, by increasing a contact area with the microorganism capable of degrading the unreadily degradable contaminants.

A rapid breeding method of Artemisia annua. Young stems on a single plant or a plurality of plants of Artemisia annua with completely consistent genotype are cut off; the young stems are disinfected in vitro and transversely cut into stem segments, and each stem segment has a bud; and the stem segments are cultured directly in a rooting medium and transplanted for soil culture after rooting. The method of the invention has easily available material, low variation rate, high increment coefficient, short rooting period, a two-week proliferation time reaching higher than 200 times, a rooting rate reaching higher than 90%, a transplanting survival rate reaching higher than 95% and strong factory production capability. Using the method of the invention to breed high-yield Artemisia annua linescan enable progeny to get stable heredity, so as to effectively reduce production cost of artemisinin.

The invention relates to construction of a brucellosis A19 molecular marker vaccine strain and determination of virulence and immunogenicity. According to the invention, a suicide-plasmid-mediated homologous recombination technology is utilized to knock out a virulence factor VirB12 gene of a brucellosis tetratype secretory system of a brucellosis A19 vaccine strain, thus obtaining the A19-delta VirB12 molecular marker vaccine strain. Animal experiments show that the virulence of the A19 molecular marker vaccine strain is slightly weaker than that of a parent A19 vaccine strain, so that the strain safety is further improved, but the good immune effect on brucellosis is maintained; and moreover, the molecular marker vaccine strain has stable heredity. According to the invention, the brucellosis VirB12 gene is taken as a molecular marker, a polymerase chain reaction (PCR) method is applied to identify the brucellosis vaccine strain from wild viral strains, so that the molecular marker is provided for building a determination method for identifying vaccine immunization and natural infection, and the function of the original brucellosis A19 vaccine is improved, therefore, the brucellosis A19 molecular marker vaccine strain has an very important practical meaning on controlling the epidemic brucellosis, and has wide application prospect.

The present invention relates to a hyaluronic acid-nucleic acid conjugate for the development of in vivo nucleic acid delivery system, and the development of nucleic acid delivery system using the same. Specifically, a hyaluronic acid-nucleic acid complex wherein a hyaluronic acid-alkylenediamine conjugate and nucleic acid are connected by a disulfide bond; a composition for nucleic acid delivery comprising the hyaluronic acid-nucleic acid complex as an active ingredient; a method for preparing the hyaluronic acid-nucleic acid complex; and a method for in vivo delivery of nucleic acid, comprising administering the hyaluronic acid-nucleic acid complex to a subject are provided.

The invention relates to the technical fields of veterinarian virus molecule biology and immunity, and discloses a recombinant gene VIII-type Newcastle disease virus attenuated strain NDV-rHR09-LaSota-HN. A preservation number of the attenuated strain NDV-rHR09-LaSota-HN is CCTCC (China Center For Type Culture Collection) NO: V201830. The research shows that the NDV-rHR09-LaSota-HN strain is weakin toxicity, the MDT is greater than 120h, the ICPI value is 0.026, an immunity test proves that the Newcastle disease virus attenuated strain has a good immunity protection effect on Newcastle disease virulent strain; and compared with the conventional vaccine strain LaSota, the NDV-rHR09-LaSota-HN strain is inoculated to one-day SPF chicken, and the antibody level and the protection effect on the prevalent virulent strain counteracting toxic substances of the chicken after three weeks are better or not poorer than a reference group.

The invention relates to a polypeptide for restraining EGF induced tumor cell proliferation through targeted EGFR and belongs to the technical field of biological medicines. According to the invention, a biological active polypeptide TUZG20 is acquired by taking EGF (Epidermal Growth Factor) as a target molecule, elutriating by adopting a phage display technology, taking EGF as an active ingredient, specifically eluting and combining with the phage of the target molecule. The invention proves that the biological active polypeptide TUZG20 is capable of obviously restraining the gastric carcinoma cell proliferation process caused by EGF. The polypeptide provided by the invention has a short sequence, is easy for compounding and large-scale production, is capable of restraining the tumor-promoting action of EGF, has a potential of subsequently developing into anti-cancer drugs and has an important application value at the aspect of research and development of anti-cancer drugs.

