67 results about "Salivary Proteins" patented technology

The relates to an agglutinin chip used for identifying cancer, in particular to an agglutinin chip for identifying breast cancer based on sialoprotein, a reagent kit and application of the reagent kit, and aims at providing an agglutinin chip for identifying breast cancer in a non-destructive mode through the change of carbohydrate chains in saliva, a reagent kit and application of the reagent kit. The agglutinin chip for identifying breast cancer based on sialoprotein comprises an agglutinin testing probe set. The agglutinin testing probe set at least comprises a combination of PHA-E+L, LTL, BS-I, MAL-I, LCA, BPL, PTL-II, NPA and GSL-I, or at least comprises a combination of LTL, BS-I, MAL-I, LCA, BPL, NPA and GSL-I. By means of the agglutinin chip, differentially-expressed glycoprotein carbohydrate chain structures in a patient saliva sample can be rapidly detected, whether a corresponding person suffers from a breast tumor or not is detected, and whether the breast tumor is breast cancer or not is determined; besides, saliva is adopted as an object to be detected and is easy and convenient to collect, and health risks existing when a serum sample is collected for detection in the prior art are avoided.

The invention discloses a salivary protein diagnosis model for gastric cancer and an establishment method for the salivary protein diagnosis model. The establishment method for the salivary protein diagnosis model for gastric cancer comprises the following steps of collecting and processing a saliva sample; processing the saliva sample by adopting WCX magnetic balls; performing sample application and mass spectrometry; and performing data statistical analysis. The salivary protein diagnosis model for gastric cancer mainly comprises four salivary protein peaks, and mass-to-charge (m / z) ratios of the salivary protein peaks in human salivary proteins are 1,472.78Da, 2,936.49Da, 6,556.81Da and 7,081.17Da respectively. According to the salivary protein diagnosis model for gastric cancer and the establishment method for the salivary protein diagnosis model, by a classification prediction model established by the four protein peaks of which the mass-to-charge ratios (m / z) in the human salivary proteins are 1,472.78Da, 2,936.49Da, 6,556.81Da and 7,081.17Da respectively, the m / z of the corresponding proteins in human saliva and the model are analyzed to preliminarily judge gastric cancer, the identification rate is 97.83 percent, and the predication capability is 79.82 percent. Clinical back substitution test results show that the accuracy rate of the diagnosis model is 97.56 percent, the sensitivity of the diagnosis model is 95.65 percent and the specificity of the diagnosis model is 100 percent. The establishment method is simple, and has the characteristics of reasonability, feasibility, convenience in operation and batch processing capability.

A method of differentiating among the presence of ductal carcinoma in situ in the breast, benign fibroadenoma of the breast, and non-cancerous breast tissue in a subject is disclosed. The method comprises: measuring the concentration of at least one protein biomarker selected from a group of forty-nine differentially expressed proteins in the saliva of persons with DCIS, or benign fibroadenoma, or in persons who are cancer-free. The resulting test data is compared to a reference panel. From the comparison the presence in the subject of either ductal carcinoma in situ of the breast, or benign fibroadenoma of the breast is determined.

The invention provides a lectin chip for detecting carbohydrate chain markers based on protein in blood serum or saliva as well as a kit and application of the lectin chip, relates to the lectin chip for detecting the carbohydrate chain markers in blood serum or saliva, and particularly relates to the lectin chip for detecting the carbohydrate chain markers based on protein in blood serum and saliva as well as a kit and application of the lectin chip. The invention aims at providing a non-injury lectin chip for detecting the carbohydrate chain markers by identifying protein in saliva or blood serum and a method thereof. The lectin chip for detecting the carbohydrate chain markers based on serum carbohydrate binding protein, provided by the invention, comprises a testing lectin probe set, wherein the testing lectin probe set at least comprises PTL-I, PNA, AAL, LTL, STL, BS-I, PTL-II, SBA, ACA, UEA-I, MAL-I and PHA-E+L. The lectin chip can be used for rapidly detecting a specific carbohydrate protein chain structure in blood serum and saliva samples, and rapidly judging the specific combination of carbohydrate chains in the samples, so as to determine whether corresponding people have hepatitis or not and determine whether people are ACHBLF patients or not.

