70 results about "Severe disease" patented technology

Compositions and methods are provided for prognostic classification of autoimmune disease patients into subtypes, which subtypes are informative of the patient's probability of developing clinical symptoms or severe disease. The patterns of circulating blood levels of serum cytokines provides for a signature pattern that can discriminate patients that have a high probability of developing disease from those that have a low probability of developing disease. Assessment of this signature pattern in a patient thus allows improved methods of care and intervention. In one embodiment of the invention, the autoimmune disease is rheumatoid arthritis.

The embodiment of the invention provides a disease prediction method and device, a medium, and electronic equipment. The method comprises the steps: obtaining the data, correlated with health, of a plurality of users; carrying out the clustering analysis of the plurality of users based on the data, correlated with the health, of the users, so as to dividing the users into at least one class; determining a target class where a diagnosed patient belongs, and predicting other users in the target class as a high-risk group of diseases. According to the technical scheme of the invention, the methodcan achieve the prediction of the group which may have diseases, can guarantee the timely finding of severe diseases, so as to find and treat a disease early, and improve the level of health management of users.

The invention provides a field first-aid unit which comprises a box body. A first-aid medical equipment informatization management module is arranged in the box body, wherein the box body is a knapsack-type box body; a lifting handle or a strap is arranged outside the box body; the first-aid medical equipment informatization management module is provided with an information management module, a vital sign monitoring module, a defibrillator module, a first-aid respirator module, an ultrasonic module and an automatic cardiopulmonary resuscitation instrument module; at least one group of first sockets is arranged on a face cover of the first-aid unit; the first sockets are electrically connected with at least one of the vital sign monitoring module, the defibrillator module, the first-aid respirator module, the ultrasonic module and the automatic cardiopulmonary resuscitation instrument module; a communication module is arranged in the information management module; the communication module transmits and receives a wireless signal by a mobile communication network. The field first-aid unit provided by the invention has the characteristics that functions of a mobile ICU (Intensive Care Unit) are realized, carrying is convenient, the use is easy, safe and reliable, peacetime and emergency are combined, and provides a reliable approach for field rescue of the wounded suffering from urgent severe diseases.

The invention relates to a special fertilizer for overcoming Chinese-drug pseudo-ginseng continuous cropping obstacles. The fertilizer is formed by mixing rapeseed dregs, pig manure, active carbon, bentonite and composite nutrient. The fertilizer is specially developed to solve the problems of low rate of emergence and severe disease damage of pseudo-ginseng after the continuous cropping, finally resulting in output reduction and even total crop failure. The invention can supplement nutritive elements required for the growth of the pseudo-ginseng, improve the physicochemical properties of the continuous cropping soil, effectively adsorb autotoxic substances of the pseudo-ginseng in the soil, improve the micro-ecological environment of the soil and enhance the stress resistance of pseudo-ginseng plants, promote the normal growing development of the pseudo-ginseng plants, thereby finally greatly increasing the yield of the continuous cropped pseudo-ginseng. The invention has obvious ecological effects and economic benefits.

The invention discloses an application of Bacillus methylotrophicus ZBL-1 in control of cotton verticillium wilt. The Bacillus methylotrophicus ZBL-1 with CGMCC No.11636 and higher inhibition effect on verticillium dahlia is obtained through separation, screening, breeding and domestication of soil samples collected in the cotton fields with severe diseases in Shihezi city and is obviously different from common Bacillus methylotrophicus strains, and meanwhile, the control effect of the screened strain ZBL-1 on the cotton verticillium wilt in indoor pot culture experiments is 73.0%; the control effect of the screened strain ZBL-1 in field plot experiments is 43.1%, and whether in development of a new biopesticide or biological control agent, the Bacillus methylotrophicus ZBL-1 as a biological control material for the cotton verticillium wilt has very good application prospect and has realistic significance and functions.

The invention relates to a compound sulfamonomethoxine sodium injection for veterinary use and a preparation method thereof, and belongs to the technical field of medicines for veterinary use. The raw material components of the compound sulfamonomethoxine sodium injection for veterinary use comprise sulfamonomethoxine sodium, dexamethasone sodium phosphate, trimethoprim, imidocarb, astragalus polysaccharide, organic solvent and water for injection. As a special compound preparation for veterinary use, the injection has remarkable efficacy on strengthening resistance of livestock and poultry, and treating mixed infection and severe diseases caused by a plurality of pathogenic bacteria and viruses. The injection has the advantages of convenient use, short treatment course, low drug resistance and the like.

