Lectin microarray for detecting carbohydrate chain marker based on protein in saliva, lectin microarray for detecting carbohydrate chain marker jointly based on protein in saliva and serum, kit and applications of lectin microarrays

Abstract

The invention discloses a lectin microarray for detecting a carbohydrate chain marker based on protein in saliva, a lectin microarray for detecting a carbohydrate chain marker jointly based on protein in saliva and serum, a kit and applications of lectin microarrays, and aims at providing a nondestructive lectin microarray for detecting a carbohydrate chain marker by identifying protein in saliva or serum and a method thereof.The lectin microarray for detecting the carbohydrate chain marker based on the carbohydrate-binding protein in saliva comprises a testing lectin probe set, and the testing lectin probe set at least comprises GSL-I, STL, ConA, SBA, ACA, WGA, PWM and SNA.By means of the lectin microarray, the structure of the glycoprotein carbohydrate chain specific in a saliva and serum sample can be rapidly detected, the specific binding of the carbohydrate chain in the sample can be rapidly judged, and whether corresponding people suffer from hepatitis or not is determined, and whether the people suffering from hepatitis are ACHBLF patients or not is determined.

Description

technical field [0001] The invention relates to a lectin chip for detecting sugar chain markers, in particular to a lectin chip for detecting sugar chain markers based on saliva and based on proteins in saliva and serum and its application. Background technique [0002] Severe hepatitis B refers to massive degeneration and necrosis of liver cells, liver failure, loss of liver biosynthesis, transformation, and detoxification functions, accumulation of metabolites in the body, and serious disturbance of the internal environment. Encephalopathy, obvious bleeding, or secondary infection, cerebral edema, acute renal failure, etc. Therefore, clinically, it has the characteristics of severe disease, rapid development, and dangerous onset. If it is not actively treated, the prognosis will be extremely poor. The pathogenesis of severe hepatitis may be related to the host's hyperactive immune response and the effect of endotoxin. Its incidence accounts for about 1% of hepatitis B, mo...

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Problems solved by technology

[0009] At present, the research on glycosylation is mostly reported in other liver diseases (hepatitis B, cirrhosis, liver cancer). For example, the level of Gal-type double-antenna type N-glycan chain in serum of patients with liver fibrosis after hepatitis B virus (HBV) infection is significantly higher than that of healthy people Volunteers, at the same time, the levels of double-antennary and three-antennary N-glycan chains in serum decreased with the aggravation of liver fibrosis. 6) The level of N-glycan chains in Fucated biantennary type increased, while the level of N-glycan chains in triantennary type decreased, indicating that changes in the level of specific N-glycan chains in serum can be used to monitor the development of fibrosis, but in severe type B Not reported in hepatitis

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