Provided herein is an all-in-one saliva collection apparatus that collects saliva to allow for the filtration of saliva in order to separate saliva components, such as extracellular proteins and nucleic acids that are not present in intact cells, from the intact cells and debris remaining in the extracted sample. The filtered saliva samples can be aliquoted into two fractions for protein and / or nucleic acid analysis. The present invention further describes long term storage at ambient temperatures of filtered salivary nucleic acids, and long term storage at ambient temperatures of filtered salivary proteins added to an ethanol solution. The filtered cell-free saliva samples have diagnostic usefulness.

The invention provides a transgenic animal having within its genome a transgene construct for gastrointestinal tract specific expression of a protein. In a preferred embodiment, the protein is a phytase or a homologue thereof. Such proteins may be heterologous and may be specifically expressed in the salivary gland of the animal by operably linking the nucleic acid sequence encoding the protein with regulatory sequence including a salivary gland protein promoter / enhancer. Also provided are methods of expressing and producing proteins using such nucleic acid constructs. Further, antibodies specific to such proteins and immunological diagnostic kits are also provided.

The present invention provides for the first time the identification of salivary protein and RNA factors that can be used in the detection of diabetes. The present invention therefore provides methods of diagnosing and providing a prognosis for diabetes, by examining relevant proteins and RNA in a patient's saliva.

The present invention provides Novel collagen-binding peptides of bone sialoprotein (BSP). Peptides comprising a portion of the N-terminal collagen binding domain of BSP (residues 1-100) are used to stimulate mineralization, nucleate hydroxyapatite, and promote bone formation in collagen expressing tissues. Medicaments for use in the same are also contemplated. Chimeric and conjugate peptides comprising said collagen-binding BSP peptides are also included.

The invention discloses a bionic anti-caries functional polypeptide based on casein-rich saliva, a polypeptide derivative or a pharmaceutically acceptable salt and an application in pharmacy thereof. The bionic anti-caries functional polypeptide is based on an anti-caries functional segment of the casein-rich saliva, the adjustment and improvement for the amino acid sequence are performed on the anti-caries functional segment and the bionic anti-caries functional polypeptide contains the amino acid sequence shown as SEQ ID NO.1. The polypeptide mainly utilizes the 'DpSpSEEK' amino acid sequence to realize the adsorption combination of polypeptide and de-mineralized enamel and the 'DpSpSEEK' amino acid sequence thereof can react with calcium phosphate ions so as to induce hydroxyapatite nucleation, so that the in-situ mineralizing repairing function for early enamel caries can be realized. The polypeptide is characterized by lower molecular weight, convenience in compound, low cost, better anti-caries effect, structure stability, safety and reliability. The invention utilizes the bionic thought to simulate natural saliva protein, designs the anti-caries polypeptide with a re-mineralization function and provides an ideal new method for preventing and controlling caries.

Provided herein is an all-in-one saliva collection apparatus that collects saliva to allow for the filtration of saliva in order to separate saliva components, such as extracellular proteins and nucleic acids that are not present in intact cells, from the intact cells and debris remaining in the extracted sample. The filtered saliva samples can be aliquoted into two fractions for protein and / or nucleic acid analysis. The present invention further describes long term storage at ambient temperatures of filtered salivary nucleic acids, and long term storage at ambient temperatures of filtered salivary proteins added to an ethanol solution. The filtered cell-free saliva samples have diagnostic usefulness.