The invention belongs to the field of modified additives for polymer materials, in particular to a reactive light stabilizer and a preparation method thereof; for the first time, cinnamate is used as a reactive group to react with hindered amines to synthesize a reactive light stabilizer, stabilizer The benzene ring and the double bond in the cinnamic acid molecule in the light stabilizer form a delocalized π bond, and the benzene ring and the carboxyl group have a suitable space Steric hindrance, which can not only avoid the self-polymerization of the light stabilizer itself, but also ensure the smooth grafting to the polymer main chain; during the synthesis reaction, no polymerization inhibitor is needed, which improves the purity of the product and the difficulty of post-processing ; The raw material cinnamate of the present invention has a wide range of sources, not only can be extracted from plants, but also can be synthesized by chemical methods, safe and environmentally friendly, low in price, and can be widely used in industrial production.

The present invention relates to organism tissue staining method with RE fluorescent complex. The staining reagent used in the present invention is solution of RE fluorescent complex, and the solution is dropped onto the organism tissue or the test sample is soaked in the solution for 0.5-3 min before flushing with distilled water. Under the exciting of ultraviolet light, the configuration and detailed structure of the stainless tissue sample may be observed in fluorescent microscope. The said method has simple operatino and short period, and the sample needs no pre-treatment and unlimited. The reagent has no or only weak toxicity and the staining process has no influence on the structure of tissue or cell. The tissue may be extracorporeal or intracorporeal.

The invention provides an enzyme process acylation method of strawberry anthocyanin, and belongs to the technical filed of deep processing of agricultural products. The method comprises the following steps: extracting strawberry anthocyanin by using edible alcohol, adding the above obtained crude extract liquid into an AB-8 macroporous resin column, preliminarily purifying, extracting fat-soluble impurities by using ethyl acetate, immediately adding the obtained liquid to a polyamide resin column, flushing the polyamide resin column with deionized water, eluting by using an aqueous solution of 30% ethanol, carrying out reduced pressure evaporation on the obtained eluate, dissolving the obtained material into tertiary amyl alcohol, adding cinnamic acid, Novozmy immobilized lipase and 4A molecular sieve, and carrying out a shocking reaction at 40DEG C for 72h, and filtering to remove the above enzyme and the molecular sieve. The method has the advantages of cycle use of the solvent and the enzyme, low cost, suitableness for mass production, and good industrial prospect. Acylated strawberry anthocyanin can be used as an anthocyanin theory research material, and can also be used in different fields as a natural food pigment.

The invention relates to the field of skin care products, and particularly provides application of a leech peptide HE-D in skin care and whitening. The invention provides the following application of leech peptide HE-D: inhibiting tyrosinase activity, inhibiting cell melanin generation, beautifying and whitening, and preparing essence, toner or body lotion. The HE-D as a polypeptide has good stability and weak toxicity to cells, is easily absorbed by a human body as a natural active peptide, has high efficiency of inhibiting tyrosinase activity and melanin generation, can be widely applied to related fields of skin care products, and can be used as a reagent for scientific research.

The invention relates to a method for improving the specificity of cryptosporidium hominis in water and a method for evaluating the activity of cryptosporidium hominis in water. The method is used for evaluating the activity of cryptosporidium hominis in water by utilizing the fluorescent quantitative PCR (polymerase chain reaction) (qPCR) technology in combination with an azide colorant propidium monoazide (PMA), and comprises the steps of irradiating a sample to which a PMA dye is added by strong light, constructing a target gene and primers, carrying out qPCR reaction and detecting cryptosporidium hominis with activity. By adopting the method, the sensitivity and specificity of detection are improved, the operating steps are simplified, the detection time is shortened, and the detection cost is reduced.

The invention discloses a novel neutralizing antibody detection method based on a rescued recombinant attenuated rabies virus. The inventor rescues a recombinant virus rRV-eGFP with green fluorescent protein by utilization of a reverse genetics technology and improves the internationally accepted RFFIT method for valence detection of a neutralizing antibody against rabies virus by adoption of the recombinant virus, thus developing an effective modified RFFIT-eGFP method. The novel neutralizing antibody detection method improves detection effects of the RFFIT method, and has characteristics of good consistency, high specificity, high safety, no need of expensive primary antibodies or FITC-labeled second antibodies, capability of being more rapid, simpler and more convenient, and the like when compared with the standard RFFIT method. The novel neutralizing antibody detection method overcomes the problems, namely use of a challenge virus standard strain that is a CVS-11 strain with high virulence, long consumed time, and the like of the traditional RFFIT method.