The invention discloses a rooting-promoting, disease-resistant and plant-strengthening agent for cotton and a water, fertilizer and pesticide integrated application method. The rooting-promoting, disease-resistant and plant-strengthening agent is mainly prepared from a growth regulator, a microelement, a signal molecule donor and an elicitor capable of improving the disease resistance of the cotton, wherein the growth regulator is prepared from indolebutyric acid, sodium nitrophenolate, naphthylacetic acid and diethyl aminoethyl hexanoate through compounding, the microelement is prepared from zinc sulfate, ferrous sulfate, ammonium molybdate, amino acid powder and ammonium hydrogen phosphate through compounding, the signal molecule donor is NO donor sodium nitroprusside, and the elicitor is a penicillium dry mycelium extracting solution. The problems that a drip irrigation technology does no good to root development and severe diseases are caused by successive cropping to the cotton are solved, the growth regulator accelerates growth of root systems, the physiological function time of the root systems is prolonged, the disease-resistant elicitor enhances the resistance of cotton plants, growth of lateral roots is further promoted in combination of the signal molecule donor, and the disease-resistant response of the cotton plant is promoted. The rooting-promoting, disease-resistant and plant-strengthening agent can promote growth of roots and resist diseases in multiple angles; the agent can be injected into irrigation water through a fertilizer pump, integrated application of water, fertilizer and pesticides in the drip irrigation cotton field of the northwest inland is realized, and the agent can promote growth of roots, resist diseases and strengthen seedlings and has remarkable disease resisting and yield increasing effects.

The invention relates to the field of medical apparatuses, and particularly discloses a medical clinical fixing device for medical treatment of a severe disease. Aiming at the problem that an existingmedical treatment bed cannot effectively fix a patient, the clinical fixing device is provided. The clinical fixing device comprises a bottom plate, a supporting pillar is connected to the top of thebottom plate, a bed board is connected to the top of the supporting pillar, and two symmetric slide grooves are formed in one side of the top of the bed board; slide blocks are slidably connected tothe interiors of the slide grooves, a U-shaped positioning board is connected between the tops of the two slide blocks, a first mounting plate and a second mounting plate are movably mounted at the other side of the bed board separately, and a first rack board and a second rack board are connected to the bottoms of the first mounting plate and the second mounting plate respectively. By means of the device, the legs and the upper body of a patient with the severe disease can be simultaneously limited and fixed, the body of the patient can also be fixed in the vertical direction, the situation that the patient shakes about due to restlessness is prevented, the efficiency of a clinical operation can be effectively improved, and the clinical fixing device is suitable for popularization.

The invention discloses a sterilization / endotoxin neutralization polypeptide modified by polyethylene glycol, which consists of a formula as follows: X-Y-BNEP-OH or H-BNEP(i-Y-X)-OH, wherein, X is polyethylene glycol or single-methoxy polyethylene glycol, Y is a linking group, i is 1 or 3 or 13 or 14, representing the position number of lysine with a modified on side-chain amino group in an amino acid sequence; and BNEP is the sterilization / endotoxin neutralization polypeptide which has an amino acid sequence shown by SEQ ID No.1; the modification polypeptide, while maintaining the endotoxin neutralization activity of BNEP, prolongs the half-life in vivo of BNEP, and shows potential for being developed into new endotoxin neutralization medicaments, thus being capable of providing new curative medicaments for treating patients of severe diseases such as pyaemia, septic shock and the like. The invention also discloses a preparation method of the modification polypeptide, which has high product yield and high purity, thus being capable of realizing fixed-point modification of polyethylene glycol of synthetic polypeptide.

The invention relates to the field of agricultural biotechnology and provides a method for cultivating oncidium, aiming at solving the problems such as rooting difficulty, weak reproductive capacity, low growth speed, long growth period, severe diseases in seedling hardening and transplanting stages, low bottle-out survival rate and weak stress resistance existing during oncidium production. The method for cultivating oncidium comprises the following steps: (1) culturing oncidium tissue culture seedlings by an MS culture medium; (2) culturing Piriformospora indica fungi by a solid PDA culture medium; (3) co-culturing the oncidium and the Piriformospora indica fungi; and (4) performing bottle-out cultivation on the oncidium tissue culture seedlings. According to the cultivation method disclosed by the invention, the disease rate during oncidium bottle-out seedling transplanting is reduced, and the stress resistance is improved.