The invention belongs to the technical field of agricultural pest prevention and control, and particularly relates to an apis cerana sialoprotein RP402 and application thereof. The invention provides the sequence of the salivary protein RP402 of the point bug and the coding gene of the salivary protein RP402, and the RP402 gene is silent in a specific targeting manner through an RNA (Ribonucleic Acid) interference technology, so that the inhibition of a soybean aging process can be obviously relieved, the normal aging of leaves is accelerated, and the occurrence of a green symptom phenomenon is reduced, thereby effectively improving the normal ripening rate and the yield of soybeans. Therefore, the prevention and control method for the Eriomycorus lividus or the prevention and control method for the soybean disease green provided by the invention has the characteristics of accuracy, high efficiency and environmental protection, not only makes up for the defects of the existing prevention and control measures, but also reduces the use of chemical pesticides, reduces the environmental pollution and the risk of pest resistance to drugs, and improves the economic benefits of the Eriomycorus lividus and the soybean disease green. Popularization and application of the method can provide powerful guarantee for green and sustainable development of the soybean industry in China.

The invention provides a transgenic animal having within its genome a transgene construct for gastrointestinal tract specific expression of a protein. In a preferred embodiment, the protein is a phytase or a homologue thereof. Such proteins may be heterologous and may be specifically expressed in the salivary gland of the animal by operably linking the nucleic acid sequence encoding the protein with regulatory sequence including a salivary gland protein promoter / enhancer. Also provided are methods of expressing and producing proteins using such nucleic acid constructs. Further, antibodies specific to such proteins and immunological diagnostic kits are also provided.

The present invention provides for the first time the identification of salivary protein and RNA factors that can be used in the detection of primary Sjögren's Syndrome. The present invention therefore provides methods of diagnosing and providing a prognosis for Sjögren's Syndrome, by examining relevant proteins (including certain autoantigens and autoantibodies) and RNA in a patient's saliva.

The invention discloses a method for detecting the content of an SA alpha 2-3Gal sugar chain in a saliva sample by ELISA. According to the method, a fetal globulin is used as a standard substance, andthe content of the fetal globulin which has the same content as the SA alpha 2-3Gal sugar chain in the saliva sample under a certain saliva protein coating concentration can be calculated by utilizing a standard curve, so that the content of the SA alpha 2-3Gal sugar chain in the saliva sample can be calculated, and the quantitative result is accurate.

The invention discloses a detection method of diabetes B kidney deficiency salivary protein markers. The method comprises the following concrete steps of extracting sample protein; determining the extracted protein concentration by a Bradford method; performing proteolysis, iTRAQ marking, vacuum freeze centrifugation drying and reversed-phase chromatography separation under high pH conditions; performing protein analysis on ABI-5600; performing mass spectrometric data analysis; during database selection, if organisms are sequenced organisms, directly selecting the species databases; if the organisms are non-sequenced organisms, selecting main category proteome database most relevant to the tested sample; using an ABI-5600 type mass spectrograph to perform iTRAQ mass spectrograph analysis.The specific protein marker in the diabetes B kidney deficiency can be fast and reliably discovered by a proteomic technological scheme. A noninvasive, simple, convenient, fast and practical detectionand evaluation measure is built for the early diagnosis and treatment, postoperative recurrence, transfer and prognosis observation of the diabetes B kidney deficiency.

A method of diagnosing a patient's risk of breast cancer comprises measuring in a saliva sample from the patient a concentration of at least a first protein marker, wherein the first protein marker is either ubiquitin or cytochrome p450, to provide a set of test data comprising a concentration value of each protein marker in the saliva sample. The test values are then compared to a reference panel comprising a Reference Control Value and a Reference Breast Cancer Value, and a diagnosis of either increased or decreased risk of breast cancer is determined for the patient based on a result of that comparison. A test kit for identifying a person at increased risk of breast cancer is also provided.

