The invention relates to the technical field of nano-material preparing, in particular to a preparing method of nano-hydroxyapatite. According to the preparing method, firstly birch bark fiber is decomposed through alkali boiling, later microorganism degradation intake is better facilitated, acetobacter xylinum is cultured, bacterial cellulose is obtained, and a calcium ion precursor is anchored to nano bacterial fiber; next, under the effect of ammonium hydroxide, phospholipid and lecithin are hydrolyzed, the hydrolysis product, calcium ions anchored to the surface of a bacterial cellulose template, nano-hydroxyapatite ions and hydroxyl groups are slowly combined to generate crystal nucleuses, as time passes, the crystal nucleuses gradually grow, the nanoscale space of the bacterial cellulose template can stop agglomeration of the nano-hydroxyapatite ions, by controlling the pH and adding citric acid, the purpose of lowering the concentration of calcium ions is achieved, the aqueous phase homogeneous precipitation kinetic constant is changed, in the in-situ crystallization process, nano-hydroxyapatite with a small size tends to be formed, and nano-hydroxy calcium phosphate is obtained after calcination. The preparing method of nano-hydroxy calcium phosphate is wide in application prospect.