The invention relates to a compound doxycycline hydrochloride injection for veterinary use and a preparation method thereof. The raw materials comprise doxycycline hydrochloride, thiamphenicol, dexamethasone sodium phosphate, trimethoprim, diclofenac sodium, organic solvent and water for injection. As a special compound preparation for veterinary use, the injection has obvious curative effects on infectious diseases caused by gram-positive bacteria, gram-negative bacteria and mycoplasma, quickly takes effects after the injection is injected into livestock with the diseases, and has special efficacy on mixed infection and severe diseases caused by various pathogenic bacteria and viruses. After intramascular for animals, the injection is easily absorbed, takes effect for a long time and causes little local stimulation. The injection has the advantages of convenient use, short treatment course, low drug resistance, and the like.

The invention provides a variable-rate spraying plant protection unmanned aerial vehicle equipped with variable-concentration pesticide preparation and pesticide application modules. The variable-ratespraying plant protection unmanned aerial vehicle comprises an unmanned aerial vehicle module, a vehicle-mounted power supply module, a vehicle-mounted control module, the vehicle-mounted pesticide preparation module and the vehicle-mounted pesticide application module. Compared with the prior art, the variable-rate spraying plant protection unmanned aerial vehicle has the following advantages: (1) effectively coping with big differences in disease index in different areas of an operation land block, following the principle of ''mild pesticides for mild disease and severe pesticides for severe disease'' to achieve ''variable-concentration'' pesticide application, and making up for the technical deficiencies of a ''variable-rate'' pesticide application unmanned aerial vehicle; (2) achieving the ''real-time and on-demand'' preparation of pesticide solutions with different concentrations during the flight operation of the plant protection unmanned aerial vehicle by adopting the two-tankindependent control output design with a water tank and a pure pesticide tank, and achieving the recovery and recycling of the remaining water and pesticides after the operation; (3) adopting a noveldesign of using a single-chip microcomputer to control two different mediums to perform the preparation of the pesticide solutions with different concentrations, achieving the advantages of good system responsiveness, high concentration accuracy and the like, and facilitating the popularization and application.

The invention provides methods and materials for diagnosing a rheumatoid arthritis condition in a patient. Specifically, the invention provides methods and materials for classifying a rheumatoid arthritis condition as diffuse, follicular, or granulomatous. In addition, the invention provides methods and materials for determining if an individual suffering from a rheumatoid arthritis condition will develop severe disease.

The invention discloses a preparation method for bird feed. The bird feed is composed of pork liver powder, pig bone powder, cow bone powder, miscellaneous bone powder, miscellaneous fish powder, soy-bean cake powder, small red bean powder, sweet potato powder, pumpkin powder, pine nut powder, water spinach powder, sugarcane powder, rape powder, earthworm powder, akebia stem powder and cortex periplocae powder according to a certain percentage which is the weight percentage. The bird feed is low in production cost, raw materials are easy to obtain, the recipe and the preparation method are simple, the raising effect is good, raised birds grow fast and have robust and beautiful bodies, hair of the birds has bright colors and is colorful, the disease incidence rate of the birds is low, and the defects that when certain breeders or large breeding farms raise the birds, due to nutritional deficiency, the hair of the birds fall, the birds peck the hair, grow slowly, do not like tweeting, lack movement and are prone to diseases, the color of the hair is dark and not shiny, feathers of the birds are loose and untidy, and the birds which have severe diseases are prone to death are effectively overcome.

The invention relates to a holographical ear acupoint treatment device controlled by a computer program, which belongs to the field of medical devices, and is installed in holographical ear acupoints of a program of a practical general practitioner diagnosis and treatment system controlled by a multimedia computer to treat related diseases. A computer software system of the device has eight functions with strong practicality, namely a function of critical disease expert differential diagnosis, a function of comprehensive treatment, a function of foetus education for foetus, a function of prescription, clinical medical advice and old-young medicament dosage conversion, a function of critical severe disease rescue success and death grading, a function of various detections of outpatients and outpatient case history management, a function of normal value inquiring of common clinical laboratory examination, and a function of medical text editing and the like. The system is also characterized in that a system for differentiating and diagnosing cerebral hemorrhage, cerebral thrombosis and new born critical diseases, and can carry out early-stage differentiation and diagnosis on the cerebral hemorrhage and the cerebral thrombosis.