A method of differentiating among the presence of ductal carcinoma in situ in the breast, benign fibroadenoma of the breast, and non-cancerous breast tissue in a subject is disclosed. The method comprises: measuring the concentration of at least one protein biomarker selected from a group of forty-nine differentially expressed proteins in the saliva of persons with DCIS, or benign fibroadenoma, or in persons who are cancer-free. The resulting test data is compared to a reference panel. From the comparison the presence in the subject of either ductal carcinoma in situ of the breast, or benign fibroadenoma of the breast is determined.

The invention discloses a method for preparing a saliva protein sample, and belongs to the technical field of preparation of saliva protein samples. The method is characterized by comprising the following steps of: 1) collecting saliva; 2) centrifuging 800g of collected saliva at 4 DEG C for 15 minutes, and sucking supernate; 3) adding 0.05 to 5 volume percent acetic acid and uniformly mixing; 4)centrifuging 14,000g of the mixed solution at 4 DEG C for 60 minutes, and sucking supernate; 5) putting the supernate in a dialysis bag, and dialyzing by using double distilled water for 5 hours; and6) condensing to obtain the saliva protein sample. The method has the advantages of high protein recovery rate, short preparation time, safety, nontoxicity, increase of loading quantity of low abundance proteins, clear and easily analyzed maps and the like.

The present invention provides a novel therapy concept based on a removal of circulation BSP (bone sialoprotein) from the plasma of patients with chronic kidney disease (CKD), preferably by plasmapheresis or an administration of antibodies against BSP in plasma. The present invention further provides a BSP absorber material for plasmapheresis and a pharmaceutical composition namely in form of anti-BSP antibodies for direct administration which are biocompatible in humans. The beneficial effects of this therapy have been proven by the observed correspondence between levels of circulating free BSP levels and mortality of CKD patients.

The invention discloses a sialoprotein gene for regulating and controlling the growth and development of brown planthoppers as well as dsRNA and application thereof. The nucleotide sequence of the sialoprotein gene SISP is shown as SEQ ID NO.1; the dsRNA sequence of the sialoprotein gene for regulating and controlling the growth and development of the brown planthopper is as shown in SEQ ID NO.3. According to the dsRNA disclosed by the invention, SISP can be effectively silenced, the dsRNA is prepared into a nucleic acid pesticide to be applied to brown planthopper, the inhibition of the brown planthopper on the insect resistance of rice can be reduced, and the dsRNA has a serious adverse effect on normal feeding, survival and reproduction of the brown planthopper. The method has the advantages of no pollution and reduction of the use amount of chemical pesticides. Besides, the SISP gene is overexpressed in the rice, so that the content of lignin and flavonoid compounds in the rice can be remarkably reduced, and the insect resistance of the rice is reduced.

The invention discloses a method for detecting mosquito salivary protein specific immune globulin E in animal serum through a capture method enzyme-linked immunosorbent assay, and belongs to the technical field of immunodetection. The monoclonal antibody specifically combined with the human or mouse IgE Fc fragment is used as the capture antibody, and the V5 tag is combined, so that the interference of IgG in a detection sample can be avoided, and the detection sensitivity breaks through the limit of an existing detection mode.

The invention relates to the technical field of agricultural biology, in particular to cotton bollworm salivary protein Ha22, a recombinant vector, recombinant bacteria and application of the cotton bollworm salivary protein Ha22. The invention provides a cotton bollworm salivary protein Ha22. The amino acid sequence of the cotton bollworm salivary protein Ha22 is as shown in SED ID NO. 1. Transient expression of the Ha22 protein on tobacco can cause tobacco cell necrosis, stable overexpression of the Ha22 protein on cotton plants can induce cotton defensive gene response and positively regulate plant insect resistance, and the Ha22 protein has great application value for cotton insect-resistant breeding. In addition, the protein has the active characteristic of an elicitor, so that the purpose of green prevention and control can be achieved by spraying whole protein to plants through in-vitro expression and inducing plant resistance.

