Lectin chip for detecting carbohydrate chain markers based on blood serum and commonly based on protein in blood serum and saliva as well as kit and application of lectin chip

A lectin and marker technology, applied in the field of lectin chips, can solve the problems of aggravation, reduction, and unreported

Inactive Publication Date: 2016-06-15
NORTHWEST UNIV
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  • Abstract
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Problems solved by technology

[0009] At present, the research on glycosylation is mostly reported in other liver diseases (hepatitis B, cirrhosis, liver cancer). For example, the level of Gal-type double-antenna type N-glycan chain in serum of patients with liver fibrosis after hepatitis B virus (HBV) infection is significantly higher than that of healthy people Volunteers, at the same time, the levels of double-antennary and t

Method used

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  • Lectin chip for detecting carbohydrate chain markers based on blood serum and commonly based on protein in blood serum and saliva as well as kit and application of lectin chip
  • Lectin chip for detecting carbohydrate chain markers based on blood serum and commonly based on protein in blood serum and saliva as well as kit and application of lectin chip
  • Lectin chip for detecting carbohydrate chain markers based on blood serum and commonly based on protein in blood serum and saliva as well as kit and application of lectin chip

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Embodiment Construction

[0027] The present invention will be further described by the specific examples given below, but not as a limitation of the present invention.

[0028] In the present invention, the lectins shown in Table 1 are used for testing.

[0029] Table 1

[0030]

[0031]

[0032]

[0033] The equipment used includes: electric blast drying oven: Tianjin Test Company; autoclave: Japan TOMY Company; ultra-speed refrigerated centrifuge 5804R: Germany Eppendorf Company; trace nucleic acid protein analyzer: Germany Implen Company; biochip scanner 4000B: American Axon Company; chip spotting instrument: Boao Jingxin SmartArrayer48 spotting instrument; chip hybridization box HL-2000: American UVP company.

[0034] The specific test steps are as follows:

[0035] (1) Study population and whole serum / saliva collection

[0036] The volunteers had no other diseases and did not take any medicine within a week. There were 40 patients with ACHBLF, 30 patients with cHB, and 80 patients with...

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Abstract

The invention provides a lectin chip for detecting carbohydrate chain markers based on protein in blood serum or saliva as well as a kit and application of the lectin chip, relates to the lectin chip for detecting the carbohydrate chain markers in blood serum or saliva, and particularly relates to the lectin chip for detecting the carbohydrate chain markers based on protein in blood serum and saliva as well as a kit and application of the lectin chip. The invention aims at providing a non-injury lectin chip for detecting the carbohydrate chain markers by identifying protein in saliva or blood serum and a method thereof. The lectin chip for detecting the carbohydrate chain markers based on serum carbohydrate binding protein, provided by the invention, comprises a testing lectin probe set, wherein the testing lectin probe set at least comprises PTL-I, PNA, AAL, LTL, STL, BS-I, PTL-II, SBA, ACA, UEA-I, MAL-I and PHA-E+L. The lectin chip can be used for rapidly detecting a specific carbohydrate protein chain structure in blood serum and saliva samples, and rapidly judging the specific combination of carbohydrate chains in the samples, so as to determine whether corresponding people have hepatitis or not and determine whether people are ACHBLF patients or not.

Description

technical field [0001] The invention relates to a lectin chip for detecting sugar chain markers, in particular to a lectin chip for detecting sugar chain markers based on serum and jointly based on proteins in serum and saliva and an application thereof. Background technique [0002] Severe hepatitis B refers to massive degeneration and necrosis of liver cells, liver failure, loss of liver biosynthesis, transformation, and detoxification functions, accumulation of metabolites in the body, and serious disturbance of the internal environment. Encephalopathy, obvious bleeding, or secondary infection, cerebral edema, acute renal failure, etc. Therefore, clinically, it has the characteristics of severe disease, rapid development, and dangerous onset. If it is not actively treated, the prognosis will be extremely poor. The pathogenesis of severe hepatitis may be related to the host's hyperactive immune response and the effect of endotoxin. Its incidence accounts for about 1% of h...

Claims

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Application Information

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IPC IPC(8): G01N33/96G01N33/68
CPCG01N33/96G01N33/68
Inventor 李铮钟耀刚张甲戌刘夏薇张志伟秦鑫敏舒健
Owner NORTHWEST UNIV
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