The invention discloses a device for thin film overlying photovoltaic and quick thermal treatment method so disposed that the method comprises a transparent substrate including a surface region. A first electrode layer overlies the surface region. A copper layer is formed overlying the first electrode layer and indium layer is formed overlying the copper layer to form a multi-layered structure. The multi-layered structure is subjected to a thermal treatment process in an environment containing a sulfur bearing species to form a bulk copper indium disulfide. the quick thermal treatment employing a gradient ranging between 1 degree centigrade per second to about 50 degrees centigrade per second. In specific embodiment, the resistivity of the first transparent electrode layer is kept of about 10 ohm / cm2 or less, and the light transmission rate is more than 80 percent.

The invention belongs to the technical field of biology, and relates to a herpes simplex virus type 1 carrier system, and a preparation method thereof. The carrier system comprises a shuttle plasmid by taking DNA (deoxyribonucleic acid) fragment at two sides of an inverted repeat sequence (IR) inside HSV-1 (herpes simplex virus type 1) as homologous recombinant arms, and a bacterial artificial chromosome plasmid pHSV delta IR-BAC (bacterial artificial chromosome) containing IR zone deleted HSV-1 genome. The method for preparing recombinant HSV-1 by using the carrier system comprises the following steps: firstly, cloning a target DNA fragment into pKo5 / BN to obtain a recombinant shuttle plasmid; secondly, electro-transforming escherichia coli containing pHSV delta IR-BAC by using the recombinant shuttle plasmid, and screening to obtain recombinant bacteria; finally, extracting the recombinant DNA from the recombinant bacteria, transfecting Vero cells, and purifying to obtain recombinant HSV-1. The carrier system is high in security, can accommodate 30Kb of exogenous DNA, and can construct optional replication type recombinant HSV-1 capable of expressing a plurality of target genes.

The present invention relates to the field of pharmaceutical chemistry, and discloses a new regadenoson crystal form, ie., regadenoson crystal form E, and a preparation method of the new regadenoson crystal form. According to the present invention, the regadenoson crystal form E has excellent performances in the field of radionuclide myocardial perfusion imaging, has characteristics of weak toxicity and good storage stability, and can be used for preparing drugs for radionuclide myocardial perfusion imaging stress agents.

The invention relates to a Salmonella typhi gene deletion strain, a vaccine prepared from the Salmonella typhi gene deletion strain and application of the strain. The bacterial strain is Salmonella typhi delta crpST-1 and preserved in the China center for type culture collection, and the preservation number is CCTCC NO: M2011373. The Salmonella typhi gene deletion strain disclosed by the invention has excellent immunizing protection efficacy and weak toxicity without any adverse side effect on immunized pigs and is safer compared with the traditional vaccine. Therefore, the vaccine which is prepared from the gene deletion strain built by a parent strain has wide market application prospect. In addition, the Salmonella typhi gene deletion strain disclosed by the invention retains the immune protective efficacy aiming to salmonella typhi infection, and has clear genetic background and stable characteristics, so that the Salmonella typhi gene deletion strain has better biosecurity. The Salmonella typhi gene deletion strain disclosed by the invention does not contain a resistance tag and totally accords with the biological security requirement of vaccine.

