Preparing method of nano-hydroxyapatite
A nano-hydroxyapatite and a technology for preparation steps are applied in the field of preparation of nano-hydroxyapatite, and can solve the problems of failing to meet nano-scale requirements, uneven particle size of hydroxyapatite, etc. The effect of reducing calcium ion concentration
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example 1
[0025] According to the mass ratio of 1:10, weigh birch bark and 10% sodium hydroxide solution, mix them, heat and boil for 3 hours, filter and separate to obtain the filter residue, and rinse with 10% citric acid solution and deionized water in turn. 10min to obtain the pretreatment filter residue; in parts by weight, weigh 30 parts of the pretreatment filter residue, 20 parts of glucose, 5 parts of peptone, 5 parts of yeast powder, 2 parts of disodium hydrogen phosphate and 100 parts of distilled water and put them into high-speed homogeneous dispersion In the machine, disperse and homogenize at a speed of 3000r / min for 20 minutes to obtain a dispersed slurry, put it into a high-temperature sterilization box and sterilize it at 120°C for 30 minutes to obtain a sterilized slurry; put the sterilized slurry into a fermenter, Add the concentration of 10% of sterilizing slurry quality in fermentor again and be 10 6 Acetobacter xylinum suspension of cfu / mL, seal the mouth of the t...
example 2
[0027]According to the mass ratio of 1:10, weigh the birch bark and 10% sodium hydroxide solution, mix them, heat and boil for 4 hours, filter and separate to obtain the filter residue, and wash them with 10% citric acid solution and deionized water in turn. 13min to obtain the pretreatment filter residue; in parts by weight, weigh 35 parts of pretreatment filter residue, 23 parts of glucose, 8 parts of peptone, 7 parts of yeast powder, 3 parts of disodium hydrogen phosphate and 130 parts of distilled water into high-speed homogeneous dispersion In the machine, disperse and homogenize at a speed of 3500r / min for 25 minutes to obtain a dispersed slurry, put it in a high-temperature sterilization box and sterilize it at 120°C for 32 minutes to obtain a sterilized slurry; put the sterilized slurry into a fermenter, Add the concentration of 10% of sterilizing slurry quality in fermentor again and be 10 6 Acetobacter xylinum suspension of cfu / mL, seal the mouth of the tank, and fer...
example 3
[0029] According to the mass ratio of 1:10, weigh birch bark and 10% sodium hydroxide solution, mix them, heat and boil for 5 hours, filter and separate to obtain the filter residue, and rinse with 10% citric acid solution and deionized water respectively 15min to obtain the pretreatment filter residue; in parts by weight, weigh 40 parts of pretreatment filter residue, 25 parts of glucose, 10 parts of peptone, 8 parts of yeast powder, 5 parts of disodium hydrogen phosphate and 150 parts of distilled water into high-speed homogeneous dispersion In the machine, disperse and homogenize at a speed of 4000r / min for 30 minutes to obtain a dispersed slurry, put it into a high-temperature sterilization box and sterilize it at 120°C for 40 minutes to obtain a sterilized slurry; put the sterilized slurry into a fermenter, Add the concentration of 10% of sterilizing slurry quality in fermentor again and be 10 6 cfu / mL of Acetobacter xylinum suspension, seal the mouth of the tank, and fer...
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