The invention discloses a composition capable of improving immunity. The composition is prepared from the following raw materials in parts by weight: 1 to 4 parts of corn cervi pantotrichum, 1 to 3 parts of colla corii asini, 2 to 4 parts of jujube, 3 to 5 parts of fructus schisandrae, 1 to 3 parts of lycii fructus, 1 to 3 parts of rhizoma dioscoreae, 3 to 5 parts of semen sesami nigrum, 3 to 5 parts of fructus mori, 1 to 3 parts of jujube peel, 1 to 3 parts of semen cuscutae, 1 to 4 parts of radix ginseng Rubra, 2 to 8 parts of radix astragali seu hedysari, 1 to 4 parts of jujube kernel, 3 to 5 parts of poria cocos, 2 to 4 parts of liquorice root and 2 to 4 parts of Chinese angelica. The traditional Chinese medicine composition has a remarkable effect of obviously improving the immunity of the human body. On the basis of the effect, the traditional Chinese medicine composition can be used for clinically treating severe diseases troubling the human beings for a long term such as multi-organ tumor, nephritis, uremia, diabetes and hepatitis, and the application effectiveness reaches more than 95 percent.

The invention provides a traditional Chinese medicine for treating pulmonary tuberculosis, which is an orally taken medicine mainly prepared from twelve traditional Chinese medicinal ingredients such as dandelion, Chinese violet, honeysuckle flowers, rehmannia glutinosa and the like. In order to get better efficacy, a plaster for external application is prepared from dragon blood, calomel, verdigris, frankincense, myrrh, kenaf, rosin and borneol on the basis of the orally taken medicine. The medicine has the efficacy in astringing and softening tissue, easing pain, promoting metabolism, replenishing Qi, stanching bleeding and reducing swelling, and has good effect of restoring physical inside. If the medicine is aided with the plaster for external application, the efficacy in drawing out poison, removing necrosis, promoting granulation and treating both inside and outside can be promoted. Through the statistics on more than 200 patients, the medicine has the total effective rate up to 100 percent and the cure rate over 98 percent; the patients in slight disease conditions can be cured after taking the medicine for 1 to 2 months; and the patients in severe disease conditions can be cured after taking the medicine for 3 to 6 months. The medicine has the advantages of no toxic-side effect, no adverse reaction, easy absorption, high cure rate and relapse insusceptibility after recovery.

The invention provides a transferring system for patients suffering from acute and severe diseases. The system comprises a chassis and a bedstead mounted on the chassis, the four corners of the bottomof the chassis are provided with universal wheels, and the chassis is provided with an oxygen cylinder bracket, a brake pedal and a side pedal; foldable railings which can be in a locked state underthe effect of gravity are arranged above the two sides of the bedstead, foldable push handles which can be in a locked state under the effect of gravity are arranged on the two sides of one end of thebedstead respectively, and the other end of the bedstead is provided with an overturning hidden instrument holder for placing a first aid instrument. The transferring system has multiple functions, the railings, the push handles and the instrument holder can be pulled out / up when in use and folded when not in use, the operation is simple in the folding process, operation can be conducted with onehand, and the time is saved. The transferring system is reasonable in structural arrangement, flexible, changeable, firm in structure and safe to use, medical staff can selectively utilize the different functions of the system according to the usage needs of different patients, the space is saved, the first aid efficiency is improved, and convenience is provided for both doctors and patients.

The invention relates to a bearing branch regeneration and invigoration method for a green prickleyash tree and belongs to the technical field of planting of economic crops. The method comprises the following three steps of improving the underground environment, improving the ground environment and performing flower and fruit protection measures. By virtue of the method provided by the invention, the weakened tree vigor problems such as trunk decaying, disorderly and over crowded crowns, crossed and overlapped branches, terminal shoot wilt, withered branches and leaves, severe disease and insect damage and root system undergrowth of the green prickleyash tree with tree age not less than 15 years can be thoroughly solved, so that the bearing branch vitality is restored; the regenerated and invigorated green prickleyash tree has the advantages that new branches bear fruits in the next year, the tree vitality is restored after three years, and furthermore the tree body is shortened. In addition, the newly fruited green prickleyash is completely full in fruits, increased in oil vacuole and improved in quality, and the yield of per 667m can be improved by at least 30%.