The invention discloses a method for collecting mosquito saliva protein and viruses. The invention provides a method for collecting protein and / or virus in saliva of mosquitoes. The method comprises the following steps: collecting saliva, separating saliva, washing, and collecting protein and / or virus. According to the method provided by the invention, a relatively large amount of mosquito salivary protein can be collected in a short time without causing any harm to mosquitoes, the mosquito salivary protein of the same mosquito can be collected at multiple time points, the utilization rate of the mosquitoes is remarkably improved, dynamic collection can be realized, special materials or equipment are not needed, and the cost is low. Therefore, the applicability is further improved.

The invention provides methods for diagnosing degenerative joint disease in an animal by collecting a saliva sample from an animal; determining the amount of at least one diagnostic agent selected from the group consisting of interferon gamma, interferoninducible protein-10, interleukin-2, and total saliva protein in the saliva sample; comparing the amount of diagnostic agent found in the saliva sample to a corresponding amount of the same diagnostic agent found in a saliva sample from one or more comparable control animals that do not suffer from degenerative joint disease; and diagnosing that the animal is susceptible to or suffering from degenerative joint disease if the amount of diagnostic agent in the saliva sample from the animal is greater than the amount of same diagnostic agent found in the saliva sample from the one or more comparable control animals.

The invention provides silanization modified zirconium oxide powder, a cleaning agent and a preparation method of the silanization modified zirconium oxide powder, surface grafting groups of the silanization modified zirconium oxide powder comprise amino groups and epoxy groups, and the cleaning agent is alkaline and contains the silanization modified zirconium oxide powder. A large number of amino groups and epoxy groups are grafted on the surface of the silanization modified zirconium oxide powder, the amino groups can generate charge adsorption with salivary protein in an alkaline environment so as to weaken the binding force between a protein film and a dental restoration, and the epoxy groups are activated in the alkaline environment so as to open a ring and generate hydroxyl groups, so that the silanization modified zirconium oxide powder can be used for preparing the dental restoration. According to the invention, silanized zirconium oxide is added into a dental restoration, so that a Zr-O-P bond between a protein membrane and the restoration made of the zirconium oxide material is broken, the affinity of Zr < 4 + > in the silanized zirconium oxide to a phosphate group in saliva is higher than that of the restoration made of the zirconium oxide material, and finally, the cleaning agent can effectively clean saliva and blood remaining on the dental restoration.

Provided are nucleoside-modified mRNA molecules encoding tick salivary antigens, and LNP vaccines comprising the mRNA molecules and methods of use thereof to treat or prevent diseases or disorders associated with a tick-borne pathogen including Lyme disease.

The invention belongs to the technical field of biology, and particularly relates to a screening method of a small molecule compound for inhibiting insect-borne diseases and related application. In order to solve the problem that crop production is seriously harmed due to efficient aphid virus transmission, starting from the aphid feeding and virus obtaining process, a virtual screening-molecular docking method is adopted, Chinese gall bodies interacting with aphid colloidal salivary protein sheath protein are screened out for tanning, the Chinese gall bodies are used for tanning, viruses attached to the tip of an aphid mouth needle are reduced, and the aphid yield is improved. And the transmission efficiency of the virus is reduced.

The invention discloses dsRNA (double-strand ribose nucleic acid) capable of inhibiting wheat aphid salivary protein MYS2 gene expression and application thereof. The invention provides the double-stranded RNA molecule shown in the sequence 2 in a sequence table. The DNA (deoxyribonucleic acid) molecule encoding the RNA molecule and the application of the RNA molecule or DNA molecule are protected in the invention, and the application of RNA molecule or DNA molecule includes at least one of the following: (1) preventing and treating aphid; (2) promoting death of aphid; (3) inhibiting growth of aphid; and (4) inhibiting expression of in vivo salivary protein MYS2 gene of aphid. The dsRNA has important application value in prevention and control of aphid in agriculture production.