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A lectin chip and kit for detecting sugar chain markers based on saliva and co-based on proteins in saliva and serum and its application

A lectin and marker technology, applied in the field of lectin chips, can solve problems such as unreported, reduced, aggravated

Active Publication Date: 2017-12-01
深圳格道糖生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, the research on glycosylation is mostly reported in other liver diseases (hepatitis B, cirrhosis, liver cancer). For example, the level of Gal-type double-antenna type N-glycan chain in serum of patients with liver fibrosis after hepatitis B virus (HBV) infection is significantly higher than that of healthy people Volunteers, at the same time, the levels of double-antennary and three-antennary N-glycan chains in serum decreased with the aggravation of liver fibrosis. 6) The level of N-glycan chains in Fucated biantennary type increased, while the level of N-glycan chains in triantennary type decreased, indicating that changes in the level of specific N-glycan chains in serum can be used to monitor the development of fibrosis, but in severe type B Not reported in hepatitis

Method used

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  • A lectin chip and kit for detecting sugar chain markers based on saliva and co-based on proteins in saliva and serum and its application
  • A lectin chip and kit for detecting sugar chain markers based on saliva and co-based on proteins in saliva and serum and its application
  • A lectin chip and kit for detecting sugar chain markers based on saliva and co-based on proteins in saliva and serum and its application

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Embodiment Construction

[0027] The present invention will be further described by the specific examples given below, but not as a limitation of the present invention.

[0028] In the present invention, the lectins shown in Table 1 are used for testing.

[0029] Table 1

[0030]

[0031]

[0032]

[0033]The equipment used includes: electric blast drying oven: Tianjin Test Company; autoclave: Japan TOMY Company; ultra-speed refrigerated centrifuge 5804R: Germany Eppendorf Company; trace nucleic acid protein analyzer: Germany Implen Company; biochip scanner 4000B: American Axon Company; chip spotting instrument: Boao Jingxin SmartArrayer48 spotting instrument; chip hybridization box HL-2000: American UVP company.

[0034] The specific test steps are as follows:

[0035] (1) Study population and whole serum / saliva collection

[0036] The volunteers had no other diseases and did not take any medicine within a week. There were 40 confirmed ACHBLF patients, 30 cHB patients, and 80 HC patients....

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Abstract

A lectin chip and kit for detecting sugar chain markers based on saliva and jointly based on proteins in saliva and serum and its application, the lectin chip for detecting sugar chain markers, specifically relates to a saliva-based and jointly based on saliva and lectin chips for detecting sugar chain markers in serum proteins and their applications. The purpose is to provide a non-damaging lectin chip and method for detecting sugar chain markers by identifying proteins in saliva or serum. The present invention provides a lectin chip for detecting sugar chain markers based on sugar-binding proteins in saliva, including a test lectin probe set, the test lectin probe set at least including GSL-I, STL, ConA, SBA, ACA , WGA, PWM, SNA. The lectin chip can quickly detect the unique sugar chain structure of glycoproteins in saliva and serum samples, quickly determine the specific binding of sugar chains in the sample, determine whether the corresponding population has hepatitis, and determine whether they are ACHBLF patients.

Description

technical field [0001] The invention relates to a lectin chip for detecting sugar chain markers, in particular to a lectin chip for detecting sugar chain markers based on saliva and based on proteins in saliva and serum and its application. Background technique [0002] Severe hepatitis B refers to massive degeneration and necrosis of liver cells, liver failure, loss of liver biosynthesis, transformation, and detoxification functions, accumulation of metabolites in the body, and serious disturbance of the internal environment. Encephalopathy, obvious bleeding, or secondary infection, cerebral edema, acute renal failure, etc. Therefore, clinically, it has the characteristics of severe disease, rapid development, and dangerous onset. If it is not actively treated, the prognosis will be extremely poor. The pathogenesis of severe hepatitis may be related to the host's hyperactive immune response and the effect of endotoxin. Its incidence accounts for about 1% of hepatitis B, mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/68G01N2800/085
Inventor 李铮钟耀刚张甲戌刘夏薇张志伟秦鑫敏舒健
Owner 深圳格道糖生物技术有限公司
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