The invention discloses an oxiracetam composition with dehydrating and urination promoting effects and application thereof. The composition is mainly prepared from the following 1,000mL of raw materials in total: 160-200g of oxiracetam, 8.5-9.5g of sodium chloride, and the balance of water for injection. The composition can take effects of dehydrating and promoting urination quickly, has a long-lasting action of reducing intracranial pressure, is convenient to use, can pass through blood-brain barrier, but not enter brain cells, thereby avoiding increase of intracranial pressure. The composition is convenient in administration, does not crystallize at room temperature, and is suitable for sudden-onset disasters at ordinary times and cold regions, low temperature, wild field and other harsh environments in wartime. The composition can be rapidly discharged via kidneys to generate good urination promoting effect, has no renal function damage and other side effects, and has good water solubility, high solubility, no toxicity, high safety and no tolerance or accumulated side effects after repeated administration. The composition can be used for emergency and severe disease rescue, improving brain metabolism, promoting consciousness, and accelerating recovery of cognition and limb function after injuries, and has multiple-effect characteristic.

The invention relates to a pepper planting method for reducing diseases and insects, wherein the method belongs to the field of agricultural planting technology. The method comprises the steps of performing breeding soil fermentation; performing seed disinfection, insect killing and germination accelerating; kneading nutrition blocks; performing seedling growing; selecting and processing a planting field: namely selecting the field which has sufficient sunshine, no water accumulation and is used for planting flue-cured tobacco or tobacco last year, in November-December, flatly laying flue-cured tobacco stems or tobacco stems on the base layer of the planting field, flatly laying straws, branches and leaves or / and weeds on the surface layer, wherein the thickness is 3-5cm, setting fire to the combined layer for burning, and after burning, scattering lime; performing transplanting; and performing disease and insect controlling, namely in flowering and fruiting of the pepper, performing insect killing on the branches and leaves of the pepper by means of a sprayer for trichlorfon with concentration of 1-2%. The method of the invention can effectively settle a problem of pepper output reduction caused by low emergence rate caused by severe diseases and insects, undeveloped root system, low transplanting survival rate caused by easy biting and breakage by insects, easy disease in growing process, insect plague, etc.

The invention relates to a method for improving an old vegetable field. Due to years of vegetable planting, garden soil aging, descending land capability and increasingly severe disease and pest at present, the economic benefit per unit area descends. The method is realized in the following steps: applying more farmyard manure, increasing humus, adopting different fertilizers, and planting different families (species) of vegetables in a crop rotation mode to improve the soil and avoid shortage of a certain element in soil; and in addition, intermingling high and low plants in the vegetable field, thereby improving the soil, reducing the disease and pest and increasing the planting benefit. The ditches around the vegetable garden must be matched smoothly, thereby facilitating drainage and irrigation, lowering the groundwater level and benefiting the well growth of the root system. Meanwhile, the management techniques, such as ground surface cover and the like, are adopted to prevent water and soil erosion, cultivate the aftereffect of soil and strive for good harvest all the year.

The invention discloses a method for treating fruit trees with severe diseases and insect pests through root cutting and liquid absorbing. The method comprises the steps that healthy root systems of fruit trees are excavated and cut off, pesticide liquid prepared according to pesticide needed by fruit tree preventing and treating objects is placed into bottles, the cut root systems are placed in the bottles, and the root systems naturally absorb pesticide liquid to pathogenic parts. Consequently, the purposes of treating and preventing are achieved. The method breaks through the traditional technology of preventing diseases and insect pests through spraying pesticide, is improved in the mode that the root systems directly absorb the pesticide liquid to the disease and insect pest occurring parts, can kill pests and germs timely and effectively, has remarkable effect on diseases and sect pests hard to prevent and treat, and saves pesticide by 50% than the traditional pesticide spraying technology.

The invention discloses a method of enriching and detecting miRNA in a sample on the basis of a magnetic bead coupled antibody, and a matched microfluidic apparatus. The method herein includes the following steps: 1) connecting an anti-Ago2 monoclonal antibody to magnetic beads to obtain anti-Ago2 immunomagnetic beads; 2) mixing the anti-Ago2 immunomagnetic beads with a sample to enrich an Ago2-miRNA composite through an antigen-antibody immuno-reaction; 3) by means of a lysis liquid, carrying out high-temperature transformation to the enriched Ago2-miRNA composite so as to release miRNA for subsequent quantitative analysis. In the method, by means of combination of high-specificity of antigen-antibody immunological recognition and combination, simplicity and operability of high-affinity magnetic bead separation and high-effective reaction and enrichment properties of the microfluidic apparatus, various miRNAs, which are related to diseases, especially tumors, in bio-samples, such as blood, urine, saliva, synovial fluid, cell lysis liquid and the like, can be quickly detected. The method has important significance on early and accurate diagnosis of clinically severe diseases.