The invention discloses a carrier pigeon Newcastle disease virus genetic engineering modified attenuated strain as well as a preparation method and application of the carrier pigeon Newcastle disease virus genetic engineering modified attenuated strain. The method specifically comprises the following steps: carrying out site-directed mutagenesis on amino acids of three cleavage sites of F protein of a carrier pigeon Newcastle disease virus strain (PNDV-YQ strain) by using a reverse genetic manipulation technology, constructing and obtaining a full-length cDNA plasmid pOK-YQ of a low-toxicity gene in a segmented cloning manner, respectively cloning ORF genes of NP, P and L of the PNDV-YQ strain to an eukaryotic expression vector pCIneo, and carrying out expression by using a recombinant vector pCIneo, according to the invention, helper plasmids pCI-NP, pCI-P and pCI-L are constructed and obtained. After a BSR-T7 cell is co-transfected by the full-length plasmid and a helper plasmid, a recombinant attenuated virus, namely the pigeon Newcastle disease virus (PNDV-aYQ strain), is rescued. The strain of virus is a low-pathogenicity or non-pathogenicity strain, and has good proliferation characteristics and stable hereditary characteristics. Compared with the existing newcastle disease vaccine, the inactivated vaccine prepared from the virus strain has the advantages of low side reaction, early immune production period and high protection rate.

The invention relates to a prasugrel hydrochloride ethanol solvate and a preparation method thereof. The prasugrel hydrochloride ethanol solvate and the crystal thereof are defined on the aspects of X-ray powder diffraction pattern, diffraction angle 2 theta degrees, infrared spectroscopy and thermogravimetric curve. The preparation method comprises the following steps of: dissolving prasugrel into a solvent, wherein the ratio of prasugrel to solvent in the solution is 0.08-0.2g / mL; adding hydrochloric acid; filtering; cooling and crystallizing the obtained solid in anhydrous ethanol, wherein the concentration of the initial solution is 0.1 to 0.3g / mL; and filtering the crystal mush, drying, thus obtaining the product. X-ray diffraction (XRD) spectrograms and stereoscan photographs can prove that the crystallinity of the product is high, the granularity of the crystal is large, the surface of the crystal is smooth and clean, the filtering and drying speed is high, the efficiency of the preparation process is improved, and the solvate is beneficial to application to preparation of medicinal preparations.

The invention relates to a method for preparing a 1,3-dioxane acetal type dianion surface active agent, which comprises the following steps of: reacting n-heptaldehyde with pentaerythritol to obtain 1,3-dioxane acetal and then reacting the 1,3-dioxane acetal with 1-chlorol-3-bromopropane; and reacting the obtained product with potassium iodide, sodium sulfite and catalyst tetrabutyl ammonium bromide in water to prepare the product. The used sulfonated reagent sodium sulfite has wide industrial source, low cost and low toxicity to the human body and provides the condition for realizing the industrialization of the product.

The invention discloses a magnetic multi-sensibilization sensor for aflatoxin as well as preparation and application of the magnetic multi-sensibilization sensor. The nano enzyme (A) is formed by modifying ferroferric oxide with histidine; the capture probe (B) is formed by coupling a nano-enzyme with a monoclonal antibody of aflatoxin B1 (AFB1); the detection probe (C) is formed by coupling a nano-enzyme with an antigen of AFB1; the capture probe and the detection probe form a two-dimensional net structure (D) through specific binding between antigens and antibodies. The method comprises the following steps: preparing magnetic nano-enzyme for later use; modifying the capture probe; modifying the detection probe; and forming a two-dimensional net structure. In the detection process, after the constructed two-dimensional network structure is subjected to magnetic separation and enrichment, a 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) color developing reagent is added, and the two-dimensional network structure catalyzes the substrate to develop color. The magnetic multi-sensibilization detection method established by the invention has the advantages of high enrichment degree, high sensitivity, rapidness and the like, and is suitable for large-scale screening detection of AFB1 in food.

The strain is a hypotoxic strain with immunogen formed by S6 strain and through thrice solid cloning, then acellular culture and weakening to over 168 generation. The strain is safe, without restoring its strong toxicity, easy to test, and has relatively strong immunogen for preventing chicken's chronic respiratory track disease. The strain is preserved in Common Microbe Center of Chinese Microbial Vaccine Storing and Management Commission with the stroing number being CGMCC No.0397.

The invention discloses a synthesis and modification method of an upconversion nanometer material. The upconversion nanometer material with uniform particle diameter is prepared by a thermolysis method; secondly, mesoporous silica is wrapped outside nanoparticles; surface modification is performed; core-shell nanoparticles are formed; a hydrophylic structure is constructed; finally, photosensitizers and folic acid are carried on porous nanoparticles, so that the functions of photodynamic killing and in vitro targeting on tumor cells are realized. Near-infrared light with high penetrating capability is used for excitation, so that cancer cells generate a large number of active oxygen so as to achieve the effect of inhibiting the cancer; the goal of photodynamic treatment and killing on thetumor cells is realized; the damage to cells and biological tissues is reduced.