The invention relates to a pharmaceutical composition containing Ilaprazole or a salt thereof and a preparation method thereof. The pharmaceutical composition contains or consists of Ilaprazole or a salt thereof and a Ilaprazole derivative, wherein the amount of the Ilaprazole derivative is not higher than 1.3wt% (less than 1.3wt%). The pharmaceutical composition can be used for treating peptic ulcer bleeding and stress ulcer and preventing upper gastrointestinal hemorrhage caused by severe diseases.

The invention discloses an intelligent medical device access method based on big data. The big data used by an intelligent medical system based on the big data comprise a medical record big data module, a medical device big data module, a doctor big data module, a disease diagnosis big data module and a device monitoring big data module. Diseases are classified according to incidence rates and severities, a disease in a high incidence rate and a medical device needed by a severe disease are preferentially accessed to the intelligent medical system, other medical devices adopt a random competition mode to be accessed to the intelligent medical system, and the preferential conditions are determined according to big data analysis. The method of the invention adopts medical big data to match patients and medical resources, the waiting time of a patient in a high incidence rate and a severe patient is reduced, the medical accident probability is reduced, and the method of the invention is applicable to intelligent medical treatment.

The invention discloses markers and a kit for implementing screening, diagnosis and therapeutic efficiency evaluation of chronic periodontitis. The invention discovers the molecular markers which arerelated to diagnosis and severe disease course classification of chronic periodontitis, wherein the molecular markers include p19mRNA, p40mRNA, p35mRNA and EBI3mRNA, as well as quantitative detectionof the molecular markers and a combination of the molecular markers. The markers, which are applicable to diagnosis of the chronic periodontitis, are expected to be used for preparing the kit for implementing screening, diagnosis and therapeutic efficiency evaluation of the chronic periodontitis.

The invention discloses a method for planting ginger in a greenhouse. The method includes the steps of greenhouse building, planting, heating, ground temperature adjustment control, irrigation and the like. According to the method, temperature, especially soil temperature, can be adjusted according to different growth stages of ginger, and soul humidity and air permeability can be effectively controlled, so that normal growth of ginger in the greenhouse is ensured, severe diseases are avoided, scientific and reliable greenhouse planting of ginger is achieved, cost is low, benefits are high, and large-scale popularization is achieved.

The invention discloses a metal doped ferrite nano material and a preparation method of magnetic nano particles containing the same and application and products thereof. The metal doped ferrite is a metal doped iron oxides with the chemical general formula of MxFe3-xO4, wherein M represents metal elements selected from a VIIB group, a VIII group or a IIB group, X represents the concentration rangeof doping metal, and 0<x<=4. A Fe-Au nano composite material is applied to magnetic resonance imaging so that a high-quality MRI contrast medium with significantly excellent imaging performance, highmagnetic sensitivity and abundant MR T1 and T2 weight imaging signals, the distinguishing and inspecting of severe diseases of tumors, cardiovascular diseases, neural system diseases, skeleton musclediseases and the like can be improved, and therefore the error of medical imaging inspecting and the treatment cost are significantly reduced.

The present invention provides methods for diagnosing an immune-mediated disease, e.g., an autoimmune disease, an allergy or an inflammatory disease. Diagnosis is made by detecting a heterozygous or homozygous genotype of haptoglobin 2 or by detecting and quantifying pre-haptoglobin 2 mRNA or protein. After diagnosis, the disease may be treated by decreasing cell permeability leading to increased transepithelial electrical resistance, for example, by administering an antibody directed against single chain zonulin thereby inhibiting epidermal growth factor receptor and inhibiting proteinase-activated receptor 2 (PAR2). Also provided is a single step method for determining severity of an immune-mediated disease in a subject by identifying a genotype for haptoglobin 2 in a biological sample from the subject. A homozygous genotype correlates to 2 copies of zonulin and a more severe disease than a heterozygous genotype correlating to 1 copy of zonulin.