The invention discloses a method for preventing and controlling sooty mould of camellia oleifera, which comprises the following steps: (1) pruning infected branches and leaves of camellia oleifera trees from March to April, and carrying out centralized treatment to block insect sources; and smearing a disinfectant at the pruning openings of the pruned branches and leaves for disinfection treatment; (2) throwing ladybirds on the camellia oleifera trees; and (3) spraying imidacloprid liquid medicine to the camellia oleifera forest in May to June and September to October respectively, driving the ladybirds in to-be-sprayed areas to non-sprayed areas before spraying, and driving the ladybirds back to the original sprayed areas when the chemical residues in the to-be-sprayed areas is reduced to a safety value range. According to the method, biological prevention and control and chemical prevention and control are combined, pests with piercing-sucking mouthparts such as aphids, scabies and whiteflies of the camellia oleifera trees are killed by throwing the ladybirds and spraying the insecticide, and the purpose of preventing and controlling the sooty mould is achieved; and according to the method, the prevention and control rate of the sooty mould of the camellia oleifera can be increased to 99% or above, a large amount of insecticide does not need to be sprayed, natural enemies of the pests cannot be killed when the pests are killed, and high biological safety is achieved.

The invention belongs to the field of positive electrode materials for lithium ion batteries, and particularly relates to a method for preparing a graphene composite ternary material by a monovalent alcohol solvothermal method. Method for preparing graphene composite ternary material by monovalent alcohol solvothermal method, the ternary material is LiNixCoyMnzO2, x+y+z=1, 0.2≤x≤0.6, 0.1≤y≤0.4, 0.2≤z≤0.5; graphite The general chemical formula of the ene composite ternary material is Graphene / LiNixCoyMnzO2. The advantages of the present invention are: 1) Since the ternary material LiNixCoyMnzO2 is sensitive to water, it is a good choice for a monohydric alcohol to replace water as a solvent, because the temperature is less than 220℃ , when the pressure is not more than 3 MPa, the monohydric alcohol is easy to form a subcritical state, which is non-flammable, cheap, and weak in toxicity; 2) The graphene composite ternary cathode material prepared by the present invention has excellent electrochemical performance, and its rate performance is better than traditional 3) The preparation process is relatively simple, the cost is low, and it is suitable for large-scale industrial production.

The reptilase is extracted from snake poison of pallas pit viper, and its SDS-polyacrylamide gel electrophoresis exhibits three bands of molecular weight 54000 D, 34000 D and 15000 D separately. The reptilase producing process of the present invention includes the following steps: taking snake poison of pallas pit viper, dissolving in buffering liquid, centrifugal deposition, elution in saturatedphenol solution to obtain the grade-I reptilase product; dissolving the grade-I reptilase product in buffering liquid, ion exchange column chromatography and elution with buffering liquid to obtain refined reptilase liquid. The reptilase medicine of the present invention has very low toxicity, safe clinical application and wide application foreground.

The invention relates to a prasugrel hydrochloride ethanol solvate and a preparation method thereof. The prasugrel hydrochloride ethanol solvate and the crystal thereof are defined on the aspects of X-ray powder diffraction pattern, diffraction angle 2 theta degrees, infrared spectroscopy and thermogravimetric curve. The preparation method comprises the following steps of: dissolving prasugrel into a solvent, wherein the ratio of prasugrel to solvent in the solution is 0.08-0.2g / mL; adding hydrochloric acid; filtering; cooling and crystallizing the obtained solid in anhydrous ethanol, wherein the concentration of the initial solution is 0.1 to 0.3g / mL; and filtering the crystal mush, drying, thus obtaining the product. X-ray diffraction (XRD) spectrograms and stereoscan photographs can prove that the crystallinity of the product is high, the granularity of the crystal is large, the surface of the crystal is smooth and clean, the filtering and drying speed is high, the efficiency of the preparation process is improved, and the solvate is beneficial to application to preparation of medicinal preparations.